EXHIBIT 25.1 ================================================================================ SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 ----------------------------- FORM T-1 STATEMENT OF ELIGIBILITY UNDER THE TRUST INDENTURE ACT OF 1939 OF A CORPORATION DESIGNATED TO ACT AS TRUSTEE ----------------------------- CHECK IF AN APPLICATION TO DETERMINE ELIGIBILITY OF A TRUSTEE PURSUANT - --- TO SECTION 305(b) (2) WELLS FARGO BANK, NATIONAL ASSOCIATION (Exact name of trustee as specified in its charter) A NATIONAL BANKING ASSOCIATION 94-1347393 (Jurisdiction of incorporation or (I.R.S. Employer organization if not a U.S. national Identification No.) bank) 101 NORTH PHILLIPS AVENUE SIOUX FALLS, SOUTH DAKOTA 57104 (Address of principal executive offices) (Zip code) WELLS FARGO & COMPANY LAW DEPARTMENT, TRUST SECTION MAC N9305-175 SIXTH STREET AND MARQUETTE AVENUE, 17TH FLOOR MINNEAPOLIS, MINNESOTA 55479 (612) 667-4608 (Name, address and telephone number of agent for service) ----------------------------- RENAL CARE GROUP, INC.(1) (Exact name of obligor as specified in its charter) DELAWARE 62-1622383 (State or other jurisdiction of (I.R.S. Employer incorporation or organization) Identification No.) 2525 WEST END AVENUE, SUITE 600 NASHVILLE, TENNESSEE 37203 (Address of principal executive offices) (Zip code) ----------------------------- 9.00% SENIOR SUBORDINATED NOTES DUE 2011 (Title of the indenture securities) ================================================================================ (1) See Table 1 - List of additional obligors, each of whose address of principal executive offices is 2525 West End Avenue, Suite 600, Nashville, Tennessee 37203. Table 1 The following subsidiaries of Renal Care Group, Inc. are guarantors of the exchange notes and are co-obligors: EXACT NAME OF ADDITIONAL OBLIGORS AS STATE OF INCORPORATION I.R.S. EMPLOYER SPECIFIED IN ITS CHARTER OR ORGANIZATION IDENTIFICATION NUMBER - ----------------------------------------------------- ------------------------- ---------------------- NNA of Oklahoma, Inc. Nevada 62-1842289 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Georgia, Inc. Delaware 62-1765493 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Alabama, Inc. Alabama 63-1223468 - ----------------------------------------------------- ------------------------- ---------------------- NNA Management Company of Kentucky, Inc. Kentucky 30-0005692 - ----------------------------------------------------- ------------------------- ---------------------- National Nephrology Associates Management Company Texas 74-2928108 of Texas, Inc. - ----------------------------------------------------- ------------------------- ---------------------- NNA of Nevada, Inc. Nevada 88-0435032 - ----------------------------------------------------- ------------------------- ---------------------- National Nephrology Associates Credit Corporation Tennessee 62-1838600 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Toledo, Inc. Ohio 34-1938916 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Rhode Island, Inc. Rhode Island 05-0513853 - ----------------------------------------------------- ------------------------- ---------------------- NNA Properties of New Jersey, Inc. New Jersey 68-0510777 - ----------------------------------------------------- ------------------------- ---------------------- NNA Management Company of Louisiana, Inc. Louisiana 62-1848873 - ----------------------------------------------------- ------------------------- ---------------------- Renex Corp. Florida 65-0422087 - ----------------------------------------------------- ------------------------- ---------------------- Renex Management Services, Inc. Florida 65-0837198 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Services of Atlanta, Inc. Georgia 65-0830360 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Penn Hills, Inc. Pennsylvania 25-1852225 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Shaler, Inc. Pennsylvania 25-1849434 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Doylestown, Inc. Pennsylvania 62-1851530 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Amesbury, Inc. Massachusetts 04-3257975 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of North Andover, Inc. Massachusetts 04-3333073 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of South Georgia, Inc. Georgia 58-2383123 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Creve Couer, Inc. Missouri 43-1690517 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of St. Louis, Inc. Missouri 43-1856441 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Bridgeton, Inc. Missouri 43-1717348 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Union, Inc. Missouri 43-1808427 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Homecare of Greater St. Louis, Inc. Missouri 43-1690516 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Maplewood, Inc. Missouri 43-1804718 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of University City, Inc. Missouri 43-1655681 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Facilities, Inc. Mississippi 64-0798295 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Bloomfield, Inc. New Jersey 22-3572775 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Orange, Inc. New