| COLUMN 1 | COLUMN 2 | COLUMN 3 | COLUMN 4 | COLUMN 5 | COLUMN 6 | COLUMN 7 | COLUMN 8 |
| | | VALUE | SHRS OR | SH/ | PUT/ | INVESTMENT | OTHER | VOTING AUTHORITY |
| NAME OF ISSUER | TITLE OF CLASS | CUSIP | (x$1000) | PRN AMT | PRN | CALL | DISCRETION | MANAGER | SOLE | SHARED | NONE |
| 10X GENOMICS INC | CL A COM | 88025U109 | 8,279 | 92,700 | SH | | SOLE | | 0 | 0 | 92,700 |
| 89BIO INC | COM | 282559103 | 79,809 | 4,004,442 | SH | | SOLE | | 4,004,442 | 0 | 0 |
| 9 METERS BIOPHARMA INC | COM | 654405109 | 14,591 | 25,716,755 | SH | | SOLE | | 25,716,755 | 0 | 0 |
| ABBVIE INC | COM | 00287Y109 | 45,556 | 464,000 | SH | | DFND | 1 | 65,100 | 0 | 398,900 |
| ABBVIE INC | COM | 00287Y109 | 9,818 | 100,000 | SH | Call | DFND | 1 | 31,500 | 0 | 68,500 |
| ACADIA HEALTHCARE COMPANY IN | COM | 00404A109 | 34,670 | 1,380,160 | SH | | DFND | 1 | 164,500 | 0 | 1,215,660 |
| ACCELERON PHARMA INC | COM | 00434H108 | 12,147 | 127,500 | SH | | DFND | 1 | 4,000 | 0 | 123,500 |
| ADVERUM BIOTECHNOLOGIES INC | COM | 00773U108 | 68,711 | 3,290,744 | SH | | DFND | 1 | 357,697 | 0 | 2,933,047 |
| AEGLEA BIOTHERAPEUTICS INC | COM | 00773J103 | 37,144 | 4,015,524 | SH | | SOLE | | 4,015,524 | 0 | 0 |
| AERPIO PHARMACEUTICALS INC | COM | 00810B105 | 6,492 | 5,193,946 | SH | | SOLE | | 5,193,946 | 0 | 0 |
| AGIOS PHARMACEUTICALS INC | COM | 00847X104 | 92,436 | 1,728,421 | SH | | DFND | 1 | 207,500 | 0 | 1,520,921 |
| AGIOS PHARMACEUTICALS INC | COM | 00847X104 | 5,348 | 100,000 | SH | Call | DFND | 1 | 32,000 | 0 | 68,000 |
| AKOUOS INC | COM | 00973J101 | 7,088 | 315,000 | SH | | SOLE | | 0 | 0 | 315,000 |
| ALCON AG | ORD SHS | H01301128 | 20,168 | 351,380 | SH | | DFND | 1 | 125,800 | 0 | 225,580 |
| ALECTOR INC | COM | 014442107 | 146,037 | 5,975,322 | SH | | SOLE | | 5,975,322 | 0 | 0 |
| ALEXION PHARMACEUTICALS INC | COM | 015351109 | 241,989 | 2,156,000 | SH | | DFND | 1 | 311,300 | 0 | 1,844,700 |
| ALEXION PHARMACEUTICALS INC | COM | 015351109 | 11,224 | 100,000 | SH | Call | DFND | 1 | 32,000 | 0 | 68,000 |
| ALPINE IMMUNE SCIENCES INC | COM | 02083G100 | 35,645 | 3,670,955 | SH | | SOLE | | 3,670,955 | 0 | 0 |
| AMGEN INC | COM | 031162100 | 21,581 | 91,500 | SH | | SOLE | | 0 | 0 | 91,500 |
| ANAPTYSBIO INC | COM | 032724106 | 8,395 | 375,800 | SH | | DFND | 1 | 35,200 | 0 | 340,600 |
