United States securities and exchange commission logo May 9, 2023 Daniel L. Florness President and Chief Executive Officer Fastenal Company 2001 Theurer Boulevard Winona, Minnesota 55987-1500 Re: Fastenal Company Form 10-K for Fiscal Year Ended December 31, 2022 Filed February 7, 2023 File No. 000-16125 Dear Daniel L. Florness: We have completed our review of your filing. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Sincerely, Division of Corporation Finance Office of Trade & Services