<Page> EXHIBIT 23.1 CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM We hereby consent to the use in this Amendment No. 1 to Registration Statement No. 333-124312 of Patriot National Bancorp, Inc. on Form SB-2 of our report, dated March 3, 2005, appearing in the Prospectus, which is a part of this Registration Statement. We also consent to the reference to our firm under the caption "Experts" in such Prospectus. /s/ McGladrey & Pullen, LLP New Haven, Connecticut June 20, 2005