HTG Molecular Diagnostics (HTGMQ) 10-Q 11 Aug 20 2020 Q2 Quarterly report Financial data

Document and Entity Information

Document and Entity Information - shares	6 Months Ended
	Jun. 30, 2020	Aug. 07, 2020
Cover [Abstract]
Document Type	10-Q
Amendment Flag	false
Document Period End Date	Jun. 30, 2020
Document Fiscal Year Focus	2020
Document Fiscal Period Focus	Q2
Trading Symbol	HTGM
Entity Registrant Name	HTG Molecular Diagnostics, Inc
Entity Central Index Key	0001169987
Entity Current Reporting Status	Yes
Current Fiscal Year End Date	--12-31
Entity Filer Category	Non-accelerated Filer
Entity Shell Company	false
Entity Small Business	true
Entity Emerging Growth Company	true
Entity Ex Transition Period	true
Entity Common Stock, Shares Outstanding		70,681,737
Entity File Number	001-37369
Entity Incorporation, State or Country Code	DE
Entity Tax Identification Number	86-0912294
Entity Address, Address Line One	3430 E. Global Loop
Entity Address, City or Town	Tucson
Entity Address, State or Province	AZ
Entity Address, Postal Zip Code	85706
City Area Code	(877)
Local Phone Number	289-2615
Document Quarterly Report	true
Document Transition Report	false
Entity Interactive Data Current	Yes
Title of 12(b) Security	Common Stock, $0.001 par value per share
Security Exchange Name	NASDAQ

Condensed Consolidated Balance

Condensed Consolidated Balance Sheets - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Current assets:
Cash and cash equivalents	$ 21,014,375	$ 7,619,748
Short-term investments available-for-sale, at fair value	11,852,173	25,410,222
Restricted cash		3,270,247
Accounts receivable	1,011,462	3,164,176
Inventory, net of allowance of $29,587 at June 30, 2020 and $39,403 at December 31, 2019	1,388,570	1,269,667
Prepaid expenses and other	1,236,192	633,522
Total current assets	36,502,772	41,367,582
Operating lease right-of-use assets	905,459	1,209,145
Property and equipment, net	1,825,191	2,240,133
Other non-current assets	69,365	302,409
Total assets	39,302,787	45,119,269
Current liabilities:
Accounts payable	927,829	1,662,583
Accrued liabilities	947,382	1,870,296
Contract liabilities - current	418,874	426,014
NuvoGen obligation - current	863,611	1,152,233
Short-term debt, net	1,143,011	2,987,667
Operating lease liabilities - current	584,073	758,932
Other current liabilities	30,983	41,134
Total current liabilities	4,915,763	8,898,859
NuvoGen obligation - non-current, net of discount	4,359,218	4,498,777
Long-term debt, net	10,705,219	6,871,545
Operating lease liabilities - non-current	453,404	636,340
Other non-current liabilities	185,276	244,114
Total liabilities	20,618,880	21,149,635
Commitments and Contingencies (Note 15)
Stockholders’ equity:
Series A convertible preferred stock, $0.001 par value; 51,270 shares authorized at June 30, 2020 and no shares authorized at December 31, 2019; 23,770 shares issued and outstanding at June 30, 2020 and no shares issued and outstanding at December 31, 2019	24
Common stock, $0.001 par value; 200,000,000 shares authorized at June 30, 2020 and December 31, 2019, 67,093,683 shares issued and outstanding at June 30, 2020 and 58,090,233 shares issued and outstanding at December 31, 2019	67,093	58,090
Additional paid-in-capital	200,079,879	194,234,151
Accumulated other comprehensive income (loss)	23,003	(4,964)
Accumulated deficit	(181,486,092)	(170,317,643)
Total stockholders’ equity	18,683,907	23,969,634
Total liabilities and stockholders' equity	$ 39,302,787	$ 45,119,269

Condensed Consolidated Balanc_2

Condensed Consolidated Balance Sheets (Parenthetical) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Inventory net allowance	$ 29,587	$ 39,403
Common stock, par value	$ 0.001	$ 0.001
Common stock, shares authorized	200,000,000	200,000,000
Common stock, shares issued	67,093,683	58,090,233
Common stock, shares outstanding	67,093,683	58,090,233
Series A Convertible Preferred Stock
Preferred stock, par value	$ 0.001	$ 0.001
Preferred stock, shares authorized	51,270	0
Preferred stock, shares issued	23,770	0
Preferred stock, shares outstanding	23,770	0

Condensed Consolidated Statemen

Condensed Consolidated Statements of Operations - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Revenue:
Total revenue	$ 1,963,294	$ 5,796,320	$ 4,188,768	$ 8,999,145
Operating expenses:
Cost of product and product-related services revenue	$ 974,642	$ 2,508,371	$ 1,990,134	$ 4,553,898
Type of Cost, Good or Service [Extensible List]	htgm:ProductAndProductRelatedServicesMember	htgm:ProductAndProductRelatedServicesMember	htgm:ProductAndProductRelatedServicesMember	htgm:ProductAndProductRelatedServicesMember
Selling, general and administrative	$ 4,255,485	$ 4,740,710	$ 8,930,748	$ 9,141,576
Research and development	1,732,776	3,253,639	3,659,051	5,328,387
Total operating expenses	6,962,903	10,502,720	14,579,933	19,023,861
Operating loss	(4,999,609)	(4,706,400)	(10,391,165)	(10,024,716)
Other income (expense):
Interest expense	(250,759)	(238,796)	(476,089)	(472,763)
Interest income	68,002	154,774	209,898	336,211
Other income			22,268
Loss on extinguishment of MidCap Credit Facility and QNAH Convertible Note	(522,394)		(522,394)
Total other expense	(705,151)	(84,022)	(766,317)	(136,552)
Net loss before income taxes	(5,704,760)	(4,790,422)	(11,157,482)	(10,161,268)
Provision for income taxes	(5,791)	(6,848)	(10,967)	(6,848)
Net loss	$ (5,710,551)	$ (4,797,270)	$ (11,168,449)	$ (10,168,116)
Net loss per share, basic and diluted	$ (0.09)	$ (0.17)	$ (0.17)	$ (0.36)
Shares used in computing net loss per share, basic and diluted	65,757,458	28,657,384	65,162,695	28,629,189
Product and Product-related Services
Revenue:
Total revenue	$ 1,728,526	$ 4,424,368	$ 3,716,663	$ 7,086,873
Collaborative Development Services
Revenue:
Total revenue	$ 234,768	$ 1,371,952	$ 472,105	$ 1,912,272

Condensed Consolidated Statem_2

Condensed Consolidated Statements of Comprehensive Loss - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Statement Of Income And Comprehensive Income [Abstract]
Net loss	$ (5,710,551)	$ (4,797,270)	$ (11,168,449)	$ (10,168,116)
Other comprehensive income (loss), net of tax effect:
Unrealized gain (loss) on short-term investments	(26,136)	819	21,561	4,172
Foreign currency translation adjustment	909	(9,194)	6,406	(2,755)
Total other comprehensive income (loss)	(25,227)	(8,375)	27,967	1,417
Comprehensive loss	$ (5,735,778)	$ (4,805,645)	$ (11,140,482)	$ (10,166,699)

Condensed Consolidated Statem_3

Condensed Consolidated Statements of Changes in Stockholders' Equity - USD ($)	Total	LP Purchase Agreement	ATM Offering	Private Placement	Series A Convertible Preferred Stock	Series A Convertible Preferred StockPrivate Placement	Common Stock	Common StockLP Purchase Agreement	Common StockATM Offering	Additional Paid-In Capital	Additional Paid-In CapitalLP Purchase Agreement	Additional Paid-In CapitalATM Offering	Additional Paid-In CapitalPrivate Placement	Accumulated Other Comprehensive Income (Loss)	Accumulated Deficit
Balance at Dec. 31, 2018	$ 21,092,062						$ 28,585			$ 172,086,909				$ (3,453)	$ (151,019,979)
Balance, shares at Dec. 31, 2018							28,585,449
Exercise of stock options	51,570						$ 22			51,548
Exercise of stock options, shares							21,871
Stock-based compensation expense	241,233									241,233
Release of restricted stock awards	13						$ 13
Release of restricted stock awards, shares							13,126
Net share settlement of restricted stock award	(9,685)						$ (4)			(9,681)
Net share settlement of restricted stock award, shares							(3,813)
Employee stock purchase plan expense	16,794									16,794
Net loss	(5,370,846)														(5,370,846)
Unrealized gain (loss) on short-term investments	3,353													3,353
Foreign currency translation adjustment	6,439													6,439
Balance at Mar. 31, 2019	16,030,933						$ 28,616			172,386,803				6,339	(156,390,825)
Balance, shares at Mar. 31, 2019							28,616,633
Balance at Dec. 31, 2018	21,092,062						$ 28,585			172,086,909				(3,453)	(151,019,979)
Balance, shares at Dec. 31, 2018							28,585,449
Net loss	(10,168,116)
Unrealized gain (loss) on short-term investments	4,172
Balance at Jun. 30, 2019	11,669,992						$ 28,707			172,831,416				(2,036)	(161,188,095)
Balance, shares at Jun. 30, 2019							28,707,047
Balance at Mar. 31, 2019	16,030,933						$ 28,616			172,386,803				6,339	(156,390,825)
Balance, shares at Mar. 31, 2019							28,616,633
Exercise of stock options	69,384						$ 32			69,352
Exercise of stock options, shares							32,385
Stock-based compensation expense	287,317									287,317
Release of restricted stock awards	15						$ 15
Release of restricted stock awards, shares							15,001
Net share settlement of restricted stock award	(11,152)						$ (4)			(11,148)
Net share settlement of restricted stock award, shares							(4,461)
Stock issued under stock purchase plans	99,140						$ 48			99,092
Stock issued under stock purchase plans, shares							47,489
Net loss	(4,797,270)														(4,797,270)
Unrealized gain (loss) on short-term investments	819													819
Foreign currency translation adjustment	(9,194)													(9,194)
Balance at Jun. 30, 2019	11,669,992						$ 28,707			172,831,416				(2,036)	(161,188,095)
Balance, shares at Jun. 30, 2019							28,707,047
Balance at Dec. 31, 2019	23,969,634						$ 58,090			194,234,151				(4,964)	(170,317,643)
Balance, shares at Dec. 31, 2019							58,090,233
Stock-based compensation expense	415,256									415,256
Release of restricted stock awards	36						$ 36
Release of restricted stock awards, shares							35,942
Net share settlement of restricted stock award	(7,052)						$ (11)			(7,041)
Net share settlement of restricted stock award, shares							(11,266)
Employee stock purchase plan expense	5,379									5,379
Issuance of common stock			$ 2,435,912					$ 615	$ 4,399		$ (615)	$ 2,431,513
Issuance of common stock, shares								615,384	4,399,062
Issuance of preferred stock	2,424,900			$ 562,955	$ 41	$ 10				2,424,859			$ 562,945
Issuance of preferred stock, shares					41,100	10,170
Cancellation of common stock received in exchange for Series A convertible preferred stock	(2,424,900)						$ (4,110)			(2,420,790)
Cancellation of common stock received in exchange for Series A convertible preferred stock, shares							(4,110,000)
Exercise of pre-funded warrants	31,768						$ 3,177			28,591
Exercise of pre-funded warrants, shares							3,176,762
Adjustment in connection with LP Purchase Agreement commitment fee and transaction costs		$ (96,000)									(96,000)
Net loss	(5,457,898)														(5,457,898)
Unrealized gain (loss) on short-term investments	47,697													47,697
Foreign currency translation adjustment	5,497													5,497
Balance at Mar. 31, 2020	21,913,184				$ 51		$ 62,196			197,578,248				48,230	(175,775,541)
Balance, shares at Mar. 31, 2020					51,270		62,196,117
Balance at Dec. 31, 2019	23,969,634						$ 58,090			194,234,151				(4,964)	(170,317,643)
Balance, shares at Dec. 31, 2019							58,090,233
Issuance of common stock, shares									6,483,776
Net loss	(11,168,449)
Unrealized gain (loss) on short-term investments	21,561
Balance at Jun. 30, 2020	18,683,907				$ 24		$ 67,093			200,079,879				23,003	(181,486,092)
Balance, shares at Jun. 30, 2020					23,770		67,093,683
Balance at Mar. 31, 2020	21,913,184				$ 51		$ 62,196			197,578,248				48,230	(175,775,541)
Balance, shares at Mar. 31, 2020					51,270		62,196,117
Stock-based compensation expense	361,477									361,477
Release of restricted stock awards	24						$ 24
Release of restricted stock awards, shares							23,751
Net share settlement of restricted stock award	(2,108)						$ (7)			(2,101)
Net share settlement of restricted stock award, shares							(6,389)
Stock issued under stock purchase plans	30,512						$ 45			30,467
Stock issued under stock purchase plans, shares							45,490
Issuance of common stock			$ 1,395,058						$ 2,085			$ 1,392,973
Issuance of common stock, shares									2,084,714
Conversion of Series A convertible preferred stock for common stock					$ (27)		$ 2,750			(2,723)
Conversion of Series A convertible preferred stock for common stock, shares					(27,500)		2,750,000
Adjustment in connection with LP Purchase Agreement commitment fee and transaction costs		$ 301,538									$ 301,538
Issuance of common stock warrants in connection with SVB Term Loan	420,000									420,000
Net loss	(5,710,551)														(5,710,551)
Unrealized gain (loss) on short-term investments	(26,136)													(26,136)
Foreign currency translation adjustment	909													909
Balance at Jun. 30, 2020	$ 18,683,907				$ 24		$ 67,093			$ 200,079,879				$ 23,003	$ (181,486,092)
Balance, shares at Jun. 30, 2020					23,770		67,093,683

Condensed Consolidated Statem_4

Condensed Consolidated Statements of Changes in Stockholders' Equity (Parenthetical) - USD ($)	3 Months Ended
	Jun. 30, 2020	Mar. 31, 2020
Statement Of Stockholders Equity [Abstract]
At the market offering, commissions and issuance costs	$ 43,000	$ 174,000
Private placement issuance costs		$ 37,075

Condensed Consolidated Statem_5

Condensed Consolidated Statements of Cash Flows - USD ($)	6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019
Operating activities
Net loss	$ (11,168,449)	$ (10,168,116)
Adjustments to reconcile net loss to net cash used in operating activities:
Depreciation and amortization	700,276	603,480
Accretion of discount on NuvoGen obligation	(6,656)	(6,483)
Provision for excess inventory	1,672	45,000
Amortization of QNAH Convertible Note issuance costs	6,505	6,727
Amortization of MidCap Credit Facility discount and issuance costs	101,482	81,096
Amortization of SVB Term Loan discount and issuance costs	3,389
Stock-based compensation expense	776,793	528,578
Employee stock purchase plan expense	10,758	33,589
Non-cash operating lease expense	306,230	194,232
Accrued interest on available-for-sale securities investments	(98,092)	(178,165)
Issuance of common stock in connection with LP Purchase Agreement	205,538
Loss on extinguishment of Midcap Credit Facility and QNAH Convertible Note	522,394
Loss on abandonment of assets	49,226
Changes in operating assets and liabilities:
Accounts receivable	2,152,714	1,031,575
Inventory	(120,575)	100,927
Prepaid expenses and other	171,774	(501,474)
Deferred offering costs	140,320
Accounts payable	(659,736)	1,172,992
Accrued liabilities	(1,047,223)	(1,784,860)
Contract liabilities	(53,284)	344,494
Operating lease liabilities	(378,840)	(270,477)
Net cash used in operating activities	(8,383,784)	(8,766,885)
Investing activities
Purchase of property and equipment	(322,372)	(319,425)
Sales, redemptions and maturities of available-for-sale securities	17,950,000	22,400,000
Purchase of available-for-sale securities	(4,272,298)	(9,290,341)
Net cash provided by investing activities	13,355,330	12,790,234
Financing activities
Proceeds from issuance of SVB Term Loan and common stock warrants	10,000,000
Payment of SVB Term Loan issuance costs	(45,934)
Proceeds from ATM Offering, net	3,830,970
Proceeds from PPP Loan	1,717,000
Proceeds from Series A convertible preferred stock in private placement	562,955
Payments on NuvoGen obligation	(421,525)	(655,855)
Proceeds from exercise of pre-funded warrants	31,768
Proceeds from shares purchased under stock purchase plans	25,133	82,345
Proceeds from exercise of stock options		120,954
Payments on financing leases	(22,845)	(25,227)
Taxes paid for net share settlement of restricted stock awards	(9,160)	(20,837)
Payment of deferred offering costs		(139,058)
Payments on Insurance Note	(80,710)
Net cash provided by (used in) financing activities	5,149,029	(637,678)
Effect of exchange rates on cash	3,805	(4,682)
Increase in cash, cash equivalents and restricted cash	10,124,380	3,380,989
Cash, cash equivalents and restricted cash at beginning of period	10,889,995	11,702,847
Cash, cash equivalents and restricted cash at end of period	21,014,375	15,083,836
Supplemental disclosure of noncash investing and financing activities
Issuance of Series A convertible preferred stock in exchange for outstanding common stock	2,424,900
Issuance of Common stock in exchange for Series A convertible preferred stock	1,622,500
Adoption of ASC 842, Leases		694,352
Operating lease right-of-use assets obtained in exchange for operating lease liabilities	21,078	894,544
Fixed asset purchases payable and accrued at period end		374,202
SVB Term Loan costs payable and accrued at period end	60,710
Supplemental cash flow information
Cash paid for interest	602,200	348,130
Cash paid for taxes	6,581	$ 6,848
QNAH Convertible Note
Financing activities
Payments for extinguishment of debt	(3,000,000)
MidCap Credit Facility
Financing activities
Payments for extinguishment of debt	$ (7,438,623)

