Ascendis Pharma A/S (ASND) SC 13G Ascendis Pharma A/S

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

SCHEDULE 13G

Under the Securities Exchange Act of 1934
(Amendment No.     )*

Ascendis Pharma A/S

(Name of Issuer)

Ordinary Shares

(Title of Class of Securities)

04351P101

(CUSIP Number)

December 31, 2015

(Date of Event Which Requires Filing of this Statement)

Check the appropriate box to designate the rule pursuant to which this Schedule is filed:

o Rule 13d-1(b)

o Rule 13d-1(c)

x Rule 13d-1(d)

*The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page.

The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes).

CUSIP No. 04351P101						Schedule 13G	Page 2 of 12 Pages
	1.	Names of Reporting Persons Gilde Healthcare Holding BV
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) OO

CUSIP No. 04351P101						Schedule 13G	Page 3 of 12 Pages
	1.	Names of Reporting Persons Gilde Healthcare II Sub-Holding B.V.
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) OO

CUSIP No. 04351P101						Schedule 13G	Page 4 of 12 Pages
	1.	Names of Reporting Persons Gilde Healthcare II Management BV
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) OO

CUSIP No. 04351P101						Schedule 13G	Page 5 of 12 Pages
	1.	Names of Reporting Persons Marc Olivier Perret
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) IN

CUSIP No. 04351P101						Schedule 13G	Page 6 of 12 Pages
	1.	Names of Reporting Persons Edwin de Graaf
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) IN

CUSIP No. 04351P101						Schedule 13G	Page 7 of 12 Pages
	1.	Names of Reporting Persons Martemanshurk BV (100% owned by Pieter van der Meer)
	2.	Check the Appropriate Box if a Member of a Group (See Instructions)
		(a)		o
		(b)		o
	3.	SEC Use Only
	4.	Citizenship or Place of Organization The Netherlands
Number of Shares Beneficially Owned by Each Reporting Person With			5.		Sole Voting Power 0
			6.		Shared Voting Power 1,859,136
			7.		Sole Dispositive Power 0
			8.		Shared Dispositive Power 1,859,136
	9.	Aggregate Amount Beneficially Owned by Each Reporting Person 1,859,136
	10.	Check if the Aggregate Amount in Row (9) Excludes Certain Shares (See Instructions)  o
	11.	Percent of Class Represented by Amount in Row (9) 7.4%
	12.	Type of Reporting Person (See Instructions) OO

CUSIP No. 04351P101 Schedule 13G Page 8 of 12 Pages

Item 1.
	(a)	Name of Issuer: Ascendis Pharma A/S
	(b)	Address of the Issuer’s Principal Executive Offices: Tuborg Boulevard 12 DK-2900 Hellerup, Denmark
Item 2.
	(a)	Name of Person Filing: Gilde Healthcare Holding BV Gilde Healthcare II Sub-Holding BV Gilde Healthcare II Management BV Marc Olivier Perret Edwin de Graaf Martemanshurk BV (100% owned by Pieter van der Meer)
	(b)	Address of Principal Business Office or if none, Residence: c/o Gilde Healthcare Partners BV Newtonlaan 91 3584 BP Utrecht The Netherlands
	(c)	Citizenship: Each of the reporting persons is organized and based in the Netherlands
	(d)	Title and Class of Securities: Ordinary Shares, DKK 1 nominal value per share
	(e)	CUSIP Number: 04351P101
Item 3.
Not applicable.

CUSIP No. 04351P101 Schedule 13G Page 9 of 12 Pages

Item 4.	Ownership:
Provide the following information regarding the aggregate number and percentage of the class of securities of the Issuer identified in Item 1.
	(a)	Amount beneficially owned:

