May 20, 2019 Thomas H. Adams Chief Executive Officer Trovagene, Inc. 11055 Flintkote Avenue San Diego, CA 92121 Re: Trovagene, Inc. Post-Effective Amendment No. 3 to Form S-1 Filed May 17, 2019 File No. 333-224808 Dear Mr. Adams: We have reviewed your post-effective amendment and have the following comment. Please respond to this letter by amending your registration statement and providing the requested information. If you do not believe our comment applies to your facts and circumstances or do not believe an amendment is appropriate, please tell us why in your response. After reviewing any amendment to your registration statement and the information you provide in response to this comment, we may have additional comments. Post -Effective Amendment No. 3 to Form S-1 General 1. Please revise to include a complete prospectus that includes all of the disclosure items required by Part I. Refer to Securities Act Rule 472(b). We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Thomas H. Adams Trovagene, Inc. May 20, 2019 Page 2 You may contact Jeffrey Gabor at 202-551-2544 or Mary Beth Breslin at 202-551-3625 with any questions. Sincerely, FirstName LastNameThomas H. Adams Division of Corporation Finance Comapany NameTrovagene, Inc. Office of Healthcare & Insurance May 20, 2019 Page 2 cc: Jeffrey Fessler, Esq. FirstName LastName