Jersey 22-3461798 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Philadelphia, Inc. Pennsylvania 23-2812511 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Pittsburgh, Inc. Pennsylvania 25-1732278 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Woodbury, Inc. New Jersey 22-3461795 - ----------------------------------------------------- ------------------------- ---------------------- Renex Dialysis Clinic of Tampa, Inc. Florida 59-3244925 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Associates Medical Supply, LLC Tennessee 62-1735243 - ----------------------------------------------------- ------------------------- ---------------------- NNA-Saint Barnabas, L.L.C. New Jersey 14-1851729 - ----------------------------------------------------- ------------------------- ---------------------- NNA Saint Barnabas - Livingston, L.L.C. New Jersey 74-3070647 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Oklahoma, L.L.C. Oklahoma 73-1574780 ===================================================== ========================= ====================== ===================================================== ========================= ====================== NNA of Louisiana, LLC Louisiana 62-1848891 - ----------------------------------------------------- ------------------------- ---------------------- Doylestown Acute Renal Services, L.L.C. Pennsylvania 25-1863010 - ----------------------------------------------------- ------------------------- ---------------------- NNA of Newark, L.L.C. New Jersey 22-3757537 - ----------------------------------------------------- ------------------------- ---------------------- National Nephrology Associates of Texas, L.P. Texas 74-2928010 - ----------------------------------------------------- ------------------------- ---------------------- NNA Properties of Kentucky, Inc. Kentucky 68-0510773 - ----------------------------------------------------- ------------------------- ---------------------- NNA Properties of Tennessee, Inc. Tennessee 62-1869541 - ----------------------------------------------------- ------------------------- ---------------------- NNA Transportation Services Corporation Tennessee 62-1820401 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group East, Inc. Pennsylvania 23-1906900 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Michigan, Inc. Delaware 62-1738445 - ----------------------------------------------------- ------------------------- ---------------------- Michigan Home Dialysis Center, Inc. Michigan 38-2903593 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group of the Midwest, Inc. Kansas 48-0828924 - ----------------------------------------------------- ------------------------- ---------------------- Four State Regional Dialysis Center, Inc. Missouri 43-1331335 - ----------------------------------------------------- ------------------------- ---------------------- Fort Scott Regional Dialysis Center, Inc. Missouri 43-1474377 - ----------------------------------------------------- ------------------------- ---------------------- Miami Regional Dialysis Center, Inc. Missouri 43-1750093 - ----------------------------------------------------- ------------------------- ---------------------- RCG Mississippi, Inc. Delaware 62-1628022 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group of the Southeast, Inc. Florida 59-2851389 - ----------------------------------------------------- ------------------------- ---------------------- Northeast Alabama Kidney Clinic, Inc. Alabama 63-1181899 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Texas, Inc. Texas 75-1739434 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Management Corporation Texas 74-2596786 - ----------------------------------------------------- ------------------------- ---------------------- RCG PA Merger Corp. Texas 62-1710515 - ----------------------------------------------------- ------------------------- ---------------------- STAT Dialysis Corporation Delaware 74-2775421 - ----------------------------------------------------- ------------------------- ---------------------- Angleton Dialysis, Inc. Texas 76-0534299 - ----------------------------------------------------- ------------------------- ---------------------- Brazoria Kidney Center, Inc. Texas 76-0499032 - ----------------------------------------------------- ------------------------- ---------------------- Fondren Dialysis Clinic, Inc. Texas 76-0149309 - ----------------------------------------------------- ------------------------- ---------------------- Wharton Dialysis, Inc. Texas 76-0534300 - ----------------------------------------------------- ------------------------- ---------------------- Jefferson County Dialysis, Inc. Arkansas 71-0728066 - ----------------------------------------------------- ------------------------- ---------------------- KDCO, Inc. Missouri 43-1796126 - ----------------------------------------------------- ------------------------- ---------------------- Lawton Dialysis Inc. Arkansas 71-0719733 - ----------------------------------------------------- ------------------------- ---------------------- Little Rock Dialysis, Inc. Arkansas 71-0672705 - ----------------------------------------------------- ------------------------- ---------------------- Northwest