Description of Business, Basis

Description of Business, Basis of Presentation and Principles of Consolidation	6 Months Ended
	Jun. 30, 2020
Accounting Policies [Abstract]
Description of Business, Basis of Presentation and Principles of Consolidation	Note 1. Description of Business, Basis of Presentation and Principles of Consolidation HTG Molecular Diagnostics, Inc. (the “Company”) is a provider of instruments, reagents and services for molecular profiling applications. The Company derives revenue from sales of its HTG EdgeSeq instrument system and integrated next-generation sequencing-based (“NGS-based”) HTG EdgeSeq assays, from services including sample processing and custom research use only (“RUO”) assay design and from collaborative development services. The Company operates in one segment and its customers are located primarily in the United States and Europe. For the three and six months ended June 30, 2020 approximately 40% and 36%, respectively, of the Company’s revenue was generated from sales originated by customers located outside of the United States, compared with 34% and 32% for the three and six months ended June 30, 2019, respectively. COVID-19 Pandemic On January 30, 2020, the World Health Organization (“WHO”) announced a global health emergency because of a new strain of coronavirus (“COVID-19”) originating in Wuhan, China and the risks to the international community as the virus spreads globally. In March 2020, the WHO classified the COVID-19 outbreak as a pandemic. The full impact of the COVID-19 pandemic continues to evolve as of the date of this report and likewise the full impact of the pandemic on the Company’s financial condition, liquidity and future results of operations is uncertain. Management is actively monitoring the potential impact of the global situation on its financial condition, liquidity, operations, suppliers, industry and workforce. Given the daily evolution of the COVID-19 pandemic and the global responses to curb its spread, the Company is not able to fully estimate the effects of the COVID-19 pandemic on its results of operations, financial condition or liquidity. The Company experienced a significant slowing of product and product-related services revenue generation beginning in March 2020 and believes this period of reduced revenue will continue through at least the remainder of 2020 due to disruptions to its customers’ businesses as a result of the pandemic. The extent of this impact is likely to vary from customer to customer depending upon how they are directly or indirectly impacted by local stay-at-home orders and other social distancing measures, prioritization by those customers when the immediate impacts of the pandemic have passed, and the workforce and supplier impacts that each customer has experienced during the pandemic. The Company has also experienced limited delays in its development efforts as a result of stay-at-home orders and its efforts to prioritize the safety of its employees during this pandemic. The Company believes the COVID-19 pandemic will continue to impact its productivity, supply chains, distribution networks and other areas of its operation as the pandemic continues to disrupt the Company’s business and the businesses of its vendors, partners and customers . Although the Company cannot estimate the length and gravity of the impact of the COVID-19 pandemic at this time, the COVID-19 pandemic could have a continuing adverse effect on the Company’s results of operations, financial position and liquidity for at least the remainder of 2020. On March 27, 2020, the Coronavirus Aid, Relief, and Economic Security Act (the “CARES Act”) was enacted in response to the COVID-19 pandemic. The CARES Act, among other things, permits NOL carryovers and carrybacks to offset 100% of taxable income for taxable years beginning before 2021. In addition, the CARES Act allows NOLs incurred in 2018, 2019 and 2020 to be carried back to each of the five preceding taxable years to generate a refund of previously paid income taxes. The Company does not currently expect that these provisions will have a significant impact on its condensed consolidated financial statements. While there remains uncertainty as to the ultimate impact of the COVID-19 pandemic, the Company has considered the known impacts on its business as of the date of this report and has reflected any known or expected impacts in its accompanying condensed consolidated financial statements, including consideration of potential impairment risks to its long-lived assets, potential accounts receivable collection risks and potential impacts to the Company’s overall liquidity position. Basis of Presentation The accompanying condensed consolidated financial statements have been prepared by the Company pursuant to the rules and regulations of the Securities and Exchange Commission (“SEC”) and reflect the accounts of the Company as of June 30, 2020 and for the three and six months ended June 30, 2020 and 2019. Accordingly, they do not include all of the information and footnotes required by accounting principles generally accepted in the United States of America (“U.S. GAAP”) for complete financial statements. The accompanying condensed consolidated financial statements reflect all adjustments consisting of normal recurring adjustments which, in the opinion of management, are necessary for a fair statement of the Company’s financial position and the results of its operations and cash flows, as of and for the periods presented. The accompanying condensed consolidated balance sheet at December 31, 2019 has been derived from the audited consolidated financial statements at that date but does not include all of the information and disclosures required by U.S. GAAP for annual financial statements. The results of operations for the interim periods are not necessarily indicative of the results to be expected for the full year. These condensed consolidated financial statements should be read in conjunction with the Company’s audited consolidated financial statements and notes thereto for the year ended December 31, 2019, included in the Company’s Annual Report on Form 10-K filed with the SEC on March 25, 2020. Liquidity The Company believes that its existing resources will be sufficient to fund its planned operations and expenditures for at least the next 12 months from the issuance of these condensed consolidated financial statements. However, the Company will need to raise additional capital to fund its operations and service its long-term debt obligations until its revenue reaches a level sufficient to provide for self-sustaining cash flows. There can be no assurance that additional capital will be available on acceptable terms, or at all, or that the Company’s revenue will reach a level sufficient to provide for self-sustaining cash flows. In addition, the Company must comply with a financial covenant requiring the Company to maintain a certain amount of unrestricted cash under its Loan and Security Agreement with Silicon Valley Bank (see Note 8). Principles of Consolidation The Company formed a French subsidiary, HTG Molecular Diagnostics France SARL (“HTG France”), in November 2018. The accompanying condensed consolidated financial statements include the accounts of the Company and this wholly owned subsidiary after elimination of all intercompany transactions and balances as of June 30, 2020 and December 31, 2019. Concentration Risks Financial instruments that potentially subject the Company to credit risk consist principally of cash and cash equivalents, available-for-sale debt securities and uncollateralized accounts receivable. The Company maintains the majority of its cash balances in the form of cash deposits in bank checking and money market accounts in amounts in excess of federally insured limits. Management believes, based upon the quality of the financial institution, that the credit risk with regard to these deposits is not significant. The available-for-sale debt securities are comprised primarily of investments in U.S. Treasury and high-quality corporate debt securities. The Company sells its instrument, related consumables, sample processing services, custom RUO assay design and collaborative development services primarily to biopharmaceutical companies, academic institutions and molecular labs. The Company routinely assesses the financial strength of its customers and credit losses have been minimal to date. The Company’s top three customers accounted for 17%, 12% and 10% of the Company’s total revenue for the three months ended June 30, 2020, compared with the Company’s top three customers accounting for 24%, 18% and 16% of the Company’s total revenue for the three months ended June 30, 2019. The Company’s top three customers accounted for 23%, 11% and 11% of the Company’s total revenue for the six months ended June 30, 2020 compared with the Company’s top three customers accounting for 31%, 21% and 13% for the six months ended June 30, 2019. The Company’s top two customers accounted for approximately 18% and 10% of the Company’s accounts receivable as of June 30, 2020 compared with approximately 29% and 25% of the Company’s accounts receivable as of December 31, 2019. The Company currently relies on a single supplier to supply a subcomponent used in its HTG EdgeSeq processors. A loss of this supplier could significantly delay the delivery of processors, which in turn could materially affect the Company’s ability to generate revenue.

Summary of Significant Accounti

Summary of Significant Accounting Policies	6 Months Ended
	Jun. 30, 2020
Accounting Policies [Abstract]
Summary of Significant Accounting Policies	Note 2. Summary of Significant Accounting Policies Use of Estimates The preparation of financial statements in conformity with U.S. GAAP requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities, disclosures of contingent assets and liabilities at the date of the financial statements, and the reported amounts of revenues and expenses during the reporting period. The Company’s significant estimates include revenue recognition, stock-based compensation expense, bonus accrual, income tax valuation allowances and reserves, recovery of long‑lived assets, inventory obsolescence and inventory valuation, valuation of accounts receivable and available-for-sale securities. Actual results could materially differ from those estimates, especially in light of the significant uncertainty that remains as to the full impact of the COVID-19 pandemic on the Company’s operations, as well as those of its workforce, supply chains, distribution networks and those of its customers. Significant Accounting Policies There have been no material changes in the Company’s significant accounting policies from those previously disclosed in the Company’s Annual Report on Form 10-K, filed with the SEC on March 25, 2020. Restricted Cash The following table provides a reconciliation of cash, cash equivalents and restricted cash reported within the accompanying condensed consolidated statements of cash flows. June 30, 2020 2019 Cash and cash equivalents $ 21,014,375 $ 11,813,589 Restricted cash - non-current — 3,270,247 Total cash, cash equivalents and restricted cash shown in the condensed consolidated statements of cash flows $ 21,014,375 $ 15,083,836 In October 2017, the Company received $3.0 million in gross proceeds from, and issued a subordinated convertible promissory note (the “QNAH Convertible Note”) in that principal amount to, QIAGEN North American Holdings, Inc. (“QNAH”). Amounts included in restricted cash represented those required to be set aside in escrow under the terms of the MidCap Term Loan to collateralize the payment that was due upon maturity of the QNAH Convertible Note (see Note 8). The balance of restricted cash was released to the Company on June 25, 2020 in conjunction with the extinguishment of the QNAH Convertible Note in connection with the signing of the SVB Term Loan (see Note 8). Fair Value of Financial Instruments The carrying value of financial instruments classified as current assets and current liabilities approximate fair value due to their liquidity and short-term nature. Investments that are classified as available-for-sale are recorded at fair value, which is determined using quoted market prices, broker or dealer quotations or alternative pricing sources with reasonable levels of price transparency. The carrying value of the SVB Term Loan (see Note 8) is estimated to approximate its fair value as the interest rate approximates the market rate for debt with similar terms and risk characteristics. The NuvoGen obligation is an obligation relating to an asset purchase transaction with a then-common stockholder of the Company. As of June 30, 2020, the estimated aggregate fair value of the NuvoGen obligation is approximately $4.8 million, determined using a Monte Carlo simulation with key assumptions including future revenue, volatility, discount and risk-free rates. The estimated fair value of the NuvoGen obligation represents a Level 3 measurement. Leases Effective January 1, 2019, the Company accounts for its leases under Financial Accounting Standards Board (“FASB”), Accounting Standards Codification (“ASC”) 842. Under this guidance, arrangements meeting the definition of a lease are classified as operating or financing leases and are recorded on the condensed consolidated balance sheets as both right-of-use assets and lease liabilities, calculated by discounting fixed lease payments over the lease term at the rate implicit in the lease or the Company’s incremental borrowing rate. Lease liabilities are increased by interest and reduced by payments each period, and right-of-use assets are amortized over the lease term. For operating leases, interest on the lease liability and the amortization of the right-of-use asset result in straight-line rent expense over the lease term. For financing leases, interest on the lease liability and the amortization of the right-of-use asset result in front-loaded expense over the lease term. Variable lease expenses are recorded to rent expense as incurred. In calculating the right-of-use asset and lease liability, the Company elects to combine lease and non-lease components for all classes of assets currently under lease, including facilities, copiers and computer equipment. The Company excludes short-term leases having initial terms of 12 months or less from the new guidance as an accounting policy election and recognizes rent on short-term leases on a straight-line basis over the lease term for these leases. Recently Adopted Accounting Pronouncements In August 2018, the FASB issued Accounting Standards Update (“ASU”) No. 2018-13, Fair Value Measurement – Disclosure Framework – Changes to the Disclosure Requirements for Fair Value Measurement In November 2018, the FASB issued ASU No. 2018-18, which amended ASC 808, Collaborative Arrangements Revenue from Contracts with Customers New Accounting Pronouncements The following are new FASB ASUs that had not been adopted by the Company as of June 30, 2020: In August 2020, the FASB issued ASU No. 2020-06, Debt – Debt with Conversion and other Options (Subtopic 470-20) and Derivatives and Hedging – Contracts in Entity’s Own Equity (Subtopic 815-40): Accounting for Convertible Instruments and Contracts in an Entity’s Own Equity (“ASU 2020-06”), which simplifies accounting for convertible instruments by removing major separation models required under current U.S. GAAP. ASU 2020-06 removes certain settlement conditions that are required for equity contracts to qualify for the derivative scope exception and also simplifies the diluted earnings per share calculation in certain areas. The standard is effective for public business entities, excluding entities eligible to be smaller reporting companies as defined by the SEC, for fiscal years and interim periods within those fiscal years, beginning after December 15, 2021. For all other entities, the standard will be effective for fiscal years beginning after December 15, 2023. Early adoption is permitted, but no earlier than fiscal years beginning after December 15, 2020, and adoption must be as of the beginning of the Company’s annual fiscal year. The Company is currently evaluating the impact of this standard on its consolidated financial statements and related disclosures. In March 2020, the FASB issued ASU No. 2020-04, Reference Rate Reform (Topic 848): Facilitation of the Effects of Reference Rate Reform on Financial Reporting (“ASU 2020-04”), which provides temporary optional guidance to ease the potential burden in accounting for reference rate reform. The new guidance provides optional expedients and exceptions for applying generally accepted accounting principles to transactions affected by reference rate reform if certain criteria are met. These transactions include: contract modifications, hedging relationships, and sale or transfer of debt securities classified as held-to-maturity. Entities may apply the provisions of the new standard as of the beginning of the reporting period when the election is made (i.e. as early as the first quarter 2020). Unlike other topics, the provisions of this update are only available until December 31, 2022, when the reference rate replacement activity is expected to have completed. The Company is currently evaluating the impact of this standard on its consolidated financial statements and related disclosures and has yet to elect an adoption date. In December 2019, the FASB issued ASU No. 2019-12, Income Taxes (Topic 740): Simplifying the Accounting for Income Taxes In June 2016, the FASB issued ASU No. 2016-13, Financial Instruments - Credit Losses

Inventory

Inventory	6 Months Ended
	Jun. 30, 2020
Inventory Disclosure [Abstract]
Inventory	Note 3. Inventory Inventory, net of allowance, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 Raw materials $ 1,064,150 $ 872,947 Work in process 106,098 151,351 Finished goods 247,909 284,772 Total gross inventory 1,418,157 1,309,070 Less inventory allowance (29,587 ) (39,403 ) $ 1,388,570 $ 1,269,667 For the three and six months ended June 30, 2020, the Company recorded adjustments to provision for excess inventory of $(5,275) and $1,672, respectively. For the three and six months ended June 30, 2019, the Company recorded adjustments to provision for excess inventory of $20,704 and $45,000, respectively. Adjustments in these periods to the allowance for estimated shrinkage, obsolescence and excess inventory have been included in cost of revenue in the accompanying condensed consolidated statements of operations. HTG EdgeSeq instruments at customer locations under evaluation agreements are included in finished goods inventory. Finished goods inventory under evaluation was $79,338 at both June 30, 2020 and December 31, 2019.

Fair Value Instruments

Fair Value Instruments	6 Months Ended
	Jun. 30, 2020
Fair Value Disclosures [Abstract]
Fair Value Instruments	Note 4. Fair Value Instruments Financial assets and liabilities measured at fair value are classified in their entirety in the fair value hierarchy based on the lowest level input significant to the fair value measurement. The following table classifies the Company’s financial assets and liabilities measured at fair value on a recurring basis at June 30, 2020 and December 31, 2019 in the fair value hierarchy: June 30, 2020 Level 1 Level 2 Level 3 Total Asset included in: Cash and cash equivalents Money market securities $ 20,544,558 $ — $ — $ 20,544,558 Investments available-for-sale at fair value U.S. Treasury securities 5,964,676 — — 5,964,676 Corporate debt securities — 5,887,497 — 5,887,497 Total $ 26,509,234 $ 5,887,497 $ — $ 32,396,731 December 31, 2019 Level 1 Level 2 Level 3 Total Asset included in: Cash and cash equivalents Money market securities $ 7,217,096 $ — $ — $ 7,217,096 Investments available-for-sale at fair value U.S. Treasury securities 5,961,983 — — 5,961,983 Corporate debt securities — 19,448,239 — 19,448,239 Total $ 13,179,079 $ 19,448,239 $ — $ 32,627,318 There are no other financial instruments subject to fair value measurement on a recurring basis. Transfers to and from Levels 1, 2 and 3 are recognized at the end of the reporting period. There were no transfers between levels for the three and six months ended June 30, 2020 or for the year ended December 31, 2019. Level 1 instruments include investments in money market funds and U.S. Treasuries. These instruments are valued using quoted market prices for identical unrestricted instruments in active markets. The Company defines active markets for debt instruments based on both the average daily trading volume and the number of days with trading activity. Level 2 instruments include corporate debt securities. Valuations of Level 2 instruments can be verified to quoted prices, recent trading activity for identical or similar instruments, broker or dealer quotations or alternative pricing sources with reasonable levels of price transparency. Consideration is given to the nature of the quotations (e.g., indicative or firm) and the relationship of recent market activity to the prices provided from alternative pricing sources. Fair values of these assets and liabilities are based on prices provided by independent market participants that are based on observable inputs using market-based valuation techniques. These valuation models and analytical tools use market pricing or similar instruments that are both objective and publicly available, including matrix pricing or reported trades, benchmark yields, broker/dealer quotes, issuer spreads, two-sided markets, benchmark securities, bids and/or offers. The Company did not adjust any of the valuations received from these third parties with respect to any of its Level 1 or 2 securities for the six-month period ended June 30, 2020 or the year-ended December 31, 2019 and did not have any Level 3 financial assets or liabilities during either of these periods.

Available-for-Sale Securities

Available-for-Sale Securities	6 Months Ended
	Jun. 30, 2020
Investments Debt And Equity Securities [Abstract]
Available-for-Sale Securities	Note 5. Available-for-Sale Securities The Company’s portfolio of available-for-sale securities consists of U.S. Treasuries and high credit quality corporate debt securities. The following is a summary of the Company’s available-for-sale securities at June 30, 2020 and December 31, 2019: June 30, 2020 Gross Gross Fair Value Amortized Unrealized Unrealized (Net Carrying Cost Gains Losses Amount) U.S. Treasury securities $ 5,943,124 $ 21,552 $ — $ 5,964,676 Corporate debt securities 5,887,497 — — 5,887,497 Total available-for-sale securities $ 11,830,621 $ 21,552 $ — $ 11,852,173 December 31, 2019 Gross Gross Fair Value Amortized Unrealized Unrealized (Net Carrying Cost Gains Losses Amount) U.S. Treasury securities $ 5,962,224 $ 394 $ (635 ) $ 5,961,983 Corporate debt securities 19,448,239 — - 19,448,239 Total available-for-sale securities $ 25,410,463 $ 394 $ (635 ) $ 25,410,222 There were no gross unrealized losses relating to the Company’s available-for-sale securities investments as of June 30, 2020. The net adjustment to unrealized holding gains (losses) on available-for-sale securities, net of tax in other comprehensive income totaled $(26,136) and $21,561 for the three and six months ended June 30, 2020, respectively and $819 and $4,172 for the three and six months ended June 30, 2019, respectively. Contractual maturities of debt investment securities at June 30, 2020 are shown below. Expected maturities will differ from contractual maturities because the issuers of the securities may have the right to prepay obligations without prepayment penalties. Under 1 Year 1 to 2 Years Total U.S. Treasury securities $ 5,964,676 $ — $ 5,964,676 Corporate debt securities 5,887,497 5,887,497 Total available-for-sale securities $ 11,852,173 $ — $ 11,852,173 For debt securities, the Company determines whether it intends to sell or if it is more likely than not that it will be required to sell impaired securities. This determination considers current and forecasted liquidity requirements, regulatory and capital requirements and securities portfolio management. For all impaired debt securities for which there was no intent or expected requirement to sell, the evaluation considers all available evidence to assess whether it is likely the amortized cost value will be recovered. The Company conducts a regular assessment of its debt securities with unrealized losses to determine whether securities have other-than-temporary impairment considering, among other factors, the nature of the securities, credit rating or financial condition of the issuer, the extent and duration of the unrealized loss, expected cash flows of underlying collateral, market conditions and whether the Company intends to sell or it is more likely than not that the Company will be required to sell the debt securities. As the Company did not have any unrealized losses as of June 30, 2020, there were no

Property and Equipment

Property and Equipment	6 Months Ended
	Jun. 30, 2020
Property Plant And Equipment [Abstract]
Property and Equipment	Note 6. Property and Equipment Property and equipment, net, consists of the following as of the dates indicated: June 30, December 31, 2020 2019 Furniture & fixtures $ 1,089,379 $ 1,089,371 Leasehold improvements 1,956,266 1,987,997 Equipment used in manufacturing 2,305,400 2,305,340 Equipment used in research & development 2,423,857 2,134,019 Equipment used in the field 182,762 182,762 Software 393,251 374,812 Property and equipment 8,350,915 8,074,301 Less: accumulated depreciation and amortization (6,525,724 ) (5,834,168 ) $ 1,825,191 $ 2,240,133 Depreciation and leasehold improvement amortization expense was approximately $0.4 million and $0.7 million for the three and six months ended June 30, 2020, respectively, and $0.3 million and $0.6 million for the three and six months ended June 30, 2019, respectively. In the first quarter of 2020 the Company closed its applications laboratory in Sausheim, France and, as a result, $0 and $28,130 of leasehold improvements related to the abandonment of the laboratory were written off to selling, general and administrative expense during the three and six months ended June 30, 2020, respectively.

