EXHIBIT 10.1


                        IRIS INTERNATIONAL, INC. ("IRIS")
                                SECOND AMENDMENT
                                       TO
                   KEY EMPLOYEE AGREEMENT FOR CESAR M. GARCIA


         IRIS INTERNATIONAL, INC., a Delaware corporation (the "COMPANY") agrees
with you to amend the Key Employee  Agreement for Cesar M. Garcia, as amended by
that  certain  First  Amendment  to Key  Employee  Agreement  (as  amended,  the
"AGREEMENT") as follows, effective as of November 14, 2007:

         1. Section 3.7 of the Agreement is deleted in its entirety and replaced
by the following:

                  "3.7 In the event that (i) you are terminated without cause at
any time,  pursuant to Section 2.2(c) hereof and (ii) you deliver to the Company
a signed settlement agreement and general release in the form attached hereto as
Exhibit  A (the  "Release")  and  satisfy  all  conditions  to make the  Release
effective,  the Company shall pay you the following: (a) an amount that does not
exceed  two times the  maximum  amount  that may be taken into  account  under a
qualified plan pursuant to section  401(a)(17) of the Internal Revenue Code (the
"Code") for the year in which such termination occurs; and (b) an amount that is
the difference between eighteen months of base salary (based on the monthly rate
of base salary in effect  immediately  prior to such termination) and the amount
determined  under  subsection (a), above;  provided,  however,  that in no event
shall the sum of the amounts  computed  under  subsections  (a) and (b),  above,
exceed  eighteen months of base salary (based on the monthly rate of base salary
in effect immediately prior to such termination).  At the choice of the Company,
payment of the amount computed under subsection (a) may be made in the form of a
lump sum  payment  within ten (10) days of the  termination  or through  regular
payroll  payments  in equal  amounts  for a period  that  begins in the month of
termination  and ends no later  than  eighteen  (18)  months  after the month of
termination, and payment of the amount computed under subsection (b) may be made
in the form of a lump sum  payment  within ten (10) days of the  termination  or
through  regular  payroll  payments in equal amounts for a period that begins in
the month of termination  and ends no later than the 15th day of the third month
of the calendar year following the year in which you are terminated. The parties
intend that the  compensation  payable pursuant to subsection (b) above shall be
treated as a  short-term  deferral  as that term is used in section  409A of the
Code and the regulations promulgated thereunder (collectively,  "SECTION 409A").
The parties intend that each of the payments payable pursuant to (a) above shall
be treated as a separate  payment for purposes of section 409A and excluded from
the  definition  of  "deferred   compensation"   pursuant  to  the   regulations
promulgated  thereunder  regarding  separation  pay payable upon an  involuntary
separation from service.  If the amount computed under  subsection (a) or (b) is
made through regular payroll  payments,  the Company shall maintain your medical
and dental  insurance  benefits in accordance with those in effect for employees
at the time of such  termination,  but only for the  longer  of the two  periods
during which such payments are to be made through regular  payroll.  Termination
without cause shall  include  "constructive  termination"  in the event of (i) a
material  diminution of your authority,  duties or responsibilities as described
in SECTION 1 above, (ii) a material breach of this Agreement by the Company,  or
(iii) the  termination  by you of your  employment  with the Company at any time





within 30 days  following the  relocation  of your primary  office to a location
more than 60 miles from your current office in Chatsworth,  California; provided
that  before any  constructive  termination  occurs,  you first give the Company
notice  of the event or other  circumstances  giving  rise to such  constructive
termination  within 90 days of the occurrence thereof and afford the Company the
right to cure the event or other circumstances  giving rise to such constructive
termination  for a period of 30 days  following  the  Company's  receipt of such
notice."

         2. Section 11 of the  Agreement is deleted in its entirety and replaced
by the following:

                  "11. COMPLETE AGREEMENT;  AMENDMENTS. The foregoing,  together
with  the  Proprietary  Information  Agreement  and  Addendum  1, is the  entire
agreement of the parties with respect to the subject  matter  hereof and thereof
and may not be amended,  supplemented,  canceled or discharged except by written
instrument executed by both parties hereto."

