EXHIBIT 23.1
CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We hereby consent to the incorporation by reference in this Registration Statement onForm S-3 of Aeglea BioTherapeutics, Inc. of our report dated March 13, 2018 relating to the financial statements, which appears in Aeglea BioTherapeutics, Inc.’s Annual Report onForm 10-K for the year ended December 31, 2017. We also consent to the reference to us under the heading “Experts,” in such Registration Statement.
/s/ PricewaterhouseCoopers LLP
Austin, TX
December 21, 2018
