United States securities and exchange commission logo September 10, 2020 Charles N. Funk Chief Executive Officer MidWestOne Financial Group, Inc. 102 South Clinton Street Iowa City, Iowa 52240 Re: MidWestOne Financial Group, Inc. Registration Statement on Form S-4 Filed September 3, 2020 File No. 333-248574 Dear Mr. Funk: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jessica Livingston at 202-551-3448 with any questions. Sincerely, Division of Corporation Finance Office of Finance