Exhibit 23.1
Consent of Independent Registered Public Accounting Firm
We consent to the reference to our firm under the caption “Experts” in the Registration Statement (Form S-3) and related Prospectus of Assembly Biosciences, Inc. for the registration of common stock, preferred stock, debt securities, warrants, subscription rights and units, and to the incorporation by reference therein of our report dated March 22, 2023, with respect to the consolidated financial statements of Assembly Biosciences, Inc., included in its Annual Report (Form 10-K) for the year ended December 31, 2022, filed with the Securities and Exchange Commission.
/s/ Ernst & Young LLP
San Jose, California
March 22, 2023
