Nanosphere 10-Q 2008 Q2 Quarterly report

UNITED STATES SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

Form 10-Q

(Mark One)

þ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

For the quarterly period ended June 30, 2008

OR

o TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934

For the transition period from                    to                    

Commission File Number 001-33775

Nanosphere, Inc.

(Exact name of registrant as specified in its charter)

Delaware	36-4339870
(State or other jurisdiction of incorporation or organization)	(I.R.S. Employer Identification No.)
4088 Commercial Avenue	Northbrook, Illinois 60062
(Address of principal executive offices)	(Zip Code)

Registrant’s telephone number, including area code:(847) 400-9000

Indicate by check mark whether the registrant (1) has filed all reports required to be filed by Section 13 or 15(d) of the Securities Exchange Act of 1934 during the preceding 12 months (or for such shorter period that the registrant was required to file such reports), and (2) has been subject to such filing requirements for the past 90 days. Yesþ Noo

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer or a smaller reporting company. See definitions of “large accelerated filer”, “accelerated filer” and “smaller reporting company” in Rule 12b-2 of the Exchange Act. (Check one):

Large accelerated filero Accelerated filero Non-accelerated filerþ Smaller reporting companyo

Indicate by check mark whether the registrant is a shell company (as defined in Rule 12b-2 of the Exchange Act).
Yeso Noþ

The number of outstanding shares of the registrant’s common stock, par value $0.01 per share, as of August 11, 2008 was 22,228,496.

NANOSPHERE, INC.

INDEX

Part I. FINANCIAL INFORMATION
Item 1. Financial Statements (Unaudited)
Balance Sheets	1
Statements of Operations	2
Statements of Cash Flows	3
Notes to Financial Statements	4-10
Item 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations	11-16
Item 3. Quantitative and Qualitative Disclosures About Market Risk	16
Item 4. Controls and Procedures	16
Part II. OTHER INFORMATION
Item 1. Legal Proceedings	17
Item 1A. Risk Factors	17
Item 2. Unregistered Sales of Equity Securities and Use of Proceeds	17
Item 4. Submission of Matters to a Vote of Security Holders	17
Item 6. Exhibits	18
Exhibit 31.1
Exhibit 31.2
Exhibit 32.1
Exhibit 32.2

PART I.
FINANCIAL INFORMATION

Item 1. Financial Statements

Nanosphere, Inc.
(A Development Stage Company)

Balance Sheets

(Unaudited)

	June 30,			December 31,
	2008			2007
CURRENT ASSETS:
Cash and cash equivalents	$	93,990,208		$	114,312,573
Accounts receivable		482,023			100,799
Inventories		2,561,433			1,903,004
Other current assets		610,169			670,402
Total current assets		97,643,833			116,986,778
PROPERTY AND EQUIPMENT— Net		7,456,955			7,033,597
INTANGIBLE ASSETS — Net of accumulated amortization		5,090,680			4,930,752
OTHER ASSETS		144,556			163,553
TOTAL	$	110,336,024		$	129,114,680
CURRENT LIABILITIES:
Accounts payable	$	2,491,649		$	2,221,522
Accrued compensation		1,032,677			1,303,355
Accrued license fees		675,168			393,473
Accrued financing costs		—			778,731
Other current liabilities		1,011,811			969,962
Long term debt — current portion		4,532,983			3,597,823
Lease payable — current portion		40,518			37,037
Total current liabilities		9,784,806			9,301,903
LONG-TERM LIABILITIES:
Lease payable — noncurrent portion		—			21,433
Long term debt — noncurrent portion		5,495,718			7,440,804
Total liabilities		15,280,524			16,764,140
COMMITMENTS AND CONTINGENCIES
STOCKHOLDERS’ EQUITY:
Common stock, $0.01 par value; 100,000,000 shares authorized; 22,228,496 shares and 22,192,005 shares issued and outstanding as of June 30, 2008 and December 31, 2007, respectively		222,285			305,174
Preferred stock, $0.01 par value; 10,000,000 shares authorized; no shares issued		—			—
Additional paid-in capital		273,093,161			271,765,776
Warrants to acquire common stock		5,423,771			5,424,551
Deficit accumulated during the development stage		(183,683,717	)		(165,144,961	)
Total stockholders’ equity		95,055,500			112,350,540
TOTAL	$	110,336,024		$	129,114,680

See notes to financial statements.

1

Nanosphere, Inc.
(A Development Stage Company)

Statements of Operations

(Unaudited)

													Period From
													December 30, 1999
													(Date of Incorporation)
	Three Month Periods Ended June 30,						Six Month Periods Ended June 30,						Through
	2008			2007			2008			2007			June 30, 2008
REVENUE:
Grant and contract revenue	$	—		$	503,245		$	272,612		$	726,503		$	9,090,240
Product sales		194,715			7,025			498,119			53,670			907,139
Total revenue		194,715			510,270			770,731			780,173			9,997,379
COSTS AND EXPENSES:
Cost of product sales		226,176			3,188			575,605			18,367			834,573
Research and development		6,028,926			5,277,939			11,871,121			10,219,047			90,606,312
Sales, general, and administrative		3,777,507			3,079,443			7,186,405			5,256,583			39,928,861
Total costs and expenses		10,032,609			8,360,570			19,633,131			15,493,997			131,369,746
Loss from operations		(9,837,894	)		(7,850,300	)		(18,862,400	)		(14,713,824	)		(121,372,367	)
OTHER INCOME (EXPENSE):
Change in fair value of convertible derivative liability		—			—			—			—			(17,777,723	)
Change in fair value of preferred stock warrants		—			(379,599	)		—			(276,612	)		(4,551,236	)
Foreign exchange loss		(235	)		(13,770	)		(24,645	)		(13,770	)		(77,007	)
Interest expense — related party		—			—			—			—			(388,804	)
Interest expense		(545,591	)		(563,840	)		(1,130,628	)		(823,748	)		(3,115,753	)
Interest income		546,698			374,355			1,478,917			758,433			5,301,254
Total other income (expense)		872			(582,854	)		323,644			(355,697	)		(20,609,269	)
NET LOSS		(9,837,022	)		(8,433,154	)		(18,538,756	)		(15,069,521	)		(141,981,636	)
Accumulated convertible preferred stock dividends		—			(1,689,812	)		—			(3,180,329	)		(10,300,417	)
Convertible preferred stock redemption value adjustment		—			—			—			(608,940	)		(31,401,664	)
NET LOSS ATTRIBUTABLE TO COMMON STOCK	$	(9,837,022	)	$	(10,122,966	)	$	(18,538,756	)	$	(18,858,790	)	$	(183,683,717	)
Net loss per common share — basic and diluted	$	(0.44	)	$	(10.85	)	$	(0.84	)	$	(20.22	)
Weighted average number of common shares outstanding — basic and diluted		22,202,620			932,646			22,197,395			932,646

See notes to financial statements.

