Exhibit 16.1 April 6, 2004 Securities and Exchange Commission Washington, D.C. 20549 Ladies and Gentlemen: We were previously principal accountants for VaxGen, Inc. and, under the date of March 8, 2004, we reported on the consolidated financial statements of VaxGen, Inc. as of and for the years ended December 31, 2003 and 2002. On April 6, 2004, our appointment as principal accountants was terminated. We have read VaxGen, Inc.'s statements included under Item 4 of its Form 8-K dated April 6, 2004, and we agree with such statements except that we are not in a position to agree or disagree with VaxGen's statements regarding: a. audit committee and management actions taken in response to the deficiencies in the internal controls of the Company identified by KPMG LLP; b. whether PricewaterhouseCoopers LLP was consulted on the application of accounting principles to a specific transaction, either completed or proposed, the type of audit opinion that might be rendered on VaxGen, Inc.'s consolidated financial statements or any of the reportable events described in the Form 8-K, and; c. whether the Audit Committee of the Board of Directors ("the Audit Committee") of VaxGen, Inc., as part of its regular review of the performance of and its relationship with the independent auditors of the Company, considered KPMG LLP and two other independent accounting firms to serve as the Company's independent auditors in 2004. Very truly yours, /s/ KPMG LLP cc: Randall L-W. Caudill, Audit Committee Chairman, VaxGen, Inc. James M. Cunha, Interim Chief Financial Officer, VaxGen, Inc.