EXHIBIT 23.1 CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM We hereby consent to the incorporation by reference in the Registration Statement on Form S-8 (File Nos. 333-108547, 333-98143, 333-90834, 333-67816, 333-44980 and 333-38151) of Cholestech Corporation of our report dated June 8, 2004 relating to the financial statements and financial statement schedule, which appears in this Form 10-K. /s/ PRICEWATERHOUSECOOPERS LLP San Jose, CA June 8, 2004