United States securities and exchange commission logo January 2, 2024 Joseph Hagan Chief Executive Officer Regulus Therapeutics Inc. 4224 Campus Point Court, Suite 210 San Diego, CA 92121 Re: Regulus Therapeutics Inc. Registration Statement on Form S-3 Filed December 27, 2023 File No. 333-276287 Dear Joseph Hagan: This is to advise you that we have not reviewed and will not review your registration statement. Please refer to Rules 460 and 461 regarding requests for acceleration. We remind you that the company and its management are responsible for the accuracy and adequacy of their disclosures, notwithstanding any review, comments, action or absence of action by the staff. Please contact Jason Drory at 202-551-8342 with any questions. Sincerely, Division of Corporation Finance Office of Life Sciences cc: Asa Michael Henin