United States securities and exchange commission logo August 30, 2021 Alex Tarrabain Chief Financial Officer CEN Biotech, Inc. 300-3295 Quality Way Windsor, Ontario N8T 3R9 Re: CEN Biotech, Inc. Form 10-K for the fiscal year ended December 31, 2020 File No. 0-5557 Filed March 12, 2021 Dear Mr. Tarrabain: We have completed our review of your filing. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Sincerely, Division of Corporation Finance Office of Life Sciences