112 patents
Utility
Engineered polymerases for improved sequencing by binding
16 Jan 24
Provided herein are engineered DNA polymerases comprising modifications improving accuracy and processivity of the polymerase including modifications in the Motif A region, optionally, along with additional modifications in the palm and/or exonuclease domains of the polymerase.
Pinar Iyidogan, Mariam Iftikhar, Bridget Kidd, Lewis Churchfield
Filed: 8 Feb 22
Utility
Classification of nucleic acid templates
19 Dec 23
Methods, compositions, and systems are provided for characterization of modified nucleic acids.
Benjamin Flusberg, Jonas Korlach, Andrey Kislyuk, Stephen Turner, Jon Sorenson, Kevin Travers, Cheryl Heiner, Austin B. Tomaney, Patrick Marks, Dale Webster, Jeremiah Hanes
Filed: 23 Mar 23
Utility
Methods for isolating nucleic acids
28 Nov 23
Compositions, methods and systems are provided for isolating nucleic acids.
Thang Pham, Arunashree Bhamidipati, Kevin Travers, Eric Olivares, Tyson A. Clark, Jonas Korlach
Filed: 26 Feb 21
Utility
Systems and methods for graph based mapping of nucleic acid fragments
21 Nov 23
Technical solutions for mapping long nucleic acid sequence reads to a target sequence are provided.
Ivan Sović, James Drake
Filed: 24 Jan 20
Utility
FRET-labeled compounds and uses therefor
7 Nov 23
Reaction mixtures are provided having at least a first nucleotide analog and a second nucleotide analog that produce signals in response to excitation illumination.
Xiangxu Kong, Gene Shen
Filed: 25 Oct 21
Utility
Modified biotin-binding proteins for immobilization
10 Oct 23
Compositions comprising covalently modified and mutated biotin-binding proteins, particularly biotin-binding proteins having a negative charge at physiological pH, are provided.
Satwik Kamtekar, Lubomir Sebo, Leewin Chern, Thomas Linsky, Jeremiah Hanes, Erik Miller, Ying Yang, Stephen Yue
Filed: 6 Apr 20
Utility
Systems and methods for selectively addressing sparsely arranged electronic measurement devices
3 Oct 23
A circuit comprising a substrate with sectors on the substrate is provided, each sector comprising clock and data lines, a controller in electrical communication with the clock and data lines, a counter bias line, an amplifier input line and nano-electronic measurement devices on the substrate.
Stephen Turner, Jonas Korlach, Steven Warren
Filed: 17 Dec 18
Utility
Recombinant polymerases for improved single molecule sequencing
5 Sep 23
Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing.
Satwik Kamtekar, Lei Jia, Robin Emig, Erik Miller, Walter H. Lee, Molly He, Insil Park
Filed: 3 Mar 21
Utility
Fluorescent polymerase enzyme substrates having protein shields
8 Aug 23
Compositions, methods, and systems are provided for fluorescent polymerase enzyme substrates comprising protein shields for improving enzyme photostability in single molecule real time sequencing.
Keith Bjornson, Jeremiah Hanes, Erik Miller, Satwik Kamtekar, Lubomir Sebo, Louis Brogley
Filed: 14 Apr 21
Utility
Sequencing using concatemers of copies of sense and antisense strands
18 Jul 23
Methods and systems for single molecule sequencing using concatemers of copies of sense and antisense strands.
Kevin Travers, Geoff Otto, Stephen Turner, Cheryl Heiner, Congcong Ma
Filed: 19 Jan 18
Utility
Integrated target waveguide devices and systems for optical coupling
4 Jul 23
Integrated target waveguide devices and optical analytical systems comprising such devices are provided.
Shang Wang, Mathieu Foquet, Paul Lundquist, Aaron Rulison, Mark McDonald, Ariel Herrmann
Filed: 10 Jul 20
Utility
Calibrated focus sensing
9 May 23
An apparatus for evaluating focus, including (a) a stage configured to hold a specimen; and (b) an optical train including a radiation source, calibration optic, objective and detector, the optical train forming a first path wherein radiation from the radiation source is directed to the calibration optic and then a first portion of the radiation is directed to the detector, thereby forming a first image on the detector, wherein a second portion of the radiation follows a second path from the calibration optic then through the objective to the specimen, wherein the optical train forms a third path wherein radiation reflected from the specimen is transmitted through the objective, then to the detector, thereby forming a second image on the detector, and wherein the radiation that forms the first image is astigmatic.
Yoshinao Taketomi, Dale Buermann, Maxim Abashin, Donald V. Rosenberry
Filed: 11 Jun 20
Utility
Loading nucleic acids onto substrates
9 May 23
Methods, compositions, and systems for distributing nucleic acids into array regions are provided.
Lei Sun, Jaime Juan Benitez-Marzan, Natasha Popovich, Sassan Sheikholeslami, Steven Lin, Aparna Vedula
Filed: 15 Sep 20
Utility
Method and system for biological imaging using a wide field objective lens
9 May 23
An objective lens is used for DNA sequencing.
Donald V. Rosenberry, Dale Buermann
Filed: 9 Apr 20
Utility
Illumination of optical analytical devices
2 May 23
Optical analytical devices and their methods of use are provided.
Annette Grot, Shang Wang, Hans Callebaut, Paul Lundquist, Stephen Turner
Filed: 4 Oct 21
Utility
Delaying real-time sequencing
18 Apr 23
Methods, compositions, and systems are provided that allow for reliable sequencing of the initial sequence region of a sequence of interest.
Tyson A. Clark, Jonas Korlach, Cheryl Heiner, Kevin Travers, Erik Miller
Filed: 21 Aug 18
Utility
Cyanine dyes
11 Apr 23
The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes.
Stephen Yue, Gene Shen, Wei-Chuan (David) Sun
Filed: 20 Aug 19
Utility
Methods and compositions for sequencing double stranded nucleic acids using RCA and MDA
21 Mar 23
A method for determining sequences from sense and antisense strands of a nucleic acid, including (a) providing a nucleic acid cluster attached to a solid support, wherein the nucleic acid cluster includes a sense strand and an antisense strand of a concatemer, the concatemer including multiple copies of a sequence unit, the sequence unit including a target sequence and a primer binding site; (b) hybridizing a primer to a primer binding site in the antisense strand; (c) extending the primer along the antisense strand to determine the sequence from at least a portion of the target sequence in the antisense strand; (d) hybridizing a second primer to a primer binding site in the sense strand; and (e) extending the second primer along the sense strand to determine the sequence from at least a portion of the target sequence in the sense strand.
Kurt Patterson, Hari K. K. Subramanian, Brittany A. Rohrman, Fabian Block
Filed: 21 Jul 22
Utility
Compositions and methods for selection of nucleic acids
21 Feb 23
Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis.
Thang Tat Pham, Yu-Chih Tsai, Jonas Korlach, Tyson A. Clark, Stephen Turner
Filed: 24 Mar 20
Utility
Protected fluorescent reagent compounds
14 Feb 23
Protected fluorescent reagent compounds and their methods of synthesis are provided.
Lubomir Sebo, Jeremiah Hanes, Gene Shen, Louis Brogley, Stephen Yue, Frank Zheng, Yuri Lapin, John Lyle, Honey Osuna, Andrei Fedorov
Filed: 28 Aug 20