Accrued Liabilities

Accrued Liabilities	6 Months Ended
	Jun. 30, 2020
Accrued Liabilities Current [Abstract]
Accrued Liabilities	Note 7. Accrued Liabilities Accrued liabilities consist of the following as of the dates indicated: June 30, December 31, 2020 2019 Accrued employee bonuses $ 263,114 $ 900,740 Payroll and employee benefit accruals 305,825 469,530 Accrued professional fees 158,352 43,850 Accrued interest 14,519 245,350 Accrued rebate 96,357 — Other accrued liabilities 109,215 210,826 $ 947,382 $ 1,870,296

Debt Obligations

Debt Obligations	6 Months Ended
	Jun. 30, 2020
Debt Disclosure [Abstract]
Debt Obligations	Note 8. Debt Obligations Short-term debt, net, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 Convertible note, net of debt issuance costs $ — $ 2,987,667 PPP Loan 488,526 — Insurance Note Payable 654,485 — $ 1,143,011 $ 2,987,667 Long-term debt, net, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 MidCap Term Loan payable, net of discount and debt issuance costs $ — $ 6,871,545 SVB Term Loan payable, net of discount and debt issuance costs 9,476,745 — PPP Loan 1,228,474 — $ 10,705,219 $ 6,871,545 MidCap Credit Facility On March 26, 2018 (the “MidCap Closing Date”), the Company entered into a Credit and Security Agreement (Term Loan) (the “MidCap Term Loan”) and a Credit and Security Agreement (Revolving Loan) (the “MidCap Revolving Loan” and together with the MidCap Term Loan, the “MidCap Credit Facility”) with MidCap Financial Trust, as agent. MidCap Financial Trust subsequently assigned its rights and obligations as agent to MidCap Funding IV Trust. On February 26, 2020 (the “Amendment Effective Date”), the Company entered into (i) an amendment to the MidCap Term Loan (the “MidCap Term Loan Amendment”), and (ii) an amendment to the MidCap Revolving Loan (the “MidCap Revolving Loan Amendment,” and together with the MidCap Term Loan Amendment, the “Amendments”). The MidCap Term Loan Amendment extended the interest only payments on the term loan advance by an additional 11 months, with principal on the term loan advance payable in 25 equal monthly installments beginning March 1, 2021 until paid in full on March 1, 2023. The MidCap Term Loan Amendment also provided that if the Company achieved a stated revenue milestone for the 12-month period ending on December 31, 2020, the interest-only period would be further extended by an additional four months, with principal on the term loan advance then payable in 21 equal monthly installments beginning July 1, 2021. QNAH Convertible Note Agreement In October 2017, the Company issued a subordinated convertible promissory note to QNAH in the principal amount of $3.0 million against receipt of cash proceeds equal to such principal amount. On June 25, 2020 in connection with the closing of the SVB Term Loan, the Company repaid in full the QNAH Convertible Note, in the aggregate amount of approximately $3.2 million. Extinguishment of MidCap Credit Facility and QNAH Convertible Note upon SVB Term Loan Closing On June 25, 2020, the Company repaid all principal and interest amounts outstanding under the MidCap Credit Facility in an aggregate amount equal to approximately $7.5 million, including collateral agent legal fees and prepayment fees. The repayment was funded with net proceeds from the SVB Term Loan (see description of the SVB Term Loan below). As a result of the repayment, the Company recorded a loss on extinguishment of the MidCap Credit Facility and the QNAH Convertible Note of $0.5 million, including remaining unamortized discounts of $0.3 million and prepayment and other MidCap Credit Facility and QNAH Convertible Note lender fees and issuance costs in other expense in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2020. All obligations under the MidCap Credit Facility and the QNAH Convertible Note were terminated upon extinguishment of the MidCap Credit Facility. Debt discount of approximately $0.4 million associated with the MidCap Term Loan, resulting from fees and debt issuance costs, was presented in long-term debt, net in the accompanying condensed consolidated balance sheet as of December 31, 2019. Costs incurred in connection with the issuance of the Midcap Revolving Loan of $50,970 are presented as MidCap Revolving Loan costs in other non-current assets in the accompanying condensed consolidated balance sheets as of December 31, 2019. The remaining debt discount and Midcap Revolving loan cost balances were written off upon repayment of the Midcap Credit Facility in June 2020. Amortization of the debt discount associated with the MidCap Term Loan was $55,291 and $93,718 for the three and six months ended June 30, 2020, respectively, compared with $37,940 and $73,114 for the three and six months ended June 30, 2019, respectively. Amortization of deferred MidCap Revolving Loan costs were $3,749 and $7,763 for the three and six months ended June 30, 2020, respectively, compared with $4,013 and $7,982 for the three and six months ended June 30, 2019, respectively. Amortization of both the debt discount and the revolving loan costs is included in interest expense in the accompanying condensed consolidated statements of operations. SVB Term Loan On June 24, 2020 (the “Closing Date”), the Company entered into a Loan and Security Agreement (the “Loan Agreement”), by and among the Company and Silicon Valley Bank (“SVB”), as lender, which provides a secured term loan in the principal amount of $10.0 million (the “SVB Term Loan”). The proceeds from the SVB Term Loan were fully funded on the June 25, 2020. The proceeds from the SVB Term Loan, together with cash on hand, were used to repay in full all outstanding amounts and fees due under the MidCap Credit Facility and the QNAH Convertible Note. The SVB Term Loan bears interest at a floating rate equal to the greater of 2.50% above the Prime Rate (as defined in the Loan Agreement) and 5.75%. Interest on the SVB Term Loan is due and payable monthly in arrears. The SVB Term Loan has interest-only payments through June 30, 2021. The interest only period may be extended for six months upon the achievement of an equity milestone as more fully described in the Loan Agreement. The ultimate interest-only period will be followed by equal monthly payments of principal and interest through the maturity date of December 1, 2023. Prepayments of the SVB Term Loan, in whole or in part, will be subject to early termination fees in an amount equal to 3.0% of principal prepaid if prepayment occurs on or prior to the first anniversary of the Closing Date, 2.0% of principal prepaid in prepayment occurs after the first anniversary of the Closing Date but on or prior to the second anniversary of the Closing Date, and 1.0% of principal prepaid if prepayment occurs after the second anniversary of the Closing Date and prior to the maturity date. Upon termination of the Loan Agreement, the Company is required to pay a final fee equal to 8.00% of the principal amount of the SVB Term Loan. The Company’s obligations under the Loan Agreement are secured by a security interest in substantially all of its assets, excluding intellectual property (which is subject to a negative pledge). Additionally, the Company’s future subsidiaries, if any, may be required to become co-borrowers or guarantors under the Loan Agreement. The Loan Agreement contains customary affirmative covenants and customary negative covenants limiting the Company’s ability and the ability of the Company’s subsidiaries, if any, to, among other things, dispose of assets, undergo a change in control, merge or consolidate, make acquisitions, incur debt, incur liens, pay dividends, repurchase stock and make investments, in each case subject to certain exceptions. The Company must also comply with a financial covenant requiring the Company to maintain unrestricted cash at an account with SVB of not less than the greater of (i) $12.5 million and (ii) an amount equal to six times the amount of the Company’s average monthly Cash Burn (as defined in the Loan Agreement) over the trailing three months. The Loan Agreement also contains customary events of default relating to, among other things, payment defaults, breaches of covenants, a material adverse change, delisting of the Company’s common stock, bankruptcy and insolvency, cross defaults with certain material indebtedness and certain material contracts, judgments, and inaccuracies of representations and warranties. Upon an event of default, SVB may declare all or a portion of the Company’s outstanding obligations to be immediately due and payable and exercise other rights and remedies provided for under the agreement. During the existence of an event of default, interest on the obligations could be increased by 5.0%. In connection with the Loan Agreement, the Company granted to SVB a warrant to purchase up to 643,413 shares of the Company’s common stock at a purchase price of $0.7771 per share. The warrant will expire on June 24, 2030 and may be exercised for cash or at the election of the holder on a cashless, net exercise basis. The fair value of the warrant on the date of issuance was approximately $0.4 million, determined using the Black-Scholes option-pricing model, and was recorded as a discount to the SVB Term Loan. The Company recognized approximately $1.3 million of debt discount associated with the SVB Term Loan, resulting from fees and debt issuance costs, inclusive of the fair value of warrants issued, in Long-term debt, net in the as of June 30, 2020. Amortization of the debt discount associated with the SVB Term Loan was $3,389 for both the three and six months ended June 30, 2020 and was included in interest expense in the . The remaining principal repayments due under the SVB Term Loan as of June 30, 2020 are as follows for each fiscal year: 2020 $ - 2021 2,000,000 2022 4,000,000 2023 4,000,000 Total SVB Term Loan payments 10,000,000 Less discount and deferred financing costs (1,323,255 ) Plus final fee premium 800,000 Total SVB Term Loan, net $ 9,476,745 Paycheck Protection Program Loan On April 21, 2020, the Company received proceeds from a loan in the amount of $1,717,000 (the “PPP Loan”) from SVB, as lender, pursuant to the Paycheck Protection Program (“PPP”) of the CARES Act. The PPP Loan is evidenced by a promissory note (the “Note”), which contains customary events of default relating to, among other things, payment defaults and breaches of representations, warranties or terms of the PPP Loan documents. The PPP Loan matures on April 21, 2022 and bears interest at an annual rate of approximately 1%. Beginning on March 21, 2021, the Company is required to make 14 equal monthly payments of principal and interest. The PPP Loan may be prepaid by the Company at any time prior to maturity with no prepayment penalties. The proceeds from the PPP Loan may only be used for payroll costs (including benefits), rent and utility obligations, and interest on certain of the Company’s other debt obligations. All or a portion of the PPP Loan may be forgiven by the U.S. Small Business Administration (“SBA”) upon application by the Company beginning 60 days but not later than 120 days after loan approval and upon documentation of expenditures in accordance with the SBA requirements. Under the CARES Act, loan forgiveness is available for the sum of documented payroll costs, covered rent payments, covered mortgage interest and covered utilities during the 24-week (or eight-week period at the Company’s option) beginning on the date of loan approval. For purposes of the CARES Act, payroll costs exclude compensation of an individual employee in excess of $100,000, prorated annually. Not more than 40% of the forgiven amount may be for non-payroll costs. Forgiveness is reduced if the Company’s full-time headcount declines, or if salaries and wages for employees with salaries of $100,000 or less annually are reduced by more than 25%. In the event the PPP Loan, or any portion thereof, is forgiven pursuant to the PPP, the amount forgiven is applied to outstanding principal and interest. The Company intends to apply for allowable PPP Loan forgiveness. However, there can be no assurance given that the Company will obtain forgiveness of the PPP Loan in whole or in part. In order to apply for the PPP Loan, the Company certified that, among other things, the current economic uncertainty made the PPP Loan request necessary to support its ongoing operations. In addition, PPP loans under the CARES Act may be subject to certain rules, regulations and Standard Operating Procedures (“SOPs”) applicable to the SBA’s Section 7(a) Loan Program, which includes PPP loans under the CARES Act. The interpretation and applicability of these rules, regulations and SOPs in unclear, as some of them have not been referenced in the CARES Act itself or in the guidance and interpretations issued by the SBA to date. If it is determined that the Company was not eligible to receive the PPP Loan, or that the Company has not adequately complied with the rules, regulations and SOPs applicable to the SBA’s Section 7(a) Loan Program, the Company could be subject to penalties and could be required to repay the PPP Loan in its entirety. If the Company were required to repay the PPP Loan in its entirety, the Company’s liquidity would be reduced. Insurance Note On April 27, 2020, the Company entered into a commercial financing agreement to extend the payment period related to its director and officer insurance policy (the “Insurance Note”). The Insurance Note required a down payment to be made upon signing the agreement equal to approximately $0.2 million. The remaining unpaid premium balance of $0.7 million has been financed at an annual rate of 3.61% and is being repaid beginning on June 6, 2020, in nine equal monthly payments of principal and interest. The Insurance Note contains customary events of default relating to, among other things, payment defaults and breaches of representations, warranties or terms of the Insurance Note documents. The Insurance Note may be prepaid by the Company at any time prior to maturity with no prepayment penalties.

