EXHIBIT 16.1 Madsen & Associates, CPA's Inc. 684 East Vine St., Suite 3 Murray, Utah 84107 September 16, 2005 Securities and Exchange Commission 450 5th Street, N.W. Washington, D.C. 20549 Ladies and Gentlemen: We have read and agree with the comments in Item 4.01 of Form 8-K Amendment No. 1 of Bionovo, Inc. (formerly Lighten Up Enterprises International, Inc.) dated May 25, 2005. /s/ Madsen & Associates, CPA's Inc. Madsen & Associates, CPA's Inc. Murray, Utah