| APPLIED THERAPEUTICS INC | COM | 03828A101 | 44,019 | 1,217,678 | SH | | DFND | 1 | 58,532 | 0 | 1,159,146 |
| APTOSE BIOSCIENCES INC | COM NEW | 03835T200 | 6,000 | 950,841 | SH | | DFND | 1 | 169,700 | 0 | 781,141 |
| ARCTURUS THERAPEUTICS HLDG I | COM | 03969T109 | 11,180 | 239,186 | SH | | DFND | 1 | 48,026 | 0 | 191,160 |
| ARCUS BIOSCIENCES INC | COM | 03969F109 | 5,451 | 220,350 | SH | | DFND | 1 | 26,900 | 0 | 193,450 |
| ARCUTIS BIOTHERAPEUTICS INC | COM | 03969K108 | 141,337 | 4,673,850 | SH | | DFND | 1 | 4,067,564 | 0 | 606,286 |
| ARENA PHARMACEUTICALS INC | COM NEW | 040047607 | 4,199 | 66,700 | SH | | SOLE | | 0 | 0 | 66,700 |
| ARROWHEAD PHARMACEUTICALS IN | COM | 04280A100 | 2,941 | 68,100 | SH | | SOLE | | 0 | 0 | 68,100 |
| ARVINAS INC | COM | 04335A105 | 986 | 29,400 | SH | | SOLE | | 0 | 0 | 29,400 |
| ASCENDIS PHARMA A S | SPONSORED ADR | 04351P101 | 231,166 | 1,562,986 | SH | | DFND | 1 | 1,287,486 | 0 | 275,500 |
| ATHENEX INC | COM | 04685N103 | 55,169 | 4,009,346 | SH | | DFND | 1 | 229,000 | 0 | 3,780,346 |
| ATRICURE INC | COM | 04963C209 | 55,207 | 1,228,189 | SH | | DFND | 1 | 187,000 | 0 | 1,041,189 |
| AURINIA PHARMACEUTICALS INC | COM | 05156V102 | 19,441 | 1,196,347 | SH | | SOLE | | 0 | 0 | 1,196,347 |
| AVIDITY BIOSCIENCES INC | COM | 05370A108 | 5,652 | 200,000 | SH | | SOLE | | 0 | 0 | 200,000 |
| AVROBIO INC | COM | 05455M100 | 13,850 | 793,712 | SH | | DFND | 1 | 129,000 | 0 | 664,712 |
| BELLUS HEALTH INC NEW | COM NEW | 07987C204 | 53,001 | 5,137,216 | SH | | DFND | 1 | 3,954,800 | 0 | 1,182,416 |
| BENEFYTT TECHNOLOGIES INC | COM CL A | 08182C106 | 19,701 | 962,900 | SH | | DFND | 1 | 160,400 | 0 | 802,500 |
| BIOGEN INC | COM | 09062X103 | 191,539 | 715,900 | SH | | DFND | 1 | 79,200 | 0 | 636,700 |
| BIOLINERX LTD | SPONSORED ADS | 09071M205 | 118 | 69,240 | SH | | SOLE | | 69,240 | 0 | 0 |
| BIOMX INC | COM | 09090D103 | 12,770 | 2,313,489 | SH | | SOLE | | 2,313,489 | 0 | 0 |
| BOSTON SCIENTIFIC CORP | COM | 101137107 | 170,189 | 4,847,300 | SH | | DFND | 1 | 939,400 | 0 | 3,907,900 |
| BRISTOL-MYERS SQUIBB CO | COM | 110122108 | 169,473 | 2,882,200 | SH | | DFND | 1 | 450,300 | 0 | 2,431,900 |
| BRISTOL-MYERS SQUIBB CO | RIGHT 99/99/9999 | 110122157 | 1,253 | 350,000 | SH | | DFND | 1 | 120,140 | 0 | 229,860 |
| BURNING ROCK BIOTECH LTD | SPONSORED ADS | 12233L107 | 128,191 | 4,746,071 | SH | | DFND | 1 | 523,598 | 0 | 4,222,473 |
