Globalscape 8K Regulation FD Disclosure | GSB 27 Oct 16

Exhibit 99.1

Safe Harbor Statement * © 2016 Globalscape This presentation contains forward-looking statements within the meaning of Section 27A of the Securities Act of 1933 and Section 21E of the Securities Exchange Act of 1934. The words "would," "exceed," "should," "anticipates," "believe," "steady," "dramatic," “expect,” and variations of such words and similar expressions identify forward-looking statements, but their absence does not mean that a statement is not a forward-looking statement. These forward-looking statements are based upon the Company’s current expectations and are subject to a number of risks, uncertainties and assumptions. The Company undertakes no obligation to update any forward-looking statements, whether as a result of new information, future events or otherwise. Among the important factors that could cause actual results to differ significantly from those expressed or implied by such forward-looking statements are risks that are detailed in the Company’s Annual Report on Form 10-K for the 2015 fiscal year, filed with the Securities and Exchange Commission on March 3, 2016.

Who is Globalscape * © 2016 Globalscape Globalscape is a managed file transfer (MFT) software company that secures mission-critical exchanges of data across multiple platforms for individuals, enterprises and governments across a wide range of industries worldwide Core product: Enhanced File Transfer (EFT) platform EFT allows for the secure exchange of business information, anywhere, anytime with military-proven security and is an affordable, customizable platformEFT helps organizations secure and protect their sensitive data from unauthorized use Trades on NYSE MKT under ticker GSBMarket Capitalization approximately $72 millionExceptional customer retention rate, 90+%13,000 customers in over 150 countries Headquartered in San Antonio, TX, with approximately 125 employees

Leadership Profile * © 2016 Globalscape Company leadership team are experts in their chosen fields On average the team has 20 years of experience in the technology and software industries, specifically security and networking Full leadership team is committed to transparency and visibility Role they play executing company strategy is key to our success

* © 2016 Globalscape What is Managed File Transfer? Automated transfer between two servers with no (error-prone) human interaction Server-to-Server File Transfer A software application sends a file to a server or an email address, or sends a notification that a file is available for pickup Server-to-Person File Transfer Users send files to each other through email, such as Outlook, or an online portal Person-to-Person File Transfer A user sends a file to a server, such as a shared drive in your office, where it is available to your coworkers or the server itself Person-to-Server File Transfer

* © 2016 Globalscape Why Do Customers Purchase MFT? My organization needs the ability to scale painlessly. I want to send big files. I need visibility and control over my data. My team needs a secure solution that can work with our current set of tools. My team needs a solution with experienced service and support My department needs to comply with industry and government regulations. I don't want to be a statistic. I spend all my time waiting for files.

* © 2016 Globalscape Healthcare—Research Data, Clinical Trials, Patient Records, Scanned Images, X-Rays, Test Results Retail—Information, Purchase Orders, Point of Sale Data, Product Images, Catalogs, Trend Analysis Info, Customer Info Financial Services—Digital Check Images, Payroll Information, Claim Information, Transaction Data, Account Statements Business Services—Human Resources, Billing Information, Partner Interaction Industries That Need Managed File Transfer Manufacturing—Manufacturing Files, Design Specs, Simulation Models, Scheduling Data Inventories Technology—Error Reports, Secure Remote Backup, Big Data, Digital Asset Distribution Entertainment & Media—Video Files, Soundtrack Files, 3D Animation, High Resolution Images Government—Personnel Records, Logistics Data, Maintenance Data, Legal Reference, Financial Records, Secure Files

What We Do * © 2016 Globalscape OPERATIONAL EFFICIENCY VISIBILITY AND REPORTING AUTOMATED DATA TRANSFER EASILY INTEGRATE LEGACY SYSTEMS MULTI-LAYERED SECURITY CLOUD/ VIRTUALIZED ENVIRONMENT-CAPABLE SHARE FILES VIA MOBILE DEVICES OR THE WEB MODULAR DESIGN CENTRALIZED MANAGEMENT IDENTITYAND ACCESS MANAGEMENT A ROBUST PLATFORM DESIGNED TO SECURELY TRANSFER DATA COMPLIANCE GOVERNANCE