         3. The  Agreement  is  amended  by  adding a new  Exhibit A in the form
attached hereto as Exhibit A.

         4. This agreement may be executed in any number of  counterparts,  each
of which shall be deemed to be an original  and all of which  together  shall be
deemed to be one and the same instrument.

                                IRIS INTERNATIONAL, INC.


                                By:  /s/ Richard H. Williams
                                     ------------------------------
                                     Richard H. Williams, Chairman


                                By:  /s/ Dr. Richard Nadeau
                                     ------------------------------
                                     Dr. Richard Nadeau, Chairman
                                     of the Compensation Committee

                                Date:  November 14, 2007


Accepted and agreed this
14th day of November, 2007.


/s/ Cesar M. Garcia
- -----------------------------
Cesar M. Garcia


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                                    EXHIBIT A
                                [IRIS LETTERHEAD]
                                     RELEASE
[DATE]
EMPLOYEE NAME
ADDRESS

         RE:      SEPARATION TERMS AND GENERAL RELEASE AGREEMENT

Dear [NAME]:

This letter  confirms the terms of your  separation  from the employment of IRIS
International,  Inc. and  consideration  in exchange for your waiver and general
release  of  claims  in  favor of IRIS  International,  Inc.  and its  officers,
directors,   employees,  agents,   representatives,   subsidiaries,   divisions,
affiliated companies,  successors,  and assigns (collectively,  the "COMPANY" or
"IRIS").

         1.  TERMINATION  DATE.  Your  employment  with  the  Company  will  end
effective   _____________  (the  "TERMINATION   DATE").   Between  now  and  the
Termination  Date, you should assist with any  transition-related  activities as
directed by the employee to whom you directly report.

         2.  ACKNOWLEDGMENT  OF PAYMENT OF WAGES. On or before execution of this
release,  we delivered to you a final  paycheck  that  includes  payment for all
accrued wages, salary, accrued and unused vacation time,  reimbursable expenses,
and any  similar  payments  due and  owing  to you from  the  Company  as of the
Termination  Date  (collectively  referred to as  "WAGES").  You are entitled to
these Wages  regardless  of whether you sign this  Separation  Terms and General
Release Agreement (the "AGREEMENT").

         3.  CONSIDERATION  FOR  RELEASE.  In  consideration  of the  waiver and
release of claims set forth in  Paragraphs  7 and 8 below,  and in exchange  for
your  signing  this  Agreement,  the  Company  agrees  to  provide  you with the
post-termination payments (the "SEVERANCE PAYMENTS") described in Section 3.7 of
that  certain IRIS  International,  Inc.  Key  Employee  Agreement  for Cesar M.
Garcia, as amended to date (the "IRIS OFFER LETTER"). The Severance Payments are
in addition to any amounts owed to you by the Company. You acknowledge and agree
that you are not  otherwise  entitled to receive  the  Severance  Payments.  You
understand  that if you do not sign the  Agreement,  or if you revoke the signed
Agreement as described in Paragraph 19 below (if applicable), the Company has no
obligation to provide you with the Severance Payments.

         4. COBRA CONTINUATION  COVERAGE.  Your Company provided health coverage
will end on your  Termination  Date.  If you are eligible  for, and timely elect
COBRA  continuation,  you may continue health coverage pursuant to the terms and
conditions of COBRA at your own expense.  Our Human  Resources  Department  will
contact you shortly  after your  Termination  Date.  All other  insured  benefit
coverage  (e.g.,  life  insurance,  disability  insurance) will also end on your
Termination Date.


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         5. RETURN OF COMPANY PROPERTY. By signing below, you represent that you
have returned all the Company  property and data of any type whatsoever that was
in your possession or control.