2

Nanosphere, Inc.
(A Development Stage Company)

Statements of Cash Flows

(Unaudited)

							Period From
							December 30, 1999
							(Date of Incorporation)
	Six Month Periods Ended June 30,						Through
	2008			2007			June 30, 2008
CASH FLOWS FROM OPERATING ACTIVITIES:
Net loss	$	(18,538,756	)	$	(15,069,521	)	$	(141,981,636	)
Adjustments to reconcile net loss to net cash used in operating activities:
Depreciation and amortization		1,310,785			837,162			6,316,638
Amortization of financing costs and accretion of debt discount		406,904			279,409			1,078,384
Loss from write-off of intangible assets		—			206,683			776,607
Loss from disposal of fixed assets		326,291			—			326,291
Share-based compensation		1,078,217			790,247			3,403,843
Change in fair value of preferred stock warrants		—			276,612			4,551,236
Change in fair value of convertible derivative liability		—			—			17,777,723
Changes in operating assets and liabilities:
Accounts receivable		(381,224	)		(54,984	)		(482,023	)
Inventories		(658,429	)		(304,060	)		(2,561,433	)
Other current assets		62,863			(220,015	)		(661,433	)
Other assets		—			10,830			(77,630	)
Accounts payable		(27,130	)		(368,282	)		1,963,588
Accrued and other current liabilities		(188,630	)		876,704			2,300,013
Net cash used in operating activities		(16,609,109	)		(12,739,215	)		(107,269,832	)
CASH FLOWS FROM INVESTING ACTIVITIES:
Proceeds from investment maturities		—			—			53,894
Purchases of property and equipment		(1,520,137	)		(1,122,347	)		(12,930,137	)
Investments in intangible assets		(161,472	)		(572,238	)		(5,794,287	)
Net cash used in investing activities		(1,681,609	)		(1,694,585	)		(18,670,530	)
CASH FLOWS FROM FINANCING ACTIVITIES:
Proceeds from related party notes payable		—			—			6,339,210
Proceeds from issuance of debt		—			12,500,000			12,500,000
Repayment of long term debt		(1,400,463	)		(142,736	)		(1,567,136	)
Payments on capital lease obligation		(17,952	)		(15,887	)		(77,167	)
Proceeds from principal and interest on CEO note receivable		—			64,942			1,530,740
Deferred financing fees		—			(114,565	)		(114,565	)
Proceeds from the issuance of common stock, net of offering expenses		(778,731	)		—			102,220,002
Proceeds from warrant redemptions		2,189			—			1,288,809
Proceeds from stock option exercises		163,310			—			283,335
Proceeds from the issuance of Series A convertible preferred stock		—			—			3,008,357
Proceeds from the issuance of Series B convertible preferred stock		—			—			5,375,001
Proceeds from the issuance of Series C convertible preferred stock		—			—			15,000,000
Proceeds from the issuance of Series C-2 convertible preferred stock		—			—			27,350,087
Proceeds from the issuance of Series D convertible preferred stock		—			—			46,793,897
Net cash provided by (used in) financing activities		(2,031,647	)		12,291,754			219,930,570
NET INCREASE (DECREASE) IN CASH AND CASH EQUIVALENTS		(20,322,365	)		(2,142,046	)		93,990,208
CASH AND CASH EQUIVALENTS — Beginning of period		114,312,573			29,112,429			—
CASH AND CASH EQUIVALENTS — End of period	$	93,990,208		$	26,970,383		$	93,990,208
NONCASH INVESTING AND FINANCING ACTIVITIES:
Equipment acquired under capital lease	$	—		$	—		$	117,685
Capital expenditures included in accounts payable		527,961			141,811			527,961
Patent costs capitalized and included in accounts payable					162,171			—
Patent and license costs capitalized and included in accrued liabilities		597,000			766,112			597,000
Common stock issued for note payable — related party								1,440,000
Payment of Series A convertible preferred stock dividends in additional shares of Series A convertible preferred stock								410,539
6% dividends earned on Series C-2 and Series D convertible preferred stock					3,180,329			9,889,878
Conversion of Preferred Stock, including accumulated dividends on Series C-2 and Series D convertible preferred stock into common stock								116,281,865
Conversion of convertible derivative liability to additional paid-in capital								47,554,882
Conversion of preferred stock warrant liability to warrants to acquire common stock								5,424,551
Conversion of related party notes payable and accrued interest of $204,335, into Series C-2 convertible preferred stock								7,212,156
Conversion of related party notes payable and accrued interest of $177,358 into Series D convertible preferred stock								6,516,568
Equity offering transaction costs included in other current assets and accrued liabilities					271,573

See notes to financial statements.

3

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements

As of June 30, 2008 and December 31, 2007, and

For the three and six month periods ended June 30, 2008 and 2007 and

for the Cumulative Period From December 30, 1999

(Date of Incorporation) through June 30, 2008

(Unaudited)

1. Description of Business

Nanosphere, Inc. (the “Company”) was incorporated in Delaware on December 30, 1999. On January 3, 2000, Nanosphere, Inc. merged with Nanosphere LLC, an entity owned by the founders of the Company. The Company began operations upon incorporation on December 30, 1999. The Company develops, manufactures and markets an advanced molecular diagnostics platform, the Verigene System, that enables simple, low cost, and highly sensitive genomic and protein testing on a single platform. The Company has been, since its inception, in the development stage, as defined by the Statement of Financial Accounting Standards (“SFAS”) No. 7, “Accounting and Reporting by Development Stage Enterprises.”

On October 16, 2007, each share of common stock, par value $0.01 per share, was automatically reclassified as and converted into 1/25 of a share of common stock, par value $0.01 per share, of the Company. All common stock share and per share amounts (except par value) have been adjusted to reflect the one-for-25 stock split. Additionally, in accordance with the Company’s Amended and Restated Certificate of Incorporation, as amended, the conversion ratio of the Company’s convertible preferred stock was also proportionately decreased.

On November 6, 2007, the Company closed on the sale of 8,050,000 shares related to its initial public offering (“IPO”) at $14.00 per share, less underwriting discounts and commissions. Upon the closing of the Company’s initial public offering, all of the Company’s outstanding convertible preferred stock converted into 13,048,119 shares of common stock. Net proceeds from the initial public offering were approximately $102 million, net of transaction expenses.

Basis of Presentation— The accompanying unaudited condensed financial statements of the Company have been prepared in accordance with accounting principles generally accepted in the United States of America and in conformity with Article 10 of Regulation S-X. Accordingly, they do not include all of the information and footnotes required by accounting principles generally accepted in the United States of America for complete financial statements. However, in the opinion of management, all adjustments, consisting only of normal recurring adjustments, unless otherwise noted herein, necessary to present fairly the results of operations, financial position and cash flows have been made. Therefore, these financial statements should be read in conjunction with the Company’s most recent audited financial statements for the year ended December 31, 2007 and notes thereto included in the Company’s Annual Report on Form 10-K. The results of operations for any interim period are not necessarily indicative of the results of operations expected for the full year.