Dialysis, Inc. Arkansas 72-1306514 - ----------------------------------------------------- ------------------------- ---------------------- RenaLab, Inc. Delaware 62-1694655 - ----------------------------------------------------- ------------------------- ---------------------- RCG Finance, Inc. Delaware 62-1710512 - ----------------------------------------------------- ------------------------- ---------------------- RenalPartners, Inc. Delaware 72-1365907 - ----------------------------------------------------- ------------------------- ---------------------- RenalNet, Inc. Delaware 72-1365902 - ----------------------------------------------------- ------------------------- ---------------------- Wound Care Group, Inc. Delaware 74-2775420 - ----------------------------------------------------- ------------------------- ---------------------- Diabetes Care Group, Inc. Delaware 62-1817411 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Arizona, Inc. Arizona 86-0441493 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Northwest, Inc. Delaware 93-1236337 - ----------------------------------------------------- ------------------------- ---------------------- RenalNet Arizona, Inc. Arizona 62-1757004 - ----------------------------------------------------- ------------------------- ---------------------- RCG University Division, Inc. Tennessee 62-1555316 - ----------------------------------------------------- ------------------------- ---------------------- R.C.G. Supply Company Tennessee 62-1564034 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Alaska, Inc. Alaska 92-0166049 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Southwest Holdings, Inc. Delaware 62-1757004 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Centers of America - Illinois, Inc. Illinois 37-1341578 - ----------------------------------------------------- ------------------------- ---------------------- SSKG, Inc. Illinois 36-4085833 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Ohio, Inc. Delaware 25-1789562 - ----------------------------------------------------- ------------------------- ---------------------- Physicians Dialysis Company, Inc. Pennsylvania 23-2858927 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Licensing Corp. Delaware 62-1864410 - ----------------------------------------------------- ------------------------- ---------------------- RCGIH, Inc. Delaware 62-1864412 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Southwest, L.P. Delaware 86-0960418 - ----------------------------------------------------- ------------------------- ---------------------- Arizona Renal Investments, LLC Delaware 86-1017618 - ----------------------------------------------------- ------------------------- ---------------------- RCG Indiana, L.L.C. Delaware 62-1674489 - ----------------------------------------------------- ------------------------- ---------------------- Kidney Disease Center of the Ozarks, L.L.C. Missouri 43-1734859 ===================================================== ========================= ====================== ===================================================== ========================= ====================== Stuttgart Dialysis, LLC Arkansas 71-0779483 - ----------------------------------------------------- ------------------------- ---------------------- RCG West Health Supply, L.C. Arizona 86-0738042 - ----------------------------------------------------- ------------------------- ---------------------- Kentucky Renal Care Group, LLC Delaware 36-4494425 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Texas, LP Delaware 84-1620338 - ----------------------------------------------------- ------------------------- ---------------------- Renal Care Group Westlake, LLC Delaware 65-1185177 - ----------------------------------------------------- ------------------------- ---------------------- Dialysis Associates, LLC Tennessee 62-1727146 ===================================================== ========================= ====================== Item 1. General Information. Furnish the following information as to the trustee: (a) Name and address of each examining or supervising authority to which it is subject. Comptroller of the Currency Treasury Department Washington, D.C. Federal Deposit Insurance Corporation Washington, D.C. Federal Reserve Bank of San Francisco San Francisco, California 94120 (b) Whether it is authorized to exercise corporate trust powers. The trustee is authorized to exercise corporate trust powers. Item 2. Affiliations with Obligor. If the obligor is an affiliate of the trustee, describe each such affiliation. None with respect to the trustee. No responses are included for Items 3-14 of this Form T-1 because the obligor is not in default as provided under Item 13. Item 15. Foreign Trustee. Not applicable. Item 16. List of Exhibits. List below all exhibits filed as a part of this Statement of Eligibility. Exhibit 1. A copy of the Articles of Association of the trustee now in effect.* Exhibit 2. A copy of the Comptroller of the Currency Certificate of Corporate Existence and Fiduciary Powers for Wells Fargo Bank, National Association, dated February 4, 2004.