         6. CONFIDENTIAL INFORMATION. You hereby acknowledge that as a result of
your  employment  with  the  Company  you  have  had  access  to  the  Company's
confidential information.  You acknowledge your continuing obligations under the
Employee  Confidentiality  Agreement you have previously executed, and you agree
you will hold all such confidential information in strictest confidence and that
you may not make any use of such confidential  information.  You further confirm
that you have  delivered  to the  Company all  documents  and data of any nature
containing or pertaining to such Confidential  Information and that you have not
taken with you any such documents or data or any copies thereof.

         7. GENERAL RELEASE AND WAIVER OF CLAIMS.

                  7.1. The payments and  agreements  set forth in this Agreement
         fully  satisfy  any and all accrued  salary,  vacation  pay,  bonus and
         commission pay, stock-based compensation,  profit sharing,  termination
         benefits or other  compensation  to which you may be entitled by virtue
         of your employment with the Company or your  termination of employment.
         You  acknowledge  that you have no claims and have not filed any claims
         against the Company based on your  employment with or the separation of
         your employment with the Company.

                  7.2.  To the  fullest  extent  permitted  by law,  you  hereby
         release and forever discharge the Company, its successors, subsidiaries
         and affiliates, directors,  shareholders,  current and former officers,
         agents  and  employees  (all of whom are  collectively  referred  to as
         "RELEASEES")  from any and all  existing  claims,  demands,  causes  of
         action,  damages and liabilities,  known or unknown, that you ever had,
         now have or may claim to have had arising out of or relating in any way
         to your  employment  or  separation  from  employment  with the Company
         including,  without  limitation,  claims based on any oral,  written or
         implied employment agreement,  claims for wages, bonuses,  commissions,
         stock-based  compensation,  expense reimbursement,  and any claims that
         the terms of your employment with the Company,  or the circumstances of
         your  separation,  were  wrongful,  in breach of any  obligation of the
         Company or in violation of any of your rights,  contractual,  statutory
         or  otherwise.  Each of the  Releasees  is intended to be a third party
         beneficiary  of the  General  Release and Waiver of Claims set forth in
         this Paragraph 7.

                           (a) RELEASE OF STATUTORY AND COMMON LAW CLAIMS.  Such
                  rights include,  but are not limited to, your rights under the
                  following federal and state statutes:  the Employee Retirement
                  Income Security Act (ERISA) (regarding employee benefits); the
                  Occupational  Safety  and  Health Act  (safety  matters);  the
                  Family and Medical  Leave Act of 1993;  the Worker  Adjustment
                  and Retraining  Act ("WARN")  (notification  requirements  for
                  employers  who are  curtailing  or closing an  operation)  and
                  common law; tort; wrongful discharge;  public policy; workers'
                  compensation    retaliation;    tortious   interference   with
                  contractual  relations,  misrepresentation,   fraud,  loss  of
                  consortium;   slander,  libel,   defamation,   intentional  or
                  negligent infliction of emotional distress;  claims for wages,
                  bonuses,  commissions,   stock-based  compensation  or  fringe
                  benefits;  vacation  pay; sick pay;  insurance  reimbursement,
                  medical expenses,  and the like.


