Exhibit 16.1 ACM Anton Collins Mitchell LLP February 22, 2005 Securities and Exchange Commission 450 Fifth Street, N.W. Washington, D.C. 20549 Ladies and Gentlemen: We have read the first five paragraphs of Item 4 included in the Form 8-K of Accelr8 Technology Corporation dated February 18, 2005 to be filed with the Securities and Exchange Commission and are in agreement with the statements contained therein. Very truly yours, /s/ Anton Collins Mitchell LLP Anton Collins Mitchell LLP Accountants & Consultants 17th & Grant Building. 303 East 17th Avenue, Suite 600 . Denver, Colorado 80203 303.830.1120 . Fax 303 830 8130