Report of Independent Registered Public Accounting Firm
To the Board of Trustees of Janus Investment Fund and Shareholders of
Janus Henderson Asia Equity Fund
Janus Henderson Balanced Fund
Janus Henderson Contrarian Fund
Janus Henderson Emerging Markets Fund
Janus Henderson Enterprise Fund
Janus Henderson European Focus Fund
Janus Henderson Forty Fund
Janus Henderson Global Equity Income Fund
Janus Henderson Global Life Sciences Fund
Janus Henderson Global Real Estate Fund
Janus Henderson Global Research Fund
Janus Henderson Global Select Fund
Janus Henderson Global Sustainable Equity Fund
Janus Henderson Global Technology and Innovation Fund
Janus Henderson Growth and Income Fund
Janus Henderson International Opportunities Fund
Janus Henderson Overseas Fund
Janus Henderson Research Fund
Janus Henderson Triton Fund
Janus Henderson Venture Fund
In planning and performing our audits of the financial statements of Janus Henderson Asia Equity Fund,
Janus Henderson Balanced Fund, Janus Henderson Contrarian Fund, Janus Henderson Emerging
Markets Fund, Janus Henderson Enterprise Fund, Janus Henderson European Focus Fund, Janus
Henderson Forty Fund, Janus Henderson Global Equity Income Fund, Janus Henderson Global Life
Sciences Fund, Janus Henderson Global Real Estate Fund, Janus Henderson Global Research Fund,
Janus Henderson Global Select Fund, Janus Henderson Global Sustainable Equity Fund, Janus
Henderson Global Technology and Innovation Fund, Janus Henderson Growth and Income Fund, Janus
Henderson International Opportunities Fund, Janus Henderson Overseas Fund, Janus Henderson
Research Fund, Janus Henderson Triton Fund and Janus Henderson Venture Fund (constituting Janus
Investment Fund, hereafter collectively referred to as the “Funds") as of and for the year ended
September 30, 2021, in accordance with the standards of the Public Company Accounting Oversight
Board (United States) (PCAOB), we considered the Funds’ internal control over financial reporting,
including controls over safeguarding securities, as a basis for designing our auditing procedures for the
purpose of expressing our opinion on the financial statements and to comply with the requirements of
Form N-CEN, but not for the purpose of expressing an opinion on the effectiveness of the Funds’ internal
control over financial reporting. Accordingly, we do not express an opinion on the effectiveness of the
Funds’ internal control over financial reporting.
The management of the Funds is responsible for establishing and maintaining effective internal control
over financial reporting. In fulfilling this responsibility, estimates and judgments by management are
required to assess the expected benefits and related costs of controls. A company’s internal control over
financial reporting is a process designed to provide reasonable assurance regarding the reliability of
financial reporting and the preparation of financial statements for external purposes in accordance with
generally accepted accounting principles. A company’s internal control over financial reporting includes
those policies and procedures that (1) pertain to the maintenance of records that, in reasonable detail,
accurately and fairly reflect the transactions and dispositions of the assets of the company; (2) provide
reasonable assurance that transactions are recorded as necessary to permit preparation of financial
statements in accordance with generally accepted accounting principles, and that receipts and
expenditures of the company are being made only in accordance with authorizations of management and
directors of the company; and (3) provide reasonable assurance regarding prevention or timely detection
of unauthorized acquisition, use or disposition of a company’s assets that could have a material effect on
the financial statements.
Because of its inherent limitations, internal control over financial reporting may not prevent or detect
misstatements. Also, projections of any evaluation of effectiveness to future periods are subject to the risk
that controls may become inadequate because of changes in conditions, or that the degree of compliance
with the policies or procedures may deteriorate.
A deficiency in internal control over financial reporting exists when the design or operation of a control
does not allow management or employees, in the normal course of performing their assigned functions, to
prevent or detect misstatements on a timely basis. A material weakness is a deficiency, or a combination
of deficiencies, in internal control over financial reporting, such that there is a reasonable possibility that a
material misstatement of the company’s annual or interim financial statements will not be prevented or
detected on a timely basis.
Our consideration of the Funds’ internal control over financial reporting was for the limited purpose
described in the first paragraph and would not necessarily disclose all deficiencies in internal control over
financial reporting that might be material weaknesses under standards established by the PCAOB.
However, we noted no deficiencies in the Funds’ internal control over financial reporting and its operation,
including controls over safeguarding securities, that we consider to be a material weakness as defined
above as of September 30, 2021.
This report is intended solely for the information and use of the Board of Trustees of Janus Investment
Fund and the Securities and Exchange Commission and is not intended to be and should not be used by
anyone other than these specified parties.
PricewaterhouseCoopers LLP
Denver, Colorado
November 17, 2021