<Page>

EXHIBIT 3.27

AMENDED AND RESTATED
ARTICLES OF INCORPORATION

PROFIT

The undersigned corporation, pursuant to Section 79-4-10.06 and
79-4-10.07 of the Mississippi Code of 1972, hereby execute the following
document and sets forth:

A. The name of the corporation is: Sturgis, Samson & Henderson Pathology
Laboratory, P.A.

The name of the corporation is changed to: Sturgis, Henderson &
Proctor Pathology Laboratory, P.A.

B. The text of the Restated Articles of Incorporation is as follows:

1. The period of its duration is perpetual.

2. The specific purpose or purposes for which the corporation is
[SEAL] organized, stated in general terms, are: to engage in the
practice of medicine, specifically in anatomic and clinical
pathology; to operate laboratories and all types of equipment
used therein or incidental thereto; to do anything necessary,
suitable or proper to the attainment of any of the purposes as
authorized by the Mississippi Professional Corporation Act and
the Mississippi Business Corporation Act.

3. The aggregate number of shares which the corporation shall have
authority to issue is 2,500 with a par value of Ten Dollars
($10.00) each.

4. The corporation shall not commence business until consideration
of the value of at least One Thousand Dollars ($1,000.00) has
been received for the issuance of shares.

5. Provisions granting to shareholders the preemptive right to
acquire additional or treasury shares of the corporation are:
each shareholder shall, upon the sale of any issued or treasury
shares of the corporation, or obligations of the corporation
convertible into such shares, have the right to purchase, for
cash, hit pro rata share thereof at the price at which said
shares or obligations are offered to others.

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6. Domicile address: 1033 North Flowood Drive, Jackson, Mississippi
39208.

7. The corporation's registered agent is George M. Sturgis, M.D. The
registered office is 1033 North Flowood Drive, Jackson,
Mississippi 39208.

8. The initial board of directors were:

George M. Sturgis, M.D.
440 East Woodrow Wilson
Jackson, Mississippi

Roland F. Samson, M.D.
440 East Woodrow Wilson
Jackson, Mississippi

Julian C. Henderson, M.D,
440 East Woodrow Wilson
Jackson, Mississippi

9. The name and address of each incorporator was:

George M. Sturgis, M.D.
440 East Woodrow Wilson
Jackson, Mississippi

Roland F. Samson, M.D.
440 East Woodrow Wilson
Jackson, Mississippi

Julian C. Henderson, M.D.
440 East Woodrow Wilson
Jackson, Mississippi

10. All duties, responsibilities, rights, authority and obligations
imposed on and enjoyed by the Board of Directors of a corporation
shall be imposed on and enjoyed by the shareholders of this
corporation.

C. The amendment and restatement were adopted on the 1st day of July
1992, by the shareholders.

2
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D. 1. The designation, number of outstanding shares, number of votes
entitled to be cast by each voting group entitled to vote
separately on the restatement, and the number of votes of each
voting group indisputably represented at the meeting was:

<Table>
<Caption>
NUMBER OF NUMBER OF NO OF VOTES
DESIGNATION OUTSTANDING VOTES ENTITLED INDISPUTABLY
OF SHARES SHARES TO BE CAST REPRESENTED
----------- ----------- -------------- -------------

Common Stock 1,000 1,000 1,000
</Table>

2. The total number of votes cast for and against the amendment by
each voting group entitled to vote separately on the amendment
was:

<Table>
<Caption>
TOTAL NO. OF TOTAL NO OF VOTES
VOTING GROUP VOTES CAST FOR CAST AGAINST
------------ -------------- -----------------

Common Stock 1000 -0-
</Table>

STURGIS, HENDERSON & PROCTOR
PATHOLOGY LABORATORY, P.A.

