Exhibit 32.1

CERTIFICATE OF THE PRINCIPAL EXECUTIVE OFFICER
OF DIOMED HOLDINGS, INC. (REGISTRANT)
PURSUANT TO SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002

         I, James A. Wylie,  Jr.,  Chief  Executive  Officer of the  Registrant,
certify to the best of my  knowledge  and  belief  pursuant  to  Section  906 of
Sarbanes-Oxley Act of 2002 (18 U.S.C. Section 1350) that:

          (1)     The  Quarterly  Report on Form  10-QSB  for the  period  ended
                  September 30, 2003,  which this statement  accompanies,  fully
                  complies  with the  requirements  of section 13(a) or 15(d) of
                  the Securities Exchange Act of 1934; and

          (2)     The  information  contained  in the  Quarterly  Report on Form
                  10-QSB  fairly  presents,   in  all  material  respects,   the
                  financial   condition   and  result  of   operations   of  the
                  Registrant.


Date:  November  7, 2003                /s/ JAMES A. WYLIE, JR.
                                        ---------------------------
                                        James A. Wylie, Jr.
                                        Chief Executive Officer
                                        (Principal Executive Officer)