94 patents
Utility
Heterogeneous Single Cell Profiling Using Molecular Barcoding
4 Jan 24
Disclosed herein are methods of detecting at least one target biomolecule in at least one single cell comprising lysing the single cell or cells and performing a cell identification assay and target identification assay.
Niandong LIU, Norman BURNS, Manohar R. FURTADO, Bryan P. STAKER
Filed: 27 Jan 23
Utility
Illumination of Optical Analytical Devices
28 Dec 23
Optical analytical devices and their methods of use are provided.
Annette GROT, Shang WANG, Hans CALLEBAUT, Paul LUNDQUIST, Stephen TURNER
Filed: 1 May 23
Utility
Methods and Compositions for Loading of Polymerase Complexes
28 Dec 23
The present disclosure provides methods, compositions, and systems for distributing polymerase compositions into array regions.
Erik Miller
Filed: 2 Feb 23
Utility
Loading Nucleic Acids Onto Substrates
21 Dec 23
Methods, compositions, and systems for distributing nucleic acids into array regions are provided.
Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula, Michael W. Hunkapiller, Erik Miller, Satwik Kamtekar
Filed: 22 Mar 23
Utility
Size Selection Purification Using a Thermoplastic Silica Nanomaterial
23 Nov 23
The present disclosure is directed to a method for purifying a sample containing nucleic acids to obtain isolated nucleic acids of a desired size range, either above a size cut-off, below a cut-off, or within a defined band of sizes, including: a) combining a nucleic acid-containing sample with a binding buffer to provide a binding mixture; b) contacting the binding mixture with a silica nanomembrane, wherein the silica nanomembrane adsorbs nucleic acids from the binding mixture within a desired size-range; and c) separating the bound nucleic acid from the remaining sample.
Kelvin Jeng-Fang LIU, John Duncan KILBURN, Jeffrey Michael BURKE
Filed: 3 Aug 23
Utility
Labeled Nucleotide Analogs, Reaction Mixtures, and Methods and Systems for Sequencing
16 Nov 23
Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided.
Lubomir SEBO, Gene SHEN, Stephen YUE, Honey OSUNA, Yuri LAPIN, Louis BROGLEY, Andrei FEDOROV
Filed: 10 Oct 22
Utility
Methods and Compositions for Sequencing Double Stranded Nucleic Acids
9 Nov 23
A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.
Kurt Patterson, Hari K.K. Subramanian, Brittany A. Rohrman, Fabian Block
Filed: 16 Mar 23
Utility
Fluidic Apparatus and Methods Useful for Chemical and Biological Reactions
12 Oct 23
A reagent cartridge including (a) a support having reservoirs; (b) a main channel within the support, the channel having first and second ends exiting the support; (c) a pump channel that connects to the main channel between the first and second ends; (d) a valve manifold in the support, including (i) a first passage at the first end of the main channel, (ii) a second passage at the second end of the main channel, (iii) a first master valve between the pump channel and the first end of the main channel, (iv) a second master valve between the pump channel and the second end of the main channel, and (v) reservoir valves for regulating flow from individual reservoirs to the main channel.
Christopher Kilcoin, Kristin Dills, Rebecca McGinley
Filed: 10 Nov 22
Utility
Arrays of Integrated Analytical Devices
5 Oct 23
Arrays of integrated analytical devices and their methods for production are provided.
Annette GROT, Ravi SAXENA, Paul LUNDQUIST
Filed: 10 Oct 22
Utility
Distinguishing Sequences by Detecting Polymerase Dissociation
20 Jul 23
A method for determining the presence of an allele, including (a) binding a polymerase to a double stranded nucleic acid that includes a primer hybridized to a template, the template including a first allele of a locus; (b) adding a nucleotide to the primer via catalytic activity of the polymerase, thereby producing an extended nucleic acid; (c) dissociating the polymerase from the extended nucleic acid; (d) detecting dissociation of the polymerase from the extended nucleic acid; and (e) comparing the dissociation of the polymerase from the extended nucleic acid to dissociation of the polymerase from a second double stranded nucleic acid, the second double stranded nucleic acid including a primer hybridized to the same position of the locus as the primer of the extended nucleic acid.
