Exhibit 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We hereby consent to the incorporation by reference in this Registration Statement onForm S-3 of our report dated March 23, 2017 relating to the financial statements, which appears in Aeglea BioTherapeutics Inc.’s Annual Report on Form10-K for the year ended December 31, 2016. We also consent to the reference to us under the heading “Experts” in such Registration Statement.
/s/ PricewaterhouseCoopers LLP
Austin, Texas
May 23, 2017
