United States securities and exchange commission logo June 12, 2020 Joshua Disbrow Chief Executive Officer Aytu BioScience, Inc. 373 Inverness Parkway, Suite 206 Englewood, Colorado 80112 Re: Aytu BioScience, Inc. Registration Statement on Form S-3 Filed June 8, 2020 File No. 333-239010 Dear Mr. Disbrow: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Alan Campbell at 202-551-4224 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Anthony Epps