Denis MALYSHEV, Sean STROMBERG
Filed: 27 Sep 22
Utility
Methods and Compositions for Delivery of Molecules and Complexes to Reaction Sites
25 May 23
The present invention provides methods, compositions, and systems for distributing molecules and complexes into reaction sites.
Jeremiah HANES, Satwik Kamtekar, Yufeng Qian
Filed: 16 Nov 22
Utility
Methods and Apparatus That Increase Sequencing-by-binding Efficiency
18 May 23
A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.
Sean STROMBERG, John VIECELI, Kandaswamy VIJAYAN, Arnold OLIPHANT
Filed: 29 Jul 22
Utility
Integrated Illumination of Optical Analytical Devices
11 May 23
Optical analytical devices and their methods of use are provided.
Paul LUNDQUIST, Stephen TURNER
Filed: 11 Jul 22
Utility
CorrectedClassification of Nucleic Acid Templates
11 May 23
Methods, compositions, and systems are provided for characterization of modified nucleic acids.
Benjamin Flusberg, Stephen Turner, Jon Sorenson, Kevin Travers, Cheryl Heiner
Filed: 26 Aug 20
Utility
Systems, Devices, and Methods for Improved Optical Waveguide Transmission and Alignment
13 Apr 23
Provided herein are systems, devices, and methods for improved optical waveguide transmission and alignment in an analytical system.
Alexey KURITSYN, Mark MCDONALD, Aaron RULISON, Russell BERMAN
Filed: 15 Aug 22
Utility
Recombinant Polymerases with Increased Phototolerance
23 Mar 23
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing.
Satwik Kamtekar, Erik Miller, Pranav Patel
Filed: 1 Jun 22
Utility
Flow Cells and Methods for Their Manufacture and Use
23 Feb 23
A flow cell that includes (a) a gasket interposed between a first substrate and a second substrate, wherein the gasket, the first substrate and the second substrate are impermeable to aqueous liquid and liquid adhesive, wherein the gasket has a footprint on the first substrate that delineates a channel for containing the aqueous liquid; (b) a via in the gasket, the via containing a solidified liquid adhesive that bonds the first substrate to the second substrate, wherein the solidified liquid adhesive in the via is separated from the channel by the gasket; and (c) a channel port connecting the channel to the exterior of the flow cell, wherein the channel port is permeable to the aqueous liquid.
Alex NEMIROSKI, Drew Ival FRERICHS, Julian Sean ALBERNI, Luke DAENITZ, Edgar GUTIERREZ, Arnold OLIPHANT
Filed: 2 Feb 21
Utility
Rapid Precipitation-driven Kilobase Size Selection of HMW Dna
9 Feb 23
Provided herein are methods of purifying a sample containing nucleic acids to obtain isolated nucleic acids of a desired size range and methods of sequencing nucleic acids of a desired size range.
Kelvin Jeng-Fang LIU, John Duncan KILBURN
Filed: 11 Dec 20
Utility
Arrays of Integrated Analytical Devices with Reduced-scale Unit Cell
2 Feb 23
Arrays of integrated analytical devices are provided.
Hans CALLEBAUT, Mark MCDONALD, Arghavan ARJMAND, Annette GROT, Mathieu FOQUET, Ravi SAXENA, Michael Tzu RU
Filed: 4 May 22
Utility
Paired-end Sequencing Methods and Compositions
12 Jan 23
The present invention provides methods and compositions for carrying out nucleic acid sequencing, particularly paired-end sequencing.
Jeremiah Hanes, Robert H. Reamey, Lin Wang, Fabian Block, Kurt Patterson, Brittany Ann Rohrman, Andrew Sparks, Evan Hurowitz, Joan Wilson, Min-Jui Richard Shen
Filed: 7 Jul 22