UNITED STATES
SECURITIES AND EXCHANGE COMMISSION
Washington, D.C. 20549

SCHEDULE 13G

INFORMATION TO BE INCLUDED IN STATEMENTS FILED PURSUANT
TO RULE 13d-1(b), (c), AND (d) AND AMENDMENTS THERETO FILED
PURSUANT TO RULE 13d-2(b)

(AMENDMENT NO. 1)*

Quotient Ltd
(Name of Issuer)

Common Stock
(Title of Class of Securities)

G73268107
(CUSIP Number)

December 31, 2021
(Date of Event which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this
Schedule is filed:

       [    ] Rule 13d-1(b)
       [X ] Rule 13d-1(c)
       [    ] Rule 13d-1(d)

* The remainder of this cover page shall be filled out for a reporting
person's initial filing on this form with respect to the subject class
of securities, and for any subsequent amendment containing information
which would alter disclosures provided in a prior cover page.

The information required on the remainder of this cover page shall not be
deemed to be "filed" for the purpose of Section 18 of the Securities Exchange
Act of 1934 ("Act") or otherwise subject to the liabilities of that section
of the Act but shall be subject to all other provisions of the Act (however,
see the Notes).


CUSIP No. G73268107

13G

Page 2 of 9 Pages

1.
NAMES OF REPORTING PERSONS OR
I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (ENTITIES ONLY)
Pura Vida Investments, LLC


2.
CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(see instructions)

(a) [    ]
(b) [ x ]
3.
SEC USE ONLY


4.
CITIZENSHIP OR PLACE OF ORGANIZATION
Delaware



NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH
5.
SOLE VOTING POWER

 0

6.
SHARED VOTING POWER
 9,815,017*


7.
SOLE DISPOSITIVE POWER

0

8.
SHARED DISPOSITIVE POWER

 9,815,017*

9.
AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON
 9,815,017*


10.
CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES
(see instructions) [  ]


11.
PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)

9.59%**

12.
TYPE OF REPORTING PERSON (see instructions)

 IA







CUSIP No. G73268107

13G

Page 3 of 9 Pages

1.
NAMES OF REPORTING PERSONS OR
I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (ENTITIES ONLY)
Efrem Kamen


2.
CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP
(see instructions)

(a) [    ]
(b) [ x ]
3.
SEC USE ONLY


4.
CITIZENSHIP OR PLACE OF ORGANIZATION
 United States of America


NUMBER OF SHARES BENEFICIALLY OWNED BY EACH REPORTING PERSON WITH
5.
SOLE VOTING POWER

 0

6.
SHARED VOTING POWER
 9,815,017*


7.
SOLE DISPOSITIVE POWER

 0

8.
SHARED DISPOSITIVE POWER
 9,815,017*


9.
AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON
 9,815,017*


10.
CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES
(see instructions) [  ]


11.
PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9)
9.59%**


12.
TYPE OF REPORTING PERSON (see instructions)

 IN, HC








CUSIP No. G73268107

13G

Page 4 of 9 Pages

Item 1.

(a)
Name of Issuer

Quotient Ltd




(b)
Address of Issuer's Principal Executive Offices

B1, Business Park Terre Bonne
Route de Crassier 13
Eysins, Vaud 1262

Item 2.

(a)
Name of Person Filing

Pura Vida Investments, LLC
Efrem Kamen
(collectively, the "Filers").




(b)
The address of the principal place of the Filers is located at:

Pura Vida Investments, LLC
888 7th Avenue, 6th Floor
New York, NY 10106




(c)
 For citizenship of Filers, see Item 4 of the cover sheet for each Filer.




(d)
Title of Class of Securities

Common Stock




(e)
CUSIP Number

G73268107

Item 3. If this statement is filed pursuant to 240.13d-1(b) or 240.13d-2(b)
or (c), check whether the person filing is a:


(a)
[  ]
Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o).





(b)
[  ]
Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c).





(c)
[  ]
Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c).





(d)
[  ]
Investment company registered under section 8 of the Investment Company
Act of 1940 (15 U.S.C. 80a-8).