The accompanying financial statements have been prepared on a going-concern basis, which contemplates the realization of assets and the satisfaction of liabilities in the normal course of business. The Company has incurred net losses attributable to common stock of $183.7 million since inception, and has funded those losses primarily through the sale and issuance of equity securities and secondarily through the issuance of debt and research and development contracts.

Use of Estimates— The preparation of financial statements in conformity with accounting principles generally accepted in the United States of America requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities and disclosure of contingent assets and liabilities at the date of the financial statements. Estimates also affect the reported amounts of expenses during the reporting period. The Company’s more significant estimates and assumptions include its accounting for stock-based compensation and the useful lives of its long lived assets. Actual results could differ from those estimates.

4

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

2. Net Loss Per Common Share

Basic and diluted net loss per common share have been calculated in accordance with SFAS No. 128, “Earnings Per Share”, for the three and six month periods ended June 30, 2008 and 2007. As the Company had a net loss in each of the periods presented, basic and diluted net loss per common share are the same.

The computations of diluted net loss per common share for the three and six month periods ended June 30, 2008 and 2007 did not include the effects of the following options to acquire common stock, convertible preferred stock, convertible preferred stock warrants and common stock warrants as the inclusion of these securities would have been antidilutive.

	Three and six month periods ended June 30,
	2008		2007
Stock options		3,368,919		3,173,548
Convertible preferred stock		—		12,291,401
Convertible preferred stock warrants		—		1,445,997
Common stock warrants		1,300,119		—
Total		4,669,038		16,910,946

3. Intangible Assets

Intangible assets, consisting of purchased intellectual property and capitalized patents costs, as of June 30, 2008 and December 31, 2007 comprise the following:

	June 30, 2008							December 31, 2007
	Carrying		Accumulated					Carrying		Accumulated
	Value		Amortization			Net		Value		Amortization			Net
Intellectual property —
Licenses	$	2,122,177	$	(258,852	)	$	1,863,325	$	1,870,178	$	(90,244	)	$	1,779,934
Patents		3,492,029		(264,674	)		3,227,355		3,370,323		(219,505	)		3,150,818
Total	$	5,614,206	$	(523,526	)	$	5,090,680	$	5,240,501	$	(309,749	)	$	4,930,752

Amortization expense for intangible assets amounted to $109,508 and $213,777 for the three and six month periods ended June 30, 2008 and to $49,657 and $63,818 for the three and six month periods ended June 30, 2007, respectively. Estimated future amortization expense is as follows:

Years Ending December 31
2008 (Period from July 1 to December 31)	$	225,019
2009		292,377
2010		222,377
2011		222,377
2012		222,377
Thereafter		1,279,624

Patent amortization commences on the grant date of the underlying patent and continues over the remaining life of the granted patent. Licenses are amortized from the date of the U.S. Food and Drug Administration (the “FDA”) clearance of products associated with the licensed technology and continues over the remaining life of the license. The future amortization expense reflected above is based on patents granted and licenses related to products cleared by the FDA as of June 30, 2008. The amortization period related to $0.5 million of licenses is not known as the diagnostic test products associated with the licensed technology have not been cleared by the FDA and, accordingly, amortization expense associated with the licenses is not included in the table above.

Costs deferred related to pending patent applications for which amortization has not commenced totaled $2.1 million as of June 30, 2008 and $2.0 million December 31, 2007. Costs deferred are written off if, and when, patent applications are abandoned or allowed to expire. No such write offs occurred during the three and six month periods ended June 30, 2008. Deferred patent costs written off in the three and six month periods ended June 30, 2007 were $206,683 in each period.

5

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

4. Related Party Transactions

Robert Letsinger and Chad Mirkin, co-founders of the Company, provide contracted research and development services to the Company, which are reimbursed based upon negotiated contract rates. The Company incurred expenses of $37,500 and $75,000 for these services in each of the three and six month periods, respectively, ended June 30, 2008 and June 30, 2007.

In March 2006, the Company issued to William Moffitt, the Company’s chief executive officer and a director, 320,000 shares of restricted common stock at a price of $4.50 per share, for an aggregate price of $1,440,000. In connection with this sale of common stock, the Company received a full recourse, long-term promissory note from William Moffitt for a total of $1,440,000. During 2007, $90,740 of interest was paid to the Company on the note and was recorded as additional paid in capital. In August 2007, the note receivable due from the Company’s chief executive officer was repaid.

5. Equity Incentive Plan

The Company’s board of directors has adopted and the shareholders have approved the Nanosphere 2000 Equity Incentive Plan and the Nanosphere 2007 Long-Term Incentive Plan. The plans authorize the compensation committee to grant stock options, share appreciation rights, restricted shares, restricted share units, unrestricted shares incentive stock options, deferred share units and performance awards. Option awards are generally granted with an exercise price equal to or above the fair value of the Company’s common stock at the date of grant with 10 year contractual terms. Certain employee options vest ratably over four years of service, while other employee options vest after seven years of service but provide for accelerated vesting contingent upon the achievement of various company-wide performance goals, such as decreasing time to market for new products and entering into corporate collaborations (as defined in the option grant agreements). For these “accelerated vesting” options, 20-25% of the granted option shares will vest upon the achievement of each of four or five milestones as defined in the option grant agreements, with any remaining unvested options vesting on the seven year anniversary of the option grant dates. Approximately 40% of the options granted and outstanding contain “accelerated vesting” provisions.

The fair values of the Company’s option awards granted during the six months ended June 30, 2008 were estimated at the date of grant using the Black-Scholes option pricing model with the following weighted-average assumptions:

Expected dividend yield		0	%
Expected volatility		70	%
Risk free interest rate		3.05	%
Weighted-average expected option life		6.44 years
Estimated weighted-average fair value
On the date of grant based on the above assumptions	$	7.32
Estimated forfeiture rate for unvested options		4.6	%

Expected volatility is based on calculated stock volatilities for publicly traded companies in the same industry and general stage of development as the Company. The risk-free rate is based on the U.S. Treasury yield curve in effect at the time of the grants for periods consistent with the expected life of the option. The expected life of options granted is derived from the average of the vesting period and the term of the option as defined in the Plans, following the guidance in SEC Staff Accounting Bulletins No. 107 and 110, as the Company has a limited history of employees exercising their stock options and therefore can not reasonably determine the expected life assumption based on its own historical data. Total compensation cost recognized was $548,505 and $1,078,217 in the three and six month periods ended June 30, 2008, respectively, and $744,921 and $790,247 for the three and six month periods ending June 30, 2007, respectively.

