Report of Independent Registered
Public Accounting Firm

To the Board of Trustees of The Marsico
Investment Fund and Shareholders of
Marsico Focus Fund, Marsico Growth Fund,
Marsico 21st Century Fund, Marsico
International Opportunities Fund and
Marsico Global Fund

In planning and performing our audits of
the financial statements of Marsico Focus
Fund, Marsico Growth Fund, Marsico 21st
Century Fund, Marsico International
Opportunities Fund and Marsico Global
Fund (constituting The Marsico Investment
Fund, hereafter collectively referred to as
the Funds) as of and for the year ended
September 30, 2020, in accordance with the
standards of the Public Company
Accounting Oversight Board (United States)
(PCAOB), we considered the Funds internal
control over financial reporting, including
controls over safeguarding securities, as a
basis for designing our auditing procedures
for the purpose of expressing our opinion on
the financial statements and to comply with
the requirements of Form N-CEN, but not
for the purpose of expressing an opinion on
the effectiveness of the Funds internal
control over financial reporting.
Accordingly, we do not express an opinion
on the effectiveness of the Funds internal
control over financial reporting.

The management of the Funds is responsible
for establishing and maintaining effective
internal control over financial reporting. In
fulfilling this responsibility, estimates and
judgments by management are required to
assess the expected benefits and related
costs of controls. A companys internal
control over financial reporting is a process
designed to provide reasonable assurance
regarding the reliability of financial
reporting and the preparation of financial
statements for external purposes in
accordance with generally accepted
accounting principles. A companys internal
control over financial reporting includes
those policies and procedures that (1)
pertain to the maintenance of records that,
in reasonable detail, accurately and fairly
reflect the transactions and dispositions of
the assets of the company; (2) provide
reasonable assurance that transactions are
recorded as necessary to permit preparation
of financial statements in accordance with
generally accepted accounting principles,
and that receipts and expenditures of the
company are being made only in accordance
with authorizations of management and
trustees of the company; and (3) provide
reasonable assurance regarding prevention
or timely detection of unauthorized
acquisition, use or disposition of a
companys assets that could have a material
effect on the financial statements.

Because of its inherent limitations, internal
control over financial reporting may not
prevent or detect misstatements. Also,
projections of any evaluation of effectiveness
to future periods are subject to the risk that
controls may become inadequate because of
changes in conditions, or that the degree of
compliance with the policies or procedures
may deteriorate.

A deficiency in internal control over
financial reporting exists when the design or
operation of a control does not allow
management or employees, in the normal
course of performing their assigned
functions, to prevent or detect
misstatements on a timely basis. A material
weakness is a deficiency, or a combination of
deficiencies, in internal control over
financial reporting, such that there is a
reasonable possibility that a material
misstatement of the companys annual or
interim financial statements will not be
prevented or detected on a timely basis.

Our consideration of the Funds internal
control over financial reporting was for the
limited purpose described in the first
paragraph and would not necessarily
disclose all deficiencies in internal control
over financial reporting that might be
material weaknesses under standards
established by the PCAOB. However, we
noted no deficiencies in the Funds internal
control over financial reporting and their
operations, including controls over
safeguarding securities, that we consider to
be material weaknesses as defined above as
of September 30, 2020.

This report is intended solely for the
information and use of the Board of Trustees
of The Marsico Investment Fund and the
Securities and Exchange Commission and is
not intended to be and should not be used
by anyone other than these specified parties.

/s/ PricewaterhouseCoopers LLP
Denver, Colorado
November 18, 2020


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