(e)
[X]
An investment adviser in accordance with 240.13d-1(b)(1)(ii)(E);





















CUSIP No. G73268107

13G

Page 5 of 9 Pages













(g)
[  ]
A parent holding company or control person in accordance with
240.13d-1(b)(1)(ii)(G);





(h)
[  ]
A savings associations as defined in Section 3(b) of the Federal Deposit
Insurance Act (12 U.S.C. 1813);





(i)
[  ]
A church plan that is excluded from the definition of an investment company
under section 3(c)(14) of the Investment Company Act of 1940
(15 U.S.C. 80a-3);





(j)
[  ]
Group, in accordance with 240.13d-1(b)(1)(ii)(J).



CUSIP No. G73268107

13G

Page 6 of 9 Pages

Item 4. Ownership.

(a) Amount beneficially owned:

Pura Vida Investments, LLC  9,815,017* shares
Efrem Kamen 9,815,017* shares (b) Percent of class:
Pura Vida Investments, LLC 9.59%** Efrem Kamen 9.59%**,(c) Number of shares as
 to which the person has:(i) Sole power
vote or to direct the vote

       0

(ii) Shared power to vote or to direct the vote

Pura Vida Investments, LLC 9,815,017* shares
Efrem Kamen 9,815,017* shares

(iii) Sole power to dispose or to direct the disposition of

       0

(iv) Shared power to dispose or to direct the disposition of

Pura Vida Investments, LLC 9,815,017* shares
Efrem Kamen 9,815,017* shares

*Shares reported herein are held by Pura Vida Master Fund, Ltd.
(the "Pura Vida Master Fund"), and certain separately managed accounts
(the "Accounts"). Pura Vida Investments, LLC ("PVI") serves as the investment
manager to the Pura Vida Master Fund and the Accounts. Efrem Kamen serves as
the managing member of PVI.
By virtue of these relationships, the Reporting Persons may be deemed to have
shared voting and dispositive power with respect to the Shares owned directly
by the Pura Vida Master Fund and the Accounts. This report shall not be deemed
an admission that the Reporting Persons are beneficial owners of the Shares
for purposes of Section 13 of the Securities Exchange Act of 1934, as amended,
or for any other purpose. Each of the Reporting Persons disclaims beneficial
ownership of the Shares reported herein except to the extent of the Reporting
Person's pecuniary interest therein.
**The percentages used herein are calculated based upon 102,293,271 Ordinary
Shares outstanding as of November 11, 2021, as reported in the Company's
Quarterly Report on Form 10-Q for the quarterly period ended
September 30, 2021 filed with the Securities and Exchange Commission on
November 16, 2021.

Item 5. Ownership of Five Percent or Less of a Class.

N/A
CUSIP No. G73268107

13G

Page 7 of 9 Pages

Item 6. Ownership of More than Five Percent on Behalf of Another Person.

N/A

Item 7. Identification and Classification of the Subsidiary Which Acquired the
Security Being Reported on By the Parent Holding Company.

N/A

Item 8. Identification and Classification of Members of the Group.

N/A

Item 9. Notice of Dissolution of Group.

N/A




CUSIP No. G73268107

13G

Page 8 of 9 Pages

Item 10. Certification.

          By signing below I certify that, to the best of my knowledge and
belief, the securities referred to above were not acquired and are not held
for the purpose of or with the effect of changing or influencing the control
of the issuer of the securities and were not acquired and are not held in
connection with or as a participant in any transaction having that purpose
or effect.



SIGNATURE


After reasonable inquiry and to the best of my knowledge and belief, I
certify that the information set forth in this statement is true, complete
and correct. Dated: February 14, 2022



Pura Vida Investments, LLC


________________________________________
By: /s/ 	Efrem Kamen, Managing Member




CUSIP No. G73268107

13G

Page 9 of 9 Pages

EXHIBIT A
AGREEMENT REGARDING JOINT FILING
OF STATEMENT ON SCHEDULE 13D OR 13G
Statement Pursuant to Rule 13d-1(k)(1)
       The undersigned hereby consent and agree to file a joint statement
on Schedule 13G under the Securities Exchange Act of 1934, as amended, with
respect to the Common Stock of Quotient Ltd beneficially owned by them,
together with any or all amendments thereto, when and if appropriate.
The parties hereto further consent this Statement pursuant to
Rule 13d-1(k)(1)(iii) as an exhibit to Schedule 13G, thereby incorporating
the same into such Schedule 13G. Dated: February 14, 2022



Pura Vida Investments, LLC



By: /s/ 	Efrem Kamen, Managing Member