Exhibit 25.1

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                       SECURITIES AND EXCHANGE COMMISSION
                             WASHINGTON, D.C. 20549
                           --------------------------

                                    FORM T-1

                         STATEMENT OF ELIGIBILITY UNDER
                      THE TRUST INDENTURE ACT OF 1939 OF A
                    CORPORATION DESIGNATED TO ACT AS TRUSTEE
               Check if an Application to Determine Eligibility of
                     a Trustee Pursuant to Section 305(b)(2)
             -------------------------------------------------------

                         U.S. BANK NATIONAL ASSOCIATION
              (Exact name of Trustee as specified in its charter)

                                   31-0841368
                       I.R.S. Employer Identification No.


           800 Nicollet Mall
         Minneapolis, Minnesota                           55402
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 (Address of principal executive offices)              (Zip Code)


                                  Frank Leslie
                         U.S. Bank National Association
                              60 Livingston Avenue
                               St. Paul, MN 55107
                                 (651) 495-3913
            (Name, address and telephone number of agent for service)


                                Diamond Jo, LLC.
                              Peninsula Gaming, LLC
                             Peninsula Gaming Corp.
                     (Issuer with respect to the Securities)


              Delaware                                     42-1483875
              Delaware                                     20-0800583
              Delaware                                     25-1902805
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    (State or other jurisdiction of         (I.R.S. Employer Identification No.)
     incorporation or organization)


     P.O. Box 90270
     Lafayette, Louisiana                                  70509-0270
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(Address of Principal Executive Offices)                   (Zip Code)


                       8.75% SENIOR SECURED NOTES DUE 2012
                       (TITLE OF THE INDENTURE SECURITIES)




                                    FORM T-1

ITEM 12.    GENERAL  INFORMATION.  Furnish the following  information  as to the
            Trustee.

            a)    Name and address of each examining or supervising authority to
                  which it is subject.

                          Comptroller of the Currency
                          Washington, D.C.


            b)    Whether it is authorized to exercise  corporate  trust powers.

                          Yes


ITEM 2.     AFFILIATIONS  WITH  OBLIGOR.  If the obligor is an  affiliate of the
            Trustee, describe each such affiliation.

                          None


ITEMS 3-15  Items 3-15 are not  applicable  because to the best of the Trustee's
            knowledge,  the obligor is not in default  under any  Indenture  for
            which the Trustee acts as Trustee.

ITEM 16.    LIST OF EXHIBITS:  List below all  exhibits  filed as a part of this
            statement of eligibility and qualification.

            1.    A copy of the Articles of Association of the Trustee.*

            2.    A copy of the  certificate  of  authority  of the  Trustee  to
                  commence business.*

            3.    A copy of the  certificate  of  authority  of the  Trustee  to
                  exercise corporate trust powers.*

            4.    A copy of the existing bylaws of the Trustee.*

            5.    A  copy  of  each  Indenture   referred  to  in  Item  4.  Not
                  applicable.

            6.    The consent of the Trustee  required by Section  321(b) of the
                  Trust Indenture Act of 1939, attached as Exhibit 6.

            7.    Report of  Condition  of the  Trustee  as of March  31,  2004,
                  published   pursuant  to  law  or  the   requirements  of  its
                  supervising or examining authority, attached as Exhibit 7.



     * Incorporated by reference to Registration Number 333-67188.


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                                      NOTE

      The  answers  to this  statement  insofar as such  answers  relate to what
persons have been  underwriters  for any securities of the obligors within three
years prior to the date of filing this statement,  or what persons are owners of
10% or more of the voting securities of the obligors,  or affiliates,  are based
upon information furnished to the Trustee by the obligors. While the Trustee has
no reason to doubt the accuracy of any such  information,  it cannot  accept any
responsibility therefor.



                                    SIGNATURE

      Pursuant  to the  requirements  of the  Trust  Indenture  Act of 1939,  as
amended,  the  Trustee,  U.S.  BANK  NATIONAL  ASSOCIATION,  a national  banking
association  organized  and  existing  under  the laws of the  United  States of
America,  has duly caused this statement of eligibility and  qualification to be
signed on its behalf by the undersigned,  thereunto duly authorized,  all in the
City of St. Paul, State of Minnesota on the 13th of July, 2004.


                                   U.S. BANK NATIONAL ASSOCIATION

                                   By:  /s/ Frank P. Leslie III
                                       ------------------------------------
                                       Frank P. Leslie III
                                       Vice President




By: /s/ Richard H. Prokosch
    ------------------------------
    Richard H. Prokosch
    Vice President





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                                    EXHIBIT 6

                                     CONSENT


      In accordance  with Section 321(b) of the Trust Indenture Act of 1939, the
undersigned,  U.S. BANK  NATIONAL  ASSOCIATION  hereby  consents that reports of
examination  of the  undersigned  by  Federal,  State,  Territorial  or District
authorities may be furnished by such  authorities to the Securities and Exchange
Commission upon its request therefor.


Dated:  July 13, 2004


                              U.S. BANK NATIONAL ASSOCIATION


                              By: /s/ Frank P. Leslie III
                                  ------------------------------------
                                  Frank P. Leslie III
                                  Vice President




By:  /s/ Richard H. Prokosch
    ------------------------------
    Richard H. Prokosch
    Vice President


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                                    EXHIBIT 7

                         U.S. BANK NATIONAL ASSOCIATION
                        STATEMENT OF FINANCIAL CONDITION
                                 AS OF 3/31/2004

                                    ($000'S)

                                                                       3/31/2004
                                                                       ---------
ASSETS
   Cash and Due From Depository Institutions                        $  7,180,778
   Federal Reserve Stock                                                       0
   Securities                                                         45,038,794
   Federal Funds                                                       2,593,702
   Loans & Lease Financing Receivables                               116,474,594
   Fixed Assets                                                        1,789,213
   Intangible Assets                                                  10,532,022
   Other Assets                                                        7,996,466
                                                                    ------------
      TOTAL ASSETS                                                  $191,605,569

LIABILITIES
   Deposits                                                         $126,605,087
   Fed Funds                                                           5,698,785
   Treasury Demand Notes                                               3,981,328
   Trading Liabilities                                                   252,912
   Other Borrowed Money                                               23,295,560
   Acceptances                                                           148,067
   Subordinated Notes and Debentures                                   5,807,310
   Other Liabilities                                                   5,587,914
                                                                    ------------
   TOTAL LIABILITIES                                                $171,376,963

EQUITY
   Minority Interest in Subsidiaries                                $  1,005,645
   Common and Preferred Stock                                             18,200
   Surplus                                                            11,677,397
   Undivided Profits                                                   7,527,364
                                                                    ------------
      TOTAL EQUITY CAPITAL                                          $ 20,228,606

TOTAL LIABILITIES AND EQUITY CAPITAL                                $191,605,569

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To the best of the undersigned's determination, as of the date hereof, the above
financial information is true and correct.

U.S. BANK NATIONAL ASSOCIATION

By: /s/ Frank P. Leslie III
    ----------------------------------
    Vice President

Date:  June 13, 2004


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