Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We hereby consent to the incorporation by reference in this Amendment No. 1 to the Registration Statement onForm S-3 of Proteostasis Therapeutics, Inc. of our report dated March 14, 2018 relating to the financial statements, which appears in Proteostasis Therapeutics, Inc.’s Annual Report on Form10-K for the year ended December 31, 2017. We also consent to the reference to us under the heading “Experts” in such Registration Statement.
| /s/ PricewaterhouseCoopers LLP |
| Boston, Massachusetts |
| April 19, 2018 |