Pharma-Bio Serv, Inc.
                        Sardinera Beach Building Suite 2
                              Marginal Costa de Oro
                            Dorado, Puerto Rico 00646

                                 October 4, 2006

Securities and Exchange Commission
Washington, DC 20549
Attention:   Karen J. Garnett, Esq.
             Assistant Director

                        Re:   Pharma-Bio Serv, Inc.
                              Registration Statement on Form SB-2
                              File No. 333-132847
                              -----------------------------------

Ladies and Gentlemen:

      Pharma-Bio Serv, Inc. (the "Company"), is filing amendment No. 4 to the
captioned registration statement on Form SB-2 in response to the questions
raised by the staff in its letter of comments dated September 20, 2006. Set
forth below is the Company's response to Commission's comments.

General

1.    We note your response to our prior comment number 2. Please confirm that
      you will include a fixed price, rather than a range in the final
      prospectus delivered to investors, consistent with Item 501(a)(9)(iv) of
      Regulation S-B.

      The final prospectus will replace the language relating to a price range
      with language relating to a fixed price.

Registration Statement Fee Table

2.    We have reviewed your response to our prior comment number 3; however,
      footnote (1) to your fee table continues to refer to the average exercise
      price of the warrants. Please tell us why you believe it is appropriate to
      use an average of the warrant exercise prices rather than stating each
      warrant exercise price, and the number of warrants subject to each price,
      separately.

      Footnote (1) has been revised to provide that the registration fee with
respect to all of the registered securities is based on the maximum proposed
offering price of $2.00 per share. Footnote (3) sets forth the number of shares
issuable upon exercise of the warrants at each exercise price.

Selling Stockholders, page 15

3.    We note your response to our prior comment number 10; however, we are
      unable to locate new disclosure describing the services, San Juan Holdings
      provided in exchange for the stock and warrants. Please revise or advise.

      The discussion relating to San Juan Holdings under "Selling Stockholders"
has been revised to add the following language: "The services rendered by San
Juan Holdings included advice to Plaza and to Ms. Plaza, and negotiation with us
on behalf of Plaza and Ms. Plaza, as to the structure of the transaction and the
consideration payable to Ms. Plaza for her stock in Plaza, including the amount
paid at closing, the equity to be issued to Ms. Plaza and the amount, timing and
conditions of the deferred payment."




Securities and Exchange Commission
October 4, 2006
Page 2


Principal Customers, page 29

4.    We have reviewed your response to our prior comment number 14 and we
      continue to believe that the contracts must be filed as exhibits to your
      registration statement. Item 601(b)(10)(i)(B) of Regulation S-B
      specifically requires you to file any contract upon which your business is
      substantially dependent, including contracts with principal customers. If
      you wish to request confidential treatment for portions of the contracts
      not previously publicly disclosed, you may do so under Rule 406 of
      Regulation C; however, confidential treatment will not be available for
      any terms that are material to investors or otherwise required to he
      disclosed. For further guidance, refer to Staff Legal Bulletin No. 1. In
      addition, please be advised that all comment letters and responses are
      made public on the SBC's website after we complete our review. See
      Commission Press Release dated June 24, 2004, which is available at
      www.sec.gov.

      We are filing with the amendment contracts with our four largest
customers. We are seeking confidential treatment of confidential pricing
information in certain of these contracts. The exhibits for which confidential
treatment is requested (exhibits 10.12 through 10.15) reflect the deletion of
the confidential information. The request for confidential treatment is being
made pursuant to Rule 406.

5.    In light of the preceding comment, please revise your disclosure to
      identify the customers currently identified as A, B, and C.

      We have revised the prospectus to identify our four largest customers.

Financial Statements

Report of Independent Registered Accounting Firm, page F-2

6.    We note that the audit report does not appear to be signed by your
      independent accountants. Please confirm to us that you obtained a manually
      signed audit report from your independent registered public accounting
      firm, Kevane Soto Pasarell Grant Thornton LLP, at the time of this filing,
      in accordance with Rule 302 of Regulation S-T. If a manually signed audit
      report was properly obtained, revise the report to include a conforming
      signature preceded by the /s/ designation in accordance with Rule 302 of
      Regulation S-T and as required by Article 2-02 of Regulation S-X,

      We have obtained a manually signed report from Kevane Soto Pasarell Grant
Thornton LLP, and the conforming signature is reflected in the amendment.

Statements of Income, page F-4

7.    We have reviewed your responses to prior comments 15 and 19. It appears
      that you have not filed an amended Form l0-QSB for the quarter ended
      January 31, 2006 to update your weighted average shares outstanding and
      earnings per common share amounts as you indicated in your response.
      Please file the amended report and supplementally provide us with your
      weighted average shares outstanding calculation for all periods included
      in your Forms l0-QSB for the quarters ended January 31, 2006 and April 30,
      2006.




