185 patents
Utility
Methods and Compositions for Delivery of Molecules and Complexes to Reaction Sites
25 May 23
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites.
Jeremiah HANES, Satwik Kamtekar, Yufeng Qian
Filed: 16 Nov 22
Utility
Methods and Apparatus That Increase Sequencing-by-binding Efficiency
18 May 23
A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.
Sean STROMBERG, John VIECELI, Kandaswamy VIJAYAN, Arnold OLIPHANT
Filed: 29 Jul 22
Utility
Integrated Illumination of Optical Analytical Devices
11 May 23
Optical analytical devices and their methods of use are provided.
Paul LUNDQUIST, Stephen TURNER
Filed: 11 Jul 22
Utility
CorrectedClassification of Nucleic Acid Templates
11 May 23
Methods, compositions, and systems are provided for characterization of modified nucleic acids.
Benjamin Flusberg, Stephen Turner, Jon Sorenson, Kevin Travers, Cheryl Heiner
Filed: 26 Aug 20
Utility
Loading nucleic acids onto substrates
9 May 23
Methods, compositions, and systems for distributing nucleic acids into array regions are provided.
Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula
Filed: 15 Sep 20
Utility
Calibrated focus sensing
9 May 23
An apparatus for evaluating focus, including (a) a stage configured to hold a specimen; and (b) an optical train including a radiation source, calibration optic, objective and detector, the optical train forming a first path wherein radiation from the radiation source is directed to the calibration optic and then a first portion of the radiation is directed to the detector, thereby forming a first image on the detector, wherein a second portion of the radiation follows a second path from the calibration optic then through the objective to the specimen, wherein the optical train forms a third path wherein radiation reflected from the specimen is transmitted through the objective, then to the detector, thereby forming a second image on the detector, and wherein the radiation that forms the first image is astigmatic.
Yoshinao Taketomi, Dale Buermann, Maxim Abashin, Donald V. Rosenberry
Filed: 11 Jun 20
Utility
Method and system for biological imaging using a wide field objective lens
9 May 23
An objective lens is used for DNA sequencing.
Donald V. Rosenberry, Dale Buermann
Filed: 9 Apr 20
Utility
Illumination of optical analytical devices
2 May 23
Optical analytical devices and their methods of use are provided.
Annette Grot, Shang Wang, Hans Callebaut, Paul Lundquist, Stephen Turner
Filed: 4 Oct 21
Utility
Delaying real-time sequencing
18 Apr 23
Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest.
Tyson A. Clark, Jonas Korlach, Cheryl Heiner, Kevin Travers, Erik Miller
Filed: 21 Aug 18
Utility
Systems, Devices, and Methods for Improved Optical Waveguide Transmission and Alignment
13 Apr 23
Provided herein are systems, devices, and methods for improved optical waveguide transmission and alignment in an analytical system.
Alexey KURITSYN, Mark MCDONALD, Aaron RULISON, Russell BERMAN
Filed: 15 Aug 22
Utility
Cyanine dyes
11 Apr 23
The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes.
Stephen Yue, Gene Shen, Wei-Chuan (David) Sun
Filed: 20 Aug 19
Utility
Recombinant Polymerases with Increased Phototolerance
23 Mar 23
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing.
Satwik Kamtekar, Erik Miller, Pranav Patel
Filed: 1 Jun 22
Utility
Methods and compositions for sequencing double stranded nucleic acids using RCA and MDA
21 Mar 23
A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.
Kurt Patterson, Hari K. K. Subramanian, Brittany A. Rohrman, Fabian Block
Filed: 21 Jul 22
Utility
Flow Cells and Methods for Their Manufacture and Use
23 Feb 23
A flow cell that includes (a) a gasket interposed between a first substrate and a second substrate, wherein the gasket, the first substrate and the second substrate are impermeable to aqueous liquid and liquid adhesive, wherein the gasket has a footprint on the first substrate that delineates a channel for containing the aqueous liquid; (b) a via in the gasket, the via containing a solidified liquid adhesive that bonds the first substrate to the second substrate, wherein the solidified liquid adhesive in the via is separated from the channel by the gasket; and (c) a channel port connecting the channel to the exterior of the flow cell, wherein the channel port is permeable to the aqueous liquid.
Alex NEMIROSKI, Drew Ival FRERICHS, Julian Sean ALBERNI, Luke DAENITZ, Edgar GUTIERREZ, Arnold OLIPHANT
Filed: 2 Feb 21
Utility
Compositions and methods for selection of nucleic acids
21 Feb 23
Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis.
Thang Tat Pham, Yu-Chih Tsai, Jonas Korlach, Tyson A. Clark, Stephen Turner
Filed: 24 Mar 20
Utility
Protected fluorescent reagent compounds
14 Feb 23
Protected fluorescent reagent compounds and their methods of synthesis are provided.
Lubomir Sebo, Jeremiah Hanes, Gene Shen, Louis Brogley, Stephen Yue, Frank Zheng, Yuri Lapin, John Lyle, Honey Osuna, Andrei Fedorov
Filed: 28 Aug 20
Utility
Method of chemically modifying plastic surfaces
14 Feb 23
Disclosed herein are finished products, methods, compositions and kits for derivatizing plastic (e.g., “polymer”) surfaces in a manner that renders the surfaces appropriate for various downstream applications.
Corey M. Dambacher
Filed: 17 Jul 18
Utility
Methods and compositions for loading of polymerase complexes
14 Feb 23
The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions.
Sassan Sheikholeslami, Michael W. Hunkapiller, Natasha Popovich, Lei Sun, Erik Miller, Satwik Kamtekar
Filed: 23 Jun 20
Utility
Rapid Precipitation-driven Kilobase Size Selection of HMW Dna
9 Feb 23
Provided herein are methods of purifying a sample containing nucleic acids to obtain isolated nucleic acids of a desired size range and methods of sequencing nucleic acids of a desired size range.
Kelvin Jeng-Fang LIU, John Duncan KILBURN
Filed: 11 Dec 20
Utility
Arrays of Integrated Analytical Devices with Reduced-scale Unit Cell
2 Feb 23
Arrays of integrated analytical devices are provided.
Hans CALLEBAUT, Mark MCDONALD, Arghavan ARJMAND, Annette GROT, Mathieu FOQUET, Ravi SAXENA, Michael Tzu RU
Filed: 4 May 22