BY: /s/ Julian C Henderson;
---------------------------
Its: Secretary
--------------------------
3
<Page>

Young, Scanlon and Sessums, P.A.
Attorneys at Law

August 21, 1992

Sandra C. Bradshaw
Bobby L. Dallas
Sean Wesley Ellis
Stephen E. Gardner
Paul B. Henderson

James H. Neeld, IV
Gee Ogletree
Mike Parker
Gretchen Pumphrey
Pat H. Scanlon
Brad Sessums
John S. Simpson, Jr.
John S. Simpson
Robert L. Wells
E. Stephen Williams
James Leon Young
J. Will Young,
Of Counsel

- ------------
Tony Carlisle,
Administrator

HAND DELIVERY

Ms. Deb Price
Corporation Division
Secretary of State
Magnolia Bank Building
Jackson, Mississippi

RE: Sturgis, Samson & Henderson Pathology Laboratory, P.A.

Dear Deb:

Enclosed are duplicate originals of the Amended and Restated Articles of
Incorporation for the above-referenced company.

Also enclosed are our firm checks totaling $75 to cover filing fees and
non-standard form fee.

Thank you for your help.

Sincerely.

/s/ Kathleen F. Haynes

Kathleen F. Haynes
Legal Assistant

KFH:bcd
Enclosures

2000 Deposit Guaranty Plaza - P.O. Box 23059 Jackson, Mississippi 39225-3059
Telephone: (601) 948-6100 FAX (601) 355-6136

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FOO12 - Page 1 of 3 OFFICE OF THE MISSISSIPPI SECRETARY OF STATE
P.O. BOX 136, JACKSON, MS 39205-0136 (601)359-1333
Articles of Amendment

The undersigned persons, pursuant to Section 79-4-10.06 (if a profit
corporation) or Section 79-11-305 (if a nonprofit corporation) of the
Mississippi Code of 1972, hereby execute the following document and set forth:

1. TYPE OF CORPORATION

/X/ Profit / / Nonprofit

2. NAME OF CORPORATION

Sturgis, Henderson & Proctor Pathology Laboratory, PA.

3. THE FUTURE EFFECTIVE DATE IS AUGUST 29, 1997
(Complete if applicable)

[SEAL]

4. SET FORTH THE TEXT OF EACH AMENDMENT ADOPTED. (ATTACH PAGE)

5. IF AN AMENDMENT FOR A BUSINESS CORPORATION PROVIDES FOR AN EXCHANGE,
RECLASSIFICATION, OR CANCELLATION OF ISSUED SHARES, SET FORTH THE PROVISIONS FOR
IMPLEMENTING THE AMENDMENT IF THEY ARE NOT CONTAINED IN THE AMENDMENT ITSELF.
(ATTACH PAGE)

6. THE AMENDMENT(s) WAS (WERE) ADOPTED ON

August 27, 1997 Date(s)

FOR PROFIT CORPORATION (Check the appropriate box)

Adopted by / / the incorporators / / directors without shareholder action
and shareholder action was not
required.

FOR NONPROFIT CORPORATION (Check the appropriate box)

Adopted by / / the incorporators / / board of directors without member
action and member action was not
required.

FOR PROFIT CORPORATION

7. IF THE AMENDMENT WAS APPROVED BY SHAREHOLDERS
(a) The designation, number of outstanding shares, number of votes entitled to
be cast by each voting group entitled to vote separately on the amendment, and
the number of votes of each voting group indisputably represented at the meeting
were

<Table>
<Caption>
Designation No. of outstanding No. of votes entitled No. of Votes
Shares to be case indisputably represented

Common 1,000 1,000 1,000
</Table>

Rev. 06/94

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FOO12 - Page 2 of 3 OFFICE OF THE MISSISSIPPI SECRETARY OF STATE
P.O. BOX 136, JACKSON, MS 39205-0136 (601)359-1333
Articles of Amendment

<Table>

</Table>

(b) EITHER

(i) the total number of votes cast for and against the amendment by each
voting group entitled to vote separately on the amendment was

<Table>
<Caption>
Total no. of Total no. of votes
Voting group votes cast FOR cast AGAINST
- ------------ -------------- ------------------

Common Stock 1,000 0
</Table>

(ii) the total number of undistributed votes cast for the amendment by each
voting group was

<Table>
<Caption>
Voting group Total no. of undisputed votes cast FOR the plan
- ------------ -----------------------------------------------

</Table>

and the number of votes cast for the amendment by each voting group was
sufficient or approval by that voting group.

