Exhibit 31.1
CERTIFICATION OF CHIEF EXECUTIVE OFFICER PURSUANT TO SECTION 302 OF THE SARBANES-OXLEY ACT OF 2002
I, Michael A. Martino, certify that:
1. I have reviewed this Annual Report on Form 10-K/A (Amendment No. 1) for the fiscal year ended December 31, 2013 of Ambit Biosciences Corporation; and
2. Based on my knowledge, this report does not contain any untrue statement of a material fact or omit to state a material fact necessary to make the statements made, in light of the circumstances under which such statements were made, not misleading with respect to the period covered by this report.
| /s/ MICHAEL A. MARTINO |
| Michael A. Martino |
| President and Chief Executive Officer |
Date: August 8, 2014