| CALLIDITAS THERAPEUTICS AB | SPONSERED ADS | 13124Q106 | 5,220 | 225,000 | SH | | SOLE | | 0 | 0 | 225,000 |
| CELLDEX THERAPEUTICS INC NEW | COM NEW | 15117B202 | 8,125 | 625,000 | SH | | DFND | 1 | 84,800 | 0 | 540,200 |
| CENTENE CORP DEL | COM | 15135B101 | 84,884 | 1,335,700 | SH | | DFND | 1 | 179,300 | 0 | 1,156,400 |
| CIGNA CORP NEW | COM | 125523100 | 82,510 | 439,700 | SH | | DFND | 1 | 84,900 | 0 | 354,800 |
| CORBUS PHARMACEUTICALS HLDGS | COM | 21833P103 | 2,937 | 350,000 | SH | Put | DFND | 1 | 126,500 | 0 | 223,500 |
| CORVUS PHARMACEUTICALS INC | COM | 221015100 | 18,408 | 6,767,584 | SH | | SOLE | | 6,767,584 | 0 | 0 |
| CRINETICS PHARMACEUTICALS IN | COM | 22663K107 | 48,242 | 2,753,567 | SH | | SOLE | | 2,753,567 | 0 | 0 |
| CRISPR THERAPEUTICS AG | NAMEN AKT | H17182108 | 51,261 | 697,524 | SH | | DFND | 1 | 56,200 | 0 | 641,324 |
| CTI BIOPHARMA CORP | COM | 12648L601 | 11,044 | 9,520,600 | SH | | SOLE | | 9,520,600 | 0 | 0 |
| CURIS INC | COM NEW | 231269200 | 1,502 | 1,241,494 | SH | | SOLE | | 0 | 0 | 1,241,494 |
| DECIPHERA PHARMACEUTICALS IN | COM | 24344T101 | 67,227 | 1,125,700 | SH | | DFND | 1 | 190,100 | 0 | 935,600 |
| DEXCOM INC | COM | 252131107 | 91,783 | 226,400 | SH | | DFND | 1 | 33,500 | 0 | 192,900 |
| EDWARDS LIFESCIENCES CORP | COM | 28176E108 | 36,656 | 530,400 | SH | | DFND | 1 | 83,400 | 0 | 447,000 |
| EHEALTH INC | COM | 28238P109 | 62,732 | 638,558 | SH | | DFND | 1 | 86,021 | 0 | 552,537 |
| ESSA PHARMA INC | COM NEW | 29668H708 | 9,125 | 1,471,853 | SH | | SOLE | | 1,471,853 | 0 | 0 |
| EXELIXIS INC | COM | 30161Q104 | 57,073 | 2,404,100 | SH | | DFND | 1 | 288,800 | 0 | 2,115,300 |
| FLEXION THERAPEUTICS INC | COM | 33938J106 | 22,145 | 1,684,000 | SH | | DFND | 1 | 84,100 | 0 | 1,599,900 |
| FLUIDIGM CORP DEL | COM | 34385P108 | 11,657 | 2,907,100 | SH | | DFND | 1 | 341,700 | 0 | 2,565,400 |
| FORTE BIOSCIENCES INC | COM | 34962G109 | 13,856 | 950,316 | SH | | DFND | 1 | 375,301 | 0 | 575,015 |
| FUSION PHARMACEUTICALS INC | COM | 36118A100 | 56,337 | 3,224,770 | SH | | DFND | 1 | 2,930,670 | 0 | 294,100 |
| GENMARK DIAGNOSTICS INC | COM | 372309104 | 10,768 | 732,000 | SH | | DFND | 1 | 62,200 | 0 | 669,800 |
| GILEAD SCIENCES INC | COM | 375558103 | 14,788 | 192,200 | SH | | SOLE | | 0 | 0 | 192,200 |
| GLAUKOS CORP | COM | 377322102 | 46,527 | 1,211,000 | SH | | SOLE | | 1,211,000 | 0 | 0 |