                                       4



                           (b) RELEASE OF DISCRIMINATION  CLAIMS. You understand
                  that various federal,  state and local laws prohibit age, sex,
                  race, disability, benefits, pension, health and other forms of
                  discrimination,  harassment  and  retaliation,  and that these
                  laws  can  be  enforced  through  the  U.S.  Equal  Employment
                  Opportunity  Commission,  the National Labor Relations  Board,
                  the Department of Labor,  and similar state and local agencies
                  and  federal  and state  courts.  You  understand  that if you
                  believe your  treatment by the Company  violated any laws, you
                  have the right to consult  with these  agencies  and to file a
                  charge with them.  Instead,  you have decided  voluntarily  to
                  enter into this  Agreement,  release  the claims and waive the
                  right  to  recover  any  amounts  to which  you may have  been
                  entitled  under such laws,  including  but not limited to, any
                  claims   you  may  have   based  on  age  or  under   the  Age
                  Discrimination  in  Employment  Act of 1967  (ADEA;  29 U.S.C.
                  Section  621  et.  seq.)  (age);  the  Older  Workers  Benefit
                  Protection Act ("OWBPA") (age);  Title VII of the Civil Rights
                  Act of 1964 (race, color,  religion,  national origin or sex);
                  the 1991 Civil Rights Act; the Vocational  Rehabilitation  Act
                  of 1973  (disability);  The Americans with Disabilities Act of
                  1990  (disability);  42  U.S.C.  Section  1981,  1986 and 1988
                  (race); the Equal Pay Act of 1963 (prohibits pay differentials
                  based on sex); the Immigration Reform and Control Act of 1986;
                  Executive Order 11246 (race, color,  religion, sex or national
                  origin);  Executive  Order 11141  (age);  Vietnam Era Veterans
                  Readjustment  Assistance Act of 1974 (Vietnam era veterans and
                  disabled  veterans);  and California  state statutes and local
                  laws of similar effect.

                  7.3.  Releasees  and you do not intend to  release  claims (i)
         which  you may not  release  as a  matter  of law  (including,  but not
         limited to,  indemnification  claims under  applicable  law);  (ii) for
         unemployment,  state  disability  and/or  paid family  leave  insurance
         benefits  pursuant to the terms of applicable  state law; (iii) for any
         benefit  entitlements  that  are  vested  as of  the  Termination  Date
         pursuant to the terms of a  Company-sponsored  benefit plan governed by
         the  federal  law known as "ERISA";  and (iv) for vested  stock  and/or
         vested  option shares  pursuant to the written terms and  conditions of
         your existing stock and stock option grants and agreements  existing as
         of the  Termination  Date. To the fullest extent  permitted by law, any
         dispute regarding the scope of this general release shall be determined
         by an arbitrator under the procedures set forth in paragraph 12.

         8.  WAIVER OF UNKNOWN  CLAIMS.  You  expressly  waive any  benefits  of
Section 1542 of the Civil Code of the State of California (and any other laws of
similar effect), which provides:

         "A GENERAL  RELEASE DOES NOT EXTEND TO CLAIMS  WHICH THE CREDITOR  DOES
         NOT  KNOW OR  SUSPECT  TO  EXIST  IN HIS OR HER  FAVOR  AT THE  TIME OF
         EXECUTING  THE  RELEASE,  WHICH  IF  KNOWN  BY  HIM OR  HER  MUST  HAVE
         MATERIALLY AFFECTED HIS OR HER SETTLEMENT WITH THE DEBTOR."

         9. COVENANT NOT TO SUE.

                  9.1. To the fullest  extent  permitted  by law, you agree that
         you will not now or at any time in the future pursue any charge, claim,
         or action of any kind, nature and character  whatsoever  against any of
         the Releasees,  or cause or knowingly permit any such charge,  claim or


                                       5



         action  to be  pursued,  in any  federal,  state  or  municipal  court,
         administrative agency,  arbitral forum, or other tribunal,  arising out
         of any of the matters covered by paragraphs 7 and 8 above.

                  9.2.  You  further  agree  that  you will  not  pursue,  join,
         participate, encourage, or directly or indirectly assist in the pursuit
         of any legal  claims  against  the  Releasees,  whether  the claims are
         brought on your own behalf or on behalf of any other person or entity.

                  9.3.  Nothing in this  paragraph  shall prohibit you from: (1)
         providing  truthful  testimony  in  response  to a  subpoena  or  other
         compulsory legal process,  and/or (2) filing a charge or complaint with
         a  government   agency  such  as  the  Equal   Employment   Opportunity
         Commission,  the National  Labor  Relations  Board or applicable  state
         anti-discrimination agency.

