Exhibit 32.1
CERTIFICATION PURSUANT TO
18 U.S.C. SECTION 1350,
AS ADOPTED PURSUANT TO
SECTION 906 OF THE SARBANES-OXLEY ACT OF 2002
In connection with the Annual Report onForm 10-K of Zafgen, Inc. (the “Company”) for the period ended December 31, 2016, as filed with the Securities and Exchange Commission on the date hereof (the “Report”), each of the undersigned officers hereby certify, pursuant to Section 906 of the Sarbanes-Oxley Act of 2002, 18 U.S.C. Section 1350, that to his or her knowledge:
| 1) | the Report which this statement accompanies fully complies with the requirements of Section 13(a) or 15(d) of the Securities Exchange Act of 1934, as amended; and |
| 2) | the information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Company. |
Dated: March 10, 2017 | /s/ Thomas E. Hughes | |
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| Thomas E. Hughes | ||
| President and Chief Executive Officer | ||
| (Principal Executive Officer) | ||
Dated: March 10, 2017 | /s/ Patricia Allen | |
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| Patricia Allen | ||
| Chief Financial Officer | ||
(Principal Financial and Accounting Officer) | ||