FOR NONPROFIT CORPORATION

8. IF THE AMENDMENT WAS APPROVED BY THE MEMBERS
(a) The designation, number of memberships outstanding, number of votes entitled
to be cast by each class entitled to vote separately on the amendment, and the
number of votes of each class indisputably represented at the meeting were

<Table>
<Caption>
Designation No. of memberships No. of votes entitled No. of votes
outstanding to be cast indisputably represented

</Table>

Rev. 06/94

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FOO12 - Page 3 of 3 OFFICE OF THE MISSISSIPPI SECRETARY OF STATE
P.O. BOX 136, JACKSON, MS 39205-0136 (601)359-1333
Articles of Amendment

(b) EITHER

(i) the total number of votes cast for and against the amendment by each
class entitled to vote separately on the amendment was

<Table>
<Caption>
Total no. of Total no. of votes
Voting class votes cast FOR cast AGAINST
- ------------ -------------- ------------------

</Table>

<Table>
<Caption>
Total no. of undisputed votes cast FOR the
Voting class amendment
- ------------ -----------------------------------------------

</Table>

and the number of votes cast for the amendment by each voting group was
sufficient for approval by that voting group.

By: Signature /s/ George M. Sturgis (please keep writing within blocks)

Printed Name George M. Sturgis, M.D. Title President

Rev. 06/94

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ATTACHMENT TO ARTICLES OF AMENDMENT OF

STURGIS, HENDERSON & PROCTOR PATHOLOGY LABORATORY, P.A.

Article A of the amended and restated of Articles of Incorporation of
Sturgis Henderson & Proctor Pathology Laboratory, P.A. is hereby amended to read
as follows:

A. The name of the corporation is: AmeriPath Mississippi, Inc.

Paragraph 2 of Article B of the amended and restated Articles of
Incorporation of Sturgis Henderson & Proctor Pathology Laboratory, P.A. is
hereby amended to read as follows:

2. The specific purpose or purposes for which the corporation is
organized, stated in general terms are: to engage in any lawful
purpose authorized by the Mississippi Business Corporation Act.

Paragraph 5 of Article B is amended to delete that paragraph in its
entirety.

Article B is amended to renumber the previous paragraphs of Article B which
were Paragraphs 6 through 10 as Paragraphs 5 through 9, respectively.

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YOUNG, WILLIAMS, HENDERSON & FUSELIER, PA

Attorneys at Law

August 27, 1997

HAND DELIVERED

Secretary of State
Attention: Deb Price
Corporate Division

202 North Congress Street, Suite 601
Jackson, Mississippi 39201

Re: Sturgis, Henderson & Proctor Pathology Laboratory, P.A.
Dear Deb:

Enclosed are duplicate originals of Articles of Amendment for Sturgis,
Henderson & Proctor Pathology Laboratory, P.A., along with a check for $50.00
which represents the filing fee. I would appreciate your filing this document
as quickly as possible. Please give me a call when it is ready to be picked up
and I will send a runner.

Thank you for your assistance and cooperation.

Sincerely,

/s/ Don H. Goode

Don H. Goode

DHG:cmg
Enclosures

[YOUNG, WILLIAMS, HENDERSON & FUSELIER, PA LOGO]

J. Wesley Daughdrill, Jr.
Sean Wesley Ellis
Louis A. Puselier
Stephen E. Gardner
Don H. Goode
Chad Hammons
Philip C, Hearn
Paul B. Henderson
E. B. Martin, Jr.
Tammy D, Middleton
Lisa Binder Milner
James H. Neeld, IV
Robert L. Wells
E. Stephen Williams
James Leon Young

J. Will Young.
(1906-1996)

Tony Carlisle,
Administrator

2000 Deposit Guaranty Plaza
Jackson, Missisippi 39201

Post Office Box 23059
Jackson, Mississippi 39225-306

(601) 948-6100
Fax (601) 355-6136