         10. NON-DISPARAGEMENT.  You agree that you will not make any statement,
written  or oral,  or  engage  in any  conduct  that is or could  reasonably  be
construed to be  disparaging of the Company or its products,  services,  agents,
representatives,   directors,  officers,  shareholders,   attorneys,  employees,
vendors,  affiliates,  successors or assigns,  or any person acting by, through,
under or in concert with any of them.  Nothing in this paragraph  shall prohibit
you from  providing  truthful  testimony  in  response  to a  subpoena  or other
compulsory legal process.

         11. LEGAL AND EQUITABLE REMEDIES. You and the Company agree that either
party shall have the right to enforce this  Agreement and any of its  provisions
by injunction,  specific performance or other equitable relief without prejudice
to any other  rights or remedies  that either party may have at law or in equity
for breach of this Agreement.

         12.  ARBITRATION OF DISPUTES.  Except for claims for injunctive  relief
arising out of a breach of the Employee  Confidentiality  Agreement, you and the
Company agree to submit to mandatory  binding  arbitration  any future  disputes
between you and the Company,  including  any claim arising out of or relating to
this Agreement.  By signing below,  you and the Company waive any rights you and
the  Company  may have to trial by jury of any such  claims.  You agree that the
American  Arbitration  Association will administer any such arbitration(s) under
its  National   Rules  for  the   Resolution   of  Employment   Disputes,   with
administrative and arbitrator's fees to be borne by the Company.  The arbitrator
shall issue a written arbitration decision stating his or her essential findings
and conclusions  upon which the award is based. A party's right to review of the
decision is limited to the grounds  provided under  applicable  law. The parties
agree  that the  arbitration  award  shall be  enforceable  in any court  having
jurisdiction to enforce this Agreement.  This Agreement does not extend or waive
any statutes of  limitations  or other  provisions  of law that specify the time
within which a claim must be brought.  Notwithstanding the foregoing, each party
retains the right to seek preliminary  injunctive relief in a court of competent
jurisdiction to preserve the status quo or prevent  irreparable  injury before a
matter can be heard in arbitration.

         13.  ATTORNEYS'  FEES.  If any legal  action  arises or is  brought  to
enforce the terms of this Agreement,  the prevailing  party shall be entitled to
recover its reasonable attorneys' fees, costs and expenses from the other party,
in addition to any other relief to which such prevailing  party may be entitled,
except where the law  provides  otherwise.  The costs and  expenses  that may be
recovered exclude arbitration fees pursuant to paragraph 12 above.


                                       6



         14. CONFIDENTIALITY  PROVISION.  You agree to keep the contents,  terms
and  conditions of this Agreement  confidential  and not disclose them except to
your  spouse or  domestic  partner,  attorneys,  accountant  or as  required  by
subpoena or court order.

         15.  MATERIALITY OF BREACH.  Any breach of the provisions  contained in
paragraphs  6 through  10 and/or  14 will be  deemed a  material  breach of this
Agreement.

         16. NO ADMISSION OF LIABILITY.  You agree that this Agreement is not an
admission or evidence of any wrongdoing or liability on the part of the Company,
its  representatives,   attorneys,  agents,  partners,  officers,  shareholders,
directors,  employees,   subsidiaries,   affiliates,  divisions,  successors  or
assigns.  This Agreement will be afforded the maximum protection allowable under
California  Evidence  Code  Section  1152  and/or  any  other  state or  Federal
provisions of similar effect.

         17.  INDEMNIFICATION.  This Release shall not apply with respect to any
claims  arising  under  your  existing  rights to  indemnification  and  defense
pursuant  to (a) the  articles  and bylaws of the Company for acts as a director
and/or officer, (b) any indemnification  agreement with IRIS, or (c) your rights
of insurance under any director and officer  liability policy in effect covering
the Company's directors and officers.

