and addresses a wide range of disabilities including dyslexia, color blindness, epilepsy and more. AudioEye also offers additional solutions to provide for enhanced compliance and accessibility, including periodic manual auditing, manual remediations and legal support services. Our solutions may be purchased through a subscription service on a month-to-month basis or with one or multi-year terms. We also offer PDF remediation services and Native Mobile App and Audit reports to help our customers with their digital accessibility needs.
Intellectual Property
Our intellectual property is primarily comprised of copyrights, trademarks, trade secrets, issued patents and pending patent applications. We have a patent portfolio comprised of twenty-four (24) issued patents in the United States and three (3) pending US patent applications. The commercial value of these patents is unknown.
We plan to continue to invest in research and development and expand our portfolio of proprietary intellectual property.
Our Annual Report filed on Form 10-K for the year ended December 31, 2022 as filed with the SEC on March 9, 2023 provides additional information about our business and operations.
Executive Overview
AudioEye is an industry-leading digital accessibility platform delivering ADA and WCAG compliance at scale. Our solutions advance accessibility with patented technology that reduces barriers, expands access for individuals with disabilities, and enhances the user experience for a broader audience. In the first quarter of 2023, we saw continued revenue growth, increases in customer count, and expansion in our product features and capabilites.
We have two sales channels to deliver our product, the Partner and Marketplace channel and the Enterprise channel. AudioEye continues to focus on recurring revenue growth in both channels, while still offering our Website and Native Mobile App report services and PDF services. For the three months ended March 31, 2023, total revenue increased by 13% over the prior year comparable period. As of March 31, 2023, Annual Recurring Revenue (“ARR”) was approximately $29.6 million, which represented an increase of 5% year-over-year. Refer to Other Key Operating Metrics below for details on how we calculate ARR.
As of March 31, 2023, AudioEye had approximately 95,000 customers, an increase from 74,000 customers at March 31, 2022. Customer count increased in both the Enterprise and Partner and Marketplace channel during this period.
In the three months ended March 31, 2023, revenue from our Partner and Marketplace grew 14% from prior year comparable period. This channel represented about 59% of ARR at the end of March 2023. In three months ended March 31, 2023, total Enterprise revenue grew by 11% from prior year comparable period. The Enterprise channel represented about 41% of ARR at the end of March 2023.
In the three months ended March 31, 2023 and 2022, we had one customer (including the customer’s affiliates reflecting multiple contracts and a partnership with the Company) which accounted for approximately 16% and 18%, respectively, of our total revenue.
The Company continued to invest in Research and Development in the first quarter of 2023. Total Research and Development cost, as defined under Research and Development section in the Results of Operations below, was 29% of total revenue in the first quarter of 2023. Total research and development cost increased primarily due to additional investments in engineering and product talent.
While revenue increased 13% in the three months ended March 31, 2023, both Sales and Marketing expense and General and Administrative expense decreased from prior year comparable period. This decrease was mainly driven by efficiencies implemented during the year in these areas and was partially offset by increased costs associated with BOIA and with other expenses.
We provide further commentary on our Results of Operation below.