1 EXHIBIT 21 Greyhound Lines. Inc. Amarillo Trailways Bus Center Inc. (75%) Atlantic Greyhound Lines of Virginia, Inc. (100%) Continental Panhandle Lines, Inc. (50%) Gateway Ticketing Systems, Inc. (25%) GLI Holding Company (100%) ASI Associations, Inc. (100%) Carolina Associates, Inc. (100%) Carolina Coach Company (100%) Wilmington Union Bus Station Corporation (3%) Red Bus Systems, Inc. (100%) Seashore Transportation Company (100%) Wilmington Union Bus Station Corporation (39.5%) PRB Acquisition, LLC (99%) Texas New Mexico & Oklahoma Coaches, Inc. (100%) T.N.M.&O. Tours, Inc. (100%) Vermont Transit Co. Inc. (100%) Valley Garage Company (100%) Valley Transit Co., Inc. (100%) Greyhound de Mexico, S.A. de C.V. (99.9%) PRB Acquisition, LLC (1%) SET Acquisition Corp. (100%) Sistema Internacional De Transporte de Autobuses, Inc. (100%) American Bus Sales Associates, Inc. (100%) Americanos U.S.A., LLC (51%) Autobus Leasing Co., LLC (51%) Autobuses Americanos, S.A. de C. V. (49%) Autobuses Amigos, LLC (51%) Autobuses Amigos, S.A. de C. V. (49%) Autobuses Crucero, S.A. de C. V. (49%) Gonzalez, Inc. d/b/a Golden State Transportation (51%) Grupo Centro, Inc. (100%) Los Buenos Leasing Co., Inc. (100%) Los Rapidos, Inc. (51%) Omnibus Americanos, S.A. de C. V. (49%) Transportation Reality Income Partners, L.P. (48.9%) Union Bus Station of Oklahoma City, Oklahoma (40%) Wilmington Union Bus Station Corporation (24.6%)