Exhibit 5.1

                             TROY J. RILLO, ESQUIRE
                                 2574 JARDIN WAY
                              WESTON, FLORIDA 33327

                                   May 6, 2004

Bio-One Corporation
1630 Winter Springs Boulevard
Winter Springs, Florida 32708

         Re:      Bio-One Corporation
                  Registration Statement on Form SB-2

Gentlemen:

         I have acted as special counsel to Bio-One Corporation (the "Company")
in connection with the issuance or proposed issuance of 293,384,616 shares of
the Company's common stock (the "Common Stock"). The resale of the Common stock
is being registered on a Registration Statement on Form SB-2 (the "Registration
Statement")

         You have requested my opinion as to the matters set forth below in
connection with the issuance or proposed issuance of the Common Stock. For
purposes of rendering that opinion, I am familiar with the Registration
Statement, and I have examined the Company's Articles of Incorporation, as
amended to date, the Company's Bylaws, as amended to date, and corporate action
of the Company that provides for the issuance of the Common Stock. I have also
examined such other documents, certificates, instruments and corporate records,
and such statutes, decisions and questions of law as I have deemed necessary or
appropriate for the purpose of this opinion. I have examined and relied upon
certificates of public officials and, as to certain matters of fact that are
material to our opinion, I have also relied on a certificate of an officer of
the Company. In rendering my opinion, I also have made the assumptions that are
customary in opinion letters of this kind. I have not verified any of those
assumptions.

         My opinion set forth below is limited to Nevada Revised Statutes,
Chapter 78, including the applicable provisions of the Nevada Constitution and
reported judicial decisions interpreting those laws.

         Based upon and subject to the foregoing, it is my opinion that the
shares of Common Stock to be sold pursuant to the Registration Statement by the
selling shareholders named therein, were and are duly authorized for issuance by
the Company and, when issued and sold in the manner described in the
Registration Statement, as amended, will be validly issued, fully paid and
non-assessable.

         I hereby consent to the filing of this opinion as an Exhibit to the
Registration Statement and to the use of my name in the Prospectus constituting
a part thereof. In giving my consent I do not thereby admit that I am in the
category of persons whose consent is required under Section 7 of the Securities
Act of 1933 or the rules and regulations thereunder.

                                                     Very truly yours,

                                                     /s/ Troy J. Rillo

                                                     Troy J. Rillo