EXHIBIT 32.1 CERTIFICATION OF CHIEF EXECUTIVE OFFICER CALLISTO PHARMACEUTICALS, INC. FORM 10-K/A FOR THE YEAR ENDED DECEMBER 31, 2004 PURSUANT TO 18 U.S.C. SECTION 1350, AS ADOPTED PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002 I am the Chief Executive Officer of Callisto Pharmaceuticals, Inc., a Delaware corporation (the "Company"). I am delivering this certificate in connection with the Form 10-K/A of the Company for the year ended December 31, 2004 and filed with the Securities and Exchange Commission ("Form 10-K/A"). Pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, I hereby certify that, to the best of my knowledge, the Form 10-K/A fully complies with the requirements of Section 13(a) or Section 15(d) of the Securities Exchange Act of 1934 and that the information contained in the Form 10-K/A fairly presents, in all material respects, the financial condition and results of operations of the Company. Date: June 6, 2005 /s/ Gary S. Jacob ------------------------ Gary S. Jacob Chief Executive Officer