Exhibit 21 SUBSIDIARIES OF THE REGISTRANT The following is a list of all subsidiaries of Premier Financial Bancorp, Inc. and their state of incorporation. Subsidiary State of Incorporation ------------ ------------------------ Citizens Deposit Bank and Trust Company Kentucky Bank of Germantown Kentucky Georgetown Bancorp, Inc. Kentucky (Georgetown Bank and Trust Company) Citizens Bank Kentucky Premier Data Services, Inc. Kentucky Farmers Deposit Bancorp, Inc. Kentucky (Farmers Deposit Bank)