. . . Exhibit 21.1 EXHIBIT 21.1 SUBSIDIARY LIST Laidlaw International, Inc. Delaware Laidlaw Transportation, Inc. Delaware Laidlaw Transit Holdings, Inc. Delaware Laidlaw Transit, Inc. Delaware Allied Bus Sales, Inc. Indiana Laidlaw Transit Management Company, Inc. Pennsylvania Laidlaw Transit Services, Inc. Delaware SuTran, Inc. South Dakota Van Tran of Tucson, Inc.(in process of dissolving) Arizona Safe Ride Services, Inc. Arizona Laidlaw Medical Holdings, Inc. Delaware Laidlaw Transportation Holdings, Inc. Delaware Greyhound Lines, Inc. Delaware [subsidiaries on separate page] Hotard Coaches, Inc. Louisiana Gray Line of New Orleans, Inc. (50%) Louisiana Mississippi Coast Limousine, Inc. Mississippi Chatham Coach Lines, Inc. Delaware Interstate Leasing, Inc. Mississippi Concorde Adjusters, Inc. Delaware Laidlaw Transportation Management, Inc. Ohio National Insurance and Indemnity Corporation Vermont Laidlaw USA, Inc. New York Laidlaw Two, Inc. Delaware Laidlaw International Finance Corporation (in process of dissolving) Ireland Laidlaw International Finance Corporation, Inc. Delaware American National Insurance Corporation Barbados First Transportation Indemnity Ltd. Barbados Greyhound Canada Transportation Corp. Ontario GCTC Leasing Ltd. Alberta Gray Coach Travel Inc. Ontario Greyhound Courier Express Ltd. British Columbia Penetang-Midland Coach Lines Limited Ontario 3765105 Canada Inc. Canada 2026922 Ontario Ltd. (will be amalgamated w/Laidlaw Transit, Ltd as of 8/31/06 Ontario Laidlaw Transit Ltd. Ontario Autobus Transco (1988) Inc. Quebec Capital Bus Sales(1988) Limited Ontario Manhattan Equipment Supply Company Limited Ontario Scott's Hospitality Limited England [GREYHOUND LINES,INC.] Atlantic Greyhound Lines of Virginia, Inc. Virginia Gateway Ticketing Systems, Inc. (25%) Pennsylvania Greyhound de Mexico, S.A. de C.V. (99.9%) Mexico LSX Delivery, L.L.C. Delaware RCL Liquidation, L.L.C (1%) Delaware Transportation Realty Income Partners L.P. (50%) Delaware Union bus Station of Oklahoma City, Oklahoma (40%) Oklahoma Wilmington Union Bus Station Corporation (55.1%) North Carolina GLI Holding Company Delaware Americanos U.S.A., L.L.C. (2%) Delaware Carolina Coach Company Virginia Wilmington Union Bus Station Corporation (3.4%) North Carolina Seashore Transportation Company North Carolina Wilmington Union Bus Station Corporation (39.1%) North Carolina GLI Corporate Risk Solutions, Inc. Delaware Greyhound Shore Services, L.L.C. Delaware Greyhound Transit Ltd. (20%) Cayman Islands On Time Delivery Service, Inc. Minnesota RCL Liquidation, L.L.C. (99%) Delaware Texas, New Mexico & Oklahoma Coaches, Inc. Delaware T.N.M. & O Tours, Inc. Texas Valley Garage Company Texas Valley Transit Co., Inc. Texas Vermont Transit Co., Inc. Vermont Sistema Internacional de Transporte de Autobuses, Inc. Delaware Americanos U.S.A., L.L.C. (98%) Delaware Autobuses Amigos, L.L.C. (51%) Delaware Crucero U.S.A., L.L.C. Delaware Giros Americanos, Inc. Delaware Gonzalez, Inc. d/b/a Golden State Transportation (51%) California Autobuses Amigos, S.A. de C.V. (49%) Republic of Mexico