As of June 30, 2008, the total compensation cost not yet recognized related to the nonvested awards is approximately $8.2 million, which amount is expected to be recognized over the next four years, which is a weighted average term, without taking into account any potential acceleration of vesting that might occur as discussed above because the milestone events that would trigger acceleration are not yet deemed probable. While the Company does not have a formally established policy, as a practice the Company has delivered newly issued shares of its common stock upon the exercise of stock options.

6

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

A summary of option activity under the Plan as of June 30, 2008, and for the six month period then ended is presented below:

						Weighted
				Weighted		Average
				Average		Remaining		Aggregate
	Number of			Exercise		Contractual		Intrinsic
Options	Shares			Price		Term		Value of Options
Outstanding — January 1, 2008		3,141,530		$	4.97
Granted		328,000		$	11.21
Exercised		(36,291	)	$	4.50
Expired		(2,290	)	$	19.25
Forfeited		(62,030	)	$	4.50
Outstanding — June 30, 2008		3,368,919		$	5.59		8.41	$	9,811,514
Exercisable — June 30, 2008		744,017		$	6.11		7.64	$	2,142,487
Vested and Expected to Vest — June 30, 2008		3,248,174		$	5.59		8.40	$	9,458,739

There were no options exercised during the six month period ended June 30, 2007. The intrinsic value of options exercised during the three and six month period ended June 30, 2008 was $204,699.

Included in the number of options outstanding at June 30, 2008, are 1,337,210 options with a weighted average exercise price of $4.65 per share and accelerated vesting provisions based on the criteria mentioned above. None of the milestones which would accelerate vesting have occurred; therefore the related options are not exercisable as of June 30, 2008. The total fair value of shares vested during the three and six month periods ended June 30, 2008 was $1,078,616 and $1,110,376, respectively, and was $374,416 and $482,888 for the three and six month periods ended June 30, 2007, respectively.

6. License Agreements

In 2006, the Company entered into a license agreement with Northwestern University, which provides the Company with an exclusive license to certain patents and patent applications owned by Northwestern University that are related to nanotechnology and biobarcode technology. The Company has an obligation to pay Northwestern University a royalty at a rate that is a percentage of the gross profits of licensed products, subject to certain adjustments. The Company’s obligation for payments to Northwestern pursuant to this agreement began on January 1, 2007. No amounts were incurred under this obligation for the three and six month periods ended June 30, 2008 and 2007.

The Company has entered into several nonexclusive license agreements with various companies covering certain technologies which are embedded in the Company’s diagnostic instruments and diagnostic test products. As of June 30, 2008, the Company has paid aggregate initial license fees of $2,122,177 for these licenses, and has agreed to pay a percentage of net sales as royalties, in percentage amounts ranging from 1% to 12.0%. Certain of the license agreements have minimum annual royalty payments, and such minimum payments are $183,000 in 2008, $220,250 in 2009, $151,250 in 2010, $156,250 in 2011, $161,250 in 2012 and are $90,000 to $190,000 annually thereafter through the dates the respective licenses terminate. These licenses expire at various times, corresponding to the subject patents expirations, which currently range from 2009 to 2024.

7. Stockholders’ Equity

Convertible Preferred Stock

Prior to the completion of the Company’s IPO, the Company had outstanding four series of convertible preferred stock, two of which paid dividends and also had a conversion feature that the Company had determined was an embedded derivative instrument. The embedded derivative was bifurcated from the convertible preferred stock and accounted for separately as a liability, the convertible derivative liability. The conversion feature, when classified as a derivative liability, was required to be initially recorded at fair value and to be marked to fair value at the end of each reporting period, which resulted in a non-cash charge to other income or expense in the Statement of Operations. Upon the conversion of the Series C-2 and Series D convertible preferred stock to common stock during November 2007, the convertible derivative liability of $17.8 million was reclassified to Additional Paid-in Capital.

7

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

Prior to the completion of the Company’s IPO, the Company also issued warrants to purchase shares of convertible preferred stock in connection with certain of the convertible preferred stock financings. These warrants were accounted for as liabilities initially at fair value and were marked to fair value at the end of each reporting period up to the closing of the initial public offering, which resulted in a non-cash charge to other income or expense in the Statement of Operations.

With the exception of the Series D convertible preferred stock warrants, all convertible preferred stock warrants were either exercised or expired upon the completion of the IPO. The Series D convertible preferred stock warrants were converted to common stock warrants upon the closing of the IPO. As of June 30, 2008 and December 31, 2007, there were outstanding warrants to acquire shares of common stock of 1,300,119 and 1,300,319, respectively.

The expiration dates of the warrants outstanding at June 30, 2008 are as follows:

Series of Stock to	Number of		Expiration
which the Warrant is Exercisable	Warrants		Date
Common — exercise price of $13.13 per share		1,135,194	April 2011
Common — exercise price of $8.75 per share		164,925	April 2013

The exercise price on the common stock warrants with an exercise price of $13.13 per share at June 30, 2008, increases every April by $2.19 per share, until the exercise price equals $17.50 per share which will occur in April 2010.

8. Leases

The Company has an operating lease agreement expiring in 2010 for its office and laboratory space. The Company has also entered into a capital lease agreement expiring in 2009 for a piece of laboratory equipment. Rent expense was $169,142 and $336,696 for the three and six month periods ended June 30, 2008, respectively, and $165,242 and $339,267 for the three and six month periods ended June 30, 2007, respectively. The Company has a bargain purchase option of $11,563 on equipment under capital lease at the end of the lease term in 2009. The gross and net book values of the equipment acquired under the capital lease is $117,685 and $81,259, respectively, at June 30, 2008.

Annual future minimum obligations for operating and capital leases as of June 30, 2008, are as follows:

	Operating		Capital
Years Ending December 31	Leases		Leases
2008 (Period from July 1 to December 31)	$	254,790	$	21,090
2009		520,197		22,108
2010		220,898
Total minimum lease payments	$	995,885		43,198
Less amounts representing interest				(2,680	)
Net present value				40,518
Less current portion of net minimum lease payments				(40,518	)
Long-term portion of net minimum lease payments			$	—

9. Long Term Debt

In February 2007, the Company entered into two loan and security agreements, with commitments for debt financing with Venture Lending & Leasing IV, Inc., and Venture Lending & Leasing V, Inc. The Company borrowed $12.5 million under these agreements in February 2007. The Company paid interest, and a minimal amount of principal, for the initial twelve month period followed by a thirty month period within which the note principal will be amortized. Interest was paid during the initial twelve month period at a fixed annual interest rate of 12.5%, and during the following thirty month period will be paid at a fixed annual interest rate of 10.0%. Notes issued pursuant to this commitment are secured by a first security lien on all of the Company’s assets including intellectual property. In connection with the execution of the commitment and the initial note issuance under these agreements, the Company issued to the lenders 5,535,824 shares of the Company’s Series D convertible preferred stock.