| HARPOON THERAPEUTICS INC | COM | 41358P106 | 30,473 | 1,835,704 | SH | | DFND | 1 | 1,020,704 | 0 | 815,000 |
| HEALTH CATALYST INC | COM | 42225T107 | 5,239 | 179,598 | SH | | SOLE | | 179,598 | 0 | 0 |
| HERON THERAPEUTICS INC | COM | 427746102 | 9,715 | 660,420 | SH | | SOLE | | 0 | 0 | 660,420 |
| HORIZON THERAPEUTICS PUB LTD | SHS | G46188101 | 159,398 | 2,867,910 | SH | | DFND | 1 | 357,500 | 0 | 2,510,410 |
| HUMANA INC | COM | 444859102 | 108,686 | 280,300 | SH | | DFND | 1 | 42,300 | 0 | 238,000 |
| IGM BIOSCIENCES INC | COM | 449585108 | 5,895 | 80,760 | SH | | DFND | 1 | 7,460 | 0 | 73,300 |
| IMARA INC | COM | 45249V107 | 75,151 | 2,719,902 | SH | | DFND | 1 | 2,532,402 | 0 | 187,500 |
| IMMUNOMEDICS INC | COM | 452907108 | 26,860 | 757,900 | SH | | SOLE | | 0 | 0 | 757,900 |
| IMMUNOVANT INC | COM | 45258J102 | 8,634 | 354,575 | SH | | DFND | 1 | 84,400 | 0 | 270,175 |
| INFLARX NV | COM | N44821101 | 3,114 | 678,525 | SH | | DFND | 1 | 122,500 | 0 | 556,025 |
| INSPIRE MED SYS INC | COM | 457730109 | 30,196 | 347,000 | SH | | SOLE | | 347,000 | 0 | 0 |
| INTELLIA THERAPEUTICS INC | COM | 45826J105 | 2,882 | 137,100 | SH | | SOLE | | 137,100 | 0 | 0 |
| INTUITIVE SURGICAL INC | COM NEW | 46120E602 | 64,277 | 112,800 | SH | | DFND | 1 | 18,500 | 0 | 94,300 |
| IOVANCE BIOTHERAPEUTICS INC | COM | 462260100 | 182,408 | 6,645,100 | SH | | DFND | 1 | 1,337,800 | 0 | 5,307,300 |
| IQVIA HLDGS INC | COM | 46266C105 | 24,829 | 175,000 | SH | | SOLE | | 0 | 0 | 175,000 |
| IVERIC BIO INC | COM | 46583P102 | 16,575 | 3,250,000 | SH | | DFND | 1 | 609,756 | 0 | 2,640,244 |
| KALA PHARMACEUTICALS INC | COM | 483119103 | 56,783 | 5,402,730 | SH | | DFND | 1 | 4,651,895 | 0 | 750,835 |
| KEROS THERAPEUTICS INC | COM | 492327101 | 62,995 | 1,679,417 | SH | | DFND | 1 | 1,268,734 | 0 | 410,683 |
| KITOV PHARMA LTD | SPONSORED ADS | 49803V107 | 3,843 | 3,461,983 | SH | | SOLE | | 3,461,983 | 0 | 0 |
| LARIMAR THERAPEUTICS INC | COM | 517125100 | 12,001 | 933,960 | SH | | DFND | 1 | 201,637 | 0 | 732,323 |
| LOGICBIO THERAPEUTICS INC | COM | 54142F102 | 54,709 | 6,466,783 | SH | | SOLE | | 6,466,783 | 0 | 0 |
| MEIRAGTX HLDGS PLC | COM | G59665102 | 46,497 | 3,713,820 | SH | | DFND | 1 | 133,500 | 0 | 3,580,320 |
| MENLO THERAPEUTICS INC | COM | 586858102 | 1,027 | 593,883 | SH | | SOLE | | 0 | 0 | 593,883 |