Revenue from Contracts with Cus

Revenue from Contracts with Customers	6 Months Ended
	Jun. 30, 2020
Revenue From Contract With Customer [Abstract]
Revenue from Contracts with Customers	Note 9. Revenue from Contracts with Customers Product and Product-related Services Revenue The Company had product and product-related services revenue consisting of revenue from the sale of instruments and consumables and the use of the HTG EdgeSeq proprietary technology to process samples and design custom RUO assays for the three and six months ended June 30, 2020 and 2019 as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Product revenue: Instruments $ 257,029 $ 545,699 $ 491,654 $ 686,115 Consumables 781,304 759,470 1,327,243 1,160,288 Total product revenue 1,038,333 1,305,169 1,818,897 1,846,403 Product-related services revenue: Custom RUO assay design 343,284 1,481,318 972,466 1,849,177 RUO sample processing 346,909 1,637,881 925,300 3,391,293 Total product-related services revenue 690,193 3,119,199 1,897,766 5,240,470 Total product and product-related services revenue $ 1,728,526 $ 4,424,368 $ 3,716,663 $ 7,086,873 Because the Company’s agreements for product and product-related services revenue have an expected duration of one year or less, the Company has elected the practical expedient in ASC 606-10-50-14(a) to not disclose information about its remaining performance obligations. Sale of Instruments and Consumables The delivery of each instrument and related installation and calibration are considered to be a single performance obligation, as the HTG EdgeSeq instrument must be professionally installed and calibrated prior to use. Instrument product revenue is generally recognized upon installation and calibration of the instrument by field service engineers, which represents the point at which the customer has the ability to use the instrument and has accepted the asset. Installation generally occurs within one month of instrument shipment. The delivery of each consumable is a separate performance obligation. Consumables revenue is recognized upon transfer of control, which represents the point when the customer has legal title and the significant risks of ownership of the asset. The Company’s standard terms and conditions provide that no right of return exists for instruments and consumables, unless replacement is necessary due to delivery of defective or damaged product. Customer payment terms vary but are typically between 30 and 90 days of revenue being earned from shipment or delivery, as applicable. Shipping and handling fees charged to the Company’s customers for instruments and consumables shipped are included in the accompanying condensed consolidated statements of operations as part of product and product-related services revenue. Shipping and handling costs for sold products shipped to the Company’s customers are included in the accompanying condensed consolidated statements of operations as part of cost of product and product-related services revenue. For sales of consumables in the United States, standard delivery terms are FOB shipping point, unless otherwise specified in the customer contract, reflecting transfer of control to the customer upon shipment. Standard delivery terms for sales to customers outside of the United States are FOB delivery point, unless otherwise specified in the customer contract. The Company has elected the practical expedient to account for shipping and handling as activities to fulfill the promise to transfer the consumables. The Company uses a most likely amount approach to estimate variable consideration arising from its rebate program. The Company’s variable consideration from its rebate program is constrained. The Company provides instruments to certain customers under reagent rental agreements. Under these agreements, the Company installs an instrument in the customer’s facility without a fee and the customer agrees to purchase consumable products at a stated price over the term of the agreement; in some instances, the agreements do not contain a minimum purchase requirement. Terms range from several months to multiple years and may automatically renew in several month or multiple year increments unless either party notifies the other in advance that the agreement will not renew. The Company measures progress toward complete satisfaction of this performance obligation to provide the instrument and deliver the consumables using an output method based on the number of consumables delivered in relation to the total consumables to be provided under the reagent rental agreement. This is considered to be representative of the delivery of outputs under the arrangement and the best measure of progress because the customer benefits from the instrument only in conjunction with the consumables. The Company expects to recover the cost of the instrument under the agreement through the fees charged for consumables, to the extent sold, over the term of the agreement. In reagent rental agreements, the Company retains title to the instrument and title is transferred to the customer at no additional charge at the conclusion of the initial arrangement. The cost of the instrument is amortized on a straight-line basis over the term of the arrangement, unless there is no minimum consumable product purchase, in which case the instrument would be expensed as cost of product and product-related services RUO Sample Processing The Company also provides sample preparation and processing services and molecular profiling of retrospective cohorts for its customers through its VERI/O laboratory, whereby the customer provides samples to be processed using HTG EdgeSeq technology specified in the order. Customers are charged a per sample fee for sample processing services which is recognized as revenue upon delivery of a data file to the customer showing the results of testing and completing delivery of the agreed upon service. This is when the customer can use and benefit from the results of testing and the Company has the present right to payment. Custom RUO Assay Design and Related Agreements The Company enters into custom RUO assay design agreements that may generate up-front fees and subsequent payments that might be earned upon completion of design process phases. The Company measures progress toward complete satisfaction of its performance obligation to perform custom RUO assay design using an output method based on the costs incurred to date compared with total expected costs, as this is representative of the delivery of outputs under the arrangements and the best measure of progress. However, because in most instances the assay design fees are contingent upon completion of each phase of the design project and the decision of the customer to proceed to the next phase, the amount to be included in the transaction price and recognized as revenue is limited to that which the customer is contractually obligated to pay upon completion of that phase, which is when it is probable that a significant reversal in the amount of cumulative revenue recognized will not occur. The Company did not recognize any custom RUO assay design revenue from performance obligations that were satisfied in previous periods for either the three and six months ended June 30, 2020, compared with $0 and $68,820 for the three and six months ended June 30, 2019, respectively. Collaborative Development Services Revenue The Company enters into collaborative development services agreements with biopharmaceutical companies for the development of NGS-based companion diagnostic assays in support of and in conjunction with, biopharmaceutical companies’ drug development programs. These collaborative development services agreements may generate upfront fees, and in some cases subsequent milestone payments that may be earned upon completion of certain product development milestones or activities. The Company follows ASC 606, Revenue from Contracts with Customers and ASC 808, Collaborative Arrangements to determine the appropriate recognition of revenue under our collaborative research, development and commercialization agreements that contain multiple elements. For the three and six months ended June 30, 2020 and 2019, collaborative development services revenue was generated through statements of work entered into under the Governing Agreement with QML as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Collaborative development services $ 234,768 $ 1,371,952 $ 472,105 $ 1,912,272 Master Assay Development, Commercialization and Manufacturing Agreement In November 2016, the Company entered into the Governing Agreement, which created the framework for QML and the Company to combine their technological and commercial strengths to offer biopharmaceutical companies a complete NGS-based solution for the development, manufacture and commercialization of companion diagnostic assays. Under the Governing Agreement, the parties jointly sought companion diagnostic programs with biopharmaceutical companies, with QML entering into sponsor project agreements with interested biopharmaceutical companies for specified projects, and QML and the Company entering into statements of work which set forth the rights and obligations of QML and the Company with respect to each project. In November 2019, the Company elected to terminate the Governing Agreement with QML, effective immediately, as a result of QML’s material breach of the Governing Agreement, including QML’s failure to develop an in vitro diagnostic (“IVD”) version of its GeneReader sequencing platform for development of PDP Assays. The Company’s termination of the Governing Agreement did not terminate active statements of work under the Governing Agreement. The Company has determined that SOW Two and SOW Three (each defined below) are collaborative arrangements and that QML meets the definition of a customer under ASC 606. Additionally, each SOW is a separate contract with a single performance obligation to provide development services. Under each SOW, QML pays the Company a monthly fee for development work performed by the Company and its subcontractors (collectively, the “Monthly Fee”). The Monthly Fee is based on the employee and materials costs incurred during the month, which is subject to significant variability from period to period and unknown until the costs are incurred. Therefore, the Monthly Fee, which is based on use of hours and costs as a measure of progress, is included in the transaction price and recognized as revenue over time when the costs are incurred, and the Monthly Fee is billed to QML. It is at this time that it is probable that a significant reversal in the amount of cumulative revenue recognized will not occur. The Company and QML also share any net profits resulting from performance of the development work as determined pursuant to the Governing Agreement. Such profit-sharing payment(s) are deemed to be variable consideration using the expected value method and are included in the transaction price upon completion of the respective SOW deliverables, acceptance of corresponding deliverables, and the mutual agreement by QML and the Company on the calculation of net profit, which is when it is probable that a significant reversal in the amount of cumulative revenue recognized will not occur. Because each SOW has an expected duration of one year or less, the Company has elected the practical expedient in ASC 606-10-50-14(a) to not disclose information about its remaining performance obligations for each SOW. Statement of Work No. Two In October 2017, the Company and QML entered into the second statement of work under the Governing Agreement (“SOW Two”), which was made effective as of June 2, 2017 (“Onset Date”). The Company and QML amended SOW Two twice in August 2018 and two additional times, in September 2018 and in February 2019. SOW Two addresses development activities conducted by the Company and QML since the Onset Date and those expected to be further conducted by the parties in connection with what is expected to be a multi-stage project leading to the potential development and commercialization of an NGS-based companion diagnostic assay in support of one or more therapeutic development and commercialization programs for a third-party biopharmaceutical company. The initial-phase investigational-use-only (“IUO”) development activities under SOW Two and the first three amendments related to next phases, which include the use of the IUO assay developed in the initial-phase in a retrospective clinical trial and in additional disease indications, have been completed. The fourth amendment of SOW Two, effective as of February 5, 2019, contemplates the use of the IUO assay in multiple biopharmaceutical company clinical trials and additional development activities which could potentially be included in a future companion diagnostic regulatory submission. As of June 30, 2020, development activities agreed upon in the fourth amendment are ongoing and expected to be completed in the third quarter of 2020. Revenue of approximately $0.2 million and $0.5 million inclusive of SOW Two Monthly Fees and $0 and $50,000 of SOW Two profit-sharing payments has been included in collaborative development services revenue in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2020, respectively. Revenue of approximately $1.3 million and $1.6 million, inclusive of SOW Two Monthly Fees and approximately $0.3 million of SOW Two profit-sharing payments for both the three and six months ended June 30, 2019, has been included in collaborative development services revenue in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2019, respectively. Accounts receivable relating to SOW Two of $80,465 and $171,298 remained in the accompanying condensed consolidated balance sheets as of June 30, 2020 and December 31, 2019, respectively. Costs relating to development activities conducted by the Company pursuant to SOW Two of approximately $0.2 million and $0.4 million have been included in research and development expense in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2020, respectively, compared with approximately $0.9 million and $1.1 million for the three and six months ended June 30, 2019, respectively. Statement of Work No. Three In January 2018, the Company and QML entered into a third statement of work under the Governing Agreement (“SOW Three”) and amended SOW Three in September 2018. SOW Three relates to development activities for an NGS-based clinical-trial assay (“SOW Three Project”) in connection with a sponsor project agreement between QML and a pharmaceutical company (“Pharma Three”). Initial assay development activities under SOW Three have been completed, and the first amendment to SOW Three provides for the development of an IUO assay, subsequent retrospective testing of clinical trial samples, design verification and, subject to satisfactory achievement of relevant performance and regulatory milestones, regulatory submissions in the United States and European Union necessary for the commercialization of a companion diagnostic for a corresponding Pharma Three drug. As of June 30, 2020, the Company and QML are on hold pending customer decision as to whether to proceed with next phase contract activities. There was no revenue recognized, or development costs incurred relating to SOW Three for the three and six months ended June 30, 2020. For the three and six months ended June 30, 2019, revenue of $47,668 and $330,289 reflecting SOW Three Monthly Fees has been included in collaborative development services revenue in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2019, respectively. Accounts receivable relating to SOW Three of $0 and $760,274 remained in the condensed consolidated balance sheets as of June 30, 2020 and December 31, 2019, respectively. Costs relating to development activities conducted by the Company pursuant to SOW Three of $51,871 and $270,446 have been included in research and development expense in the condensed consolidated statements of operations for the three and six months ended June 30, 2019, respectively. Contract Liabilities The Company receives up-front payments from customers for custom RUO assay design services, and occasionally for sample processing services. Payments for instrument extended warranty contracts are made in advance as are payments for certain agreed-upon capital purchases required for collaborative development service projects. The Company recognizes such up-front payments as a contract liability. The contract liability is subsequently reduced at the point in time that the data file is delivered for sample processing services or as the Company satisfies its performance obligations over time for RUO assay design, collaborative development and extended warranty services. Contract liabilities of approximately $0.5 million and $0.6 million were included in contract liabilities – current and other non-current liabilities in the accompanying condensed consolidated balance sheets as of June 30, 2020 and December 31, 2019, respectively. The Company expects approximately $0.4 million of deferred revenue to be realized within the next 12 months and the remaining $0.1 million to be realized prior to December 31, 2021. Changes in the Company’s contract liability were as follows as of the dates indicated: Product Revenue Custom RUO Assay Design Sample Processing Total Contract Liability Balance at January 1, 2020 $ 95,148 $ 66,216 $ 438,090 $ 599,454 Deferral of revenue 273,829 337,035 67,729 678,593 Recognition of deferred revenue (272,653 ) (403,251 ) (55,971 ) (731,875 ) Balance at June 30, 2020 $ 96,324 $ — $ 449,848 $ 546,172 Product Revenue Custom RUO Assay Design Sample Processing Total Contract Liability Balance at January 1, 2019 $ 116,547 $ 50,000 $ 244,400 $ 410,947 Deferral of revenue 113,038 466,885 475,778 1,055,701 Recognition of deferred revenue (101,419 ) (424,434 ) (185,352 ) (711,205 ) Balance at June 30, 2019 $ 128,166 $ 92,451 $ 534,826 $ 755,443

Other Agreements

Other Agreements	6 Months Ended
	Jun. 30, 2020
Other Agreements [Abstract]
Other Agreements	Note 10. Other Agreements NuvoGen Obligation Pursuant to the Company’s asset purchase agreement with NuvoGen Research, LLC (“NuvoGen”), as amended, the Company is obligated to pay NuvoGen the greater of $400,000 or 6% of annual revenue until the obligation is paid in full. In addition to fixed quarterly payments of $100,000, revenue-based payments of $17,798 and $187,997 were payable as of June 30, 2020 and December 31, 2019, respectively. There have been no significant modifications to the terms and conditions of the Company’s NuvoGen obligation since the disclosures made in Part II, Item 8, Consolidated Financial Statements included in the Company’s Annual Report on Form 10-K, filed with the SEC on March 25, 2020. Remaining minimum payments to be made in 2020 include $17,798 of revenue-based payments payable as of June 30, 2020 and an estimate of the additional revenue-based payment to be made in the fourth quarter of 2020 relating to revenue generated in the third quarter of 2020, estimated using actual revenue generated in the same quarter in 2019. Minimum payments to be made in 2021 include an estimate of additional revenue-based payments to be made in the first and second quarters of 2021 relating to revenue generated in the fourth quarter of 2020 and in the first quarter of 2021, estimated using revenue generated in the same quarters in 2019 and 2020. Minimum payments for the remaining periods include only the minimum quarterly payments to be made in each year. Actual payments could vary from what is shown in the table, to the extent that 6% of the Company’s annual revenue in 2021 and beyond exceeds $400,000. The remaining minimum payments to be made to NuvoGen as of June 30, 2020 are as follows for each fiscal year: 2020 $ 442,085 2021 621,525 2022 400,000 2023 400,000 2024 400,000 2025 and beyond 2,873,564 Total NuvoGen obligation payments 5,137,174 Plus interest accretion 85,655 Total NuvoGen obligation, net $ 5,222,829 The Company has recorded the obligation at the estimated present value of the future payments using a discount rate of 2.5%, the Company’s estimate of its effective borrowing rate for similar obligations. The unamortized debt discount was $(85,655) and $(92,311) at June 30, 2020 and December 31, 2019, respectively. Discount accreted during the three and six months ended June 30, 2020 was $(3,226) and $(6,656), respectively, compared with $(3,069) and $(6,483) for the three and six months ended June 30, 2019, respectively. Other Agreements There have been no significant modifications or financial events relating to the development agreement entered into by the Company with Illumina, Inc. since the disclosures made in Part II, Item 8, Consolidated Financial Statements included in the Company’s Annual Report on Form 10-K, filed with the SEC on March 25, 2020.

Net Loss per Share

Net Loss per Share	6 Months Ended
	Jun. 30, 2020
Earnings Per Share [Abstract]
Net Loss per Share	Note 11. Net Loss Per Share Basic loss per common share is computed by dividing net loss allocable to common stockholders by the weighted average number of shares of common stock or common stock equivalents outstanding. Diluted loss per common share is computed similar to basic loss per In connection with the Securities Purchase Agreement (see Note 13), the Company issued and sold an aggregate of 5,411,687 pre-funded warrants exercisable for an aggregate of 5,411,687 shares of common stock. The total exercise price of the pre-funded warrants is $0.65 per share, $0.64 of which was pre-funded and paid to the Company upon issuance of the pre-funded warrants. The remaining exercise price of the pre-funded warrants is $0.01 per share. The remaining pre-funded warrants are immediately exercisable and do not expire. As the remaining shares underlying the pre-funded warrants are issuable for nominal consideration of $0.01 per share, The following outstanding options, warrants, restricted stock units and debt conversion option were excluded from the computation of diluted net loss per share for the periods presented because their effect would have been anti-dilutive: Six Months Ended June 30, 2020 2019 Options to purchase common stock 3,990,734 2,334,272 Common stock warrants 880,328 236,915 Restricted stock units 169,677 254,580 QNAH convertible note - 788,971

Warrants

Warrants	6 Months Ended
	Jun. 30, 2020
Proceeds From Issuance Of Preferred Stock Preference Stock And Warrants [Abstract]
Warrants	Note 12. Warrants In connection with certain of its redeemable convertible preferred stock issuances, convertible debt financings and other financing arrangements, the Company has issued warrants for shares of its common stock and various issues of its redeemable convertible preferred stock, which have since been converted to common stock warrants. In September 2019, in connection with the Securities Purchase Agreement (see Note 13), the Company issued and sold an aggregate of 5,411,687 pre-funded warrants exercisable for an aggregate of 5,411,687 shares of common stock. The total exercise price of the pre-funded warrants is $0.65 per share, $0.64 of which was pre-funded and paid to the Company upon issuance of the pre-funded warrants. The remaining exercise price of the pre-funded warrants is $0.01 per share. In March 2020, 3,176,762 of the pre-funded warrants were exercised for proceeds of $31,768. As of June 30, 2020, the remaining pre-funded warrants remain outstanding and are exercisable by the holders at any time. In connection with the SVB Term Loan Agreement (see Note 8), the Company granted to SVB a warrant to purchase up to 643,413 shares of the Company’s common stock at a purchase price of $0.7771 per share. The warrant will expire on June 24, 2030 and may be exercised for cash or at the election of the holder on a cashless, net exercise basis. The following table shows the common stock warrants outstanding as of June 30, 2020: Warrant Issuance Date Shares of Common Exercise Price/Share Expiration Date August 2014 28,713 $ 23.51 2024 December 2014 144,772 14.00 2022 March 2016 45,307 2.76 2026 March 2018 18,123 7.73 2028 September 2019 2,234,925 0.01 N/A June 2020 643,413 0.7771 2030

Stockholders Deficit

Stockholders Deficit	6 Months Ended
	Jun. 30, 2020
Equity [Abstract]
Stockholders' Deficit	Note 13. Stockholders’ Deficit Equity Offerings Securities Purchase Agreement In September 2019, concurrently with the closing of an underwritten public offering, the Company entered into a Securities Purchase Agreement (the “Securities Purchase Agreement”) with certain institutional accredited investors (the “Purchasers”), pursuant to which the Company sold to the Purchasers, in a private placement transaction, an aggregate of 5,411,687 pre-funded warrants to purchase up to an aggregate of 5,411,687 shares of our common stock (“Warrant Shares”), at a price of $0.64 per warrant (which $0.64 price relates to the pre-funded portion of the total $0.65 exercise price per share). Each pre-funded warrant has a remaining exercise price of $0.01 per share and became immediately exercisable upon issuance, subject to certain beneficial ownership limitations. The exercise price of the pre-funded warrants will be subject to adjustment in the event of any stock dividends and splits, recapitalization, reorganization or similar transaction, as described in the pre-funded warrants. The pre-funded warrants are exercisable on a “cashless” basis in certain circumstances. The pre-funded warrants and the Warrant Shares were not registered under the Securities Act and were offered pursuant to the exemption provided in Section 4(a)(2) under the Securities Act and Rule 506(b) promulgated thereunder. Pursuant to the Securities Purchase Agreement, the Company filed a registration statement on Form S-3 on October 11, 2019 to register the resale of the Warrant Shares, which registration statement was declared effective by the SEC on November 26, 2019. Each Purchaser was an “accredited investor” as defined in Rule 501(a) under the Securities Act. Cantor Fitzgerald & Co. (the “Placement Agent”) acted as the sole placement agent in connection with the private placement of the warrants. Pursuant to a Placement Agency Agreement between the Company and the Placement Agent, the Company agreed to pay the Placement Agent a cash fee equal to 6% of the gross proceeds from the sale of the pre-funded warrants, and to provide reimbursement for certain out-of-pocket expenses. Upon closing, the Company received approximately $3.1 million in net proceeds from the sale of the pre-funded warrants in the private placement, which did not include proceeds subsequently received or that may be received upon exercise of the pre-funded warrants, after deducting the Placement Agent fee and other expenses. In March 2020, 3,176,762 of the pre-funded warrants were exercised for additional proceeds of $31,768. ATM Offering In November 2019, the Company entered into a Controlled Equity Offering Sales Agreement (the “Cantor Sales Agreement”) with Cantor as the sales agent, pursuant to which the Company may offer and sell, from time to time, through Cantor, shares of its common stock, par value $0.001 per share, by any method deemed to be an “at the market offering” as defined in Rule 415(a)(4) under the Securities Act (the “ATM Offering”). The shares are offered and sold pursuant to the Company’s shelf registration statement on Form S-3 (File No. 333-229045). During the six months ended June 30, 2020, the Company sold 6,483,776 shares of common stock under the ATM Offering at then-market prices for total gross proceeds of approximately $4.0 million. After deferred offering costs included as non-current assets in the condensed balance sheets as of December 31, 2019 of approximately $0.1 million and paying sales commissions owed in connection with the ATM Offering of $0.1 million, the Company’s aggregate net proceeds for the six months ended June 30, 2020 were approximately $3.8 million. Exchange and Private Placement On February 25, 2020, the Company entered into an Exchange and Purchase Agreement (the “Exchange Agreement”) with certain accredited investors (the “Investors”) pursuant to which the Company agreed to (i) issue to the Investors an aggregate of 41,100 shares of its newly designated Series A Convertible Preferred Stock, par value $0.001 per share (“Series A Preferred”), in exchange for the Investors surrendering to the Company for cancellation an aggregate of 4,110,000 shares of its common stock (the “Exchange”) and (ii) sell and issue to the Investors an aggregate of 10,170 shares of Series A Preferred for an aggregate purchase price of $600,030, or $59.00 per share (the “Private Placement”). The common stock acquired in the Exchange was immediately retired. Each share of Series A Preferred is convertible into 100 shares of the Company’s common stock, subject to proportional adjustment and beneficial ownership limitations. In June 2020, certain of the Investors elected to convert 27,500 shares of Series A Preferred to common stock in the aggregate, resulting in the issuance of 2,750,000 shares of the Company’s common stock. The remaining 23,770 Series A Preferred shares remain outstanding as of June 30, 2020. In the event of the Company’s liquidation, dissolution or winding up, holders of Series A Preferred will participate pari passu with any distribution of proceeds to holders of the Company’s common stock. Holders of Series A Preferred are entitled to receive dividends on shares of Series A Preferred equal (on an as converted to common stock basis) to and in the same form as dividends actually paid on the Company’s common stock. Shares of Series A Preferred generally have no voting rights, except as required by law. LP Purchase Agreement On March 24, 2020, the Company entered into a purchase agreement ("LP Purchase Agreement") with Lincoln Park Capital Fund, LLC ("Lincoln Park"), pursuant to which, upon the terms and subject to the conditions and limitations set forth therein, the Company has the right to sell to Lincoln Park up to $20.0 million of shares of its common stock (“Purchase Shares”) from time to time over the 36-month term of the LP Purchase Agreement. The purchase price of the Purchase Shares will be based on recent closing prices of the Company’s common stock at the time of sale. The Company issued Lincoln Park an aggregate of 615,384 shares of its common stock as consideration for their purchase commitment pursuant to the LP Purchase Agreement. The Company did not receive cash proceeds from the issuance of such shares. The value of this commitment consideration, as well as related transaction costs of $0.1 million have been included in selling, general and administrative expense in the accompanying condensed consolidated statements of operations for the three and six months ended Stock-based Compensation A summary of the Company’s stock option activity for the six months ended June 30, 2020 is as follows: Number of Shares Weighted- Average Exercise Price Per Share Weighted- Average Remaining Contractual Life (Years) Aggregate Intrinsic Value Balance at December 31, 2019 2,904,898 $ 2.37 8.0 $ 781 Granted 1,347,500 0.69 Exercised - - $ - Forfeited (212,860 ) 1.79 Expired/Cancelled (48,804 ) 3.42 Balance at June 30, 2020 3,990,734 $ 1.82 8.1 $ 54,750 Exercisable at June 30, 2020 2,046,557 $ 2.27 7.0 $ 13,038 As of June 30, 2020, total unrecognized compensation cost related stock option awards was approximately $1.8 million, which is expected to be recognized over approximately 1.76 years. The following table summarizes restricted stock unit (“RSU”) award activity for the six months ended June 30, 2020: Number of Shares Weighted- Average Grant Date Fair Value Per Share Balance at December 31, 2019 223,745 $ 2.91 Granted 22,500 0.75 Released (59,693 ) 2.66 Forfeited (16,875 ) 3.04 Balance at June 30, 2020 169,677 $ 2.70 Vested and unissued at June 30, 2020 23,126 $ 2.73 Vested and unissued awards at June 30, 2020 represents RSU awards for which the vesting date was June 30, 2020, but for which issuance of the awards occurred in July 2020. As of June 30, 2020, the total unrecognized compensation cost related to RSU awards was approximately $0.4 million, which is expected to be recognized over approximately 1.87 years. Stock-based compensation expense recorded in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2020 and 2019 was as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Selling, general and administrative $ 338,063 $ 217,195 $ 696,075 $ 399,176 Research and development 24,913 58,234 83,418 106,684 Cost of product and product-related services revenue 3,904 11,903 8,058 22,718 $ 366,880 $ 287,332 $ 787,551 $ 528,578

