SandRidge Mississippian Trust I (SDTTU) 10-Q 2016 Q1 Quarterly report

Exhibit 32.1

Securities and Exchange Commission

100 F Street, N.E.

Washington, D.C. 20549

RE:Certification pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 (18 U.S.C. Section 1350)

Ladies and Gentlemen:

In connection with the Quarterly Report of SandRidge Mississippian Trust I (the “Trust”) on Form 10-Q for the quarterly period ended March 31, 2016 as filed with the Securities and Exchange Commission on the date hereof (the “Report”), the undersigned, not in its individual capacity but solely as the Trustee of the Trust, certifies pursuant to 18 U.S.C. § 1350, that to its knowledge:

(1)The Report fully complies with the requirements of Section 13(a) or 15(d), as applicable, of the Securities Exchange Act of 1934, as amended; and

(2)The information contained in the Report fairly presents, in all material respects, the financial condition and results of operations of the Trust.

The above certification is furnished solely pursuant to Section 906 of the Sarbanes-Oxley Act of 2002 (18 U.S.C. 1350) and is not being filed as part of the Report or as a separate disclosure document.

/s/ Sarah Newell

Sarah Newell

Vice President

The Bank of New York Mellon Trust Company, N.A.,

as Trustee of SandRidge Mississippian Trust I

May 10, 2016