United States securities and exchange commission logo February 22, 2022 William Conkling Chief Executive Officer Rafael Holdings, Inc. 520 Broad Street Newark, NJ 07102 Re: Rafael Holdings, Inc. Registration Statement on Form S-3 Filed February 15, 2022 File No. 333-262754 Dear Mr. Conkling: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Stacie Gorman at 202-551-3585 with any questions. Sincerely, Division of Corporation Finance Office of Real Estate & Construction cc: Dov T. Schwell, Esq.