Leases

Leases	6 Months Ended
	Jun. 30, 2020
Leases [Abstract]
Leases	Note 14. Leases Operating Leases T he Company leases office space and equipment under agreements classified as operating leases that expire on various dates through 2023. The Company’s most significant active leases as of June 30, 2020 are for office and manufacturing space in Tucson, Arizona, which expire in 2021. The Company also leases a development laboratory space in San Carlos, California, which expires in 2023. The Company’s leases do not include any contingent rental payments, impose any financial restrictions, or contain any residual value guarantees. Certain of the Company’s leases include renewal options and escalation clauses; renewal options have not been included in the calculation of the lease liabilities and right-of-use assets as the Company is not reasonably certain to exercise the options. Annual rent increases are included in the calculation of the operating lease right-of-use assets. Variable expenses generally represent the Company’s share of the landlord’s operating expenses and are recorded when incurred. Incremental borrowing rates used to discount future lease payments in calculating lease liabilities were estimated by reference to the rate on the Company’s existing debt, as this represents the cost of borrowing for secured loans of similar duration. The Company does not have any operating lease arrangements where it acts as a lessor. In the first quarter of 2020 the Company closed its applications laboratory in Sausheim, France and, as a result, $21,096 of operating right-of-use assets related to the abandonment of the laboratory were written off to selling, general and administrative expense during the six months ended June 30, 2020. The components of lease cost for operating leases were as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Operating leases Operating lease cost $ 182,649 $ 164,550 $ 387,876 $ 260,731 Variable lease cost 43,706 31,807 53,162 53,258 Operating lease expense 226,355 196,357 441,038 313,989 Short-term lease rent expense — — — 1,497 Total rent expense $ 226,355 $ 196,357 $ 441,038 $ 315,486 The table below summarizes other information related to the Company’s operating leases: Six Months Ended June 30, 2020 2019 Operating cash flows for operating leases $ 439,518 $ 338,089 Establishment of operating lease liabilities arising from obtaining right-of-use assets $ 21,078 $ 1,927,652 Weighted-average remaining lease term – operating leases 2.0 2.7 Weighted-average discount rate – operating leases 9.6 % 9.6 % As of June 30, 2020, remaining maturities of our operating leases, excluding short-term leases, are as follows: 2020 $ 441,781 2021 352,243 2022 281,232 2023 70,848 Total 1,146,104 Less present value discount (108,628 ) Operating lease liabilities, net $ 1,037,476 Financing Leases The Company has a number of computer and copier equipment leases that are classified as financing leases. Incremental borrowing rates used to discount future lease payments in calculating lease liabilities were estimated by reference to information received by the Company from bankers regarding estimated current borrowing rates for collateralized loans with similar amount and duration as the leases. The components of lease cost for financing leases were as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Financing leases Amortization of right-of-use assets $ 11,480 $ 12,084 $ 23,998 $ 26,780 Interest on lease liability 2,347 1,823 4,982 3,969 Total financing lease cost $ 13,827 $ 13,907 $ 28,980 $ 30,749 The table below summarizes other information related to the Company’s financing leases: Six Months Ended June 30, 2020 2019 Weighted-average remaining lease term – financing leases 3.6 2.2 Weighted-average discount rate – financing leases 9.77 % 9.77 % As of June 30, 2020, remaining maturities of our financing leases are as follows: 2020 $ 22,245 2021 28,355 2022 20,716 2023 18,396 2024 16,080 Total 105,792 Less present value discount (16,829 ) Financing lease liabilities, net $ 88,963 At June 30, 2020, the Company had financing lease liabilities net of discount of $88,963, of which $30,983 was included in other current liabilities and $57,980 was included in other non-current liabilities, and financing right-of-use assets of $85,598, which were included in property and equipment, net, in the accompanying condensed consolidated balance sheet.

Commitments and Contingencies

Commitments and Contingencies	6 Months Ended
	Jun. 30, 2020
Commitments And Contingencies Disclosure [Abstract]
Commitments and Contingencies	Note 15. Commitments and Contingencies Legal Matters The Company’s industry is characterized by frequent claims and litigation, including claims regarding intellectual property and product liability. As a result, the Company may be subject to various legal proceedings from time to time. The results of any current or future litigation cannot be predicted with certainty, and regardless of the outcome, litigation can have an adverse impact on the Company because of defense and settlement costs, diversion of management resources and other factors. Any current litigation is considered immaterial and counter claims have been assessed as remote. Product Warranty The following is a summary of the Company’s general product warranty liability, which is included in accrued liabilities in the accompanying condensed consolidated balance sheets as of the dates indicated. Expense relating to the recording of this reserve is recorded in cost of product and product-related services revenue within the accompanying condensed consolidated statements of operations. Six Months Ended June 30, 2020 2019 Beginning balance $ 94,482 $ 63,461 Cost of warranty claims (12,056 ) (36,434 ) Increase in warranty reserve 7,964 47,612 Ending balance $ 90,390 $ 74,639

Subsequent Events

Subsequent Events	6 Months Ended
	Jun. 30, 2020
Subsequent Events [Abstract]
Subsequent Events	Note 16. Subsequent Events ATM Offering From July 1 through August 7, 2020, the Company sold 3,594,196 shares of its common stock at then-market prices under the ATM Offering for gross proceeds of approximately $2.4 million. After paying sales commissions owed in connection with the ATM Offering, the Company’s aggregate net proceeds were approximately $2.3 million.

Summary of Significant Accoun_2

Summary of Significant Accounting Policies (Policies)	6 Months Ended
	Jun. 30, 2020
Accounting Policies [Abstract]
COVID-19 Pandemic	COVID-19 Pandemic On January 30, 2020, the World Health Organization (“WHO”) announced a global health emergency because of a new strain of coronavirus (“COVID-19”) originating in Wuhan, China and the risks to the international community as the virus spreads globally. In March 2020, the WHO classified the COVID-19 outbreak as a pandemic. The full impact of the COVID-19 pandemic continues to evolve as of the date of this report and likewise the full impact of the pandemic on the Company’s financial condition, liquidity and future results of operations is uncertain. Management is actively monitoring the potential impact of the global situation on its financial condition, liquidity, operations, suppliers, industry and workforce. Given the daily evolution of the COVID-19 pandemic and the global responses to curb its spread, the Company is not able to fully estimate the effects of the COVID-19 pandemic on its results of operations, financial condition or liquidity. The Company experienced a significant slowing of product and product-related services revenue generation beginning in March 2020 and believes this period of reduced revenue will continue through at least the remainder of 2020 due to disruptions to its customers’ businesses as a result of the pandemic. The extent of this impact is likely to vary from customer to customer depending upon how they are directly or indirectly impacted by local stay-at-home orders and other social distancing measures, prioritization by those customers when the immediate impacts of the pandemic have passed, and the workforce and supplier impacts that each customer has experienced during the pandemic. The Company has also experienced limited delays in its development efforts as a result of stay-at-home orders and its efforts to prioritize the safety of its employees during this pandemic. The Company believes the COVID-19 pandemic will continue to impact its productivity, supply chains, distribution networks and other areas of its operation as the pandemic continues to disrupt the Company’s business and the businesses of its vendors, partners and customers . Although the Company cannot estimate the length and gravity of the impact of the COVID-19 pandemic at this time, the COVID-19 pandemic could have a continuing adverse effect on the Company’s results of operations, financial position and liquidity for at least the remainder of 2020. On March 27, 2020, the Coronavirus Aid, Relief, and Economic Security Act (the “CARES Act”) was enacted in response to the COVID-19 pandemic. The CARES Act, among other things, permits NOL carryovers and carrybacks to offset 100% of taxable income for taxable years beginning before 2021. In addition, the CARES Act allows NOLs incurred in 2018, 2019 and 2020 to be carried back to each of the five preceding taxable years to generate a refund of previously paid income taxes. The Company does not currently expect that these provisions will have a significant impact on its condensed consolidated financial statements. While there remains uncertainty as to the ultimate impact of the COVID-19 pandemic, the Company has considered the known impacts on its business as of the date of this report and has reflected any known or expected impacts in its accompanying condensed consolidated financial statements, including consideration of potential impairment risks to its long-lived assets, potential accounts receivable collection risks and potential impacts to the Company’s overall liquidity position.
Basis of Presentation	Basis of Presentation The accompanying condensed consolidated financial statements have been prepared by the Company pursuant to the rules and regulations of the Securities and Exchange Commission (“SEC”) and reflect the accounts of the Company as of June 30, 2020 and for the three and six months ended June 30, 2020 and 2019. Accordingly, they do not include all of the information and footnotes required by accounting principles generally accepted in the United States of America (“U.S. GAAP”) for complete financial statements. The accompanying condensed consolidated financial statements reflect all adjustments consisting of normal recurring adjustments which, in the opinion of management, are necessary for a fair statement of the Company’s financial position and the results of its operations and cash flows, as of and for the periods presented. The accompanying condensed consolidated balance sheet at December 31, 2019 has been derived from the audited consolidated financial statements at that date but does not include all of the information and disclosures required by U.S. GAAP for annual financial statements. The results of operations for the interim periods are not necessarily indicative of the results to be expected for the full year. These condensed consolidated financial statements should be read in conjunction with the Company’s audited consolidated financial statements and notes thereto for the year ended December 31, 2019, included in the Company’s Annual Report on Form 10-K filed with the SEC on March 25, 2020.
Liquidity	Liquidity The Company believes that its existing resources will be sufficient to fund its planned operations and expenditures for at least the next 12 months from the issuance of these condensed consolidated financial statements. However, the Company will need to raise additional capital to fund its operations and service its long-term debt obligations until its revenue reaches a level sufficient to provide for self-sustaining cash flows. There can be no assurance that additional capital will be available on acceptable terms, or at all, or that the Company’s revenue will reach a level sufficient to provide for self-sustaining cash flows. In addition, the Company must comply with a financial covenant requiring the Company to maintain a certain amount of unrestricted cash under its Loan and Security Agreement with Silicon Valley Bank (see Note 8).
Principles of Consolidation	Principles of Consolidation The Company formed a French subsidiary, HTG Molecular Diagnostics France SARL (“HTG France”), in November 2018. The accompanying condensed consolidated financial statements include the accounts of the Company and this wholly owned subsidiary after elimination of all intercompany transactions and balances as of June 30, 2020 and December 31, 2019.
Concentration Risks	Concentration Risks Financial instruments that potentially subject the Company to credit risk consist principally of cash and cash equivalents, available-for-sale debt securities and uncollateralized accounts receivable. The Company maintains the majority of its cash balances in the form of cash deposits in bank checking and money market accounts in amounts in excess of federally insured limits. Management believes, based upon the quality of the financial institution, that the credit risk with regard to these deposits is not significant. The available-for-sale debt securities are comprised primarily of investments in U.S. Treasury and high-quality corporate debt securities. The Company sells its instrument, related consumables, sample processing services, custom RUO assay design and collaborative development services primarily to biopharmaceutical companies, academic institutions and molecular labs. The Company routinely assesses the financial strength of its customers and credit losses have been minimal to date. The Company’s top three customers accounted for 17%, 12% and 10% of the Company’s total revenue for the three months ended June 30, 2020, compared with the Company’s top three customers accounting for 24%, 18% and 16% of the Company’s total revenue for the three months ended June 30, 2019. The Company’s top three customers accounted for 23%, 11% and 11% of the Company’s total revenue for the six months ended June 30, 2020 compared with the Company’s top three customers accounting for 31%, 21% and 13% for the six months ended June 30, 2019. The Company’s top two customers accounted for approximately 18% and 10% of the Company’s accounts receivable as of June 30, 2020 compared with approximately 29% and 25% of the Company’s accounts receivable as of December 31, 2019. The Company currently relies on a single supplier to supply a subcomponent used in its HTG EdgeSeq processors. A loss of this supplier could significantly delay the delivery of processors, which in turn could materially affect the Company’s ability to generate revenue.
Use of Estimates	Use of Estimates The preparation of financial statements in conformity with U.S. GAAP requires management to make estimates and assumptions that affect the reported amounts of assets and liabilities, disclosures of contingent assets and liabilities at the date of the financial statements, and the reported amounts of revenues and expenses during the reporting period. The Company’s significant estimates include revenue recognition, stock-based compensation expense, bonus accrual, income tax valuation allowances and reserves, recovery of long‑lived assets, inventory obsolescence and inventory valuation, valuation of accounts receivable and available-for-sale securities. Actual results could materially differ from those estimates, especially in light of the significant uncertainty that remains as to the full impact of the COVID-19 pandemic on the Company’s operations, as well as those of its workforce, supply chains, distribution networks and those of its customers.
Significant Accounting Policies	Significant Accounting Policies There have been no material changes in the Company’s significant accounting policies from those previously disclosed in the Company’s Annual Report on Form 10-K, filed with the SEC on March 25, 2020.
Restricted Cash	Restricted Cash The following table provides a reconciliation of cash, cash equivalents and restricted cash reported within the accompanying condensed consolidated statements of cash flows. June 30, 2020 2019 Cash and cash equivalents $ 21,014,375 $ 11,813,589 Restricted cash - non-current — 3,270,247 Total cash, cash equivalents and restricted cash shown in the condensed consolidated statements of cash flows $ 21,014,375 $ 15,083,836 In October 2017, the Company received $3.0 million in gross proceeds from, and issued a subordinated convertible promissory note (the “QNAH Convertible Note”) in that principal amount to, QIAGEN North American Holdings, Inc. (“QNAH”). Amounts included in restricted cash represented those required to be set aside in escrow under the terms of the MidCap Term Loan to collateralize the payment that was due upon maturity of the QNAH Convertible Note (see Note 8). The balance of restricted cash was released to the Company on June 25, 2020 in conjunction with the extinguishment of the QNAH Convertible Note in connection with the signing of the SVB Term Loan (see Note 8).
Fair Value of Financial Instruments	Fair Value of Financial Instruments The carrying value of financial instruments classified as current assets and current liabilities approximate fair value due to their liquidity and short-term nature. Investments that are classified as available-for-sale are recorded at fair value, which is determined using quoted market prices, broker or dealer quotations or alternative pricing sources with reasonable levels of price transparency. The carrying value of the SVB Term Loan (see Note 8) is estimated to approximate its fair value as the interest rate approximates the market rate for debt with similar terms and risk characteristics. The NuvoGen obligation is an obligation relating to an asset purchase transaction with a then-common stockholder of the Company. As of June 30, 2020, the estimated aggregate fair value of the NuvoGen obligation is approximately $4.8 million, determined using a Monte Carlo simulation with key assumptions including future revenue, volatility, discount and risk-free rates. The estimated fair value of the NuvoGen obligation represents a Level 3 measurement.
Leases	Leases Effective January 1, 2019, the Company accounts for its leases under Financial Accounting Standards Board (“FASB”), Accounting Standards Codification (“ASC”) 842. Under this guidance, arrangements meeting the definition of a lease are classified as operating or financing leases and are recorded on the condensed consolidated balance sheets as both right-of-use assets and lease liabilities, calculated by discounting fixed lease payments over the lease term at the rate implicit in the lease or the Company’s incremental borrowing rate. Lease liabilities are increased by interest and reduced by payments each period, and right-of-use assets are amortized over the lease term. For operating leases, interest on the lease liability and the amortization of the right-of-use asset result in straight-line rent expense over the lease term. For financing leases, interest on the lease liability and the amortization of the right-of-use asset result in front-loaded expense over the lease term. Variable lease expenses are recorded to rent expense as incurred. In calculating the right-of-use asset and lease liability, the Company elects to combine lease and non-lease components for all classes of assets currently under lease, including facilities, copiers and computer equipment. The Company excludes short-term leases having initial terms of 12 months or less from the new guidance as an accounting policy election and recognizes rent on short-term leases on a straight-line basis over the lease term for these leases.
Recently Adopted and New Accounting Pronouncements	Recently Adopted Accounting Pronouncements In August 2018, the FASB issued Accounting Standards Update (“ASU”) No. 2018-13, Fair Value Measurement – Disclosure Framework – Changes to the Disclosure Requirements for Fair Value Measurement In November 2018, the FASB issued ASU No. 2018-18, which amended ASC 808, Collaborative Arrangements Revenue from Contracts with Customers New Accounting Pronouncements The following are new FASB ASUs that had not been adopted by the Company as of June 30, 2020: In August 2020, the FASB issued ASU No. 2020-06, Debt – Debt with Conversion and other Options (Subtopic 470-20) and Derivatives and Hedging – Contracts in Entity’s Own Equity (Subtopic 815-40): Accounting for Convertible Instruments and Contracts in an Entity’s Own Equity (“ASU 2020-06”), which simplifies accounting for convertible instruments by removing major separation models required under current U.S. GAAP. ASU 2020-06 removes certain settlement conditions that are required for equity contracts to qualify for the derivative scope exception and also simplifies the diluted earnings per share calculation in certain areas. The standard is effective for public business entities, excluding entities eligible to be smaller reporting companies as defined by the SEC, for fiscal years and interim periods within those fiscal years, beginning after December 15, 2021. For all other entities, the standard will be effective for fiscal years beginning after December 15, 2023. Early adoption is permitted, but no earlier than fiscal years beginning after December 15, 2020, and adoption must be as of the beginning of the Company’s annual fiscal year. The Company is currently evaluating the impact of this standard on its consolidated financial statements and related disclosures. In March 2020, the FASB issued ASU No. 2020-04, Reference Rate Reform (Topic 848): Facilitation of the Effects of Reference Rate Reform on Financial Reporting (“ASU 2020-04”), which provides temporary optional guidance to ease the potential burden in accounting for reference rate reform. The new guidance provides optional expedients and exceptions for applying generally accepted accounting principles to transactions affected by reference rate reform if certain criteria are met. These transactions include: contract modifications, hedging relationships, and sale or transfer of debt securities classified as held-to-maturity. Entities may apply the provisions of the new standard as of the beginning of the reporting period when the election is made (i.e. as early as the first quarter 2020). Unlike other topics, the provisions of this update are only available until December 31, 2022, when the reference rate replacement activity is expected to have completed. The Company is currently evaluating the impact of this standard on its consolidated financial statements and related disclosures and has yet to elect an adoption date. In December 2019, the FASB issued ASU No. 2019-12, Income Taxes (Topic 740): Simplifying the Accounting for Income Taxes In June 2016, the FASB issued ASU No. 2016-13, Financial Instruments - Credit Losses

