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                               September 11, 2023

       Jason Hanson
       Chief Executive Officer
       enGene Holdings Inc.
       7171 Rue Frederick Banting
       Saint-Laurent, QC H4S IZ9, Canada

                                                        Re: enGene Holdings
Inc.
                                                            Amendment No. 1 to
Registration Statement on Form S-4
                                                            Filed August 30,
2023
                                                            File No. 333-273851

       Dear Jason Hanson:

             We have reviewed your amended registration statement and have the
following
       comments. In some of our comments, we may ask you to provide us with
information so we
       may better understand your disclosure.

              Please respond to this letter by amending your registration
statement and providing the
       requested information. If you do not believe our comments apply to your
facts and
       circumstances or do not believe an amendment is appropriate, please tell
us why in your
       response.

              After reviewing any amendment to your registration statement and
the information you
       provide in response to these comments, we may have additional comments.
Unless we note
       otherwise, our references to prior comments are to comments in our
August 22, 2023 letter.

       Amendment No. 1 to Registration Statement on Form S-4

       Exhibit 5.1, page II-4

   1.                                                   We note assumptions (j)
and (k) in the legal opinion filed as Exhibit 5.1. Rather than
                                                        assuming conclusions of
law that are necessary for the opinion delivered in the legal
                                                        opinion filed as
Exhibit 5.2, counsel should revise to also provide an opinion that each of
                                                        the registrant and
enGene Inc. is validly existing, has the power to create the obligations
                                                        under the Warrant
Agreement and the Warrant Amendment Agreement and has taken the
                                                        required steps to
authorize entering into those agreement under the law of each such
                                                        entity's jurisdiction
of organization. For guidance, refer to Section II.B.1.f of Staff Legal
                                                        Bulletin No. 19 and
footnote 22 thereto.
 Jason Hanson
FirstName LastNameJason   Hanson
enGene Holdings Inc.
Comapany 11,
September NameenGene
              2023     Holdings Inc.
September
Page 2    11, 2023 Page 2
FirstName LastName
Exhibit 8.2, page II-4

2.       It appears that you have filed a "short-form" tax opinion as Exhibit
8.2. We note that
         counsel has opined that the discussion in the section of the
registration statement entitled
         "Material U.S. Federal Income Tax Consideration     Consequences to
U.S. Holders of
         New enGene Securities of the Ownership and Disposition of New enGene
Shares or New
         enGene Warrants" addresses the material U.S. federal income tax
consequences to U.S.
         Holders of New enGene Securities of the ownership and disposition of
New enGene
         Shares or New enGene Warrants (as such terms are defined in the
registration statement)
         and is accurate in all material respects. Please have counsel file an
opinion that revises
         this section to state clearly that the referenced disclosure is the
opinion of counsel, as
         opposed to indicating that the referenced disclosure addresses the
material U.S. federal
         income tax consequences and is accurate. For guidance, refer to
Section III.B.2 of Staff
         Legal Bulletin 19.
       You may contact Sasha Parikh at 202-551-3627 or Mary Mast at
202-551-3613 if you
have questions regarding comments on the financial statements and related
matters. Please
contact Doris Stacey Gama at 202-551-3188 or Tim Buchmiller at 202-551-3635
with any other
questions.



                                                               Sincerely,

                                                               Division of
Corporation Finance
                                                               Office of Life
Sciences
cc:      Howard A. Kenny, Esq.