Cellectis (CLLS) F-3ASR Automatic shelf registration (foreign)

Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We consent to the reference to our firm under the caption “Experts” in the Registration Statement (Form F-3) and related Prospectus of Cellectis S.A. for the registration of an indeterminate number of ordinary shares, preferred shares and warrants, and to the incorporation by reference therein of our reports dated March 4, 2020, with respect to the consolidated financial statements of Cellectis S.A. and the effectiveness of internal control over financial reporting of Cellectis S.A., included in its Annual Report (Form 20-F) for the year ended December 31, 2019, filed with the Securities and Exchange Commission.

/S/ ERNST & YOUNGET AUTRES

Paris La Défense, France

June 2, 2020