Summary of Significant Accoun_3

Summary of Significant Accounting Policies (Tables)	6 Months Ended
	Jun. 30, 2020
Accounting Policies [Abstract]
Summary of Reconciliation of Cash, Cash Equivalents and Restricted Cash	The following table provides a reconciliation of cash, cash equivalents and restricted cash reported within the accompanying condensed consolidated statements of cash flows. June 30, 2020 2019 Cash and cash equivalents $ 21,014,375 $ 11,813,589 Restricted cash - non-current — 3,270,247 Total cash, cash equivalents and restricted cash shown in the condensed consolidated statements of cash flows $ 21,014,375 $ 15,083,836

Inventory (Tables)

Inventory (Tables)	6 Months Ended
	Jun. 30, 2020
Inventory Disclosure [Abstract]
Schedule of Inventory	Inventory, net of allowance, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 Raw materials $ 1,064,150 $ 872,947 Work in process 106,098 151,351 Finished goods 247,909 284,772 Total gross inventory 1,418,157 1,309,070 Less inventory allowance (29,587 ) (39,403 ) $ 1,388,570 $ 1,269,667

Fair Value Instruments (Tables)

Fair Value Instruments (Tables)	6 Months Ended
	Jun. 30, 2020
Fair Value Disclosures [Abstract]
Financial Assets and Liabilities Measured at Fair Value on Recurring Basis	The following table classifies the Company’s financial assets and liabilities measured at fair value on a recurring basis at June 30, 2020 and December 31, 2019 in the fair value hierarchy: June 30, 2020 Level 1 Level 2 Level 3 Total Asset included in: Cash and cash equivalents Money market securities $ 20,544,558 $ — $ — $ 20,544,558 Investments available-for-sale at fair value U.S. Treasury securities 5,964,676 — — 5,964,676 Corporate debt securities — 5,887,497 — 5,887,497 Total $ 26,509,234 $ 5,887,497 $ — $ 32,396,731 December 31, 2019 Level 1 Level 2 Level 3 Total Asset included in: Cash and cash equivalents Money market securities $ 7,217,096 $ — $ — $ 7,217,096 Investments available-for-sale at fair value U.S. Treasury securities 5,961,983 — — 5,961,983 Corporate debt securities — 19,448,239 — 19,448,239 Total $ 13,179,079 $ 19,448,239 $ — $ 32,627,318

Available-for-Sale Securities (

Available-for-Sale Securities (Tables)	6 Months Ended
	Jun. 30, 2020
Investments Debt And Equity Securities [Abstract]
Summary of Available-for-sale Securities	The Company’s portfolio of available-for-sale securities consists of U.S. Treasuries and high credit quality corporate debt securities. The following is a summary of the Company’s available-for-sale securities at June 30, 2020 and December 31, 2019: June 30, 2020 Gross Gross Fair Value Amortized Unrealized Unrealized (Net Carrying Cost Gains Losses Amount) U.S. Treasury securities $ 5,943,124 $ 21,552 $ — $ 5,964,676 Corporate debt securities 5,887,497 — — 5,887,497 Total available-for-sale securities $ 11,830,621 $ 21,552 $ — $ 11,852,173 December 31, 2019 Gross Gross Fair Value Amortized Unrealized Unrealized (Net Carrying Cost Gains Losses Amount) U.S. Treasury securities $ 5,962,224 $ 394 $ (635 ) $ 5,961,983 Corporate debt securities 19,448,239 — - 19,448,239 Total available-for-sale securities $ 25,410,463 $ 394 $ (635 ) $ 25,410,222
Summary of Contractual Maturities of Debt Investment Securities	Contractual maturities of debt investment securities at June 30, 2020 are shown below. Under 1 Year 1 to 2 Years Total U.S. Treasury securities $ 5,964,676 $ — $ 5,964,676 Corporate debt securities 5,887,497 5,887,497 Total available-for-sale securities $ 11,852,173 $ — $ 11,852,173

Property and Equipment (Tables)

Property and Equipment (Tables)	6 Months Ended
	Jun. 30, 2020
Property Plant And Equipment [Abstract]
Summary of Property and Equipment	Property and equipment, net, consists of the following as of the dates indicated: June 30, December 31, 2020 2019 Furniture & fixtures $ 1,089,379 $ 1,089,371 Leasehold improvements 1,956,266 1,987,997 Equipment used in manufacturing 2,305,400 2,305,340 Equipment used in research & development 2,423,857 2,134,019 Equipment used in the field 182,762 182,762 Software 393,251 374,812 Property and equipment 8,350,915 8,074,301 Less: accumulated depreciation and amortization (6,525,724 ) (5,834,168 ) $ 1,825,191 $ 2,240,133

Accrued Liabilities (Tables)

Accrued Liabilities (Tables)	6 Months Ended
	Jun. 30, 2020
Accrued Liabilities Current [Abstract]
Summary of Accrued Liabilities	Accrued liabilities consist of the following as of the dates indicated: June 30, December 31, 2020 2019 Accrued employee bonuses $ 263,114 $ 900,740 Payroll and employee benefit accruals 305,825 469,530 Accrued professional fees 158,352 43,850 Accrued interest 14,519 245,350 Accrued rebate 96,357 — Other accrued liabilities 109,215 210,826 $ 947,382 $ 1,870,296

Debt Obligations (Tables)

Debt Obligations (Tables)	6 Months Ended
	Jun. 30, 2020
Debt Disclosure [Abstract]
Schedule of Short Term Debt, Net	Short-term debt, net, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 Convertible note, net of debt issuance costs $ — $ 2,987,667 PPP Loan 488,526 — Insurance Note Payable 654,485 — $ 1,143,011 $ 2,987,667
Schedule of Long Term Debt, Net	Long-term debt, net, consisted of the following as of the dates indicated: June 30, December 31, 2020 2019 MidCap Term Loan payable, net of discount and debt issuance costs $ — $ 6,871,545 SVB Term Loan payable, net of discount and debt issuance costs 9,476,745 — PPP Loan 1,228,474 — $ 10,705,219 $ 6,871,545
Schedule of Remaining Principal Repayments due Under SVB Term Loan	The remaining principal repayments due under the SVB Term Loan as of June 30, 2020 are as follows for each fiscal year: 2020 $ - 2021 2,000,000 2022 4,000,000 2023 4,000,000 Total SVB Term Loan payments 10,000,000 Less discount and deferred financing costs (1,323,255 ) Plus final fee premium 800,000 Total SVB Term Loan, net $ 9,476,745

Revenue from Contracts with C_2

Revenue from Contracts with Customers (Tables)	6 Months Ended
	Jun. 30, 2020
Revenue From Contract With Customer [Abstract]
Product and Product-Related Service Revenue from Sale of Instruments and Consumables	Product and Product-related Services Revenue The Company had product and product-related services revenue consisting of revenue from the sale of instruments and consumables and the use of the HTG EdgeSeq proprietary technology to process samples and design custom RUO assays for the three and six months ended June 30, 2020 and 2019 as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Product revenue: Instruments $ 257,029 $ 545,699 $ 491,654 $ 686,115 Consumables 781,304 759,470 1,327,243 1,160,288 Total product revenue 1,038,333 1,305,169 1,818,897 1,846,403 Product-related services revenue: Custom RUO assay design 343,284 1,481,318 972,466 1,849,177 RUO sample processing 346,909 1,637,881 925,300 3,391,293 Total product-related services revenue 690,193 3,119,199 1,897,766 5,240,470 Total product and product-related services revenue $ 1,728,526 $ 4,424,368 $ 3,716,663 $ 7,086,873
Schedule of Collaborative Development Services	Collaborative Development Services Revenue The Company enters into collaborative development services agreements with biopharmaceutical companies for the development of NGS-based companion diagnostic assays in support of and in conjunction with, biopharmaceutical companies’ drug development programs. These collaborative development services agreements may generate upfront fees, and in some cases subsequent milestone payments that may be earned upon completion of certain product development milestones or activities. The Company follows ASC 606, Revenue from Contracts with Customers and ASC 808, Collaborative Arrangements to determine the appropriate recognition of revenue under our collaborative research, development and commercialization agreements that contain multiple elements. For the three and six months ended June 30, 2020 and 2019, collaborative development services revenue was generated through statements of work entered into under the Governing Agreement with QML as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Collaborative development services $ 234,768 $ 1,371,952 $ 472,105 $ 1,912,272
Schedule of Changes in Contract Liability	Changes in the Company’s contract liability were as follows as of the dates indicated: Product Revenue Custom RUO Assay Design Sample Processing Total Contract Liability Balance at January 1, 2020 $ 95,148 $ 66,216 $ 438,090 $ 599,454 Deferral of revenue 273,829 337,035 67,729 678,593 Recognition of deferred revenue (272,653 ) (403,251 ) (55,971 ) (731,875 ) Balance at June 30, 2020 $ 96,324 $ — $ 449,848 $ 546,172 Product Revenue Custom RUO Assay Design Sample Processing Total Contract Liability Balance at January 1, 2019 $ 116,547 $ 50,000 $ 244,400 $ 410,947 Deferral of revenue 113,038 466,885 475,778 1,055,701 Recognition of deferred revenue (101,419 ) (424,434 ) (185,352 ) (711,205 ) Balance at June 30, 2019 $ 128,166 $ 92,451 $ 534,826 $ 755,443

Other Agreements (Tables)

Other Agreements (Tables)	6 Months Ended
	Jun. 30, 2020
Other Agreements [Abstract]
Schedule of Remaining Minimum Principal Payments Due	Remaining minimum payments to be made in 2020 include $17,798 of revenue-based payments payable as of June 30, 2020 and an estimate of the additional revenue-based payment to be made in the fourth quarter of 2020 relating to revenue generated in the third quarter of 2020, estimated using actual revenue generated in the same quarter in 2019. Minimum payments to be made in 2021 include an estimate of additional revenue-based payments to be made in the first and second quarters of 2021 relating to revenue generated in the fourth quarter of 2020 and in the first quarter of 2021, estimated using revenue generated in the same quarters in 2019 and 2020. Minimum payments for the remaining periods include only the minimum quarterly payments to be made in each year. Actual payments could vary from what is shown in the table, to the extent that 6% of the Company’s annual revenue in 2021 and beyond exceeds $400,000. The remaining minimum payments to be made to NuvoGen as of June 30, 2020 are as follows for each fiscal year: 2020 $ 442,085 2021 621,525 2022 400,000 2023 400,000 2024 400,000 2025 and beyond 2,873,564 Total NuvoGen obligation payments 5,137,174 Plus interest accretion 85,655 Total NuvoGen obligation, net $ 5,222,829

Net Loss Per Share (Tables)

Net Loss Per Share (Tables)	6 Months Ended
	Jun. 30, 2020
Earnings Per Share [Abstract]
Outstanding Options, Warrants, Restricted Stock Units and Debt Conversion Option Excluded from Computation of Diluted Net Loss per Share	The following outstanding options, warrants, restricted stock units and debt conversion option were excluded from the computation of diluted net loss per share for the periods presented because their effect would have been anti-dilutive: Six Months Ended June 30, 2020 2019 Options to purchase common stock 3,990,734 2,334,272 Common stock warrants 880,328 236,915 Restricted stock units 169,677 254,580 QNAH convertible note - 788,971

Warrants (Tables)

Warrants (Tables)	6 Months Ended
	Jun. 30, 2020
Proceeds From Issuance Of Preferred Stock Preference Stock And Warrants [Abstract]
Summary of Outstanding Warrants	The following table shows the common stock warrants outstanding as of June 30, 2020: Warrant Issuance Date Shares of Common Exercise Price/Share Expiration Date August 2014 28,713 $ 23.51 2024 December 2014 144,772 14.00 2022 March 2016 45,307 2.76 2026 March 2018 18,123 7.73 2028 September 2019 2,234,925 0.01 N/A June 2020 643,413 0.7771 2030

Stockholders Deficit (Tables)

Stockholders Deficit (Tables)	6 Months Ended
	Jun. 30, 2020
Equity [Abstract]
Summary of Stock Option Plans Activity	A summary of the Company’s stock option activity for the six months ended June 30, 2020 is as follows: Number of Shares Weighted- Average Exercise Price Per Share Weighted- Average Remaining Contractual Life (Years) Aggregate Intrinsic Value Balance at December 31, 2019 2,904,898 $ 2.37 8.0 $ 781 Granted 1,347,500 0.69 Exercised - - $ - Forfeited (212,860 ) 1.79 Expired/Cancelled (48,804 ) 3.42 Balance at June 30, 2020 3,990,734 $ 1.82 8.1 $ 54,750 Exercisable at June 30, 2020 2,046,557 $ 2.27 7.0 $ 13,038
Summary of Restricted Stock Unit ("RSU") Award Activity	The following table summarizes restricted stock unit (“RSU”) award activity for the six months ended June 30, 2020: Number of Shares Weighted- Average Grant Date Fair Value Per Share Balance at December 31, 2019 223,745 $ 2.91 Granted 22,500 0.75 Released (59,693 ) 2.66 Forfeited (16,875 ) 3.04 Balance at June 30, 2020 169,677 $ 2.70 Vested and unissued at June 30, 2020 23,126 $ 2.73
Summary of Stock-Based Compensation Recorded in the Condensed Consolidated Statements of Operations	Stock-based compensation expense recorded in the accompanying condensed consolidated statements of operations for the three and six months ended June 30, 2020 and 2019 was as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Selling, general and administrative $ 338,063 $ 217,195 $ 696,075 $ 399,176 Research and development 24,913 58,234 83,418 106,684 Cost of product and product-related services revenue 3,904 11,903 8,058 22,718 $ 366,880 $ 287,332 $ 787,551 $ 528,578

Leases (Tables)

Leases (Tables)	6 Months Ended
	Jun. 30, 2020
Leases [Abstract]
Summary of Components of Lease Cost	The components of lease cost for operating leases were as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Operating leases Operating lease cost $ 182,649 $ 164,550 $ 387,876 $ 260,731 Variable lease cost 43,706 31,807 53,162 53,258 Operating lease expense 226,355 196,357 441,038 313,989 Short-term lease rent expense — — — 1,497 Total rent expense $ 226,355 $ 196,357 $ 441,038 $ 315,486 The components of lease cost for financing leases were as follows: Three Months Ended June 30, Six Months Ended June 30, 2020 2019 2020 2019 Financing leases Amortization of right-of-use assets $ 11,480 $ 12,084 $ 23,998 $ 26,780 Interest on lease liability 2,347 1,823 4,982 3,969 Total financing lease cost $ 13,827 $ 13,907 $ 28,980 $ 30,749
Summary of Other Information Related to Operating Leases	The table below summarizes other information related to the Company’s operating leases: Six Months Ended June 30, 2020 2019 Operating cash flows for operating leases $ 439,518 $ 338,089 Establishment of operating lease liabilities arising from obtaining right-of-use assets $ 21,078 $ 1,927,652 Weighted-average remaining lease term – operating leases 2.0 2.7 Weighted-average discount rate – operating leases 9.6 % 9.6 %
Summary of Remaining Maturities of Operating Leases, Excluding Short-term Leases	As of June 30, 2020, remaining maturities of our operating leases, excluding short-term leases, are as follows: 2020 $ 441,781 2021 352,243 2022 281,232 2023 70,848 Total 1,146,104 Less present value discount (108,628 ) Operating lease liabilities, net $ 1,037,476
Summary of Other Information Related to Financing Leases	The table below summarizes other information related to the Company’s financing leases: Six Months Ended June 30, 2020 2019 Weighted-average remaining lease term – financing leases 3.6 2.2 Weighted-average discount rate – financing leases 9.77 % 9.77 %
Summary of Remaining Maturities of Financing Leases	As of June 30, 2020, remaining maturities of our financing leases are as follows: 2020 $ 22,245 2021 28,355 2022 20,716 2023 18,396 2024 16,080 Total 105,792 Less present value discount (16,829 ) Financing lease liabilities, net $ 88,963

Commitments and Contingencies (

Commitments and Contingencies (Tables)	6 Months Ended
	Jun. 30, 2020
Commitments And Contingencies Disclosure [Abstract]
Summary of Product Warranty Liability	The following is a summary of the Company’s general product warranty liability, which is included in accrued liabilities in the accompanying condensed consolidated balance sheets as of the dates indicated. Expense relating to the recording of this reserve is recorded in cost of product and product-related services revenue within the accompanying condensed consolidated statements of operations. Six Months Ended June 30, 2020 2019 Beginning balance $ 94,482 $ 63,461 Cost of warranty claims (12,056 ) (36,434 ) Increase in warranty reserve 7,964 47,612 Ending balance $ 90,390 $ 74,639

Description of Business, Basi_2

Description of Business, Basis of Presentation and Principles of Consolidation - Additional Information (Details)	Mar. 27, 2020	Jun. 30, 2020Customer	Jun. 30, 2019Customer	Jun. 30, 2020SegmentCustomer	Jun. 30, 2019Customer	Dec. 31, 2019Customer
Significant Accounting Policies [Line Items]
Number of operating segments | Segment				1
CARES Act of 2020 percentage of NOL carryovers and carrybacks offsetting taxable income	100.00%
CARES act of 2020, net operating loss carryback period	5 years
Sales Revenue, Net | Customer Concentration Risk
Significant Accounting Policies [Line Items]
Number of customers		3	3	3	3
Sales Revenue, Net | Customer Concentration Risk | Customers Located Outside Of United States
Significant Accounting Policies [Line Items]
Sales revenue percentage		40.00%	34.00%	36.00%	32.00%
Sales Revenue, Net | Customer Concentration Risk | Customer One
Significant Accounting Policies [Line Items]
Sales revenue percentage		17.00%	24.00%	23.00%	31.00%
Sales Revenue, Net | Customer Concentration Risk | Customer Two
Significant Accounting Policies [Line Items]
Sales revenue percentage		12.00%	18.00%	11.00%	21.00%
Sales Revenue, Net | Customer Concentration Risk | Customer Three
Significant Accounting Policies [Line Items]
Sales revenue percentage		10.00%	16.00%	11.00%	13.00%
Accounts Receivable | Customer Concentration Risk
Significant Accounting Policies [Line Items]
Number of customers				2		2
Accounts Receivable | Customer Concentration Risk | Customer One
Significant Accounting Policies [Line Items]
Sales revenue percentage				18.00%		29.00%
Accounts Receivable | Customer Concentration Risk | Customer Two
Significant Accounting Policies [Line Items]
Sales revenue percentage				10.00%		25.00%