         18. REVIEW OF AGREEMENT.  You may not sign this Agreement prior to your
Termination  Date.  You may take up to  twenty-one  (21)  days from the date you
receive this Agreement, or until your Termination Date, whichever date is later,
to consider this  Agreement and release and, by signing  below,  affirm that you
were  advised by this letter to consult  with an attorney  before  signing  this
Agreement and were given ample  opportunity to do so. You  understand  that this
Agreement  will not become  effective  until you return  the  original  properly
signed  Agreement  to  IRIS  Human  Resources,   attention:  Director  of  Human
Resources,   at  the  Company's  principal  executive  officers  in  Chatsworth,
California,  and after expiration of the revocation period without revocation by
you.

         [IF  EMPLOYEE  IS OVER 40 AT THE  TIME OF  TERMINATION,  THE  FOLLOWING
SECTION 19 APPLIES:

         19.  REVOCATION OF AGREEMENT.  You  acknowledge and understand that you
may revoke this  Agreement by faxing a written notice of revocation to our Human
Resources   Department,   Attention   Director  of  Human   Resources  at  (818)
_______________  any time up to seven  (7) days  after  you sign it.  After  the
revocation period has passed, however, you may no longer revoke your Agreement.

         IF  EMPLOYEE  IS  UNDER 40 AT THE TIME OF  TERMINATION,  THE  FOLLOWING
SECTION 19 APPLIES:

         19. INTENTIONALLY OMITTED.]

         20.  ENTIRE  AGREEMENT.  This  Agreement  together  with  the  Employee
Confidentiality  Agreement that you previously  executed is the entire Agreement
between you and the Company with respect to the subject matter of this Agreement
and supersedes all prior  negotiations and agreements,  whether written or oral,
relating to this subject matter.  You acknowledge that neither the Company,  nor


                                       7



its agents or attorneys, made any promise or representation, express or implied,
written or oral,  not contained in this  Agreement to induce you to execute this
Agreement.  You  acknowledge  that you have  signed  this  Agreement  knowingly,
voluntarily and without coercion, relying only on such promises, representations
and warranties as are contained in this document. You understand that you do not
waive  any  right or claim  that may arise  after  the date  this  Agreement  is
executed.

         21. MODIFICATION.  By signing below, you acknowledge your understanding
that this Agreement may not be altered, amended,  modified, or otherwise changed
in any respect except by another written agreement that  specifically  refers to
this Agreement, executed by the Company's authorized representatives and you.

         22.  GOVERNING  LAW.  This  Agreement  is  governed  by,  and  is to be
interpreted according to, the laws of the State of California.

         23. SAVINGS AND SEVERABILITY  CLAUSE.  Should any court,  arbitrator or
government  agency of competent  jurisdiction  declare or  determine  any of the
provisions  of this  Agreement  to be  illegal,  invalid or  unenforceable,  the
remaining  parts,  terms or provisions  shall not be affected  thereby and shall
remain legal, valid and enforceable. Further, it is the intention of the parties
to this  Agreement  that, if a court,  arbitrator or agency  concludes  that any
claim  under  paragraph  7 above may not be  released  as a matter  of law,  the
General  Release in paragraph 7 and the Waiver Of Unknown  Claims in paragraph 8
shall otherwise remain effective as to any and all other claims.

If this Agreement  accurately  sets forth the terms of your  separation from the
Company  and if you  voluntarily  agree to  accept  the  terms of the  severance
package  offered  please sign below no earlier  than your  Termination  Date and
return it to the Director of Human Resources.

                PLEASE REVIEW CAREFULLY. THIS AGREEMENT CONTAINS
                 A GENERAL RELEASE OF KNOWN AND UNKNOWN CLAIMS.

                                   Sincerely,



                                     [NAME]


REVIEWED, UNDERSTOOD AND AGREED:


By:
       ----------------------------------
       [NAME]
Date:
       ----------------------------------


                   DO NOT SIGN PRIOR TO YOUR TERMINATION DATE


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