		Gilde Healthcare Holding BV		1,859,136 shares
		Gilde Healthcare II Sub-Holding BV		1,859,136 shares
		Gilde Healthcare II Management BV		1,859,136 shares
		Marc Olivier Perret		1,859,136 shares
		Edwin de Graaf		1,859,136 shares
		Martemanshurk BV (100% owned by Pieter van der Meer)		1,859,136 shares
		Gilde Healthcare II Sub-Holding BV directly owns 1,859,136 ordinary shares of the Issuer (the “Shares). The manager of Gilde Healthcare II Sub-Holding BV is Gilde Healthcare II Management BV which is owned by Gilde Healthcare Holding BV. The three managing partners of Gilde Healthcare Holding BV are Edwin de Graaf, Marc Olivier Perret and Martemanshurk BV (of which Pieter van der Meer is the owner and manager).
		Each of Messrs. Perret, De Graaf, van der Meer and Martemanshurk BV disclaim beneficial ownership of the Shares except to the extent of his or its pecuniary interest therein.
	(b)	Percent of class:
		Gilde Healthcare Holding BV			7.4%
		Gilde Healthcare II Sub-Holding BV			7.4%
		Gilde Healthcare II Management BV			7.4%
		Marc Olivier Perret			7.4%
		Edwin de Graaf			7.4%
		Martemanshurk BV (100% owned by Pieter van der Meer)			7.4%
	(c)	Number of shares as to which the person has:
		(i)	Sole power to vote or to direct the vote:
			Gilde Healthcare Holding BV		0 shares
			Gilde Healthcare II Sub-Holding BV		0 shares
			Gilde Healthcare II Management BV		0 shares
			Marc Olivier Perret		0 shares
			Edwin de Graaf		0 shares
			Martemanshurk BV (100% owned by Pieter van der Meer)		0 shares
		(ii)	Shared power to vote or to direct the vote:
			Gilde Healthcare Holding BV		1,859,136 shares
			Gilde Healthcare II Sub-Holding BV		1,859,136 shares
			Gilde Healthcare II Management BV		1,859,136 shares
			Marc Olivier Perret		1,859,136 shares
			Edwin de Graaf		1,859,136 shares
			Martemanshurk BV (100% owned by Pieter van der Meer)		1,859,136 shares
		(iii)	Sole power to dispose or to direct the disposition of:
			Gilde Healthcare Holding BV		0 shares
			Gilde Healthcare II Sub-Holding BV		0 shares
			Gilde Healthcare II Management BV		0 shares
			Marc Olivier Perret		0 shares
			Edwin de Graaf		0 shares
			Martemanshurk BV (100% owned by Pieter van der Meer)		0 shares
		(iv)	Shared power to dispose or to direct the disposition of:
			Gilde Healthcare Holding BV		1,859,136 shares
			Gilde Healthcare II Sub-Holding BV		1,859,136 shares
			Gilde Healthcare II Management BV		1,859,136 shares
			Marc Olivier Perret		1,859,136 shares
			Edwin de Graaf		1,859,136 shares
			Martemanshurk BV (100% owned by Pieter van der Meer)		1,859,136 shares
Item 5.	Ownership of Five Percent or Less of a Class:
If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than 5 percent of the class of securities, check the following   o.
Item 6.	Ownership of More than Five Percent on Behalf of Another Person:
Not Applicable
Item 7.	Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on By the Parent Holding Company or Control Person:
Not Applicable
Item 8.	Identification and Classification of Members of the Group:
Item 9.	Notice of Dissolution of Group:
Not Applicable

CUSIP No. 04351P101 Schedule 13G Page 10 of 12 Pages

Item 10.	Certifications:
Not Applicable

SIGNATURE

After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct.

Gilde Healthcare Holding BV
By:	/s/ / Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
Gilde Healthcare II Sub-Holding BV
By:	/s/ / Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
Gilde Healthcare II Management BV
By:	/s/ Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
By:	/s/ / Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
					By:	Pieter van der Meer
					Title:	Managing Partner
February 8, 2016

CUSIP No. 04351P101 Schedule 13G Page 11 of 12 Pages

EXHIBIT INDEX

Exhibit 1Joint Filing Agreement as required by Rule 13d-1(k)(1) under the Securities Exchange Act of 1934, as amended.

CUSIP No. 04351P101 Schedule 13G Page 12 of 12 Pages

Exhibit 1

JOINT FILING AGREEMENT PURSUANT TO RULE 13d-1(k)(1)

The undersigned acknowledge and agree that the foregoing statement on Schedule 13G is filed on behalf of each of the undersigned and that all subsequent amendments to this statement on Schedule 13G shall be filed on behalf of each of the undersigned without the necessity of filing additional joint filing statements. The undersigned acknowledge that each shall be responsible for the timely filing of such amendments, and for the completeness and accuracy of the information concerning him, her or it contained herein, but shall not be responsible for the completeness and accuracy of the information concerning the other entities or persons, except to the extent that he, she or it knows or has reason to believe that such information is inaccurate.

Date: February 8, 2016
Gilde Healthcare Holding BV
By:	/s/ Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
Gilde Healthcare II Sub-Holding BV
By:	/s/ Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
Gilde Healthcare II Management BV
By:	/s/ / Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
Title:	Managing Partner	Title:	Managing Partner		By:	Pieter van der Meer
					Title:	Managing Partner
*****
By:	/s/ / Marc Olivier Perret	By:	/s/ Edwin de Graaf	By:	/s/ Pieter van der Meer
Name:	Marc Olivier Perret	Name:	Edwin de Graaf	Name:	Martemanshurk BV
					By:	Pieter van der Meer
					Title:	Managing Partner