* © 2016 Globalscape Industry pioneer with longevity in the marketplace Specialist in the Managed File Transfer market Relentless focus on customers, supported by retention and support rates Technology is easy to set up, manage and use – out of the box Key Corporate Differentiators

Globalscape Financial Position * © 2016 Globalscape Strong Balance Sheet (reported as of 9/30/2016)$20.7 million in Cash and Investments $0.0 notes payable and long-term debt Revenue for the first nine months of 2016 of $24.4 million, up 9% YoY Q3 2016 revenue was $8.8 million, a 15% increase compared to Q3 2015 Adjusted EBITDA for Q3 2016 was $2.3 million, an 18% percent increase compared to Q3 2015 Q3 2016 Net Income was $1.4 million, a 14% percent increase compared to Q3 2015 Basic earnings per share were $0.07 for Q3 2016 versus $0.06 for Q3 2015

2016-2017 Roadmap © 2016 Globalscape *Roadmap subject to change Synchronization, Sharing & Collaboration Workspaces 2.0Updated compatibility with MS OutlookIntegration of Mail Express sharing capabilities into MFT platformDMZ gateway support for sharing and synchronization Synchronization of shared data and support for external devicesSecure channel for WAN synchronization New SMB ModulesAccelerationscInsightAdvanced Authentication module Cloud Connectors module Distributed ClusteringETL module (ETLM) Managed File Transfer Web portal for secure file pick up and deliveryPolicy driven sharingIntegration of scConnect capabilities into EFTEnhancement of collaboration features Scalibility improvementsBranch Office Edition (BOE)Unified Compliance moduleData Security Module Improved management & integration capabilities (Web admin, API) FY16 FY17 12

Multiple Licensing and Service Revenue Paths © 2016 Globalscape EFT CLOUD SERVICES EFT Cloud Services offers enterprise-level managed file transfer capabilities as SaaS MFT solution, delivered through the cloud with top hosting providers HYBRID CLOUDThe combination of EFT on-premises and EFT Cloud Services provides the security, management, and monitoring of EFT and the 24x365 access that employees, customers, and partners demand ON-PREMISESEnhanced File Transfer (EFT) is our proprietary managed file transfer platform, providing:Security & ComplianceAutomationControl & VisibilityProgrammatic API ControlHigh Availability Active-Active Clustering *

Meet the customer where they want to be: Globalscape’s Cloud, Popular Infrastructure as a Service platforms (AWS, Azure) or Hybrid Globalscape Cloud Strategy * © 2016 Globalscape Platform or Fully Managed Service offering: Globalscape and its partners will manage your EFT for you Flexible licensing: Bring your Own License (aka traditional perpetual), monthly subscription, fully metered use-based

World Class Go to Market Strategy © 2016 Globalscape Multiple Customer Facing Sales Teams Channel Facing Sales Team Dedicated Sales Engineering Resources Business Development Resources Demand GenerationSignificantly Increases Reach *

Globalscape Investment Highlights * © 2016 Globalscape Consistent top-line growthHighly recurring revenue streamExceptional customer retention rate at 90+%Q3 2016 marked 16 consecutive quarters of profitability$20.7M cash reservesNo debt Revenue

* © 2016 Globalscape Where We’re Going Refocusing on core technology, EFT, allowing us to bring new features to market quickly, helping to increase customer satisfaction, and address new opportunities where we can capitalize on demand and sales Expecting organic growth as a result of new or enhanced technology alliance partnerships or potential technology acquisitionsUsing cash to make strategic investments in adding to our core technologies with proven revenue streams, while managing them to higher profitabilitySeeing long-term investments in the channel and demand generation programs paying off

Globalscape (GSB) 8-KRegulation FD Disclosure