** Exhibit 3. See Exhibit 2 Exhibit 4. Copy of By-laws of the trustee as now in effect.*** Exhibit 5. Not applicable. Exhibit 6. The consent of the trustee required by Section 321(b) of the Act. Exhibit 7. A copy of the latest report of condition of the trustee published pursuant to law or the requirements of its supervising or examining authority. Exhibit 8. Not applicable. Exhibit 9. Not applicable. * Incorporated by reference to the exhibit of the same number to the trustee's Form T-1 filed as exhibit 25 to the Form T-3 dated March 3, 2004 of Trans-Lux Corporation file number 022-28721. ** Incorporated by reference to the exhibit of the same number to the trustee's Form T-1 filed as exhibit 25 to the Form T-3 dated March 3, 2004 of Trans-Lux Corporation file number 022-28721. *** Incorporated by reference to the exhibit of the same number to the trustee's Form T-1 filed as exhibit 25 to the Form T-3 dated March 3, 2004 of Trans-Lux Corporation file number 022-28721. SIGNATURE Pursuant to the requirements of the Trust Indenture Act of 1939, as amended, the trustee, Wells Fargo Bank, National Association, a national banking association organized and existing under the laws of the United States of America, has duly caused this statement of eligibility to be signed on its behalf by the undersigned, thereunto duly authorized, all in the City of Minneapolis and State of Minnesota on the 13th day of December 2004. WELLS FARGO BANK, NATIONAL ASSOCIATION /s/ Timothy P. Mowdy --------------------------- Timothy P. Mowdy Assistant Vice President EXHIBIT 6 December 13, 2004 Securities and Exchange Commission Washington, D.C. 20549 Gentlemen: In accordance with Section 321(b) of the Trust Indenture Act of 1939, as amended, the undersigned hereby consents that reports of examination of the undersigned made by Federal, State, Territorial, or District authorities authorized to make such examination may be furnished by such authorities to the Securities and Exchange Commission upon its request therefor. Very truly yours, WELLS FARGO BANK, NATIONAL ASSOCIATION /s/ Timothy P. Mowdy --------------------------- Timothy P. Mowdy Assistant Vice President EXHIBIT 7 Consolidated Report of Condition of Wells Fargo Bank National Association of 101 North Phillips Avenue, Sioux Falls, SD 57104 And Foreign and Domestic Subsidiaries, at the close of business September 30, 2004, filed in accordance with 12 U.S.C. Section 161 for National Banks. Dollar Amounts In Millions -------------- ASSETS Cash and balances due from depository institutions: Noninterest-bearing balances and currency and coin $ 13,183 Interest-bearing balances 2,782 Securities: Held-to-maturity securities 0 Available-for-sale securities 30,191 Federal funds sold and securities purchased under agreements to resell: Federal funds sold in domestic offices 5,017 Securities purchased under agreements to resell 961 Loans and lease financing receivables: Loans and leases held for sale 33,062 Loans and leases, net of unearned income 240,775 LESS: Allowance for loan and lease losses 2,467 Loans and leases, net of unearned income and allowance 238,308 Trading Assets 5,989 Premises and fixed assets (including capitalized leases) 3,273 Other real estate owned 122 Investments in unconsolidated subsidiaries and associated companies 299 Customers' liability to this bank on acceptances outstanding 112 Intangible assets Goodwill 8,558 Other intangible assets 8,485 Other assets 12,631 -------- Total assets $362,973 ======== LIABILITIES Deposits: In domestic offices $261,252 Noninterest-bearing 79,485 Interest-bearing 181,767 In foreign offices, Edge and Agreement subsidiaries, and IBFs 18,543 Noninterest-bearing 3 Interest-bearing 18,540 Federal funds purchased and securities sold under agreements to repurchase: Federal funds purchased in domestic offices 11,909 Securities sold under agreements to repurchase 3,155 Dollar Amounts In Millions -------------- Trading liabilities 4,285 Other borrowed money (includes mortgage indebtedness and obligations under capitalized leases) 15,091 Bank's liability on acceptances executed and outstanding 112 Subordinated notes and debentures 4,531 Other liabilities 10,005 -------- Total liabilities $328,883 Minority interest in consolidated subsidiaries 53 EQUITY CAPITAL Perpetual preferred stock and related surplus 0 Common stock 520 Surplus (exclude all surplus related to preferred stock) 24,512 Retained earnings 8,305 Accumulated other comprehensive income 700 Other equity capital components 0 -------- Total equity capital 34,037 -------- Total liabilities, minority interest, and equity capital $362,973 ======== I, James E. Hanson, Vice President of the above-named bank do hereby declare that this Report of Condition has been prepared in conformance with the instructions issued by the appropriate Federal regulatory authority and is true to the best of my knowledge and belief. James E. Hanson Vice President We, the undersigned directors, attest to the correctness of this Report of Condition and declare that it has been examined by us and to the best of our knowledge and belief has been prepared in conformance with the instructions issued by the appropriate Federal regulatory authority and is true and correct. Dave Hoyt Howard Atkins Directors Pat Callahan