As filed with the Securities and Exchange Commission on December 10, 1998.
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                                                  Registration No. 333-64563    
     

                       SECURITIES AND EXCHANGE COMMISSION
                              Washington D.C. 20549

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                                  PRE-EFFECTIVE
                               AMENDMENT NO. 2 TO
                                    FORM S-3
             REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933    
     
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                               BIO-VASCULAR, INC.
             (Exact name of registrant as specified in its charter)
       
                Minnesota                               41-1526554
       (State or other jurisdiction         (I.R.S. Employer Identification No.)
      of incorporation or organization)           

                                  -------------
                             2575 University Avenue
                            St. Paul, Minnesota 55114
                                 (651) 603-3700
               (Address, including zip code, and telephone number,
        including area code, of registrant's principal executive offices)

                                   ----------

                               Connie L. Magnuson
              Vice President - Finance and Chief Financial Officer
                             2575 University Avenue
                            St. Paul, Minnesota 55114
                                 (651) 603-3700
            (Name, address, including zip code, and telephone number,
                   including area code, of agent for service)

                                 ---------------

                                   Copies to:
                             Michael J. Kolar, Esq.
                        Oppenheimer Wolff & Donnelly LLP
                     3400 Plaza VII, 45 South Seventh Street
                          Minneapolis, Minnesota 55402
                                 (612) 607-7000

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        Approximate date of commencement of proposed sale to the public:
   From time to time after the effective date of this Registration Statement.

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     If the only securities being registered on this Form are being offered
pursuant to dividend or interest reinvestment plans, please check the following
box. [ ]

     If any of the securities being registered on this Form are to be offered on
a delayed or continuous basis pursuant to Rule 415 under the Securities Act of
1933, other than securities offered only in connection with dividend or interest
reinvestment plans, check the following box. [x]

     If this Form is filed to register additional securities for an offering
pursuant to Rule 462(b) under the Securities Act, please check the following box
and list the Securities Act registration statement number of the earlier
effective registration statement for the same offering. [ ]

     If this Form is a post-effective amendment filed pursuant to Rule 462(c)
under the Securities Act, check the following box and list the Securities Act
registration statement number of the earlier effective registration statement
for the same offering. [ ]

     If delivery of the prospectus is expected to be made pursuant to Rule 434,
please check the following box. [ ]

       
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     THE REGISTRANT HEREBY AMENDS THIS REGISTRATION STATEMENT ON SUCH DATE OR
DATES AS MAY BE NECESSARY TO DELAY ITS EFFECTIVE DATE UNTIL THE REGISTRANT SHALL
FILE A FURTHER AMENDMENT WHICH SPECIFICALLY STATES THAT THIS REGISTRATION
STATEMENT SHALL THEREAFTER BECOME EFFECTIVE IN ACCORDANCE WITH SECTION 8(A) OF
THE SECURITIES ACT OF 1933 OR UNTIL THE REGISTRATION STATEMENT SHALL BECOME
EFFECTIVE ON SUCH DATE AS THE COMMISSION, ACTING PURSUANT TO SAID SECTION 8(A),
MAY DETERMINE.

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                                     PART II
                     INFORMATION NOT REQUIRED IN PROSPECTUS
       

Item 16. Exhibits

2.1  Acquisition Agreement and Plan of Reorganization by and among Bio-Vascular,
     Inc., Jer-Neen Acquisition, Inc., Jer-Neen Manufacturing Co., Inc., George
     Nelson, Jr., Ronald Breckner, James Pfau, Willard Sykes and Catherine
     Sykes, dated July 31, 1998 (incorporated by reference to Exhibit 2.1 to the
     Company's Current Report on Form 8-K, dated July 31, 1998, as amended (File
     No. 0-13907)).

4.1  Restated Articles of Incorporation of the Company, as amended,
     (incorporated by reference to Exhibit 3.1 to the Company's Quarterly Report
     on Form 10-Q for the quarter ended April 30, 1997 (File No. 0-13907)).

4.2  Amended and Restated Bylaws of the Company (incorporated by reference to
     Exhibit 3.2 to the Company's Registration Statement on Form S-4 (File No.
     33-74750)).

4.3  Form of Common Stock Certificate of the Company (incorporated by reference
     to Exhibit 4.1 to the Company's Registration Statement on Form 10 (File
     0-13907)).

4.4  Rights Agreement, dated as of June 12, 1996, between Bio-Vascular, Inc. and
     American Stock Transfer & Trust Company, which includes as Exhibit A the
     form of Rights Certificate (incorporated by reference to Exhibit 4.1 to the
     Company's Current Report on Form 8-K dated June 12, 1996 (File No.
     0-13907)).

