Exhibit 21.1
LIST OF SUBSIDIARIES
The following is a list of the direct and indirect subsidiaries of Novo Integrated Sciences, Inc. as of September 12, 2022.
| Company | Jurisdiction of Incorporation or Organization | |
| Novo Healthnet Limited (1) | Ontario, Canada | |
| Novomerica Health Group, Inc. (1) | Nevada | |
| PRO-DIP, LLC (1) | New York | |
| Acenzia, Inc. (2) | Ontario, Canada | |
| Terragenx Inc. (3) | Canada | |
| Clinical Consultants International LLC (1) | Delaware | |
| Novo Healthnet Rehab Limited (1) | Ontario, Canada | |
| Novo Assessments Inc. (1) | Ontario, Canada | |
| Novo Healthnet Kemptville Centre, Inc. (4) | Ontario, Canada | |
| 12858461 Canada Corp. (5) | Canada |
| (1) | 100% owned subsidiary of Novo Integrated Sciences, Inc. |
| (2) | 100% owned subsidiary of Novo Healthnet Limited |
| (3) | 91% owned subsidiary by Novo Healthnet Limited |
| (4) | 80% owned subsidiary of Novo Healthnet Limited |
| (5) | 50.1% owned by Novo Healthnet Limited |