Summary of Significant Accoun_4

Summary of Significant Accounting Policies - Summary of Reconciliation of Cash, Cash Equivalents and Restricted Cash (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019	Jun. 30, 2019
Accounting Policies [Abstract]
Cash and cash equivalents	$ 21,014,375	$ 7,619,748	$ 11,813,589
Restricted cash - non-current			3,270,247
Total cash, cash equivalents and restricted cash shown in the condensed consolidated statements of cash flows	$ 21,014,375		$ 15,083,836

Summary of Significant Accoun_5

Summary of Significant Accounting Policies - Additional Information (Details) - USD ($) $ in Millions	1 Months Ended
	Oct. 31, 2017	Jun. 30, 2020
ASU 2018-13
Significant Accounting Policies [Line Items]
Change in accounting principle, accounting standards update, adopted		true
Change in accounting principle, accounting standards update, adoption date		Jan. 1, 2020
ASU 2018-18
Significant Accounting Policies [Line Items]
Change in accounting principle, accounting standards update, adopted		true
Change in accounting principle, accounting standards update, adoption date		Jan. 1, 2020
NuvoGen Asset Purchase Agreement
Significant Accounting Policies [Line Items]
Convertible debt, fair value		$ 4.8
QIAGEN North American Holdings, Inc. | Convertible Promissory Note
Significant Accounting Policies [Line Items]
Gross proceeds from issuance of subordinated notes	$ 3

Inventory - Schedule of Invento

Inventory - Schedule of Inventory (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Inventory Disclosure [Abstract]
Raw materials	$ 1,064,150	$ 872,947
Work in process	106,098	151,351
Finished goods	247,909	284,772
Total gross inventory	1,418,157	1,309,070
Less inventory allowance	(29,587)	(39,403)
Inventory, net	$ 1,388,570	$ 1,269,667

Inventory - Additional Informat

Inventory - Additional Information (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019	Dec. 31, 2019
Inventory [Line Items]
Provision for excess inventory	$ (5,275)	$ 20,704	$ 1,672	$ 45,000
Finished goods inventory	247,909		247,909		$ 284,772
HTG EdgeSeq
Inventory [Line Items]
Finished goods inventory	$ 79,338		$ 79,338		$ 79,338

Fair Value Instruments - Financ

Fair Value Instruments - Financial Assets and Liabilities Measured at Fair Value on Recurring Basis (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Asset included in:
Investments available-for-sale at fair value	$ 11,852,173	$ 25,410,222
U.S. Treasury Securities
Asset included in:
Investments available-for-sale at fair value	5,964,676	5,961,983
Corporate Debt Securities
Asset included in:
Investments available-for-sale at fair value	5,887,497	19,448,239
Fair Value, Measurements, Recurring
Asset included in:
Financial Assets	32,396,731	32,627,318
Fair Value, Measurements, Recurring | Money Market Securities
Asset included in:
Cash and cash equivalents	20,544,558	7,217,096
Fair Value, Measurements, Recurring | U.S. Treasury Securities
Asset included in:
Investments available-for-sale at fair value	5,964,676	5,961,983
Fair Value, Measurements, Recurring | Corporate Debt Securities
Asset included in:
Investments available-for-sale at fair value	5,887,497	19,448,239
Fair Value, Measurements, Recurring | Level 1
Asset included in:
Financial Assets	26,509,234	13,179,079
Fair Value, Measurements, Recurring | Level 1 | Money Market Securities
Asset included in:
Cash and cash equivalents	20,544,558	7,217,096
Fair Value, Measurements, Recurring | Level 1 | U.S. Treasury Securities
Asset included in:
Investments available-for-sale at fair value	5,964,676	5,961,983
Fair Value, Measurements, Recurring | Level 2
Asset included in:
Financial Assets	5,887,497	19,448,239
Fair Value, Measurements, Recurring | Level 2 | Corporate Debt Securities
Asset included in:
Investments available-for-sale at fair value	$ 5,887,497	$ 19,448,239

Fair Value Instruments - Additi

Fair Value Instruments - Additional Information (Details) - USD ($)	3 Months Ended	6 Months Ended	12 Months Ended
	Jun. 30, 2020	Jun. 30, 2020	Dec. 31, 2019
Fair Value Disclosures [Abstract]
Fair value assets, Level 1 to Level 2 transfers, Amount	$ 0	$ 0	$ 0
Fair value, assets, Level 2 to Level 1 transfers, Amount	0	0	0
Fair value, liabilities, Level 1 to Level 2 transfers, Amount	0	0	0
Fair value, liabilities, Level 2 to Level 1 transfers, Amount	0	0	0
Fair value, measurement with unobservable inputs reconciliation, recurring basis, asset transfers into Level 3	0	0	0
Fair value, measurement with unobservable inputs reconciliation, recurring Basis, asset, transfers out of Level 3	0	0	0
Fair Value, measurement with unobservable inputs reconciliation, liability, transfers into Level 3	0	0	0
Fair Value, measurement with unobservable inputs reconciliation, liability, transfers out of Level 3	$ 0	$ 0	$ 0

Available-for-Sale Securities -

Available-for-Sale Securities - Summary of Available-for-sale Securities (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Schedule Of Available For Sale Securities [Line Items]
Amortized Cost	$ 11,830,621	$ 25,410,463
Gross Unrealized Gains	21,552	394
Gross Unrealized Losses	0	(635)
Fair Value (Net Carrying Amount)	11,852,173	25,410,222
Corporate Debt Securities
Schedule Of Available For Sale Securities [Line Items]
Amortized Cost	5,887,497	19,448,239
Fair Value (Net Carrying Amount)	5,887,497	19,448,239
U.S. Treasury Securities
Schedule Of Available For Sale Securities [Line Items]
Amortized Cost	5,943,124	5,962,224
Gross Unrealized Gains	21,552	394
Gross Unrealized Losses		(635)
Fair Value (Net Carrying Amount)	$ 5,964,676	$ 5,961,983

Available-for-Sale Securities_2

Available-for-Sale Securities - Additional Information (Details) - USD ($)	3 Months Ended				6 Months Ended
	Jun. 30, 2020	Mar. 31, 2020	Jun. 30, 2019	Mar. 31, 2019	Jun. 30, 2020	Jun. 30, 2019	Dec. 31, 2019
Investments Debt And Equity Securities [Abstract]
Available-for-sale securities investments, gross unrealized losses	$ 0				$ 0		$ 635
Unrealized gain (losses) on short and long-term investments	$ (26,136)	$ 47,697	$ 819	$ 3,353	21,561	$ 4,172
Other-than-temporary impairment					$ 0

Available-for-Sale Securities_3

Available-for-Sale Securities - Summary of Contractual Maturities of Debt Investment Securities (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Schedule Of Available For Sale Securities [Line Items]
Under 1 Year	$ 11,852,173
Fair Value (Net Carrying Amount)	11,852,173	$ 25,410,222
U.S. Treasury Securities
Schedule Of Available For Sale Securities [Line Items]
Under 1 Year	5,964,676
Fair Value (Net Carrying Amount)	5,964,676	5,961,983
Corporate Debt Securities
Schedule Of Available For Sale Securities [Line Items]
Under 1 Year	5,887,497
Fair Value (Net Carrying Amount)	$ 5,887,497	$ 19,448,239

Property and Equipment - Summar

Property and Equipment - Summary of Property and Equipment (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Property Plant And Equipment [Abstract]
Furniture & fixtures	$ 1,089,379	$ 1,089,371
Leasehold improvements	1,956,266	1,987,997
Equipment used in manufacturing	2,305,400	2,305,340
Equipment used in research & development	2,423,857	2,134,019
Equipment used in the field	182,762	182,762
Software	393,251	374,812
Property and equipment	8,350,915	8,074,301
Less: accumulated depreciation and amortization	(6,525,724)	(5,834,168)
Property and equipment, net	$ 1,825,191	$ 2,240,133

Property and Equipment - Additi

Property and Equipment - Additional Information (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Property Plant And Equipment [Line Items]
Depreciation and leasehold improvement amortization expense	$ 400,000	$ 300,000	$ 700,276	$ 603,480
Sausheim, France | Selling, General and Administrative Expense
Property Plant And Equipment [Line Items]
Leasehold improvements written off related to abandonment of laboratory	$ 0		$ 28,130

Accrued Liabilities - Summary o

Accrued Liabilities - Summary of Accrued Liabilities (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Payables And Accruals [Abstract]
Accrued employee bonuses	$ 263,114	$ 900,740
Payroll and employee benefit accruals	305,825	469,530
Accrued professional fees	158,352	43,850
Accrued interest	14,519	245,350
Accrued rebate	96,357
Other accrued liabilities	109,215	210,826
Total accrued liabilities	$ 947,382	$ 1,870,296

Debt Obligations - Schedule of

Debt Obligations - Schedule of Short Term Debt, Net (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Debt Instrument [Line Items]
Convertible note, net of debt issuance costs		$ 2,987,667
Insurance Note Payable	$ 654,485
Short term debt	1,143,011	$ 2,987,667
PPP Loan
Debt Instrument [Line Items]
PPP Loan	$ 488,526

Debt Obligations - Schedule o_2

Debt Obligations - Schedule of Long Term Debt, Net (Details) - USD ($)	Jun. 30, 2020	Dec. 31, 2019
Debt Instrument [Line Items]
Long term debt	$ 10,705,219	$ 6,871,545
SVB Term Loan
Debt Instrument [Line Items]
Loan payable, net of discount and debt issuance costs	9,476,745
PPP Loan
Debt Instrument [Line Items]
PPP Loan	$ 1,228,474
MidCap Credit Facility
Debt Instrument [Line Items]
Loan payable, net of discount and debt issuance costs		$ 6,871,545

Debt Obligations - Additional I

Debt Obligations - Additional Information (Details) - USD ($)	Jun. 25, 2020	Jun. 24, 2020	Apr. 27, 2020	Apr. 21, 2020	Feb. 26, 2020	Oct. 31, 2017	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019	Dec. 31, 2019
Debt Instrument [Line Items]
Loss on extinguishment of MidCap Credit Facility and QNAH Convertible Note							$ (522,394)		$ (522,394)
Proceeds from Paycheck Protection Program Loan									1,717,000
Insurance Note
Debt Instrument [Line Items]
Debt instrument, interest rate			3.61%
Loan repayment start date			Jun. 6, 2020
Loan term			9 months
Loan prepayment penalties			$ 0
Down payment on insurance note			200,000
Premium payable on insurance note			$ 700,000
SVB Term Loan
Debt Instrument [Line Items]
Debt discount							1,323,255		1,323,255
Debt instrument, amortization expense							$ 3,389		$ 3,389
Debt instrument, principal amount		$ 10,000,000
Debt instrument, interest rate		5.75%
Extend the interest-only payments		6 months
Loan maturity date		Dec. 1, 2023
Debt instrument early termination fee percentage		3.00%
Debt instrument second anniversary early termination fee percentage		2.00%
Debt instrument after second anniversary early termination fee percentage		1.00%
Debt instrument final payment percentage		8.00%
Debt instrument, covenants unrestricted cash		$ 12,500,000
Debt instrument increase in interest on obligation percentage		5.00%
Warrant to purchase common stock, shares		643,413
Warrant purchase price		$ 0.7771					$ 0.7771		$ 0.7771
Warrant expiration date		Jun. 24, 2030
Fair value of warrants		$ 400,000
SVB Term Loan | Prime Rate | Minimum
Debt Instrument [Line Items]
Debt instrument, interest at floating rate		2.50%
Paycheck Protection Program Loan
Debt Instrument [Line Items]
Debt instrument, interest rate				1.00%
Loan maturity date				Apr. 21, 2022
Loan repayment start date				Mar. 21, 2021
Loan term				14 months
Loan prepayment penalties				$ 0
Payroll compensation cost maximum exemption limit				$ 100,000
Paycheck Protection Program Loan | Small Business Administration
Debt Instrument [Line Items]
Application for loan forgiveness start period				60 days
Application for loan forgiveness end period				120 days
Paycheck Protection Program Loan | Silicon Valley Bank
Debt Instrument [Line Items]
Proceeds from Paycheck Protection Program Loan				$ 1,717,000
Paycheck Protection Program Loan | Minimum
Debt Instrument [Line Items]
Annual salary reduction rate				25.00%
Paycheck Protection Program Loan | Maximum
Debt Instrument [Line Items]
Percentage of non-payroll costs forgiven				40.00%
Salaries and wages				$ 100,000
Convertible Promissory Note | QIAGEN North American Holdings, Inc.
Debt Instrument [Line Items]
Cash proceeds from issuance of subordinated notes						$ 3,000,000
Repayments of aggregate amount	$ 3,200,000
MidCap Credit Facility
Debt Instrument [Line Items]
Debt instrument interest only payment period					11 months
Debt Instrument, Frequency of Periodic Payment									25 equal monthly installments
Debt instrument interest only payment period if revenue milestone achieved					4 months
Debt instrument frequency of periodic payment if revenue milestone achieved									21 equal monthly installments
Repayment of outstanding principal and interest	$ 7,500,000								$ 7,438,623
Debt discount											$ 400,000
Debt instrument, amortization expense							$ 55,291	$ 37,940	93,718	$ 73,114
MidCap Credit Facility | QIAGEN North American Holdings, Inc.
Debt Instrument [Line Items]
Loss on extinguishment of MidCap Credit Facility and QNAH Convertible Note							500,000		500,000
Unamortized discounts							300,000		300,000
Revolving Credit Facility
Debt Instrument [Line Items]
Revolving loan cost											$ 50,970
Amortization of deferred financing costs							$ 3,749	$ 4,013	$ 7,763	$ 7,982

Debt Obligations - Schedule o_3

Debt Obligations - Schedule of Remaining Principal Repayments due Under SVB Term Loan (Details) - SVB Term Loan	Jun. 30, 2020USD ($)
Debt Instrument [Line Items]
2021	$ 2,000,000
2022	4,000,000
2023	4,000,000
Total SVB Term Loan payments	10,000,000
Less discount and deferred financing costs	(1,323,255)
Plus final fee premium	800,000
Total SVB Term Loan, net	$ 9,476,745

Revenue from Contracts with C_3

Revenue from Contracts with Customers - Product and Product-Related Service Revenue from Sale of Instruments and Consumables (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	$ 1,963,294	$ 5,796,320	$ 4,188,768	$ 8,999,145
Product and Product-related Services
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	1,728,526	4,424,368	3,716,663	7,086,873
HTG EdgeSeq | Instruments
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	257,029	545,699	491,654	686,115
HTG EdgeSeq | Consumables
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	781,304	759,470	1,327,243	1,160,288
HTG EdgeSeq | Product revenue
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	1,038,333	1,305,169	1,818,897	1,846,403
HTG EdgeSeq | Custom RUO assay design
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	343,284	1,481,318	972,466	1,849,177
HTG EdgeSeq | RUO Sample processing
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	346,909	1,637,881	925,300	3,391,293
HTG EdgeSeq | Product-related services revenue
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	690,193	3,119,199	1,897,766	5,240,470
HTG EdgeSeq | Product and Product-related Services
Disaggregation Of Revenue [Line Items]
Product and product-related services revenue	$ 1,728,526	$ 4,424,368	$ 3,716,663	$ 7,086,873

Revenue from Contracts with C_4

Revenue from Contracts with Customers - Additional Information (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019	Dec. 31, 2019	Dec. 31, 2018
Disaggregation Of Revenue [Line Items]
Agreements for product and product-related services revenue, description			The Company’s agreements for product and product-related services revenue have an expected duration of one year or less
Revenue recognized			$ 731,875	$ 711,205
Service revenue	$ 1,963,294	$ 5,796,320	4,188,768	8,999,145
Profit sharing payments	974,642	2,508,371	1,990,134	4,553,898
Accounts receivable	1,011,462		1,011,462		$ 3,164,176
Research and development	1,732,776	3,253,639	3,659,051	5,328,387
Contract liabilities	546,172	755,443	546,172	755,443	599,454	$ 410,947
SOW Two
Disaggregation Of Revenue [Line Items]
Accounts receivable	80,465		80,465		171,298
Research and development	200,000	900,000	400,000	1,100,000
SOW Three
Disaggregation Of Revenue [Line Items]
Accounts receivable	0		0		$ 760,274
Research and development		51,871		270,446
Custom RUO Assay Design and Related Agreements
Disaggregation Of Revenue [Line Items]
Revenue recognized	0	0	0	68,820
Collaborative Development Services
Disaggregation Of Revenue [Line Items]
Service revenue	234,768	1,371,952	472,105	1,912,272
Collaborative Development Services | SOW Two
Disaggregation Of Revenue [Line Items]
Service revenue	200,000	1,300,000	500,000	1,600,000
Profit sharing payments	0	300,000	50,000	300,000
Collaborative Development Services | SOW Three
Disaggregation Of Revenue [Line Items]
Service revenue	$ 0	$ 47,668	$ 0	$ 330,289
Minimum
Disaggregation Of Revenue [Line Items]
Customer payment term			30 days
Maximum
Disaggregation Of Revenue [Line Items]
Customer payment term			90 days
Maximum | QIAGEN Manchester Limited
Disaggregation Of Revenue [Line Items]
Term of agreement			1 year

Revenue from Contracts with C_5

Revenue from Contracts with Customers - Additional Information1 (Details) $ in Millions	Jun. 30, 2020USD ($)
Revenue, Remaining Performance Obligation, Expected Timing of Satisfaction, Start Date: 2020-07-01
Revenue Remaining Performance Obligation Expected Timing Of Satisfaction [Line Items]
Revenue, remaining performance obligation, expected timing of satisfaction, period	1 year
Deferred revenue to be realized	$ 0.4
Revenue, Remaining Performance Obligation, Expected Timing of Satisfaction, Start Date: 2021-07-01
Revenue Remaining Performance Obligation Expected Timing Of Satisfaction [Line Items]
Revenue, remaining performance obligation, expected timing of satisfaction, period	1 year
Deferred revenue to be realized	$ 0.1

Revenue from Contracts with C_6

Revenue from Contracts with Customers - Schedule of Collaborative Development Services (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Disaggregation Of Revenue [Line Items]
Service revenue	$ 1,963,294	$ 5,796,320	$ 4,188,768	$ 8,999,145
Collaborative Development Services
Disaggregation Of Revenue [Line Items]
Service revenue	$ 234,768	$ 1,371,952	$ 472,105	$ 1,912,272

Revenue from Contracts with C_7

Revenue from Contracts with Customers - Schedule of Changes in Contract Liability (Details) - USD ($)	6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019
Disaggregation Of Revenue [Line Items]
Beginning Balance	$ 599,454	$ 410,947
Deferral of revenue	678,593	1,055,701
Recognition of deferred revenue	(731,875)	(711,205)
Ending Balance	546,172	755,443
Product Revenue
Disaggregation Of Revenue [Line Items]
Beginning Balance	95,148	116,547
Deferral of revenue	273,829	113,038
Recognition of deferred revenue	(272,653)	(101,419)
Ending Balance	96,324	128,166
Custom RUO Assay Design
Disaggregation Of Revenue [Line Items]
Beginning Balance	66,216	50,000
Deferral of revenue	337,035	466,885
Recognition of deferred revenue	(403,251)	(424,434)
Ending Balance		92,451
Sample Processing
Disaggregation Of Revenue [Line Items]
Beginning Balance	438,090	244,400
Deferral of revenue	67,729	475,778
Recognition of deferred revenue	(55,971)	(185,352)
Ending Balance	$ 449,848	$ 534,826

