UNITED STATES
                       SECURITIES AND EXCHANGE COMMISSION
                             WASHINGTON, D.C. 20549

                                    FORM 8-K

                                 CURRENT REPORT
                     PURSUANT TO SECTION 13 OR 15(d) OF THE
                         SECURITIES EXCHANGE ACT OF 1934

        Date of report (Date of earliest event reported): March 31, 2006

                          Third Wave Technologies, Inc.

             (Exact Name of Registrant as Specified in Its Charter)

Delaware                               000-31745             39-1791034
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(State or Other Jurisdiction          (Commission           (IRS Employer
of Incorporation)                      File Number)          Identification No.)

502 South Rosa Road, Madison, Wisconsin                                53719
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(Address of Principal Executive Offices)                              (Zip Code)

                                 (608) 273-8933
                                 --------------
              (Registrant's Telephone number, including area code)

                                       N/A
                                      -----
          (Former Name or Former Address, if Changed Since Last Report)

Check the appropriate box below if the Form 8-K filing is intended to
simultaneously satisfy the filing obligation of the registrant under any of the
following provisions.

|_|   Written communications pursuant to Rule 425 under the Securities Act (17
      CFR 230.425)

|_|   Soliciting material pursuant to Rule 14a-12 under the Exchange Act (17 CFR
      240.14a-12)

|_|   Pre-commencement communications pursuant to Rule 14d-2(b) under the
      Exchange Act (17 CFR 240.14d-2(b))

|_|   Pre-commencement communications pursuant to Rule 13e-4(c) under the
      Exchange Act (17 CFR 240.13e-4(c))



Item 1.01.  Entry into a Material Definitive Agreement.

      On March 31,  2006,  Third  Wave  Technologies  Japan,  K.K.,  a  Japanese
corporation  ("Third  Wave  Japan") and  wholly-owned  subsidiary  of Third Wave
Technologies,  Inc. (the "Company"), entered into a Series A Preferred Stock and
Warrant   Purchase   Agreement  (the  "Purchase   Agreement")   with  Mitsubishi
Corporation ("Mitsubishi") and CSK Institute for Sustainability,  LTD., a wholly
owned  subsidiary  of CSK Holdings  ("CSK" and,  together with  Mitsubishi,  the
"Investors").  Under the Purchase Agreement,  Mitsubishi agreed to invest (Y)480
million  (approximately  $4.2 million) and CSK agreed to invest  (Y)100  million
(approximately $800,000) in Third Wave Japan in exchange for convertible, Series
A preferred  stock of Third Wave Japan and  warrants  to purchase an  additional
(Y)100 million (approximately  $800,000) worth of Third Wave Japan common stock.
Upon completion of the transactions  contemplated under the Purchase  Agreement,
the  Investors  will own  approximately  17% of Third  Wave  Japan  prior to the
exercise of the warrant or 20% after exercise of the warrant.  The  convertible,
Series A preferred  stock has an 8%  non-cumulative  dividend  payable as and if
declared  by  the  board,  a  liquidation  preference  over  the  common  stock,
redemption rights,  anti-dilution protection,  voting rights (on an as-converted
basis),  and veto  rights  regarding  key  matters  such as charter  amendments,
mergers,  and  liquidation.   The  Purchase  Agreement  is  subject  to  certain
conditions, and is expected to close before April 15, 2006.

      Upon closing of the Purchase  Agreement,  the Investors,  Third Wave Japan
and the Company will be parties to an Investor  Rights  Agreement (the "Investor
Rights  Agreement"),  pursuant to which the Investors  will have certain  rights
regarding Third Wave Japan including,  without  limitation,  (1) information and
inspection  rights,  (2) preemptive rights and co-sale rights,  (3) the right to
appoint 1 out of 4 Third Wave Japan  directors,  and (4) veto power over certain
matters including budget approval,  payment of dividends,  and certain licensing
and other  transactions  involving more than 10% of the fair market value of the
total  assets of Third Wave  Japan.  The  Investor  Rights  Agreement  will also
include  restrictions  on the  transfer  of Third Wave Japan  capital  stock and
provide  Third Wave Japan and its  shareholders  a right of first  refusal  with
respect to the sale of any Third Wave Japan  shares.  Upon a "change of control"
of the Company, the Company will have the right to purchase all Third Wave Japan
capital  stock held by the  Investors for the "fair value" of such stock and the
warrants will terminate.

      Upon closing of the Purchase  Agreement,  the Company and Third Wave Japan
will also be parties to an agreement  providing  for,  among other  things,  (1)
product  supply and  favorable  pricing for Third Wave Japan,  (2) an  exclusive
license for Third Wave Japan to utilize the Invader  technology in  Asia-Pacific
for human health applications, (3) an agreement that the Company will assign all
of its  Asia-Pacific-related  agreements to Third Wave Japan,  (4)  intellectual
property  ownership and  cross-licensing  provisions  relating to new inventions
made by Third Wave Japan, and (5) a commitment by the Company to provide certain
management  services,  and  technical  and customer  support,  in exchange for a
management fee of $500,000 per year for four years.

      A  copy  of  the  press  release  issued  by the  Company  announcing  the
transactions is attached as Exhibit 99.1.

Item 9.01. Financial Statements and Exhibits.

(d)   Exhibits.

      99.1  Press Release dated April 5, 2006.


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                                   SIGNATURES

      Pursuant to the  requirements of the Securities  Exchange Act of 1934, the
registrant  has fully  caused  this  report  to be  signed on its  behalf by the
undersigned, thereunto duly authorized.

                                  THIRD WAVE TECHNOLOGIES, INC.

Date: April 6, 2006               By: /s/ Kevin T. Conroy
                                      ------------------------------------------
                                      Name: Kevin T. Conroy
                                      Title: President & Chief Executive Officer



                                  EXHIBIT INDEX

Exhibit No.       Description
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     99.1         Press Release dated April 5, 2006.