| MERCK & CO. INC | COM | 58933Y105 | 200,215 | 2,589,100 | SH | | DFND | 1 | 382,100 | 0 | 2,207,000 |
| MERSANA THERAPEUTICS INC | COM | 59045L106 | 44,694 | 1,910,008 | SH | | DFND | 1 | 75,500 | 0 | 1,834,508 |
| MIRATI THERAPEUTICS INC | COM | 60468T105 | 151,172 | 1,324,100 | SH | | DFND | 1 | 195,500 | 0 | 1,128,600 |
| NABRIVA THERAPEUTICS PLC | SHS | G63637105 | 3,133 | 4,644,600 | SH | | SOLE | | 4,644,600 | 0 | 0 |
| NANOSTRING TECHNOLOGIES INC | COM | 63009R109 | 34,997 | 1,192,400 | SH | | DFND | 1 | 156,400 | 0 | 1,036,000 |
| NATERA INC | COM | 632307104 | 161,572 | 3,240,514 | SH | | DFND | 1 | 882,714 | 0 | 2,357,800 |
| NEUROCRINE BIOSCIENCES INC | COM | 64125C109 | 144,682 | 1,185,915 | SH | | DFND | 1 | 137,470 | 0 | 1,048,445 |
| NEXTCURE INC | COM | 65343E108 | 58,124 | 2,711,013 | SH | | SOLE | | 2,711,013 | 0 | 0 |
| NOVO-NORDISK A S | ADR | 670100205 | 21,608 | 330,000 | SH | | SOLE | | 0 | 0 | 330,000 |
| NOVUS THERAPEUTICS INC | COM | 67011N105 | 1,459 | 3,183,314 | SH | | SOLE | | 3,183,314 | 0 | 0 |
| OBSEVA SA | COM | H5861P103 | 15,347 | 2,605,531 | SH | | SOLE | | 2,605,531 | 0 | 0 |
| ORIC PHARMACEUTICALS INC | COM | 68622P109 | 98,558 | 2,921,967 | SH | | DFND | 1 | 2,765,717 | 0 | 156,250 |
| PASSAGE BIO INC | COM | 702712100 | 190,296 | 6,962,899 | SH | | DFND | 1 | 5,947,323 | 0 | 1,015,576 |
| PREVAIL THERAPEUTICS INC | COM | 74140Y101 | 205,955 | 13,822,463 | SH | | SOLE | | 13,822,463 | 0 | 0 |
| PROGYNY INC | COM | 74340E103 | 8,801 | 341,004 | SH | | DFND | 1 | 39,000 | 0 | 302,004 |
| PROTHENA CORP PLC | SHS | G72800108 | 37,075 | 3,544,475 | SH | | DFND | 1 | 613,800 | 0 | 2,930,675 |
| REGENERON PHARMACEUTICALS | COM | 75886F107 | 19,271 | 30,900 | SH | | SOLE | | 0 | 0 | 30,900 |
| REPARE THERAPEUTICS INC | COM | 760273102 | 106,285 | 3,426,325 | SH | | DFND | 1 | 3,276,325 | 0 | 150,000 |
| RESTORBIO INC | COM | 76133L103 | 10,385 | 4,830,387 | SH | | SOLE | | 4,830,387 | 0 | 0 |
| ROCKET PHARMACEUTICALS INC | COM | 77313F106 | 8,004 | 382,400 | SH | | DFND | 1 | 65,600 | 0 | 316,800 |
| SAREPTA THERAPEUTICS INC | COM | 803607100 | 89,734 | 559,649 | SH | | DFND | 1 | 68,000 | 0 | 491,649 |
| SCPHARMACEUTICALS INC | COM | 810648105 | 35,307 | 4,797,148 | SH | | SOLE | | 4,797,148 | 0 | 0 |
| SELECTA BIOSCIENCES INC | COM | 816212104 | 5,947 | 2,093,869 | SH | | SOLE | | 2,093,869 | 0 | 0 |