The $12.5 million of proceeds received were allocated to debt and the Series D convertible preferred stock based on their fair values at the borrowing date with $1.9 million allocated to Series D convertible preferred stock and the remaining $10.6 million allocated to debt. The discount on the debt of $1.9 million results in an effective interest rate on the debt of 21% and the discount will be amortized to interest expense over the term of the debt following the interest method. Interest expense on this debt was $536,484 and $1,111,124 for the three and six month periods ended June 30, 2008, respectively, which includes $190,407 and $390,537 of discount amortization. Interest expense on this debt was $553,177 and $806,271 for the three and six month periods ended June 30, 2007, respectively, which includes $174,101 and $267,133 of discount amortization, respectively. The fair value of the debt at June 30, 2008 is $11.7 million, which was derived by applying an income approach to determine the present value of forecast debt payments as required under the debt agreements. To determine the appropriate yield by which to discount the cash flow payments, the Company relied upon observable inputs of yields for securities with comparable risk and return profiles, which are Level 2 measurements under SFAS No. 157, “Fair Value Measurements” (“SFAS No. 157”).

8

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

Aggregate annual principal payments on long-term debt are as follows:

2008 (July 1 to December 31)	$	2,197,359
2009		4,818,211
2010		3,917,293
Total	$	10,932,863

10. Inventory

Inventory on hand at June 30, 2008 and December 31, 2007 was comprised of the following:

	June 30, 2008		December 31, 2007
Work-in-process component parts	$	1,150,537	$	1,361,227
Finished goods		1,410,896		541,777
Total	$	2,561,433	$	1,903,004

11. Property and Equipment

Property and equipment was comprised of the following as of June 30, 2008 and December 31, 2007:

	June 30, 2008			December 31, 2007
Total property and equipment — at cost	$	12,949,062		$	11,729,127
Less accumulated depreciation		(5,492,107	)		(4,695,530	)
Net property and equipment	$	7,456,955		$	7,033,597

12. New Accounting Standards

Effective January 1, 2008, the Company adopted SFAS No. 157. SFAS No. 157 clarifies the definition of fair value, prescribes methods for measuring fair value, establishes a fair value hierarchy based on the inputs used to measure fair value and expands disclosures about the use of fair value measurements. In accordance with Financial Accounting Standards Board Staff Position No. FAS 157-2, “Effective Date of FASB Statement No. 157” (FSP 157-2), the Company will defer the adoption of SFAS No. 157 for our nonfinancial assets and nonfinancial liabilities, except those items recognized or disclosed at fair value on an annual or more frequently recurring basis, until January 1, 2009. The adoption of SFAS No. 157 did not have a material impact on our fair value measurements.

In February 2007, the FASB issued SFAS No. 159, “The Fair Value Option for Financial Assets and Financial Liabilities” (“SFAS No. 159”), SFAS No. 159 permits entities to choose to measure many financial instruments and certain other items at fair value. The objective is to improve financial reporting by providing entities with the opportunity to mitigate volatility in reported earnings caused by measuring related assets and liabilities differently without having to apply complex hedge accounting provisions. SFAS No. 159 was effective as of January 1, 2008 and had no impact on the Company’s financial position, results of operations, or cash flows as the Company has not elected to measure any assets or liabilities at fair value.

9

Nanosphere, Inc.
(A Development Stage Company)

Notes to Financial Statements — (Continued)
(Unaudited)

In December 2007, the FASB issued SFAS No. 141 (Revised 2007) “Business Combinations” (“SFAS 141(R)”). SFAS 141(R) is effective for business combinations that close on or after January 1, 2009, the first day of the Company’s annual reporting period beginning after December 15, 2008. SFAS 141(R) requires the recognition of assets acquired, liabilities assumed and any noncontrolling interest in the acquiree to be measured at fair value as of the acquisition date. Additionally, costs incurred to effect the acquisition are to be recognized separately from the acquisition and expensed as incurred. The provisions of SFAS No. 141(R) are to be applied prospectively. We do not expect that the adoption of SFAS 141(R) will materially impact the Company’s financial position, results of operations or cash flows.

Additionally, in December 2007, the FASB issued SFAS No. 160, “Noncontrolling Interests in Consolidated Financial Statements” (“SFAS 160”). SFAS 160 changes the reporting for minority interests by reporting these as noncontrolling interests within equity. Moreover, SFAS 160 requires that any transactions between an entity and a noncontrolling interest are to be accounted for as equity transactions. SFAS 160 is effective for financial statements issued for fiscal years beginning after December 15, 2008. SFAS 160 is to be applied prospectively, except for the presentation and disclosure requirements, which shall be applied retrospectively for all periods presented. Adoption of SFAS 160 will have no impact on the Company’s financial position, results of operations or cash flows.

In March 2008, the FASB issued SFAS No. 161, “Disclosures about Derivative Instruments and Hedging Activities — an amendment of FASB Statement No. 133” (“SFAS 161”). SFAS 161 is effective for reporting periods beginning after November 15, 2008. SFAS 161 amends and expands the disclosure requirements of FASB Statement No. 133 “Accounting for Derivative Instruments and Hedging Activities.” We do not expect that the adoption of SFAS 161 will materially impact the Company’s financial statement disclosures.

10

Item 2. Management’s Discussion and Analysis of Financial Condition and Results of Operations

This Quarterly Report onForm 10-Q contains forward-looking statements about us and our industry that involve substantial risks and uncertainties. All statements, other than statements of historical facts, included in this Quarterly Report onForm 10-Q regarding our strategy, future operations, future financial position, future net sales, projected expenses, prospects and plans and objectives of management are forward-looking statements. These statements involve known and unknown risks, uncertainties and other factors that may cause our actual results, levels of activity, performance or achievement to be materially different from those expressed or implied by the forward-looking statements.

In some cases, you can identify forward-looking statements by terms such as “anticipate,” “believe,” “estimate,” “expect,” “intend,” “may,” “might,” “plan,” “project,” “will,” “would,” “should,” “could,” “can,” “predict,” “potential,” “continue,” “objective,” or the negative of these terms, and similar expressions intended to identify forward-looking statements. However, not all forward-looking statements contain these identifying words. These forward-looking statements reflect our current views about future events and are based on assumptions and subject to risks and uncertainties. Given these uncertainties, you should not place undue reliance on these forward-looking statements. Actual events or results could differ materially from those expressed or implied by these forward-looking statements as a result of various factors.

