Applied Biotech POSASR Automatic shelf registration (post-effective amendment)

Exhibit 12.1

Calculation of Ratios of Earnings to Fixed Charges
(in thousands)

	Three Months Ended March 31,					Year Ended December 31,
	2009		2008			2008			2007			2006			2005			2004
RATIO OF EARNINGS TO FIXED CHARGES:
Pre-tax (loss) income from continuing operations before adjustment for income from equity investee		8,404		(5,583	)		(38,132	)		(248,767	)		(11,750	)		(12,390	)		(14,321	)
Fixed charges:
Interest expense (a)		17,871		25,651			101,144			83,025			26,570			21,795			22,114
Interest portion of rent expense (b)		2,183		1,798			8,845			4,351			2,956			2,504			1,849
Total fixed charges		20,054		27,449			109,989			87,376			29,526			24,299			23,963
Total earnings (loss)		28,458		21,866			71,857			(161,391	)		17,776			11,909			9,642
Ratio of earnings to fixed charges		1.4x		0.8x			0.7x			—			0.6x			0.5x			0.4x
Ratio of coverage deficiency				(c	)		(c	)		(c	)		(c	)		(c	)		(c	)

(a)	Interest expense includes the write-off and amortization of deferred financing costs and the write-off and amortization of non-cash discounts associated with our debt issuances.
(b)	Includes approximately one-fourth of the rent expense for each period presented which management believes is a reasonable approximation of the interest component of such rentals.
(c)	For the three months ended March 31, 2008 and the years ended December 31, 2008, 2007, 2006, 2005 and 2004, our earning were insufficient to fully cover our fixed charges. The amount of the coverage deficiency in such periods was $5.6 million, $38.1 million, $248.8 million, $11.8 million, $12.4 million and $14.3 million, respectively.