| SELECTQUOTE INC | COM | 816307300 | 31,789 | 1,255,000 | SH | | DFND | 1 | 167,900 | 0 | 1,087,100 |
| SI BONE INC | COM | 825704109 | 22,018 | 1,381,300 | SH | | DFND | 1 | 76,000 | 0 | 1,305,300 |
| SIERRA ONCOLOGY INC | COM NEW | 82640U404 | 17,876 | 1,477,320 | SH | | DFND | 1 | 1,306,860 | 0 | 170,460 |
| SINOVAC BIOTECH LTD | SHS | P8696W104 | 25,500 | 2,839,500 | SH | | SOLE | | 0 | 0 | 2,839,500 |
| SPRINGWORKS THERAPEUTICS INC | COM | 85205L107 | 311,065 | 7,406,307 | SH | | SOLE | | 7,406,307 | 0 | 0 |
| SYNDAX PHARMACEUTICALS INC | COM | 87164F105 | 16,215 | 1,094,100 | SH | | DFND | 1 | 78,600 | 0 | 1,015,500 |
| SYNLOGIC INC | COM | 87166L100 | 4,121 | 2,029,996 | SH | | SOLE | | 2,029,996 | 0 | 0 |
| TELA BIO INC | COM | 872381108 | 39,237 | 3,027,542 | SH | | SOLE | | 3,027,542 | 0 | 0 |
| TEVA PHARMACEUTICAL INDS LTD | SPONSORED ADS | 881624209 | 23,861 | 1,935,200 | SH | | DFND | 1 | 269,500 | 0 | 1,665,700 |
| THERAVANCE BIOPHARMA INC | COM | G8807B106 | 53,618 | 2,554,470 | SH | | DFND | 1 | 244,054 | 0 | 2,310,416 |
| THERMO FISHER SCIENTIFIC INC | COM | 883556102 | 39,966 | 110,300 | SH | | DFND | 1 | 16,800 | 0 | 93,500 |
| TRICIDA INC | COM | 89610F101 | 264,200 | 9,614,280 | SH | | SOLE | | 9,614,280 | 0 | 0 |
| TRILLIUM THERAPEUTICS INC | COM NEW | 89620X506 | 9,797 | 1,211,000 | SH | | DFND | 1 | 391,800 | 0 | 819,200 |
| TURNING POINT THERAPEUTICS I | COM | 90041T108 | 177,321 | 2,745,327 | SH | | DFND | 1 | 2,027,550 | 0 | 717,777 |
| UNIQURE NV | SHS | N90064101 | 43,667 | 969,079 | SH | | DFND | 1 | 138,100 | 0 | 830,979 |
| VAXCYTE INC | COM | 92243G108 | 26,869 | 850,000 | SH | | SOLE | | 0 | 0 | 850,000 |
| VERONA PHARMA PLC | SPONSORED ADS | 925050106 | 5,852 | 1,250,396 | SH | | SOLE | | 1,250,396 | 0 | 0 |
| VERRICA PHARMACEUTICALS INC | COM | 92511W108 | 16,292 | 1,479,733 | SH | | SOLE | | 1,479,733 | 0 | 0 |
| VERTEX PHARMACEUTICALS INC | COM | 92532F100 | 193,375 | 666,100 | SH | | DFND | 1 | 113,100 | 0 | 553,000 |
| X4 PHARMACEUTICALS INC | COM | 98420X103 | 10,126 | 1,086,493 | SH | | SOLE | | 1,086,493 | 0 | 0 |
| XENON PHARMACEUTICALS INC | COM | 98420N105 | 31,324 | 2,497,917 | SH | | DFND | 1 | 786,612 | 0 | 1,711,305 |
| XTANT MED HLDGS INC | COM NEW | 98420P308 | 8,324 | 9,248,678 | SH | | SOLE | | 9,248,678 | 0 | 0 |
| ZYMEWORKS INC | COM | 98985W102 | 21,004 | 582,302 | SH | | DFND | 1 | 15,300 | 0 | 567,002 |