These forward-looking statements represent our estimates and assumptions only as of the date of this Quarterly Report onForm 10-Q. Unless required by U.S. federal securities laws, we do not intend to update any of these forward-looking statements to reflect circumstances or events that occur after the statement is made or to conform these statements to actual results. The following discussion should be read in conjunction with the consolidated financial statements and notes thereto appearing elsewhere in this Quarterly Report onForm 10-Q. Our actual results may differ materially from those anticipated in these forward-looking statements as a result of various factors, including those set forth under “Risk Factors” and elsewhere in this Quarterly Report on Form 10-Q.

Business Overview

Nanosphere develops, manufactures and markets an advanced molecular diagnostics platform, the Verigene System, that enables simple, low cost and highly sensitive genomic and protein testing on a single platform. Our proprietary nanoparticle technology simplifies the ability to perform molecular diagnostic tests, achieves ultra-sensitive protein detection at limits beyond current diagnostic technologies, provides the ability to multiplex, or run multiple tests at the same time on the same sample, and enables the development of a broad menu of test assays to be performed on a single platform. We are currently developing diagnostic tests for a variety of medical conditions including cancer, neurodegenerative, cardiovascular and infectious diseases, as well as pharmaco-genomics, or tests for personalized medicine. There is a growing demand among laboratories to implement molecular diagnostic capabilities, but the cost and complexity of existing technologies and the need for specialized personnel and facilities have limited the number of laboratories with these capabilities. We believe that the Verigene System’s ease of use, rapid turnaround times, relatively low cost and ability to support a broad test menu will simplify work flow and reduce costs for laboratories already performing molecular diagnostic testing and allow a broader range of laboratories, including those operated by local hospitals, to perform molecular diagnostic testing. Our ability to detect proteins, which is at least 100 times more sensitive than current technologies, may enable earlier detection of and intervention in diseases associated with known biomarkers and the introduction of tests for new biomarkers that exist in concentrations too low to be detected by current technologies. We are focusing our efforts on the clinical diagnostics market and may seek opportunities either directly or through outlicensing arrangements to commercialize our technology in the industrial, biosecurity and other markets.

We received 510(k) clearance from the FDA for the Verigene System and our warfarin metabolism assay on September 17, 2007 and for our hyper-coagulation assay on October 12, 2007. We continue to develop diagnostic tests for a variety of medical conditions including human genetics, pharmacogenomics, infectious diseases and protein biomarkers in areas such as cancer and neurodegenerative and cardiovascular disease.

11

In November 2007, we completed our initial public offering of 8,050,000 shares of common stock at $14.00 per share. We received approximately $102 million of net proceeds from the offering.

Since our inception we have incurred net losses each year, and we expect to continue to incur losses for the foreseeable future. Our net loss attributable to common stock was $18.5 million for the six month period ended June 30, 2008. As of June 30, 2008, we had an accumulated deficit during the development stage of $183.7 million. Our operations to date have been funded principally through capital contributions from investors in our initial public offering of common stock, and prior thereto in our convertible preferred stock, which was converted to common stock in 2007, and our debt borrowings. We expect to incur increasing expenses over the next several years, principally to further develop our products and to develop additional diagnostic tests, as well as to further increase our spending to manufacture, sell and market our products.

Financial Operations Overview

Revenue

Product sales are derived from the sale or lease of the Verigene System, including cartridges and related products sold to research laboratories and hospitals. Revenue also consists of funds received under contracts and government grants, including funds for the reimbursement of certain research and development expenses. We expect future revenue growth to be driven by product sales instead of government grants and contracts.

Cost of Product Sales

Cost of product sales represents the cost of materials, direct labor, other overhead costs associated with manufacturing and delivering the Verigene System, including cartridges and related products and royalties on product sales.

Research and Development Expenses

Research and development expenses primarily include all expenses related to the development of the Verigene System and assays, and the research and development expenses associated with fulfilling our obligations to the United States government and under other contracts and grants. Such expenses include salaries and benefits for research and development personnel, contract services and other expenses. We expense all research and development costs in the periods in which they are incurred. We expect research and development expenses to increase as we work on the development and scale up of manufacturing methods, processes and procedures and the development of future generations of the Verigene System, and additional genomic and protein tests.

Sales, General and Administrative Expenses

Sales, general and administrative expenses principally include compensation for employees in our sales, customer service, marketing, management and administrative functions. We also include professional services, facilities, technology, communications and administrative expenses in sales, general and administrative. The professional services costs primarily consist of legal and accounting costs. We expect sales and marketing expenses to continue to increase in the future as a result of anticipated growth in our sales and customer support functions to support growth in our product sales. We expect general and administrative expenses to increase as a result of the need to hire additional administrative personnel and due to higher legal, accounting and related public company expenses.

Interest Income

Interest income principally includes interest earned on our excess cash balances. Such balances are primarily invested in money market and bank checking accounts at major financial institutions.

Interest Expense

Interest expense includes the interest charges related to our debt borrowings, including non-cash interest expense relating to the amortization of debt discount and issue costs.

12

Three Month Period Ended June 30, 2008 Compared to the Three Month Period Ended June 30, 2007

Revenues

Revenues were $0.2 million for the three months ended June 30, 2008, as compared to $0.5 million for the three months ended June 30, 2007. Product revenues increased to $0.2 million for the three months ended June 30, 2008 from $7,025 for the same period in 2007 due to sales and rentals of the Verigene System following clearance from the FDA during 2007. Revenues from contracts and government grants were zero for the three months ended June 30, 2008 and $0.5 million for the three months ended June 30, 2007.

Cost of Product Sales

For the three months ended June 30, 2008, cost of product sales was $0.2 million, as compared to $3,188 for the three months ended June 30, 2007. Cost of product sales increased during the three months ended June 30, 2008 due to the increase in Verigene System sales.

Research and Development Expenses

Research and development expenses increased to $6.0 million for the three months ended June 30, 2008, from $5.3 million for the three months ended June 30, 2007. This expense growth was a result of increased research and development personnel and contract services to develop and commercialize protein, human genetic and infectious diseases assays as well as our next generation testing platform. In addition, the increase in expenses resulted from development expenses incurred to scale up our manufacturing capabilities.

The $0.7 million increase in research and development expenses for the three months ended June 30, 2008 versus June 30, 2007 consists primarily of a $1.0 million increase in staffing and contract services, partially offset by a $0.3 million decrease in other research and development expenses.

Sales, General and Administrative Expenses

Sales, general and administrative expenses increased to $3.8 million for the three months ended June 30, 2008, from $3.1 million for the three months ended June 30, 2007. The $0.7 million increase was due primarily to a $0.3 million increase in sales personnel expenses and other marketing expenses to support the sales of the Verigene System and $0.4 million of public company related expenses including consulting, legal and audit fees.

Interest Expense

Interest expense was $0.5 million for the three months ended June 30, 2008, as compared to $0.6 million for the three months ended June 30, 2007. In February 2007, the Company borrowed $12.5 million under two loan and security agreements with Venture Lending & Leasing IV, Inc., and Venture Lending & Leasing V, Inc. The reduction in interest expense is driven by reduced principal outstanding.

