206 patents
Utility
Engineered polymerases for improved sequencing by binding
16 Jan 24
Provided herein are engineered DNA polymerases comprising modifications improving accuracy and processivity of the polymerase including modifications in the Motif A region, optionally, along with additional modifications in the palm and/or exonuclease domains of the polymerase.
Pinar Iyidogan, Mariam Iftikhar, Bridget Kidd, Lewis Churchfield
Filed: 8 Feb 22
Utility
Heterogeneous Single Cell Profiling Using Molecular Barcoding
4 Jan 24
Disclosed herein are methods of detecting at least one target biomolecule in at least one single cell comprising lysing the single cell or cells and performing a cell identification assay and target identification assay.
Niandong LIU, Norman BURNS, Manohar R. FURTADO, Bryan P. STAKER
Filed: 27 Jan 23
Utility
Illumination of Optical Analytical Devices
28 Dec 23
Optical analytical devices and their methods of use are provided.
Annette GROT, Shang WANG, Hans CALLEBAUT, Paul LUNDQUIST, Stephen TURNER
Filed: 1 May 23
Utility
Methods and Compositions for Loading of Polymerase Complexes
28 Dec 23
The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions.
Erik Miller
Filed: 2 Feb 23
Utility
Loading Nucleic Acids Onto Substrates
21 Dec 23
Methods, compositions, and systems for distributing nucleic acids into array regions are provided.
Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula, Michael W. Hunkapiller, Erik Miller, Satwik Kamtekar
Filed: 22 Mar 23
Utility
Classification of nucleic acid templates
19 Dec 23
Methods, compositions, and systems are provided for characterization of modified nucleic acids.
Benjamin Flusberg, Jonas Korlach, Andrey Kislyuk, Stephen Turner, Jon Sorenson, Kevin Travers, Cheryl Heiner, Austin B. Tomaney, Patrick Marks, Dale Webster, Jeremiah Hanes
Filed: 23 Mar 23
Utility
Methods for isolating nucleic acids
28 Nov 23
Compositions, methods and systems are provided for isolating nucleic acids.
Thang Pham, Arunashree Bhamidipati, Kevin Travers, Eric Olivares, Tyson A. Clark, Jonas Korlach
Filed: 26 Feb 21
Utility
Size Selection Purification Using a Thermoplastic Silica Nanomaterial
23 Nov 23
The present disclosure is directed to a method for purifying a sample containing nucleic acids to obtain isolated nucleic acids of a desired size range, either above a size cut-off, below a cut-off, or within a defined band of sizes, including: a) combining a nucleic acid-containing sample with a binding buffer to provide a binding mixture; b) contacting the binding mixture with a silica nanomembrane, wherein the silica nanomembrane adsorbs nucleic acids from the binding mixture within a desired size-range; and c) separating the bound nucleic acid from the remaining sample.
Kelvin Jeng-Fang LIU, John Duncan KILBURN, Jeffrey Michael BURKE
Filed: 3 Aug 23
Utility
Systems and methods for graph based mapping of nucleic acid fragments
21 Nov 23
Technical solutions for mapping long nucleic acid sequence reads to a target sequence are provided.
Ivan Sović, James Drake
Filed: 24 Jan 20
Utility
Labeled Nucleotide Analogs, Reaction Mixtures, and Methods and Systems for Sequencing
16 Nov 23
Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided.
Lubomir SEBO, Gene SHEN, Stephen YUE, Honey OSUNA, Yuri LAPIN, Louis BROGLEY, Andrei FEDOROV
Filed: 10 Oct 22
Utility
Methods and Compositions for Sequencing Double Stranded Nucleic Acids
9 Nov 23
A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.
Kurt Patterson, Hari K.K. Subramanian, Brittany A. Rohrman, Fabian Block
Filed: 16 Mar 23
Utility
FRET-labeled compounds and uses therefor
7 Nov 23
Reaction mixtures are provided having at least a first nucleotide analog and a second nucleotide analog that produce signals in response to excitation illumination.
Xiangxu Kong, Gene Shen
Filed: 25 Oct 21
Utility
Fluidic Apparatus and Methods Useful for Chemical and Biological Reactions
12 Oct 23
A reagent cartridge including (a) a support having reservoirs; (b) a main channel within the support, the channel having first and second ends exiting the support; (c) a pump channel that connects to the main channel between the first and second ends; (d) a valve manifold in the support, including (i) a first passage at the first end of the main channel, (ii) a second passage at the second end of the main channel, (iii) a first master valve between the pump channel and the first end of the main channel, (iv) a second master valve between the pump channel and the second end of the main channel, and (v) reservoir valves for regulating flow from individual reservoirs to the main channel.
Christopher Kilcoin, Kristin Dills, Rebecca McGinley
Filed: 10 Nov 22
Utility
Modified biotin-binding proteins for immobilization
10 Oct 23
Compositions comprising covalently modified and mutated biotin-binding proteins, particularly biotin-binding proteins having a negative charge at physiological pH, are provided.
Satwik Kamtekar, Lubomir Sebo, Leewin Chern, Thomas Linsky, Jeremiah Hanes, Erik Miller, Ying Yang, Stephen Yue
Filed: 6 Apr 20
Utility
Arrays of Integrated Analytical Devices
5 Oct 23
Arrays of integrated analytical devices and their methods for production are provided.
Annette GROT, Ravi SAXENA, Paul LUNDQUIST
Filed: 10 Oct 22
Utility
Systems and methods for selectively addressing sparsely arranged electronic measurement devices
3 Oct 23
A circuit comprising a substrate with sectors on the substrate is provided, each sector comprising clock and data lines, a controller in electrical communication with the clock and data lines, a counter bias line, an amplifier input line and nano-electronic measurement devices on the substrate.
Stephen Turner, Jonas Korlach, Steven Warren
Filed: 17 Dec 18
Utility
Recombinant polymerases for improved single molecule sequencing
5 Sep 23
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing.
Satwik Kamtekar, Lei Jia, Robin Emig, Erik Miller, Walter H. Lee, Molly He, Insil Park
Filed: 3 Mar 21
Utility
Fluorescent polymerase enzyme substrates having protein shields
8 Aug 23
Compositions, methods, and systems are provided for fluorescent polymerase enzyme substrates comprising protein shields for improving enzyme photostability in single molecule real time sequencing.
Keith Bjornson, Jeremiah Hanes, Erik Miller, Satwik Kamtekar, Lubomir Sebo, Louis Brogley
Filed: 14 Apr 21
Utility
Distinguishing Sequences by Detecting Polymerase Dissociation
20 Jul 23
A method for determining the presence of an allele, including (a) binding a polymerase to a double stranded nucleic acid that includes a primer hybridized to a template, the template including a first allele of a locus; (b) adding a nucleotide to the primer via catalytic activity of the polymerase, thereby producing an extended nucleic acid; (c) dissociating the polymerase from the extended nucleic acid; (d) detecting dissociation of the polymerase from the extended nucleic acid; and (e) comparing the dissociation of the polymerase from the extended nucleic acid to dissociation of the polymerase from a second double stranded nucleic acid, the second double stranded nucleic acid including a primer hybridized to the same position of the locus as the primer of the extended nucleic acid.
Denis MALYSHEV, Sean STROMBERG
Filed: 27 Sep 22
Utility
Sequencing using concatemers of copies of sense and antisense strands
18 Jul 23
Methods and systems for single molecule sequencing using concatemers of copies of sense and antisense strands.
Kevin Travers, Geoff Otto, Stephen Turner, Cheryl Heiner, Congcong Ma
Filed: 19 Jan 18