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Exhibit 99.1

PROSTONES AS ClC-2 Cl- ClC Cl CHANNEL ACTIVATORS FOR TREATMENT OF DISEASES AND DISORDERS John Cuppoletti University of Cincinnati, Cincinnati,OH and Ryuji Ueno, Sucampo Pharmaceuticals Inc., Bethesda, MD

Prostones · Prostones are derivatives of metabolites of prostaglandins. · Lubiprostone is a prostone which is used to clinically to treat chronic idiopathic constipation and irritable bowel syndrome related to constipation (IBS-C). · Lubiprostone is a ClC-2 chloride channel activator which increases salt and water secretion in the intestine.

ClC Cl^-CHANNEL IS THE TARGET FOR LUBIPROSTONE ClC Cl^-CHANNELS ARE DIMERSMEMBRANE DOMAIN¹ CYTOSOLIC C- TERMINAL DOMAIN²¹R.Dutzler, E.B. Campbell, M. Cadene, B.T. Chait and R.MacKinnon Nature 415, 287-294(2002)
²S. Alioth, S. Meyer, R. Dutzler and K. PervushinJournal of Molecular BiologyVol. 369, Issue 5, 22 June 2007, Pages 1163-1169

LUBIPROSTONE POTENTLY ACTIVATES RECOMBINANT HUMAN ClC-2 CHLORIDE CHANNELS STUDIED BY WHOLE CELL PATCH CLAMP Cuppoletti, J. et al. Am J Physiol Cell Physiol 287: C1173-C1183 2004^©2004 American Physiological Society

LUBIPROSTONE ACTIVATES RECOMBINANT HUMAN ClC-2 Cl^-CHANNELS IN HEK293 CELLS AT SINGLE CHANNEL LEVEL RECORDED BY PATCH CLAMP

siRNA TO ClC-2 ABLATES LUBIPROSTONE ACTIVATION OF CHLORIDE TRANSPORT BY RECOMBINANT HUMAN ClC-2 IN HEK293 CELLS Cuppoletti J, Malinowska DH Tewari K, Chakrabarti J, Ueno RGastroenterology, Vol. 134, Issue: 4, April, 2008. pp. A-582

LUBIPROSTONE ACTIVATES Cl^-TRANSPORT IN T84 INTESTINAL CELL CULTURES STUDIED BY SHORT CIRCUIT CURRENT WITH USSING CHAMBERS USSING CHAMBER
Cuppoletti, J. et al. Am J Physiol Cell Physiol 287: C1173-C1183 2004

LUBIPROSTONE POTENTLY STIMULATES Cl^-TRANSPORT IN T84 INTESTINAL CELLSCuppoletti, J. et al. Am J Physiol Cell Physiol 287: C1173-C1183 2004

siRNA TO ClC-2 ABLATES STIMULATION OF Cl^-TRANSPORT BY LUBIPROSTONE IN T84 CELLS Cuppoletti J, Malinowska DH Tewari K, Chakrabarti J, Ueno RGastroenterology, Vol. 134, Issue: 4, April, 2008. pp. A-582

ClC-2 IS EXPRESSED ON THE APICAL MEMBRANE AS SHOWN USING NYSTATIN SOLUBILIZATION OF APICAL OR BASOLATERAL MEMBRANECuppoletti, J. et al. Am J Physiol Cell Physiol 287: C1173-C1183 2004

LUBIPROSTONE DOES NOT STIMULATE Cl^-TRANSPORT BY CFTRCuppoletti, J. et al. Am J Physiol Cell Physiol 287: C1173-C1183 2004

LUBIPROSTONE DOES NOT ACT THROUGH INCREASES IN INTRACELLULAR CALCIUM Cuppoletti J, Malinowska DH Tewari K, Chakrabarti J, Ueno RGastroenterology, Vol. 134, Issue: 4, April, 2008. pp. A581-A-582

LUBIPROSTONE DOES NOT ACTIVATE Cl^-TRANSPORT THROUGH EP OR FP RECEPTORS J.Cuppoletti, D.H.Malinowska, R.Ueno. ACG meeting, Orlando,Fl October 5, 2008

LUBIPROSTONE PROMOTES REPAIR OF BARRIER FUNCTION AFTER DAMAGE BY INDOMETHACIN AND ISCHEMIA Moeser, A. J. et al. Am J Physiol Gastrointest Liver Physiol 292: G647-G656 2007;

SUMMARY OF LUBIPROSTONE MECHANISMS OF ACTION · LUBIPROSTONE TARGETS ClC-2 CHLORIDE CHANNELS IN THE INTESTINE AS SHOWN BY WHOLE CELL CURRENTS, SINGLE CHANNELS AND SHORT CIRCUIT CURRENT. · LUBIPROSTONE POTENTLY ACTIVATES ClC-2 CHLORIDE CHANNELS · LUBIPROSTONE DOES NOT AFFECT OTHER INTESTINAL CHLORIDE CHANNELS · THE SITE OF ACTION OF LUBIPROSTONE IS THE APICAL MEMBRANE · LUBIPROSTONE DOES NOT ACT THROUGH EP- OR FP-RECEPTORS, INCREASES IN cAMP, OR INCREASED CALCIUM · LUBIPROSTONE INCREASES SALT AND WATER SECRETION IN INTESTINAL CELLS AND THIS IS THE BASIS FOR CLINICAL ACTION IN CONSTIPATION · LUBIPROSTONE PROMOTES REPAIR OF EPITHELIAL BARRIERS AND THIS MAY CONTRIBUTE TO CLINICAL ACTION IN IBS-C

ACKNOWLEGDEMENT · THESE STUDIES WERE SUPPORTED BY A GRANT FROM SUCAMPO PHARMACEUTICALS, INC., BETHESDA MD, USA