Interest Income

Interest income was $0.5 million and $0.4 million for the three months ended June 30, 2008 and 2007, respectively. The increase in interest income in the three months ended June 30, 2008 resulted from the increase in cash from the initial public offering, which was partially offset by lower interest rates.

Six Month Period Ended June 30, 2008 Compared to the Six Month Period Ended June 30, 2007

Revenues

Revenues were $0.8 million in each of the six month periods ended June 30, 2008 and 2007. Product revenues increased by $0.4 million for the six months ended June 30, 2008 from the same period in 2007 due to Verigene System sales and rentals. Revenues from contracts and government grants were $0.3 million for the six months ended June 30, 2008 and $0.7 million for the six months ended June 30, 2007. We expect future revenue growth to be driven by product sales instead of government grants and contracts.

13

Cost of Product Sales

For the six months ended June 30, 2008, cost of product sales was $0.6 million, as compared to $18,367 for the six months ended June 30, 2007. Cost of product sales increased during the six months ended June 30, 2008 due to the increase in Verigene system sales.

Research and Development Expenses

Research and development expenses increased to $11.9 million for the six months ended June 30, 2008, from $10.2 million for the six months ended June 30, 2007. This expense growth was a result of increased research and development personnel and contract services to develop and commercialize protein, human genetic and infectious diseases assays as well as our next generation testing platform. In addition, the increase in expenses resulted from development expenses incurred to scale up our manufacturing capabilities.

The $1.7 million increase in research and development expenses for the six months ended June 30, 2008 versus June 30, 2007 consists primarily of $1.5 million in staffing and contract services and a $0.2 million increase in other expenses including facility expenses related to research and development.

Sales, General and Administrative Expenses

Sales, general and administrative expenses increased to $7.2 million for the six months ended June 30, 2008, from $5.3 million for the six months ended June 30, 2007. The $1.9 million increase was due primarily to $0.8 million of public company related expenses including consulting, legal and audit fees, a $0.6 million increase in sales personnel expenses and other marketing expenses to support the sales of the Verigene System and a $0.5 million increase in other sales, general and administrative expenses.

Interest Expense

Interest expense was $1.1 million for the six months ended June 30, 2008, as compared to $0.8 million for the six months ended June 30, 2007. In February 2007, the Company borrowed $12.5 million under two loan and security agreements with Venture Lending & Leasing IV, Inc., and Venture Lending & Leasing V, Inc. The increase in interest expense for the six months ended June 30, 2008 resulted from a full six-month period of outstanding debt in 2008 as compared to a partial period of borrowings during the six-month period in 2007.

Interest Income

Interest income was $1.5 million and $0.8 million for the six months ended June 30, 2008 and 2007, respectively. The increase in interest income in the six months ended June 30, 2008 resulted from the increase in cash from the initial public offering, which was partially offset by a reduction in interest rates.

Liquidity and Capital Resources

From our inception in December 1999 through June 30, 2008, we have received net proceeds of $102.2 million from our initial public offering, $103.9 million from the sale of convertible preferred stock and issuance of notes payable that were exchanged for convertible preferred stock, $12.5 million from our debt borrowings, and $9.1 million from grant and contract revenue. We have devoted substantially all of these funds to research and development and general and administrative expenses. Since our inception, we have had minimal revenues which have been derived from the sale of the Verigene System, including cartridges and related products, to research laboratories and pursuant to government contracts. Since inception, we have incurred significant losses and, as of June 30, 2008, we had an accumulated deficit during the development stage of approximately $183.7 million. We have not yet achieved profitability and anticipate that we will continue to incur net losses for the foreseeable future. We expect that our research and development, and sales, general and administrative expenses will continue to increase and, as a result, we will need to generate significant revenues to achieve profitability.

14

Our historical product sales have been minimal because our products, including the Verigene System, did not receive FDA clearance until September 17, 2007. On September 17, 2007, we received 510(k) clearance from the FDA for the Verigene System and our warfarin metabolism assay, and on October 12, 2007 we received FDA clearance for our hyper-coagulation assay. We have now commenced commercial sales of the Verigene System and these initial assays. Our expenses are likely to exceed our product sales for the next few years as we begin to market our FDA cleared products and continue to expand our FDA cleared tests. During this period we expect to spend approximately $50 million to finance ongoing research and development in connection with the development of additional tests and the next generation Verigene System and continued investments in and development of our product manufacturing infrastructure, including purchasing manufacturing equipment, tooling and facilities leasehold improvements, as well as increased manufacturing personnel. We also expect to spend approximately $40 million to fund additional sales, marketing and service personnel and marketing initiatives in connection with future test and system product launches. Positive cash flow from operations will depend upon revenue resulting from adoption of our initial products and expansion of our FDA cleared tests.

As of June 30, 2008, we had $94.0 million in cash and cash equivalents, compared to $114.3 million at December 31, 2007. The decrease of cash and cash equivalents was principally due to the use of $16.6 million in operating activities.

Net cash used in operating activities was $16.6 million for the six months ended June 30, 2008, compared to $12.7 million for the six months ended June 30, 2007. The increase in cash used was primarily due to the payment of approximately $1 million in bonuses and franchise taxes during the first quarter of 2008. In addition, the increase in cash used was due to an increase of sales, general and administrative expenses of $1.9 million and an increase in research and development expenses of $1.7 million.

Net cash used in investing activities was $1.7 million in each of the six-month periods ended June 30, 2008 and 2007. Spending on intangible assets decreased by $0.4 million, offset by an increase in investments in property and equipment of $0.4 million to scale up manufacturing of our FDA cleared products.

Net cash used in financing activities was $2.0 million for the six months ended June 30, 2008, compared to cash provided by financing activities of $12.3 million for the six months ended June 30, 2007. There were $1.4 million of payments on our long-term debt obligations during the six month period ended June 30, 2008. As previously discussed, in February 2007, we borrowed $12.5 million under two loan and security agreements which drove the cash from financing activities for the six month period ended June 30, 2007. In addition, approximately $0.8 million of transaction expenses related to our IPO were unpaid as of December 31, 2007. Such expenses were paid during the six month period ended June 30, 2008.

We anticipate that our current cash and cash equivalents will be sufficient to meet our currently estimated needs for at least the next two to three years. However, we may need additional financing to execute our current or future business strategies. We expect capital outlays and operating expenditures to increase over the next several years as we expand our infrastructure, commercialization, manufacturing, and research and development activities. The amount of additional capital we may need to raise depends on many factors, including:

• the level of research and development investment required to maintain and improve our technology;

• the amount and growth rate of our revenues;

• changes in product development plans needed to address any difficulties in manufacturing or commercializing the Verigene System and enhancements to our system;

• the costs of filing, prosecuting, defending and enforcing patent claims and other intellectual property rights;

• competing technological and market developments;

• our need or decision to acquire or license complementary technologies or acquire complementary businesses; and

• changes in regulatory policies or laws that affect our operations.