Other Agreements - Additional I

Other Agreements - Additional Information (Details)	3 Months Ended		6 Months Ended
	Jun. 30, 2020USD ($)	Jun. 30, 2019USD ($)	Jun. 30, 2020USD ($)	Jun. 30, 2019USD ($)	Dec. 31, 2019USD ($)
Other Agreements [Line Items]
Accretion expense			$ (6,656)	$ (6,483)
Measurement Input, Default Rate
Other Agreements [Line Items]
Discount rate used to calculate asset purchase obligation	2.5		2.5
NuvoGen Asset Purchase Agreement
Other Agreements [Line Items]
Asset purchase agreement quarterly installments due from beginning 2019	$ 400,000		$ 400,000
Percentage on annual revenues for cash consideration to be paid			6.00%
Asset purchase agreement fixed quarterly payments	100,000		$ 100,000
Additional revenue based payments payable	17,798		17,798		$ 187,997
NuvoGen
Other Agreements [Line Items]
Convertible notes and related debt discount	(85,655)		(85,655)		$ (92,311)
Accretion expense	$ (3,226)	$ (3,069)	$ (6,656)	$ (6,483)

Other Agreements - Schedule of

Other Agreements - Schedule of Remaining Minimum Principal Payments Due (Details) - NuvoGen Asset Purchase Agreement	Jun. 30, 2020USD ($)
Purchase Obligation Fiscal Year Maturity [Line Items]
2020	$ 442,085
2021	621,525
2022	400,000
2023	400,000
2024	400,000
2025 and beyond	2,873,564
Total NuvoGen obligation payments	5,137,174
Plus interest accretion	85,655
Total NuvoGen obligation, net	$ 5,222,829

Net Loss per Share - Additional

Net Loss per Share - Additional Information (Details) - USD ($)	1 Months Ended	3 Months Ended	6 Months Ended
	Mar. 31, 2020	Mar. 31, 2020	Jun. 30, 2020	Sep. 30, 2019
Class Of Warrant Or Right [Line Items]
Proceeds from exercise of pre-funded warrants			$ 31,768
Pre-funded Warrants
Class Of Warrant Or Right [Line Items]
Exercise price of warrants			$ 0.01
Private Placement | Institutional Accredited Investors
Class Of Warrant Or Right [Line Items]
Exercise price of warrants			$ 0.65	$ 0.65
Private Placement | Institutional Accredited Investors | Pre-funded Warrants
Class Of Warrant Or Right [Line Items]
Issuance of warrants, shares			5,411,687	5,411,687
Number of securities called by warrants			5,411,687	5,411,687
Exercise price of warrants			$ 0.64	$ 0.64
Exercise price of warrants, exercisable subject to beneficial ownership limitations			$ 0.01	$ 0.01
Warrants exercised	3,176,762	3,176,762
Proceeds from exercise of pre-funded warrants	$ 31,768	$ 31,768
Unexercised warrants outstanding			2,234,925

Net Loss per Share - Outstandin

Net Loss per Share - Outstanding Options, Warrants, Restricted Stock Units and Debt Conversion Option Excluded from Computation of Diluted Net Loss per Share (Details) - shares	6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019
Options to purchase common stock
Antidilutive Securities Excluded From Computation Of Earnings Per Share [Line Items]
Anti-dilutive securities excluded from computation of diluted net loss per share	3,990,734	2,334,272
Restricted Stock Units R S U
Antidilutive Securities Excluded From Computation Of Earnings Per Share [Line Items]
Anti-dilutive securities excluded from computation of diluted net loss per share	169,677	254,580
Warrants | Common Stock
Antidilutive Securities Excluded From Computation Of Earnings Per Share [Line Items]
Anti-dilutive securities excluded from computation of diluted net loss per share	880,328	236,915
QNAH convertible note
Antidilutive Securities Excluded From Computation Of Earnings Per Share [Line Items]
Anti-dilutive securities excluded from computation of diluted net loss per share		788,971

Warrants - Additional Informati

Warrants - Additional Information (Details) - USD ($)	1 Months Ended	3 Months Ended	6 Months Ended
	Mar. 31, 2020	Mar. 31, 2020	Jun. 30, 2020	Jun. 24, 2020	Sep. 30, 2019
Class Of Warrant Or Right [Line Items]
Proceeds from exercise of pre-funded warrants			$ 31,768
SVB Term Loan
Class Of Warrant Or Right [Line Items]
Number of securities called by warrants				643,413
Exercise price of warrants			$ 0.7771	$ 0.7771
Warrant to purchase common stock			643,413
Warrant expire date			Jun. 24, 2030
Pre-funded Warrants
Class Of Warrant Or Right [Line Items]
Exercise price of warrants			$ 0.01
Warrant to purchase common stock			2,234,925
Minimum | SVB Term Loan
Class Of Warrant Or Right [Line Items]
Warrant to purchase common stock			643,413
Private Placement | Institutional Accredited Investors
Class Of Warrant Or Right [Line Items]
Exercise price of warrants			$ 0.65		$ 0.65
Private Placement | Institutional Accredited Investors | Pre-funded Warrants
Class Of Warrant Or Right [Line Items]
Issuance of warrants, shares			5,411,687		5,411,687
Number of securities called by warrants			5,411,687		5,411,687
Exercise price of warrants			$ 0.64		$ 0.64
Exercise price of warrants, exercisable subject to beneficial ownership limitations			$ 0.01		$ 0.01
Proceeds from exercise of pre-funded warrants	$ 31,768	$ 31,768
Warrants exercised	3,176,762	3,176,762

Warrants -Summary of Outstandin

Warrants -Summary of Outstanding Warrants (Details) - $ / shares	6 Months Ended
	Jun. 30, 2020	Jun. 24, 2020
SVB Term Loan
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2020-06
Shares of Common Stock Underlying Warrants	643,413
Exercise Price/Share	$ 0.7771	$ 0.7771
Expiration Date	2030
Convertible Note Warrants
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2014-12
Shares of Common Stock Underlying Warrants	144,772
Exercise Price/Share	$ 14
Expiration Date	2022
Common Stock Warrants
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2016-03
Shares of Common Stock Underlying Warrants	45,307
Exercise Price/Share	$ 2.76
Expiration Date	2026
Common Stock Tranche one
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2018-03
Shares of Common Stock Underlying Warrants	18,123
Exercise Price/Share	$ 7.73
Expiration Date	2028
Pre-funded Warrants
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2019-09
Shares of Common Stock Underlying Warrants	2,234,925
Exercise Price/Share	$ 0.01
Series E Redeemable Convertible Preferred Stock
Class Of Warrant Or Right [Line Items]
Warrant Issuance Date	2014-08
Shares of Common Stock Underlying Warrants	28,713
Exercise Price/Share	$ 23.51
Expiration Date	2024

Stockholders Deficit - Addition

Stockholders Deficit - Additional Information (Details) - USD ($)	Mar. 24, 2020	Feb. 25, 2020	Jun. 30, 2020	Mar. 31, 2020	Sep. 30, 2019	Jun. 30, 2020	Mar. 31, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019	Dec. 31, 2019	Nov. 30, 2019
Class Of Stock [Line Items]
Common stock, shares outstanding			67,093,683			67,093,683			67,093,683		58,090,233
Common stock, shares issued			67,093,683			67,093,683			67,093,683		58,090,233
Employee Stock Option
Class Of Stock [Line Items]
Unrecognized compensation expense			$ 1,800,000			$ 1,800,000			$ 1,800,000
Compensation expense period expected to be recognized									1 year 9 months 3 days
Restricted Stock Units R S U
Class Of Stock [Line Items]
Unrecognized compensation expense			$ 400,000			$ 400,000			$ 400,000
Compensation expense period expected to be recognized									1 year 10 months 13 days
Vested and unissued RSU awards, description									Vested and unissued awards at June 30, 2020 represents RSU awards for which the vesting date was June 30, 2020, but for which issuance of the awards occurred in July 2020.
Vested and unissued RSU awards vesting date									Jun. 30, 2020
Vested and unissued RSU awards issuance date									Jul. 31, 2020
Common Stock
Class Of Stock [Line Items]
Number of shares issued upon the conversion						2,750,000
Cantor Sales Agreement
Class Of Stock [Line Items]
Common stock price per share												$ 0.001
Exchange Agreement
Class Of Stock [Line Items]
Common stock, shares outstanding		4,110,000
Conversion of convertible preferred stock into common stock		100
Exchange Agreement | Series A Convertible Preferred Stock
Class Of Stock [Line Items]
Issuance of common stock, shares		10,170
Preferred stock, shares issued		41,100
Preferred stock, par value		$ 0.001
Issuance of common stock		$ 600,030
Stock issued, price per share		$ 59
Preferred stock, shares converted			27,500
Preferred stock, shares outstanding			23,770			23,770			23,770
Exchange Agreement | Common Stock
Class Of Stock [Line Items]
Number of shares issued upon the conversion			2,750,000
LP Purchase Agreement | Common Stock
Class Of Stock [Line Items]
Issuance of common stock, shares							615,384
Issuance of common stock							$ 615
Pre-funded Warrants
Class Of Stock [Line Items]
Exercise price of warrants			$ 0.01			$ 0.01			$ 0.01
Lincoln Park | LP Purchase Agreement
Class Of Stock [Line Items]
Common stock value right to sell, maximum	$ 20,000,000
Agreement term	36 months
Common stock, shares issued	615,384		0			0			0
Lincoln Park | LP Purchase Agreement | Selling, General and Administrative Expense
Class Of Stock [Line Items]
Commitment consideration and related transaction costs						$ 100,000		$ 0	$ 100,000	$ 0
Private Placement | Institutional Accredited Investors
Class Of Stock [Line Items]
Exercise price of warrants			$ 0.65		$ 0.65	$ 0.65			$ 0.65
Private Placement | Institutional Accredited Investors | Pre-funded Warrants
Class Of Stock [Line Items]
Issuance of warrants, shares			5,411,687		5,411,687	5,411,687			5,411,687
Number of securities called by warrants			5,411,687		5,411,687	5,411,687			5,411,687
Exercise price of warrants			$ 0.64		$ 0.64	$ 0.64			$ 0.64
Exercise price of warrants, exercisable subject to beneficial ownership limitations			$ 0.01		$ 0.01	$ 0.01			$ 0.01
Private Placement | Cantor Fitzgerald & Co. | Pre-funded Warrants
Class Of Stock [Line Items]
Number of securities called by warrants				3,176,762			3,176,762
Agent cash fee, percentage of gross proceeds from sale of warrants					6.00%
Net proceeds from sale of warrants				$ 31,768	$ 3,100,000
ATM Offering
Class Of Stock [Line Items]
Gross proceeds from issuance of common stock									$ 4,000,000
Payment of sales commissions									100,000
Net proceeds from issuance of common stock from the ATM Offering									$ 3,800,000
Issuance of common stock						$ 1,395,058	$ 2,435,912
ATM Offering | Common Stock
Class Of Stock [Line Items]
Issuance of common stock, shares						2,084,714	4,399,062		6,483,776
Deferred offering costs											$ 100,000
Issuance of common stock						$ 2,085	$ 4,399

Stockholders Deficit - Summary

Stockholders Deficit - Summary of Stock Option Plans Activity (Details) - Employee Stock Option - USD ($)	6 Months Ended	12 Months Ended
	Jun. 30, 2020	Dec. 31, 2019
Share Based Compensation Arrangement By Share Based Payment Award [Line Items]
Number of Shares, Beginning Balance	2,904,898
Number of Shares, Granted	1,347,500
Number of Shares, Forfeited	(212,860)
Number of Shares, Expired/Cancelled	(48,804)
Number of Shares, Ending Balance	3,990,734	2,904,898
Number of Shares, Exercisable	2,046,557
Weighted-Average Exercise Price Per Share, Beginning Balance	$ 2.37
Weighted-Average Exercise Price Per Share, Granted	0.69
Weighted-Average Exercise Price Per Share, Forfeited	1.79
Weighted-Average Exercise Price Per Share, Expired/Cancelled	3.42
Weighted-Average Exercise Price Per Share, Ending Balance	1.82	$ 2.37
Weighted-Average Exercise Price Per Share, Exercisable	$ 2.27
Weighted-Average Remaining Contractual Life, Outstanding	8 years 1 month 6 days	8 years
Weighted-Average Remaining Contractual Life, Exercisable	7 years
Aggregate Intrinsic Value, Balance	$ 54,750	$ 781
Aggregate Intrinsic Value, Exercisable	$ 13,038

Stockholders Deficit - Summar_2

Stockholders Deficit - Summary of Restricted Stock Unit ('RSU') Award Activity (Details) - Restricted Stock Units R S U	6 Months Ended
	Jun. 30, 2020$ / sharesshares
Restricted Stock Units (RSU)
Beginning Balance | shares	223,745
Granted | shares	22,500
Released | shares	(59,693)
Forfeited | shares	(16,875)
Ending Balance | shares	169,677
Vested and unissued at June 30, 2020 | shares	23,126
Weighted Average Grant Date Fair Value Per Share
Beginning Balance | $ / shares	$ 2.91
Granted | $ / shares	0.75
Released | $ / shares	2.66
Forfeited | $ / shares	3.04
Ending Balance | $ / shares	2.70
Vested and unissued at June 30, 2020 | $ / shares	$ 2.73

Stockholders Deficit - Summar_3

Stockholders Deficit - Summary of Stock-Based Compensation Recorded in the Condensed Consolidated Statements of Operations (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Employee Service Share Based Compensation Allocation Of Recognized Period Costs [Line Items]
Stock-based compensation	$ 366,880	$ 287,332	$ 787,551	$ 528,578
Selling, General and Administrative
Employee Service Share Based Compensation Allocation Of Recognized Period Costs [Line Items]
Stock-based compensation	338,063	217,195	696,075	399,176
Research and Development
Employee Service Share Based Compensation Allocation Of Recognized Period Costs [Line Items]
Stock-based compensation	24,913	58,234	83,418	106,684
Cost of Product and Product-related Services Revenue
Employee Service Share Based Compensation Allocation Of Recognized Period Costs [Line Items]
Stock-based compensation	$ 3,904	$ 11,903	$ 8,058	$ 22,718

Leases - Additional Information

Leases - Additional Information (Details) - USD ($)	6 Months Ended
	Jun. 30, 2020	Dec. 31, 2019
Lessee Lease Description [Line Items]
Lease expiration year	2023
Operating lease, description	The Company’s most significant active leases as of June 30, 2020 are for office and manufacturing space in Tucson, Arizona
Operating lease right-of-use assets	$ 905,459	$ 1,209,145
Financing lease liabilities, net of discount	88,963
Financing lease liabilities, current	$ 30,983
Finance Lease, Liability, Current, Statement of Financial Position [Extensible List]	us-gaap:OtherLiabilitiesCurrent
Financing lease liabilities, non current	$ 57,980
Finance Lease, Liability, Noncurrent, Statement of Financial Position [Extensible List]	us-gaap:OtherLiabilitiesNoncurrent
Financing leases, right-of-use assets	$ 85,598
Tucson, Arizona
Lessee Lease Description [Line Items]
Lease expiration year	2021
San Carlos, California
Lessee Lease Description [Line Items]
Lease expiration year	2023
Sausheim, France | Selling, General and Administrative Expense
Lessee Lease Description [Line Items]
Operating lease right-of-use assets	$ 21,096

Leases - Summary of Components

Leases - Summary of Components of Lease Cost for Operating Leases (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Leases [Abstract]
Operating lease cost	$ 182,649	$ 164,550	$ 387,876	$ 260,731
Variable lease cost	43,706	31,807	53,162	53,258
Operating lease expense	226,355	196,357	441,038	313,989
Short-term lease rent expense				1,497
Total rent expense	$ 226,355	$ 196,357	$ 441,038	$ 315,486

Leases - Summary of Other Infor

Leases - Summary of Other Information Related to Operating Leases (Details) - USD ($)	6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019
Leases [Abstract]
Operating cash flows for operating leases	$ 439,518	$ 338,089
Establishment of operating lease liabilities arising from obtaining right-of-use assets	$ 21,078	$ 1,927,652
Weighted-average remaining lease term – operating leases	2 years	2 years 8 months 12 days
Weighted-average discount rate – operating leases	9.60%	9.60%

Leases - Summary of Remaining M

Leases - Summary of Remaining Maturities of Operating Leases, Excluding Short-term Leases (Details)	Jun. 30, 2020USD ($)
Leases [Abstract]
2020	$ 441,781
2021	352,243
2022	281,232
2023	70,848
Total	1,146,104
Less present value discount	(108,628)
Operating lease liabilities, net	$ 1,037,476

Leases - Summary of Component_2

Leases - Summary of Components of Lease Cost for Financing Leases (Details) - USD ($)	3 Months Ended		6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019	Jun. 30, 2020	Jun. 30, 2019
Leases [Abstract]
Amortization of right-of-use assets	$ 11,480	$ 12,084	$ 23,998	$ 26,780
Interest on lease liability	2,347	1,823	4,982	3,969
Total financing lease cost	$ 13,827	$ 13,907	$ 28,980	$ 30,749

Leases - Summary of Other Inf_2

Leases - Summary of Other Information Related to Financing Leases (Details)	Jun. 30, 2020	Jun. 30, 2019
Leases [Abstract]
Weighted-average remaining lease term – financing leases	3 years 7 months 6 days	2 years 2 months 12 days
Weighted-average discount rate – financing leases	9.77%	9.77%

Leases - Summary of Remaining_2

Leases - Summary of Remaining Maturities of Financing Leases (Details)	Jun. 30, 2020USD ($)
Leases [Abstract]
2020	$ 22,245
2021	28,355
2022	20,716
2023	18,396
2024	16,080
Total	105,792
Less present value discount	(16,829)
Financing lease liabilities, net	$ 88,963

Commitments and Contingencies -

Commitments and Contingencies - Summary Of Product Warranty Liability (Details) - USD ($)	6 Months Ended
	Jun. 30, 2020	Jun. 30, 2019
Commitments And Contingencies Disclosure [Abstract]
Beginning balance	$ 94,482	$ 63,461
Cost of warranty claims	(12,056)	(36,434)
Increase in warranty reserve	7,964	47,612
Ending balance	$ 90,390	$ 74,639

Subsequent Events - Additional

Subsequent Events - Additional Information (Details) - Subsequent Event $ in Millions	1 Months Ended
	Aug. 07, 2020USD ($)shares
Subsequent Event [Line Items]
Gross proceeds from issuance of common stock	$ 2.4
Net proceeds from issuance of common stock from the ATM Offering	$ 2.3
Common Stock
Subsequent Event [Line Items]
Issuance of common stock, shares | shares	3,594,196