5.1  Opinion and Consent of Oppenheimer Wolff & Donnelly LLP (filed herewith
     electronically).

    
23.1 Consent of PricewaterhouseCoopers LLP (filed herewith electronically).*
     

23.2 Consent of Oppenheimer Wolff & Donnelly LLP (included in Exhibit 5.1).

   
24.1 Power of Attorney (included on page II-4 of this Registration Statement).*
    


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*Indicates item previously filed    

 
                                   SIGNATURES

   
   
     Pursuant to the requirements of the Securities Act of 1933, the registrant
certifies that it has reasonable grounds to believe that it meets all of the
requirements for filing on Form S-3 and has duly caused this Registration
Statement to be signed on its behalf by the undersigned, thereunto duly
authorized, in the City of St. Paul and State of Minnesota, on December 10,
1998.    
    


                           By: /s/ Connie L. Magnuson
                               --------------------------------
                               Connie L. Magnuson
                               Vice President - Finance and Chief Financial
                               Officer (Principal Financial and Accounting
                               Officer)


   
   
     Pursuant to the requirements of the Securities Act of 1933, this
Registration Statement has been signed by the following persons on December 10,
1998 in the capacities indicated.
    
    
   
     Signature                                    Title
     ---------                                    -----
*                                     President, Chief Executive Officer and 
- ----------------------------          Director (Principal Executive Officer)

M. Karen Gilles

*                                     Chairman of the Board and Director
- ----------------------------
Timothy M. Scanlan

*                                     Director
- ----------------------------
William G. Kobi

*                                     Director
- ----------------------------
Richard W. Perkins

*                                     Director
- ----------------------------
Anton R. Potami

*                                     Director
- ----------------------------
Timothy M. Scanlan

*                                     Director
- ----------------------------
Edward E. Strickland
    

   
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* By: /s/ Connie L. Magnuson
      ---------------------------
      Connie L. Magnuson
      Attorney-in-fact    



                                      II-2

 
   
                               BIO-VASCULAR, INC.    
               EXHIBIT INDEX TO REGISTRATION STATEMENT ON FORM S-3



Item No.    Description                                   Method of Filing
- --------    -----------                                   ----------------
                                                          
2.1         Acquisition  Agreement  and Plan of 
            Reorganization  by and among  
            Bio-Vascular,  Inc.,  Jer-Neen 
            Acquisition,  Inc., Jer-Neen 
            Manufacturing Co., Inc., George Nelson, Jr., 
            Ronald Breckner,  James Pfau, Willard 
            Sykes and Catherine Sykes, dated 
            July 31, 1998.................................Incorporated by 
                                                          reference to Exhibit 
                                                          2.1 to the Company's 
                                                          Current Report on Form 
                                                          8-K, dated July 31, 
                                                          1998, as amended (File 
                                                          No. 0-13907).

4.1         Restated Articles of Incorporation of the 
            Company, as amended...........................Incorporated by 
                                                          reference to Exhibit 
                                                          3.1 to the Company's 
                                                          Quarterly Report on 
                                                          Form 10-Q for the 
                                                          quarter ended April 
                                                          30, 1997 (File No. 
                                                          0-13907).

4.2         Amended and Restated Bylaws of the            
            Company.......................................Incorporated by 
                                                          reference to Exhibit
                                                          3.2 to the Company's
                                                          Registration Statement
                                                          on Form S-4 (File
                                                          No. 33-74750).

4.3         Form of Common Stock Certificate of the 
            Company.......................................Incorporated by 
                                                          reference to 
                                                          Exhibit 4.1 to the 
                                                          Company's Registration
                                                          Statement on Form 10 
                                                          (File 0-13907).

4.4         Rights Agreement,  dated as of June 12, 1996,
            between Bio-Vascular,  Inc. and American 
            Stock Transfer & Trust Company, which 
            includes as Exhibit A the form of Rights 
            Certificate...................................Incorporated by 
                                                          reference to Exhibit 
                                                          4.1 to the Company's 
                                                          Current Report on Form
                                                          8-K dated June 12, 
                                                          1996 (File No. 
                                                          0-13907)).

5.1         Opinion and Consent of Oppenheimer Wolff & 
            Donnelly LLP..................................Filed herewith 
                                                          electronically.

   
23.1        Consent of PricewaterhouseCoopers LLP.........Filed herewith 
                                                          electronically.*
    

23.2        Consent of Oppenheimer Wolff & Donnelly LLP...Included in 
                                                          Exhibit 5.1.

   
24.1        Power of Attorney.............................Included on page II-4 
                                                          of this Registration 
                                                          Statement.*    


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*Indicates item previously filed