15

We cannot be certain that additional capital will be available when and as needed or that our actual cash requirements will not be greater than anticipated. If we require additional capital at a time when investment in diagnostics companies or in the marketplace in general is limited due to the then prevailing market or other conditions, we may not be able to raise such funds at the time that we desire or any time thereafter. In addition, if we raise additional funds through the issuance of equity or convertible debt securities, the percentage ownership of our stockholders could be significantly diluted, and these newly-issued securities may have rights, preferences or privileges senior to those of existing stockholders. If we obtain additional debt financing, a substantial portion of our operating cash flow may be dedicated to the payment of principal and interest on such indebtedness, and the terms of the debt securities issued could impose significant restrictions on our operations. If we raise additional funds through collaborations and licensing arrangements, we might be required to relinquish significant rights to our technologies or products, or grant licenses on terms that are not favorable to us.

Contractual Obligations and Commitments

Other than disclosed elsewhere in this Quarterly Report, our commitments have not changed significantly from disclosures included in our Annual Report on Form 10-K for the fiscal year ended December 31, 2007 filed with the SEC on March 21, 2008.

Off-Balance Sheet Arrangements

We do not have any off-balance sheet financing or unconsolidated special-purpose entities.

Recent Accounting Pronouncements

See Note 12 to the Financial Statements for a discussion of recent accounting pronouncements.

Item 3. Quantitative and Qualitative Disclosures About Market Risk

Our exposure to market risk is currently confined to our cash and cash equivalents. We have not used derivative financial instruments for speculation or trading purposes. The primary objective of our investment activities is to preserve our capital for the purpose of funding operations while at the same time maximizing the income we receive from our investments without significantly increasing risk. To achieve these objectives, our investment policy allows us to maintain a portfolio of cash equivalents and short-term investments through a variety of securities, including commercial paper, money market funds and corporate debt securities. Our cash and cash equivalents through June 30, 2008 included amounts in bank checking and liquid money market accounts. As a result, we believe we have minimal interest rate risk; however, a one percentage point decrease in the average interest rate on our portfolio would have reduced interest income for the six month period ended June 30, 2008 by $509,889.

Item 4. Controls and Procedures

(a) Evaluation of Disclosure Controls and Procedures

Management of the Company, with the participation of the Chief Executive Officer and the Chief Financial Officer, evaluated the effectiveness of the design and operation of the Company’s disclosure controls and procedures (as defined in Rules 13a-15(e) and 15d-15(e) of the Securities Exchange Act of 1934, as amended) as of June 30, 2008. The Company’s disclosure controls and procedures are designed to ensure that information required to be disclosed by the Company in the reports it files or submits under the Exchange Act is recorded, processed, summarized and reported on a timely basis and that such information is accumulated and communicated to management, including the Chief Executive Officer and Chief Financial Officer, as appropriate, to allow timely decisions regarding disclosure. Based upon this evaluation, the Chief Executive Officer and the Chief Financial Officer have concluded that the Company’s disclosure controls and procedures were effective as of June 30, 2008.

(b) Changes in Internal Control over Financial Reporting

There have been no material changes to the Company’s internal control over financial reporting during the quarterly period ended June 30, 2008.

16

PART II.
OTHER INFORMATION

Item 1. Legal Proceedings

We are from time to time subject to various claims and legal actions during the ordinary course of our business. We believe that there are currently no claims or legal actions that would, in management’s judgment based on information currently available, have a material adverse effect on our results of operations, cash flows or financial condition.

Item 1A. Risk Factors

In addition to the other information set forth in this report, you should carefully consider the factors discussed in “Risk Factors” in Part I, Item 1A of our Annual Report on Form 10-K for the year ended December 31, 2007, which could materially affect our business, cash flows, financial condition or future results. There have been no material changes from the risk factors previously disclosed in our Report on Form 10-K.

Item 2. Unregistered Sales of Equity Securities and Use of Proceeds

During the three months ended June 30, 2008, there were no sales of unregistered securities.

Item 4. Submission of Matters to a Vote of Security Holders

Our Annual Meeting of Shareholders was held on May 29, 2008 in Chicago, Illinois. The following matters were presented to our stockholders: (1) Proposal No. 1 — election of seven directors to serve until our next annual meeting of stockholders in 2009, and (2) Proposal No. 2 — ratification of the audit committee’s selection of Deloitte & Touche LLP as the Company’s independent registered public accounting firm for the fiscal year ending December 31, 2008. The results of the votes are as follows:

Proposal 1 — Election of directors by stockholders

	For		Withheld
William P. Moffitt III		17,874,059		1,939,184
Mark Slezak		19,775,347		37,896
Jeffrey R. Crisan		17,572,519		2,240,724
Andre de Bruin		19,775,542		37,701
Chad A. Mirkin		17,862,253		1,950,990
James J. Nahirny		17,571,946		2,241,297
Sheli Z. Rosenberg		19,775,542		37,701

Proposal 2 — Ratification of Deloitte & Touche LLP as independent registered public accounting firm

	For
For		19,796,687
Against		14,206
Abstain		2,350

17

Item 6. Exhibits, Financial Statement Schedules

Exhibit Number		Exhibit Description
	31.1	Certification of Chief Executive Officer pursuant to Exchange Act Rules 13a-14(a) and 15d-14(a), as adopted pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 *
	31.2	Certification of Chief Financial Officer pursuant to Exchange Act Rules 13a-14(a) and 15d-14(a), as adopted pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 *
	32.1	Certification of Chief Executive Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 *
	32.2	Certification of Chief Financial Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 *

* Filed herewith

18

SIGNATURES

Pursuant to the requirements of Section 13 or 15(d) of the Securities Exchange Act of 1934, the registrant has duly caused this report to be signed on its behalf by the undersigned, thereunto duly authorized.

	NANOSPHERE, INC.
	By:	/s/ William P. Moffitt
		William P. Moffitt
		President and Chief Executive Officer
Date: August 14, 2008
	By:	/s/ Roger Moody
		Roger Moody
		Chief Financial Officer and Treasurer
Date: August 14, 2008

19

EXHIBIT INDEX

Exhibit Number		Exhibit Description
	31.1	Certification of Chief Executive Officer pursuant to Exchange Act Rules 13a-14(a) and 15d-14(a), as adopted pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 *
	31.2	Certification of Chief Financial Officer pursuant to Exchange Act Rules 13a-14(a) and 15d-14(a), as adopted pursuant to Section 302 of the Sarbanes-Oxley Act of 2002 *
	32.1	Certification of Chief Executive Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 *
	32.2	Certification of Chief Financial Officer pursuant to 18 U.S.C. Section 1350, as adopted pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 *

20