and addresses a wide range of disabilities including dyslexia, color blindness, epilepsy and more. AudioEye also offers additional solutions to provide for enhanced compliance and accessibility, including periodic manual auditing, manual remediations and legal support services. Our solutions may be purchased through a subscription service on a month-to-month basis or with one or multi-year terms. We also offer PDF remediation services and Website and Native Mobile App reports to help our customers with their digital accessibility needs.
Intellectual Property
Our intellectual property is primarily comprised of copyrights, trademarks, trade secrets, issued patents and pending patent applications. We have a patent portfolio comprised of twenty-one (21) issued patents in the United States and three (3) pending US patent applications. The commercial value of these patents is unknown.
We plan to continue to invest in research and development and expand our portfolio of proprietary intellectual property.
Our Annual Report filed on Form 10-K for the year ended December 31, 2022 as filed with the SEC on March 9, 2023 provides additional information about our business and operations.
Executive Overview
AudioEye is an industry-leading digital accessibility platform delivering ADA and WCAG compliance at scale. Our solutions advance accessibility with patented technology that reduces barriers, expands access for individuals with disabilities, and enhances the user experience for a broader audience. In the three and nine months ended September 30, 2023, we saw continued revenue growth, increases in customer count, efficiencies in cost of revenue that increased gross margins and decreased total operating expenses.
We have two sales channels to deliver our product, the Partner and Marketplace channel and the Enterprise channel. AudioEye continues to focus on recurring revenue growth in both channels, while still offering our Website and Native Mobile App report services and PDF services. For the nine months ended September 30, 2023, total revenue increased by 6% over the prior year comparable period. As of September 30, 2023, Annual Recurring Revenue (“ARR”) was approximately $30.5 million, which represented an increase of 4% year-over-year. Refer to Other Key Operating Metrics below for details on how we calculate ARR.
As of September 30, 2023, AudioEye had approximately 107,000 customers, a 32% increase from 81,000 customers at September 30, 2022. The increase in customer count is attributed to our Partner and Marketplace channel.
In the nine months ended September 30, 2023, revenue from our Partner and Marketplace grew 14% from prior year comparable period. This channel represented about 61% of ARR at the end of September 2023. In nine months ended September 30, 2023, total Enterprise revenue decreased by 3% from prior year comparable period. The Enterprise channel represented about 39% of ARR at the end of September 2023.
In the three and nine months ended September 30, 2023, we had one customer (including the customer’s affiliates reflecting multiple contracts and a partnership with the Company) which accounted for approximately 17% and 16%, respectively, of our total revenue. In the three and nine months ended September 30, 2022, we had one customer which accounted for approximately 16% and 17%, respectively, of our total revenue.
The Company continued to invest in research and development in the third quarter of 2023. Total research and development cost, as defined under Research and Development Expenses section in the Results of Operations below, was 31% of total revenue in the three months ended September 30, 2023. Total research and development cost increased primarily due to additional investments in engineering and product talent dedicated to further product development and enhancement.
While revenue increased 6% in the nine months ended September 30, 2023, cost of revenue, selling and marketing expense and general and administrative expense decreased from prior year comparable period. This decrease was mainly driven by efficiencies in cost of revenue and selling and marketing implemented during the year, and lower litigation expense.
We provide further commentary on our Results of Operation below.