Securities and Exchange Commission
October 4, 2006
Page 3


      For the three month periods ended January 31, 2005 and April 30, 2005 and
the six month period ended April 30, 2005, the number of shares outstanding for
basic earnings per share is 1,750,000, representing the shares of common stock
that were issued to Elizabeth Plaza and San Juan Holdings. Under reverse
acquisition accounting, these shares are treated as outstanding for periods
prior to the reverse acquisition in January 2006.

      For the three month periods ended January 31, 2005 and April 30, 2005 and
the six month period ended April 30, 2005, the number of shares outstanding for
diluted earnings per share is 4,045,752. This number is determined by adding to
the 1,750,000 shares deemed outstanding, the additional 2,295,752 shares
issuable as a result of outstanding options using the treasury stock method. The
following table sets forth the computation of these shares.




                                                                       
From exercise of San Juan Holdings Warrants:
- --------------------------------------------
Proceeds from exercise of San Juan Holdings Warrants    2,500,000    $  0.06    $ 150,000
                                                                                =========
Number of Shares Issued                                                         2,500,000
Number of Shares assumed to be reacquired                                         204,248
                                                                                ---------
Number of Shares assumed issued and not reacquired                              2,295,752
                                                                                =========

From exercise of options of Plaza key employees:
- ------------------------------------------------
Proceeds from exercise of stock options                   776,186    $0.7344    $ 570,031
                                                                                =========
Number of Shares Issued                                                           776,186
Number of Shares assumed to be reacquired                                         776,186
                                                                                ---------

Number of Shares assumed issued and not reacquired                                     --
                                                                                =========


      On the January 25, 2006, we completed the private placement of 47 units,
each unit consisting of 25,000 shares of series A preferred stock, warrants to
purchase 85,100 shares of common stock at $1.10 per share and warrants to
purchase 85,100 shares of common stock at $1.65 per share. In the private
placement, we issued an aggregate of 1,175,000 shares of series A preferred
stock, which were convertible into 15,998,800 shares of common stock, and
warrants to purchase 3,999,700 shares of common stock at $1.10 per share, and
warrants to purchase 3,999,700 shares of common stock at $1.65 per share. On
April 25, 2006, the series A preferred stock was automatically converted into
15,988,800 shares of common stock.




Securities and Exchange Commission
October 4, 2006
Page 4


      In computing basic earnings per share, in addition to the 1,750,000 shares
of common stock that were outstanding prior to the January 25, 2006 closing, the
following additional shares are included:

      o     The 551,800 shares held by the stockholders of Lawrence prior to the
            closing are treated as outstanding since January 25, 2006.

      o     The 14,401 shares that were issued as a result of our failure to
            file a registration statement in a timely manner are included from
            April 25, 2006.

      o     The 15,998,800 shares issued upon conversion of the series A
            preferred stock are included in computing basic earnings per share
            commencing April 25, 2006.

      In computing diluted earnings per share, the following shares of common
stock, in addition to the shares of common stock included in determining basic
earnings per share, are included. The shares issuable upon exercise of the
warrants are included in computing diluted earnings per share from January 25,
2006, using the treasury stock method applying the same methodology as described
above. The following are the additional shares used in computing diluted
earnings per share:

      o     The 3,999,700 shares issuable upon exercise of warrants having an
            exercise price of $1.10, which are included from January 25, 2006
            until the end of the applicable period.

      o     The 3,999,700 shares issuable upon exercise of warrants having an
            exercise price of $1.65, which are included from January 25, 2006
            until the end of the applicable period.

      o     The 1,600,000 shares issuable upon exercise of warrants having an
            exercise price of $.06 which had been issued by Lawrence prior to
            the closing, are included from January 25, 2006 until the end of the
            applicable period.

      o     The 15,998,800 shares of common stock issued upon conversion of the
            series A preferred stock are included from January 25, 2006 until
            April 25, 2006, at which time the series A preferred stock was
            converted into common stock.

      o     The 14,401 shares of common stock issued as a result of our failure
            to file a registration statement in a timely manner are included
            from January 25, 2006 until April 25, 2006, at which time the series
            A preferred stock was converted into common stock.

      o     The 1,439,892 shares issuable upon exercise of warrants having an
            exercise price of $0.7344, per share are included from January 25,
            2006 until the end of the applicable period.

      o     The 1,687,500 shares of common stock issuable upon exercise of
            options granted during the period January 25, 2006 through the end
            of the applicable periods are included from the date of grant until
            the end of the applicable period.




Securities and Exchange Commission
October 4, 2006
Page 5


      We are looking for an effective date on or about October 11, 2006. We
would appreciate any expedited treatment you could give to this filing.

      Notwithstanding the comments of the staff, we hereby acknowledge:

      o     We are responsible for the adequacy and accuracy of the disclosure
            in the filing.

      o     Staff comments or changes in response to staff comments in the
            proposed disclosure in do not foreclose the Commission from taking
            any action with respect to the filing; and

      o     We may not assert staff comment may as a defense in any proceeding
            initiated by the Commission or any person under the federal
            securities laws of the United States.

                                                         Very truly yours,

                                                         /s/ Manuel O. Morera
                                                         -----------------------
                                                         Manuel O. Morera
                                                         Chief Financial Officer